Rw1G001080

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003280
Physical Location & Seq
Reverse (-)
1594469 .. 1594936
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G001080.1

Sequence Viewer

Length: 468 bp
ATGGCGTATACAAAAAGCCTCGCGATCACCTCCACATTATTATTCATCATGTGGCTGGGGATTTTGGGAACATCACAAGCCACATCCCGCAGATTCAAAGAGAATCCAAATTCTACTCTTTTCCTAGAATTTGAGCGATGGATGGCAGCCTTCGACAAACATTACCCGAACGACGAAGAGAAGGAGAGGCGTTTCGCAATCTTCAAGAACAAGTTGGAGTGGGTCAAGAACTTCAACAAGCAAGGGAATCACTCTTTTACGGTAGGAATCAATCAATTTTCTGATATGACCCCTGATGAATTCCGGCTATGTGCTACTGGCCGCCGCCGCCACCCCAGCTTAAGCTCTAGTGTGGTCGACTCCTCCAGGTGGCTGATCTGGGTTCTGCCTTTATTTGGCATTATAGCCGTGTTGATGTGTGGTACGGCTATGGAGCAGGGTTGGTTTGCTTGGAAGTCCGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04210 ko04612 ko04621 ko04924 map04140 map04142 map04210 map04612 map04621 map04924
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

155

Amino Acids

18.04

Weight (kDa)

