Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1735 |
| AccBSI | CCGCTC | 1 cut(s) | 660 |
| AccI | GTMKAC | 1 cut(s) | 1531 |
| AccII | CGCG | 2 cut(s) | 136, 1011 |
| AccIII | TCCGGA | 1 cut(s) | 2290 |
| AciI | CCGC | 5 cut(s) | 355, 658, 1011, 1555, 2307 |
| AclWI | GGATC | 3 cut(s) | 335, 945, 1826 |
| AcoI | YGGCCR | 4 cut(s) | 158, 571, 579, 655 |
| AcsI | RAATTY | 1 cut(s) | 849 |
| AcuI | CTGAAG | 5 cut(s) | 201, 795, 1778, 2031, 2281 |
| AfaI | GTAC | 6 cut(s) | 1107, 1319, 1381, 1769, 2302, 2325 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 357, 526, 899, 911, 1735, 2051, 2260, 2261 |
| AflIII | ACRYGT | 1 cut(s) | 23 |
| AgsI | TTSAA | 6 cut(s) | 988, 1050, 1204, 2003, 2086, 2236 |
| AjnI | CCWGG | 2 cut(s) | 63, 666 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1776, 1808 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 873, 905, 2054, 2086 |
| Alw21I | GWGCWC | 2 cut(s) | 549, 1167 |
| Alw26I | GTCTC | 4 cut(s) | 979, 1034, 1586, 1745 |
| AlwI | GGATC | 3 cut(s) | 335, 945, 1826 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 596 |
| Ama87I | CYCGRG | 1 cut(s) | 2390 |
| Aor13HI | TCCGGA | 1 cut(s) | 2290 |
| AoxI | GGCC | 8 cut(s) | 158, 211, 571, 579, 655, 2207, 2264, 2338 |
| ApoI | RAATTY | 1 cut(s) | 849 |
| AseI | ATTAAT | 1 cut(s) | 1404 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 451, 2163 |
| AspLEI | GCGC | 5 cut(s) | 91, 138, 800, 917, 1016 |
| AspS9I | GGNCC | 6 cut(s) | 212, 470, 902, 1828, 2054, 2264 |
| AsuC2I | CCSGG | 1 cut(s) | 1956 |
| AsuHPI | GGTGA | 2 cut(s) | 80, 782 |
| AsuII | TTCGAA | 2 cut(s) | 1939, 2099 |
| AvaI | CYCGRG | 1 cut(s) | 2390 |
| AvaII | GGWCC | 4 cut(s) | 470, 902, 1828, 2054 |
| AxyI | CCTNAGG | 2 cut(s) | 765, 2211 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2284, 2317 |
| BalI | TGGCCA | 1 cut(s) | 581 |
| BanII | GRGCYC | 3 cut(s) | 549, 1167, 1994 |
| BauI | CACGAG | 1 cut(s) | 174 |
| BbsI | GAAGAC | 7 cut(s) | 52, 296, 478, 995, 1234, 1482, 1873 |
| Bbv12I | GWGCWC | 2 cut(s) | 549, 1167 |
| BccI | CCATC | 7 cut(s) | 37, 110, 461, 742, 1498, 1733, 2027 |
| BceAI | ACGGC | 1 cut(s) | 1460 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1592, 1626 |
| BciT130I | CCWGG | 2 cut(s) | 65, 668 |
| BclI | TGATCA | 2 cut(s) | 1207, 1776 |
| BcnI | CCSGG | 1 cut(s) | 1956 |
| BcoDI | GTCTC | 4 cut(s) | 979, 1034, 1586, 1745 |
