MD12G1005800.v1.1

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003280
Physical Location & Seq
Forward (+)
681979 .. 682389
411 bp
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UTR
Exon/CDS
Intron
MD12G1005800.v1.1.491

Sequence Viewer

Length: 411 bp
ATGAGTTTTAATAATTGGAGAATCCTTTTTGCTACAGTATTTTTTGTACCAATACTGATCTGCGATGCAAGTTCTTTTCAAGCATTTGAAGAGTGGACCGCAGTGCACGGCCGTGTCTACTCCAGCGCTGAAGAACGAGAGCTGCGCCTCGCGATATTCAAAAACAACTTTGAATTTGTGGAGAAATTTAACAAGAATGGGAAAGGTTTTACCGTCGGCCTCAATCATTTTGCCGACTTGACTACTGAAGAGATCACCAACGGGTACTGCTGCAGATTAGTGCAGTCTGATTCGACCACTAACATGACAATCTCAGACAATGTTGTTGCTGTCGGAAACATTGACAACAGTACATGCAGCTGTTGGAGTAAATCAACCTTAAGTGGTCTACTACATGTAATAGGAATGTAA

Protein Analysis

137

Amino Acids

15.27

Weight (kDa)

5.26

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 29 - 85 1.1e-18 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 117, 388
AccII CGCG 1 cut(s) 152
AciI CCGC 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 2 cut(s) 173, 185
AcuI CTGAAG 2 cut(s) 150, 267
AfaI GTAC 3 cut(s) 48, 266, 352
AfeI AGCGCT 1 cut(s) 127
AflII CTTAAG 1 cut(s) 379
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 4 cut(s) 80, 89, 160, 173
AleI CACNNNNGTG 1 cut(s) 111
AluBI AGCT 2 cut(s) 142, 360
AluI AGCT 2 cut(s) 142, 360
Alw21I GWGCWC 1 cut(s) 108
Alw44I GTGCAC 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 127
AoxI GGCC 2 cut(s) 109, 217
ApaLI GTGCAC 1 cut(s) 104
ApeKI GCWGC 3 cut(s) 142, 270, 357
ApoI RAATTY 2 cut(s) 173, 185
AspLEI GCGC 2 cut(s) 128, 147
AspS9I GGNCC 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 3 cut(s) 129, 257, 369
BceAI ACGGC 2 cut(s) 96, 124
BfmI CTRYAG 2 cut(s) 33, 271
BfoI RGCGCY 1 cut(s) 129
BfrI CTTAAG 1 cut(s) 379
BisI GCNGC 3 cut(s) 143, 271, 358
BlsI GCNGC 3 cut(s) 144, 272, 359
Bme18I GGWCC 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 96
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 106
BseMII CTCAG 1 cut(s) 327
BseSI GKGCMC 1 cut(s) 108
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 3 cut(s) 129, 257, 369
BsgI GTGCAG 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 111, 219
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 108
BsnI GGCC 2 cut(s) 111, 219
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 57, 252
Bsp68I TCGCGA 1 cut(s) 152
BspACI CCGC 1 cut(s) 99
BspANI GGCC 2 cut(s) 111, 219
BspCNI CTCAG 1 cut(s) 326
BspFNI CGCG 1 cut(s) 152
BspMAI CTGCAG 1 cut(s) 275
BspTI CTTAAG 1 cut(s) 379
BssMI GATC 2 cut(s) 57, 252
Bst4CI ACNGT 3 cut(s) 37, 214, 350
Bst6I CTCTTC 2 cut(s) 84, 243
BstAFI CTTAAG 1 cut(s) 379
BstDEI CTNAG 1 cut(s) 313
BstFNI CGCG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 2 cut(s) 128, 147
BstKTI GATC 2 cut(s) 60, 255
BstMBI GATC 2 cut(s) 57, 252
BstMCI CGRYCG 1 cut(s) 112
BstNSI RCATGY 2 cut(s) 357, 398
BstSFI CTRYAG 2 cut(s) 33, 271
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 3 cut(s) 129, 257, 369
BstZI CGGCCG 1 cut(s) 109
BsuRI GGCC 2 cut(s) 111, 219
BtgZI GCGATG 1 cut(s) 78
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
BtuMI TCGCGA 1 cut(s) 152
CfoI GCGC 2 cut(s) 128, 147
Cfr13I GGNCC 1 cut(s) 96
Csp6I GTAC 3 cut(s) 47, 265, 351
CviAII CATG 3 cut(s) 304, 354, 395
CviJI RGCY 4 cut(s) 111, 142, 219, 360