9.26

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 44 - 101 1.1e-20 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 8, 357
AccII CGCG 1 cut(s) 23
AciI CCGC 4 cut(s) 88, 322, 325, 328
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 3 cut(s) 109, 128, 299
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 2 cut(s) 369, 395
AflII CTTAAG 1 cut(s) 340
AgsI TTSAA 3 cut(s) 97, 205, 235
AjnI CCWGG 1 cut(s) 365
AluBI AGCT 2 cut(s) 339, 345
AluI AGCT 2 cut(s) 339, 345
Alw26I GTCTC 1 cut(s) 455
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 3 cut(s) 109, 128, 299
AsuHPI GGTGA 1 cut(s) 19
BbvI GCAGC 1 cut(s) 158
BccI CCATC 2 cut(s) 132, 136
BceAI ACGGC 2 cut(s) 392, 441
BciT130I CCWGG 1 cut(s) 367
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 2 cut(s) 125, 348
BfrI CTTAAG 1 cut(s) 340
BisI GCNGC 4 cut(s) 147, 322, 325, 328
BlsI GCNGC 4 cut(s) 148, 323, 326, 329
Bme1390I CCNGG 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 349
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 395
Bse1I ACTGG 1 cut(s) 322
BseBI CCWGG 1 cut(s) 367
BseGI GGATG 2 cut(s) 83, 147
BseLI CCNNNNNNNGG 2 cut(s) 369, 395
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 1 cut(s) 352
BseXI GCAGC 1 cut(s) 158
BseYI CCCAGC 2 cut(s) 55, 335
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 1 cut(s) 321
BsiSI CCGG 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 369, 395
BsmAI GTCTC 1 cut(s) 455
BsnI GGCC 1 cut(s) 321
Bsp143I GATC 2 cut(s) 24, 375
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 4 cut(s) 88, 322, 325, 328
BspANI GGCC 1 cut(s) 321
BspFNI CGCG 1 cut(s) 23
BspTI CTTAAG 1 cut(s) 340
BsrI ACTGG 1 cut(s) 322
BssMI GATC 2 cut(s) 24, 375
BssNAI GTATAC 1 cut(s) 9
Bst1107I GTATAC 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 171
BstAFI CTTAAG 1 cut(s) 340
BstF5I GGATG 2 cut(s) 83, 147
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 2 cut(s) 27, 378
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 2 cut(s) 24, 375
BstMWI GCNNNNNNNGC 2 cut(s) 327, 336
BstNI CCWGG 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 365
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 158
BstZ17I GTATAC 1 cut(s) 9
BsuRI GGCC 1 cut(s) 321
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 2 cut(s) 83, 147
BtuMI TCGCGA 1 cut(s) 23
Csp6I GTAC 1 cut(s) 423
CviAII CATG 1 cut(s) 49
CviQI GTAC 1 cut(s) 423
DpnI GATC 2 cut(s) 26, 377
DpnII GATC 2 cut(s) 24, 375
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 365
FaeI CATG 1 cut(s) 52
FaiI YATR 6 cut(s) 9, 50, 287, 310, 404, 431
FatI CATG 1 cut(s) 48
FauI CCCGC 1 cut(s) 95
FblI GTMKAC 2 cut(s) 8, 357
Fnu4HI GCNGC 4 cut(s) 147, 322, 325, 328
FokI GGATG 2 cut(s) 70, 154
Fsp4HI GCNGC 4 cut(s) 147, 322, 325, 328
FspBI CTAG 2 cut(s) 125, 348
GluI GCNGC 4 cut(s) 147, 322, 325, 328
GsaI CCCAGC 2 cut(s) 59, 339
GsuI CTGGAG 1 cut(s) 349
HaeIII GGCC 1 cut(s) 321
HapII CCGG 1 cut(s) 304
Hin1II CATG 1 cut(s) 52
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 5 cut(s) 93, 103, 247, 267, 359
HpaII CCGG 1 cut(s) 304
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 9, 358
Hpy188I TCNGA 2 cut(s) 283, 460
Hpy188III TCNNGA 3 cut(s) 22, 205, 226
Hpy8I GTNNAC 2 cut(s) 9, 358
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 2 cut(s) 160, 175
HpyCH4III ACNGT 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 2 cut(s) 327, 336
Hsp92II CATG 1 cut(s) 52
Kzo9I GATC 2 cut(s) 24, 375
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 9 cut(s) 41, 303, 306, 317, 349, 352, 364, 379, 422
Lsp1109I GCAGC 1 cut(s) 158
MaeI CTAG 2 cut(s) 125, 348
MalI GATC 2 cut(s) 26, 377
MboI GATC 2 cut(s) 24, 375
MboII GAAGA 2 cut(s) 188, 193
MluCI AATT 4 cut(s) 109, 128, 275, 299
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 4 cut(s) 29, 40, 180, 373
MseI TTAA 1 cut(s) 341
MspCI CTTAAG 1 cut(s) 340
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 1 cut(s) 367
MvaI CCWGG 1 cut(s) 367
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 327, 336
NdeII GATC 2 cut(s) 24, 375
NlaIII CATG 1 cut(s) 52
NruI TCGCGA 1 cut(s) 23
PfeI GAWTC 4 cut(s) 93, 103, 247, 267
PkrI GCNGC 4 cut(s) 148, 323, 326, 329
PleI GAGTC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 365
PspFI CCCAGC 2 cut(s) 55, 335
PspGI CCWGG 1 cut(s) 365
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 341
SatI GCNGC 4 cut(s) 147, 322, 325, 328
Sau3AI GATC 2 cut(s) 24, 375
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 367
SetI ASST 4 cut(s) 32, 341, 347, 371
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
Sse9I AATT 4 cut(s) 109, 128, 275, 299
SsiI CCGC 4 cut(s) 88, 322, 325, 328
SspMI CTAG 2 cut(s) 125, 348
StyD4I CCNGG 1 cut(s) 365
TaaI ACNGT 1 cut(s) 262
TaqI TCGA 2 cut(s) 153, 357
TasI AATT 4 cut(s) 109, 128, 275, 299
TauI GCSGC 3 cut(s) 324, 327, 330
TfiI GAWTC 4 cut(s) 93, 103, 247, 267
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 34, 312
Vha464I CTTAAG 1 cut(s) 340
XapI RAATTY 3 cut(s) 109, 128, 299
XmiI GTMKAC 2 cut(s) 8, 357
XspI CTAG 2 cut(s) 125, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.