| BfaI | CTAG | 4 cut(s) | 323, 1124, 1653, 1838 |
| BfmI | CTRYAG | 3 cut(s) | 1080, 1266, 2145 |
| BfoI | RGCGCY | 2 cut(s) | 918, 1017 |
| BlpI | GCTNAGC | 2 cut(s) | 543, 1947 |
| Bme1390I | CCNGG | 3 cut(s) | 65, 668, 1956 |
| Bme18I | GGWCC | 4 cut(s) | 470, 902, 1828, 2054 |
| BmeT110I | CYCGRG | 1 cut(s) | 2390 |
| BmgT120I | GGNCC | 6 cut(s) | 212, 470, 902, 1828, 2054, 2264 |
| BmiI | GGNNCC | 5 cut(s) | 472, 1136, 1388, 1744, 2265 |
| BmrFI | CCNGG | 3 cut(s) | 65, 668, 1956 |
| BmsI | GCATC | 2 cut(s) | 1960, 2318 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1833 |
| BpiI | GAAGAC | 7 cut(s) | 52, 296, 478, 995, 1234, 1482, 1873 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1995 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 543, 1947 |
| Bpu14I | TTCGAA | 2 cut(s) | 1939, 2099 |
| BpuEI | CTTGAG | 2 cut(s) | 533, 961 |
| BpuMI | CCSGG | 1 cut(s) | 1956 |
| BsaI | GGTCTC | 2 cut(s) | 1034, 1586 |
| BsaJI | CCNNGG | 7 cut(s) | 64, 666, 804, 1375, 1423, 1831, 2391 |
| BsaWI | WCCGGW | 1 cut(s) | 2290 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2054, 2084 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 357, 526, 899, 911, 1735, 2051, 2260, 2261 |
| Bse118I | RCCGGY | 1 cut(s) | 1288 |
| Bse1I | ACTGG | 2 cut(s) | 367, 776 |
| Bse21I | CCTNAGG | 2 cut(s) | 765, 2211 |
| Bse3DI | GCAATG | 4 cut(s) | 1467, 1707, 1873, 2073 |
| BseAI | TCCGGA | 1 cut(s) | 2290 |
| BseBI | CCWGG | 2 cut(s) | 65, 668 |
| BseDI | CCNNGG | 7 cut(s) | 64, 666, 804, 1375, 1423, 1831, 2391 |
| BseGI | GGATG | 4 cut(s) | 701, 753, 1744, 1951 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 357, 526, 899, 911, 1735, 2051, 2260, 2261 |
| BseMI | GCAATG | 4 cut(s) | 1467, 1707, 1873, 2073 |
| BseMII | CTCAG | 8 cut(s) | 391, 534, 756, 1031, 1598, 1938, 1986, 2225 |
| BseNI | ACTGG | 2 cut(s) | 367, 776 |
| BseRI | GAGGAG | 2 cut(s) | 2075, 2138 |
| BseYI | CCCAGC | 1 cut(s) | 824 |
| BsgI | GTGCAG | 2 cut(s) | 773, 1657 |
| Bsh1236I | CGCG | 2 cut(s) | 136, 1011 |
| BshFI | GGCC | 8 cut(s) | 160, 213, 573, 581, 657, 2209, 2266, 2340 |
| BsiHKAI | GWGCWC | 2 cut(s) | 549, 1167 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2390 |
| BsiSI | CCGG | 4 cut(s) | 358, 1289, 1955, 2291 |
| BslFI | GGGAC | 2 cut(s) | 334, 1441 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 357, 526, 899, 911, 1735, 2051, 2260, 2261 |
