MD12G1005400.v1.1

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003280
Physical Location & Seq
Forward (+)
665968 .. 666563
596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1005400.v1.1.491

Sequence Viewer

Length: 276 bp
ATGACAATCTCAGACAATGCTGTTGCTGTCGAAAACATCGACAACAGTACATGCAGCAGCATCGATTGGGTCGAATATGGAGTAGTCACCCCAGTAAGGAATCAGAAAAAATGTGCATGTTGTTGGGCGTTTGCCGTAGTAACCGGAATAGAAGGAATTTATGGAACACAAATGGGGATGGACAAGATGGTGCCATTGTCCCCGCACCAGCTTGTGGATTGTGACGAGTTTAGTAATGGCTGCAAGGGTGGCCTCGAGGATTACGCATATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

92

Amino Acids

9.92

Weight (kDa)

4.27

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 19 - 91 6.6e-21 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 254
AccB1I GGYRCC 1 cut(s) 190
AccB7I CCANNNNNTGG 1 cut(s) 214
AciI CCGC 1 cut(s) 203
AcsI RAATTY 1 cut(s) 156
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 2 cut(s) 96, 214
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 3 cut(s) 54, 57, 240
ApoI RAATTY 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 254
BanI GGYRCC 1 cut(s) 190
BbvI GCAGC 3 cut(s) 66, 69, 227
BccI CCATC 2 cut(s) 172, 181
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BisI GCNGC 3 cut(s) 55, 58, 241
BlsI GCNGC 3 cut(s) 56, 59, 242
BmeT110I CYCGRG 1 cut(s) 254
BmiI GGNNCC 1 cut(s) 192
BmrI ACTGGG 1 cut(s) 86
BmsI GCATC 1 cut(s) 69
BmuI ACTGGG 1 cut(s) 86
Bsa29I ATCGAT 1 cut(s) 63
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 214
Bse1I ACTGG 1 cut(s) 92
BseCI ATCGAT 1 cut(s) 63
BseGI GGATG 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 96, 214
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 92
BseXI GCAGC 3 cut(s) 66, 69, 227
BshFI GGCC 1 cut(s) 252
BshNI GGYRCC 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 96, 214
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 254
BspACI CCGC 1 cut(s) 203
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 1 cut(s) 23
BspDI ATCGAT 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 190
BsrI ACTGG 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 47
BstDEI CTNAG 1 cut(s) 10
BstF5I GGATG 1 cut(s) 183
BstMWI GCNNNNNNNGC 1 cut(s) 249
BstNSI RCATGY 2 cut(s) 54, 120
BstV1I GCAGC 3 cut(s) 66, 69, 227
Bsu15I ATCGAT 1 cut(s) 63
BsuRI GGCC 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 63
BtsCI GGATG 1 cut(s) 183
ClaI ATCGAT 1 cut(s) 63
Csp6I GTAC 1 cut(s) 48
CviAII CATG 2 cut(s) 51, 117
CviJI RGCY 3 cut(s) 211, 240, 252
CviKI_1 RGCY 3 cut(s) 211, 240, 252
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 254
FaeI CATG 2 cut(s) 54, 120
FaiI YATR 6 cut(s) 52, 78, 118, 162, 268, 270
FaqI GGGAC 1 cut(s) 184
FatI CATG 2 cut(s) 50, 116
FauI CCCGC 1 cut(s) 210
FauNDI CATATG 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 55, 58, 241
FokI GGATG 1 cut(s) 190
Fsp4HI GCNGC 3 cut(s) 55, 58, 241
GluI GCNGC 3 cut(s) 55, 58, 241
HaeIII GGCC 1 cut(s) 252
HapII CCGG 1 cut(s) 144
Hin1II CATG 2 cut(s) 54, 120
HinfI GANTC 1 cut(s) 100
HpaII CCGG 1 cut(s) 144
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 13, 105
HpyAV CCTTC 1 cut(s) 146
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4V TGCA 3 cut(s) 54, 116, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 249
HpyF3I CTNAG 1 cut(s) 10
Hsp92II CATG 2 cut(s) 54, 120
LpnPI CCDG 3 cut(s) 105, 157, 221
Lsp1109I GCAGC 3 cut(s) 66, 69, 227
LweI GCATC 1 cut(s) 69
MaeIII GTNAC 3 cut(s) 85, 139, 221
MluCI AATT 1 cut(s) 156
MnlI CCTC 2 cut(s) 250, 263
MspI CCGG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 249
NdeI CATATG 1 cut(s) 268
NlaIII CATG 2 cut(s) 54, 120
NlaIV GGNNCC 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 85, 221
NspI RCATGY 2 cut(s) 54, 120
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 69
PfeI GAWTC 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 3 cut(s) 56, 59, 242
PspN4I GGNNCC 1 cut(s) 192
PspXI VCTCGAGB 1 cut(s) 254
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SatI GCNGC 3 cut(s) 55, 58, 241
SetI ASST 1 cut(s) 213
SfaNI GCATC 1 cut(s) 69
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 156
SsiI CCGC 1 cut(s) 203
TaaI ACNGT 1 cut(s) 47
TaqI TCGA 5 cut(s) 30, 39, 63, 72, 255
TasI AATT 1 cut(s) 156
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 1 cut(s) 100
TseFI GTSAC 2 cut(s) 85, 221
TseI GCWGC 3 cut(s) 54, 57, 240
Tsp45I GTSAC 2 cut(s) 85, 221
Van91I CCANNNNNTGG 1 cut(s) 214
XapI RAATTY 1 cut(s) 156
XceI RCATGY 2 cut(s) 54, 120
XhoI CTCGAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.