Reverse transcriptase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 34 |
| AclWI | GGATC | 2 cut(s) | 151, 164 |
| AcsI | RAATTY | 2 cut(s) | 77, 262 |
| AgsI | TTSAA | 2 cut(s) | 200, 224 |
| AluBI | AGCT | 2 cut(s) | 95, 227 |
| AluI | AGCT | 2 cut(s) | 95, 227 |
| AlwI | GGATC | 2 cut(s) | 151, 164 |
| AoxI | GGCC | 1 cut(s) | 98 |
| ApoI | RAATTY | 2 cut(s) | 77, 262 |
| AspS9I | GGNCC | 2 cut(s) | 99, 171 |
| AsuHPI | GGTGA | 3 cut(s) | 80, 86, 111 |
| AvaII | GGWCC | 1 cut(s) | 171 |
| BamHI | GGATCC | 1 cut(s) | 156 |
| BccI | CCATC | 2 cut(s) | 5, 65 |
| BfaI | CTAG | 1 cut(s) | 83 |
| Bme18I | GGWCC | 1 cut(s) | 171 |
| BmgT120I | GGNCC | 2 cut(s) | 99, 171 |
| BmiI | GGNNCC | 1 cut(s) | 158 |
| BmsI | GCATC | 1 cut(s) | 258 |
| BpuEI | CTTGAG | 2 cut(s) | 131, 293 |
| Bse3DI | GCAATG | 1 cut(s) | 70 |
| BseMI | GCAATG | 1 cut(s) | 70 |
| BseRI | GAGGAG | 1 cut(s) | 290 |
| BseYI | CCCAGC | 1 cut(s) | 47 |
| BshFI | GGCC | 1 cut(s) | 100 |
| BsnI | GGCC | 1 cut(s) | 100 |
| Bsp143I | GATC | 1 cut(s) | 156 |
| BspANI | GGCC | 1 cut(s) | 100 |
| BspLI | GGNNCC | 1 cut(s) | 158 |
| BspPI | GGATC | 2 cut(s) | 151, 164 |
| BsrDI | GCAATG | 1 cut(s) | 70 |
| BssMI | GATC | 1 cut(s) | 156 |
| Bst4CI | ACNGT | 1 cut(s) | 8 |
| BstC8I | GCNNGC | 1 cut(s) | 61 |
| BstKTI | GATC | 1 cut(s) | 159 |
| BstMBI | GATC | 1 cut(s) | 156 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 23 |
| BstX2I | RGATCY | 1 cut(s) | 156 |
| BstYI | RGATCY | 1 cut(s) | 156 |
| BsuRI | GGCC | 1 cut(s) | 100 |
| Cac8I | GCNNGC | 1 cut(s) | 61 |
| Cfr13I | GGNCC | 2 cut(s) | 99, 171 |
| CviAII | CATG | 1 cut(s) | 128 |
| CviJI | RGCY | 5 cut(s) | 17, 26, 95, 100, 227 |
| CviKI_1 | RGCY | 5 cut(s) | 17, 26, 95, 100, 227 |
| DpnI | GATC | 1 cut(s) | 158 |
| DpnII | GATC | 1 cut(s) | 156 |
| Eco47I | GGWCC | 1 cut(s) | 171 |
| EcoO109I | RGGNCCY | 1 cut(s) | 171 |
| EcoRI | GAATTC | 1 cut(s) | 77 |
| FaeI | CATG | 1 cut(s) | 131 |
| FaiI | YATR | 6 cut(s) | 39, 105, 129, 183, 189, 260 |
| FatI | CATG | 1 cut(s) | 127 |
| FblI | GTMKAC | 1 cut(s) | 34 |
| FspBI | CTAG | 1 cut(s) | 83 |
| GsaI | CCCAGC | 1 cut(s) | 51 |
| HaeIII | GGCC | 1 cut(s) | 100 |
| Hin1II | CATG | 1 cut(s) | 131 |
| HphI | GGTGA | 3 cut(s) | 80, 86, 111 |
| Hpy166II | GTNNAC | 1 cut(s) | 35 |
| Hpy8I | GTNNAC | 1 cut(s) | 35 |
| HpyCH4III | ACNGT | 1 cut(s) | 8 |
| HpyCH4V | TGCA | 2 cut(s) | 63, 143 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 23 |
| Hsp92II | CATG | 1 cut(s) | 131 |
| Kzo9I | GATC | 1 cut(s) | 156 |
| LpnPI | CCDG | 1 cut(s) | 33 |
| LweI | GCATC | 1 cut(s) | 258 |
| MaeI | CTAG | 1 cut(s) | 83 |
| MalI | GATC | 1 cut(s) | 158 |
| MboI | GATC | 1 cut(s) | 156 |
| MflI | RGATCY | 1 cut(s) | 156 |
| MluCI | AATT | 3 cut(s) | 77, 184, 262 |
| MnlI | CCTC | 2 cut(s) | 145, 268 |
| MseI | TTAA | 2 cut(s) | 282, 293 |
| MslI | CAYNNNNRTG | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 23 |
| NdeII | GATC | 1 cut(s) | 156 |
| NlaIII | CATG | 1 cut(s) | 131 |
| NlaIV | GGNNCC | 1 cut(s) | 158 |
| NmeAIII | GCCGAG | 1 cut(s) | 76 |
| PpuMI | RGGWCCY | 1 cut(s) | 171 |
| Psp5II | RGGWCCY | 1 cut(s) | 171 |
| PspFI | CCCAGC | 1 cut(s) | 47 |
| PspN4I | GGNNCC | 1 cut(s) | 158 |
| PspPI | GGNCC | 2 cut(s) | 99, 171 |
| PspPPI | RGGWCCY | 1 cut(s) | 171 |
| PsuI | RGATCY | 1 cut(s) | 156 |
| RseI | CAYNNNNRTG | 1 cut(s) | 42 |
| SaqAI | TTAA | 2 cut(s) | 282, 293 |
| Sau3AI | GATC | 1 cut(s) | 156 |
| Sau96I | GGNCC | 2 cut(s) | 99, 171 |
| SetI | ASST | 5 cut(s) | 91, 97, 124, 176, 229 |
| SfaNI | GCATC | 1 cut(s) | 258 |
| SinI | GGWCC | 1 cut(s) | 171 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 42 |
| SmlI | CTYRAG | 2 cut(s) | 146, 272 |
| SmoI | CTYRAG | 2 cut(s) | 146, 272 |
| Sse9I | AATT | 3 cut(s) | 77, 184, 262 |
| SspI | AATATT | 1 cut(s) | 250 |
| SspMI | CTAG | 1 cut(s) | 83 |
| TaaI | ACNGT | 1 cut(s) | 8 |
| TasI | AATT | 3 cut(s) | 77, 184, 262 |
| Tru1I | TTAA | 2 cut(s) | 282, 293 |
| Tru9I | TTAA | 2 cut(s) | 282, 293 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| VpaK11BI | GGWCC | 1 cut(s) | 171 |
| XapI | RAATTY | 2 cut(s) | 77, 262 |
| XmiI | GTMKAC | 1 cut(s) | 34 |
| XspI | CTAG | 1 cut(s) | 83 |