| BsmAI | GTCTC | 4 cut(s) | 979, 1034, 1586, 1745 |
| BsmBI | CGTCTC | 1 cut(s) | 1745 |
| BsmFI | GGGAC | 2 cut(s) | 334, 1441 |
| BsmI | GAATGC | 4 cut(s) | 138, 534, 869, 1804 |
| BsnI | GGCC | 8 cut(s) | 160, 213, 573, 581, 657, 2209, 2266, 2340 |
| Bso31I | GGTCTC | 2 cut(s) | 1034, 1586 |
| BsoBI | CYCGRG | 1 cut(s) | 2390 |
| Bsp119I | TTCGAA | 2 cut(s) | 1939, 2099 |
| Bsp1286I | GDGCHC | 3 cut(s) | 549, 1167, 1994 |
| Bsp13I | TCCGGA | 1 cut(s) | 2290 |
| Bsp143I | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 543, 1947 |
| Bsp19I | CCATGG | 1 cut(s) | 1375 |
| BspACI | CCGC | 5 cut(s) | 355, 658, 1011, 1555, 2307 |
| BspANI | GGCC | 8 cut(s) | 160, 213, 573, 581, 657, 2209, 2266, 2340 |
| BspCNI | CTCAG | 8 cut(s) | 390, 535, 757, 1030, 1599, 1939, 1987, 2224 |
| BspEI | TCCGGA | 1 cut(s) | 2290 |
| BspFNI | CGCG | 2 cut(s) | 136, 1011 |
| BspHI | TCATGA | 1 cut(s) | 1140 |
| BspLI | GGNNCC | 5 cut(s) | 472, 1136, 1388, 1744, 2265 |
| BspMAI | CTGCAG | 1 cut(s) | 2149 |
| BspPI | GGATC | 3 cut(s) | 335, 945, 1826 |
| BspT104I | TTCGAA | 2 cut(s) | 1939, 2099 |
| BspTNI | GGTCTC | 2 cut(s) | 1034, 1586 |
| BsrBI | CCGCTC | 1 cut(s) | 660 |
| BsrDI | GCAATG | 4 cut(s) | 1467, 1707, 1873, 2073 |
| BsrFI | RCCGGY | 1 cut(s) | 1288 |
| BsrI | ACTGG | 2 cut(s) | 367, 776 |
| BssAI | RCCGGY | 1 cut(s) | 1288 |
| BssECI | CCNNGG | 7 cut(s) | 64, 666, 804, 1375, 1423, 1831, 2391 |
| BssMI | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| BssNAI | GTATAC | 1 cut(s) | 1532 |
| BssSI | CACGAG | 1 cut(s) | 174 |
| BssT1I | CCWWGG | 2 cut(s) | 804, 1375 |
| Bst1107I | GTATAC | 1 cut(s) | 1532 |
| Bst2BI | CACGAG | 1 cut(s) | 174 |
| Bst2UI | CCWGG | 2 cut(s) | 65, 668 |
| Bst4CI | ACNGT | 4 cut(s) | 19, 277, 1832, 1888 |
| Bst6I | CTCTTC | 3 cut(s) | 314, 1100, 1648 |
| BstBI | TTCGAA | 2 cut(s) | 1939, 2099 |
| BstC8I | GCNNGC | 5 cut(s) | 532, 913, 1290, 1658, 1864 |
| BstDSI | CCRYGG | 3 cut(s) | 1375, 1423, 1831 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2049 |
| BstF5I | GGATG | 4 cut(s) | 701, 753, 1744, 1951 |
| BstFNI | CGCG | 2 cut(s) | 136, 1011 |
| BstH2I | RGCGCY | 2 cut(s) | 918, 1017 |
| BstHHI | GCGC | 5 cut(s) | 91, 138, 800, 917, 1016 |
| BstKTI | GATC | 6 cut(s) | 343, 940, 1210, 1779, 1821, 1915 |
| BstMAI | GTCTC | 4 cut(s) | 979, 1034, 1586, 1745 |
| BstMBI | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 587, 806 |