CviKI_1 RGCY 4 cut(s) 111, 142, 219, 360
CviQI GTAC 3 cut(s) 47, 265, 351
DdeI CTNAG 1 cut(s) 313
DpnI GATC 2 cut(s) 59, 254
DpnII GATC 2 cut(s) 57, 252
EaeI YGGCCR 1 cut(s) 109
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 84, 243
EarI CTCTTC 2 cut(s) 84, 243
EclXI CGGCCG 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 96
Eco47III AGCGCT 1 cut(s) 127
Eco52I CGGCCG 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 150, 267
FaeI CATG 3 cut(s) 307, 357, 398
FaiI YATR 3 cut(s) 305, 355, 396
FatI CATG 3 cut(s) 303, 353, 394
FblI GTMKAC 2 cut(s) 117, 388
Fnu4HI GCNGC 3 cut(s) 143, 271, 358
Fsp4HI GCNGC 3 cut(s) 143, 271, 358
GlaI GCGC 2 cut(s) 127, 146
GluI GCNGC 3 cut(s) 143, 271, 358
GsuI CTGGAG 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 2 cut(s) 111, 219
HhaI GCGC 2 cut(s) 128, 147
Hin1II CATG 3 cut(s) 307, 357, 398
Hin6I GCGC 2 cut(s) 126, 145
HinP1I GCGC 2 cut(s) 126, 145
HinfI GANTC 2 cut(s) 21, 290
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 4 cut(s) 96, 106, 118, 389
Hpy188I TCNGA 3 cut(s) 289, 316, 335
Hpy188III TCNNGA 1 cut(s) 151
Hpy8I GTNNAC 4 cut(s) 96, 106, 118, 389
Hpy99I CGWCG 1 cut(s) 218
HpyCH4III ACNGT 3 cut(s) 37, 214, 350
HpyCH4V TGCA 5 cut(s) 68, 106, 273, 283, 357
HpyF3I CTNAG 1 cut(s) 313
Hsp92II CATG 3 cut(s) 307, 357, 398
HspAI GCGC 2 cut(s) 126, 145
Kzo9I GATC 2 cut(s) 57, 252
LpnPI CCDG 1 cut(s) 136
Lsp1109I GCAGC 3 cut(s) 129, 257, 369
LweI GCATC 1 cut(s) 55
MalI GATC 2 cut(s) 59, 254
MboI GATC 2 cut(s) 57, 252
MboII GAAGA 3 cut(s) 101, 143, 260
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 3 cut(s) 13, 173, 185
MmeI TCCRAC 2 cut(s) 313, 344
MnlI CCTC 2 cut(s) 158, 230
MseI TTAA 3 cut(s) 9, 189, 380
MslI CAYNNNNRTG 2 cut(s) 111, 302
MspA1I CMGCKG 1 cut(s) 360
MspCI CTTAAG 1 cut(s) 379
MvnI CGCG 1 cut(s) 152
NdeII GATC 2 cut(s) 57, 252
NlaIII CATG 3 cut(s) 307, 357, 398
NruI TCGCGA 1 cut(s) 152
NspI RCATGY 2 cut(s) 357, 398
OliI CACNNNNGTG 1 cut(s) 111
PciI ACATGT 1 cut(s) 394
PfeI GAWTC 2 cut(s) 21, 290
PkrI GCNGC 3 cut(s) 144, 272, 359
PscI ACATGT 1 cut(s) 394
PspPI GGNCC 1 cut(s) 96
PstI CTGCAG 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 360
RruI TCGCGA 1 cut(s) 152
RsaI GTAC 3 cut(s) 48, 266, 352
RsaNI GTAC 3 cut(s) 47, 265, 351
RseI CAYNNNNRTG 2 cut(s) 111, 302
SaqAI TTAA 3 cut(s) 9, 189, 380
SatI GCNGC 3 cut(s) 143, 271, 358
Sau3AI GATC 2 cut(s) 57, 252
Sau96I GGNCC 1 cut(s) 96
SduI GDGCHC 1 cut(s) 108
SetI ASST 4 cut(s) 144, 208, 362, 380
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 2 cut(s) 33, 271
SinI GGWCC 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 111, 302
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 3 cut(s) 13, 173, 185
SsiI CCGC 1 cut(s) 99
TaaI ACNGT 3 cut(s) 37, 214, 350
TaqI TCGA 1 cut(s) 293
TasI AATT 3 cut(s) 13, 173, 185
TatI WGTACW 1 cut(s) 350
TfiI GAWTC 2 cut(s) 21, 290
Tru1I TTAA 3 cut(s) 9, 189, 380
Tru9I TTAA 3 cut(s) 9, 189, 380
TscAI CASTG 1 cut(s) 108
TseI GCWGC 3 cut(s) 142, 270, 357
TspRI CASTG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 379
VneI GTGCAC 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 96
XapI RAATTY 2 cut(s) 173, 185
XceI RCATGY 2 cut(s) 357, 398
XmiI GTMKAC 2 cut(s) 117, 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.