| BstNI | CCWGG | 2 cut(s) | 65, 668 |
| BstNSI | RCATGY | 2 cut(s) | 27, 1660 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1833 |
| BstSCI | CCNGG | 3 cut(s) | 63, 666, 1954 |
| BstSFI | CTRYAG | 3 cut(s) | 1080, 1266, 2145 |
| BstUI | CGCG | 2 cut(s) | 136, 1011 |
| BstV2I | GAAGAC | 7 cut(s) | 52, 296, 478, 995, 1234, 1482, 1873 |
| BstX2I | RGATCY | 2 cut(s) | 340, 937 |
| BstYI | RGATCY | 2 cut(s) | 340, 937 |
| BstZ17I | GTATAC | 1 cut(s) | 1532 |
| Bsu36I | CCTNAGG | 2 cut(s) | 765, 2211 |
| BsuRI | GGCC | 8 cut(s) | 160, 213, 573, 581, 657, 2209, 2266, 2340 |
| BtgI | CCRYGG | 3 cut(s) | 1375, 1423, 1831 |
| BtsCI | GGATG | 4 cut(s) | 701, 753, 1744, 1951 |
| BtsIMutI | CAGTG | 5 cut(s) | 282, 561, 834, 1818, 1884 |
| Cac8I | GCNNGC | 5 cut(s) | 532, 913, 1290, 1658, 1864 |
| CaiI | CAGNNNCTG | 1 cut(s) | 596 |
| CciI | TCATGA | 1 cut(s) | 1140 |
| CfoI | GCGC | 5 cut(s) | 91, 138, 800, 917, 1016 |
| Cfr10I | RCCGGY | 1 cut(s) | 1288 |
| Cfr13I | GGNCC | 6 cut(s) | 212, 470, 902, 1828, 2054, 2264 |
| Csp6I | GTAC | 6 cut(s) | 1106, 1318, 1380, 1768, 2301, 2324 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 550, 585 |
| CviQI | GTAC | 6 cut(s) | 1106, 1318, 1380, 1768, 2301, 2324 |
| DpnI | GATC | 6 cut(s) | 342, 939, 1209, 1778, 1820, 1914 |
| DpnII | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| DraI | TTTAAA | 1 cut(s) | 742 |
| EaeI | YGGCCR | 4 cut(s) | 158, 571, 579, 655 |
| Eam1104I | CTCTTC | 3 cut(s) | 314, 1100, 1648 |
| EarI | CTCTTC | 3 cut(s) | 314, 1100, 1648 |
| EciI | GGCGGA | 1 cut(s) | 344 |
| Ecl136II | GAGCTC | 2 cut(s) | 547, 1165 |
| Eco130I | CCWWGG | 2 cut(s) | 804, 1375 |
| Eco147I | AGGCCT | 1 cut(s) | 2209 |
| Eco24I | GRGCYC | 3 cut(s) | 549, 1167, 1994 |
| Eco31I | GGTCTC | 2 cut(s) | 1034, 1586 |
| Eco47I | GGWCC | 4 cut(s) | 470, 902, 1828, 2054 |
| Eco53kI | GAGCTC | 2 cut(s) | 547, 1165 |
| Eco57I | CTGAAG | 5 cut(s) | 201, 795, 1778, 2031, 2281 |
| Eco81I | CCTNAGG | 2 cut(s) | 765, 2211 |
| Eco88I | CYCGRG | 1 cut(s) | 2390 |
| EcoICRI | GAGCTC | 2 cut(s) | 547, 1165 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2049 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2264 |
| EcoRI | GAATTC | 1 cut(s) | 849 |
| EcoRII | CCWGG | 2 cut(s) | 63, 666 |
| EcoT14I | CCWWGG | 2 cut(s) | 804, 1375 |
| EcoT22I | ATGCAT | 1 cut(s) | 1662 |
| EcoT38I | GRGCYC | 3 cut(s) | 549, 1167, 1994 |
| ErhI | CCWWGG | 2 cut(s) | 804, 1375 |
| Esp3I | CGTCTC | 1 cut(s) | 1745 |
| FaqI | GGGAC | 2 cut(s) | 334, 1441 |
| FbaI | TGATCA | 2 cut(s) | 1207, 1776 |
| FblI | GTMKAC | 1 cut(s) | 1531 |
| FokI | GGATG | 4 cut(s) | 708, 760, 1751, 1938 |
| FriOI | GRGCYC | 3 cut(s) | 549, 1167, 1994 |
| FspBI | CTAG | 4 cut(s) | 323, 1124, 1653, 1838 |
| GlaI | GCGC | 5 cut(s) | 90, 137, 799, 916, 1015 |
| GsaI | CCCAGC | 1 cut(s) | 828 |
| HaeII | RGCGCY | 2 cut(s) | 918, 1017 |
| HaeIII | GGCC | 8 cut(s) | 160, 213, 573, 581, 657, 2209, 2266, 2340 |
| HapII | CCGG | 4 cut(s) | 358, 1289, 1955, 2291 |
| HhaI | GCGC | 5 cut(s) | 91, 138, 800, 917, 1016 |
| Hin6I | GCGC | 5 cut(s) | 89, 136, 798, 915, 1014 |
| HinP1I | GCGC | 5 cut(s) | 89, 136, 798, 915, 1014 |
| HincII | GTYRAC | 1 cut(s) | 2283 |
| HindII | GTYRAC | 1 cut(s) | 2283 |
| HinfI | GANTC | 9 cut(s) | 57, 619, 1359, 1609, 1756, 1874, 1936, 2159, 2399 |
| HpaII | CCGG | 4 cut(s) | 358, 1289, 1955, 2291 |
| HphI | GGTGA | 2 cut(s) | 80, 782 |
| Hpy166II | GTNNAC | 7 cut(s) | 773, 1510, 1532, 1828, 1978, 2029, 2283 |
| Hpy188I | TCNGA | 4 cut(s) | 1064, 1358, 1797, 2404 |
| Hpy188III | TCNNGA | 9 cut(s) | 80, 764, 1115, 1141, 1363, 1916, 2168, 2242, 2291 |
| Hpy8I | GTNNAC | 7 cut(s) | 773, 1510, 1532, 1828, 1978, 2029, 2283 |
| HpyAV | CCTTC | 7 cut(s) | 225, 526, 893, 1261, 2055, 2199, 2368 |
| HpyCH4III | ACNGT | 4 cut(s) | 19, 277, 1832, 1888 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 587, 806 |
| HspAI | GCGC | 5 cut(s) | 89, 136, 798, 915, 1014 |
| Kpn2I | TCCGGA | 1 cut(s) | 2290 |
| KroI | GCCGGC | 1 cut(s) | 1288 |
| KroNI | GCCGGC | 1 cut(s) | 1290 |
| Ksp22I | TGATCA | 2 cut(s) | 1207, 1776 |
| Kzo9I | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| LmnI | GCTCC | 4 cut(s) | 96, 1162, 1359, 1474 |
| LweI | GCATC | 2 cut(s) | 1960, 2318 |
| MaeI | CTAG | 4 cut(s) | 323, 1124, 1653, 1838 |
| MaeIII | GTNAC | 6 cut(s) | 19, 124, 227, 832, 2183, 2246 |
| MalI | GATC | 6 cut(s) | 342, 939, 1209, 1778, 1820, 1914 |
| MbiI | CCGCTC | 1 cut(s) | 660 |
| MboI | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| MflI | RGATCY | 2 cut(s) | 340, 937 |
| MhlI | GDGCHC | 3 cut(s) | 549, 1167, 1994 |
| MlsI | TGGCCA | 1 cut(s) | 581 |
| MluNI | TGGCCA | 1 cut(s) | 581 |
| MlyI | GAGTC | 4 cut(s) | 66, 1353, 1618, 1750 |
| MmeI | TCCRAC | 3 cut(s) | 175, 1381, 1716 |
| Mox20I | TGGCCA | 1 cut(s) | 581 |
| Mph1103I | ATGCAT | 1 cut(s) | 1662 |
| MroI | TCCGGA | 1 cut(s) | 2290 |
| MroNI | GCCGGC | 1 cut(s) | 1288 |
| MroXI | GAANNNNTTC | 2 cut(s) | 451, 2163 |
| MscI | TGGCCA | 1 cut(s) | 581 |
| MseI | TTAA | 7 cut(s) | 557, 600, 741, 1176, 1404, 1479, 1967 |
| MslI | CAYNNNNRTG | 1 cut(s) | 494 |
| Msp20I | TGGCCA | 1 cut(s) | 581 |
| MspA1I | CMGCKG | 5 cut(s) | 596, 824, 1555, 1606, 2412 |
| MspI | CCGG | 4 cut(s) | 358, 1289, 1955, 2291 |
| MspR9I | CCNGG | 3 cut(s) | 65, 668, 1956 |
| Mva1269I | GAATGC | 4 cut(s) | 138, 534, 869, 1804 |
| MvaI | CCWGG | 2 cut(s) | 65, 668 |
| MvnI | CGCG | 2 cut(s) | 136, 1011 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 587, 806 |
| NaeI | GCCGGC | 1 cut(s) | 1290 |
| NciI | CCSGG | 1 cut(s) | 1956 |
| NcoI | CCATGG | 1 cut(s) | 1375 |
| NdeII | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| NgoMIV | GCCGGC | 1 cut(s) | 1288 |
| NlaIV | GGNNCC | 5 cut(s) | 472, 1136, 1388, 1744, 2265 |
| NmuCI | GTSAC | 3 cut(s) | 227, 832, 2246 |
| NsiI | ATGCAT | 1 cut(s) | 1662 |
| NspI | RCATGY | 2 cut(s) | 27, 1660 |
| NspV | TTCGAA | 2 cut(s) | 1939, 2099 |
| PaeI | GCATGC | 1 cut(s) | 1660 |
| PagI | TCATGA | 1 cut(s) | 1140 |
| PceI | AGGCCT | 1 cut(s) | 2209 |
| PciI | ACATGT | 1 cut(s) | 23 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2388 |
| PctI | GAATGC | 4 cut(s) | 138, 534, 869, 1804 |
| PdiI | GCCGGC | 1 cut(s) | 1290 |
| PdmI | GAANNNNTTC | 2 cut(s) | 451, 2163 |
| PfeI | GAWTC | 5 cut(s) | 619, 1874, 1936, 2159, 2399 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1735 |
| PleI | GAGTC | 4 cut(s) | 65, 1353, 1617, 1750 |
| PpsI | GAGTC | 4 cut(s) | 65, 1353, 1617, 1750 |
| PscI | ACATGT | 1 cut(s) | 23 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1833 |
| PshBI | ATTAAT | 1 cut(s) | 1404 |
| Psp124BI | GAGCTC | 2 cut(s) | 549, 1167 |
| Psp6I | CCWGG | 2 cut(s) | 63, 666 |
| PspFI | CCCAGC | 1 cut(s) | 824 |
| PspGI | CCWGG | 2 cut(s) | 63, 666 |
| PspN4I | GGNNCC | 5 cut(s) | 472, 1136, 1388, 1744, 2265 |
| PspPI | GGNCC | 6 cut(s) | 212, 470, 902, 1828, 2054, 2264 |
| PstI | CTGCAG | 1 cut(s) | 2149 |
| PstNI | CAGNNNCTG | 1 cut(s) | 596 |
| PsuI | RGATCY | 2 cut(s) | 340, 937 |
| PvuII | CAGCTG | 4 cut(s) | 596, 824, 1606, 2412 |
| RsaI | GTAC | 6 cut(s) | 1107, 1319, 1381, 1769, 2302, 2325 |
| RsaNI | GTAC | 6 cut(s) | 1106, 1318, 1380, 1768, 2301, 2324 |
| RseI | CAYNNNNRTG | 1 cut(s) | 494 |
| SacI | GAGCTC | 2 cut(s) | 549, 1167 |
| SaqAI | TTAA | 7 cut(s) | 557, 600, 741, 1176, 1404, 1479, 1967 |
| Sau3AI | GATC | 6 cut(s) | 340, 937, 1207, 1776, 1818, 1912 |
| Sau96I | GGNCC | 6 cut(s) | 212, 470, 902, 1828, 2054, 2264 |
| SchI | GAGTC | 4 cut(s) | 66, 1353, 1618, 1750 |
| ScrFI | CCNGG | 3 cut(s) | 65, 668, 1956 |
| SduI | GDGCHC | 3 cut(s) | 549, 1167, 1994 |
| SfaNI | GCATC | 2 cut(s) | 1960, 2318 |
| SfcI | CTRYAG | 3 cut(s) | 1080, 1266, 2145 |
| SfuI | TTCGAA | 2 cut(s) | 1939, 2099 |
| SinI | GGWCC | 4 cut(s) | 470, 902, 1828, 2054 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 494 |
| SmlI | CTYRAG | 2 cut(s) | 548, 976 |
| SmoI | CTYRAG | 2 cut(s) | 548, 976 |
| SphI | GCATGC | 1 cut(s) | 1660 |
| SseBI | AGGCCT | 1 cut(s) | 2209 |
| SsiI | CCGC | 5 cut(s) | 355, 658, 1011, 1555, 2307 |
| SspI | AATATT | 1 cut(s) | 1500 |
| SspMI | CTAG | 4 cut(s) | 323, 1124, 1653, 1838 |
| SstI | GAGCTC | 2 cut(s) | 549, 1167 |
| StuI | AGGCCT | 1 cut(s) | 2209 |
| StyD4I | CCNGG | 3 cut(s) | 63, 666, 1954 |
| StyI | CCWWGG | 2 cut(s) | 804, 1375 |
| TaaI | ACNGT | 4 cut(s) | 19, 277, 1832, 1888 |
| TaqI | TCGA | 9 cut(s) | 50, 730, 1413, 1612, 1759, 1939, 2099, 2203, 2372 |
| TaqII | GACCGA | 1 cut(s) | 2302 |
| TatI | WGTACW | 3 cut(s) | 1317, 1767, 2323 |
| TauI | GCSGC | 2 cut(s) | 660, 1014 |
| TfiI | GAWTC | 5 cut(s) | 619, 1874, 1936, 2159, 2399 |
| Tru1I | TTAA | 7 cut(s) | 557, 600, 741, 1176, 1404, 1479, 1967 |
| Tru9I | TTAA | 7 cut(s) | 557, 600, 741, 1176, 1404, 1479, 1967 |
| TscAI | CASTG | 5 cut(s) | 282, 568, 841, 1818, 1891 |
| TseFI | GTSAC | 3 cut(s) | 227, 832, 2246 |
| Tsp45I | GTSAC | 3 cut(s) | 227, 832, 2246 |
| TspDTI | ATGAA | 7 cut(s) | 53, 478, 842, 1157, 1243, 1497, 2062 |
| TspRI | CASTG | 5 cut(s) | 282, 568, 841, 1818, 1891 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1735 |
| VpaK11BI | GGWCC | 4 cut(s) | 470, 902, 1828, 2054 |
| VspI | ATTAAT | 1 cut(s) | 1404 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2049 |
| XapI | RAATTY | 1 cut(s) | 849 |
| XceI | RCATGY | 2 cut(s) | 27, 1660 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1736 |
| XmiI | GTMKAC | 1 cut(s) | 1531 |
| XmnI | GAANNNNTTC | 2 cut(s) | 451, 2163 |
| XspI | CTAG | 4 cut(s) | 323, 1124, 1653, 1838 |
| Zsp2I | ATGCAT | 1 cut(s) | 1662 |