Rmu_ssc0000134.1_g000025

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000134.1
OG0003280
Physical Location & Seq
Reverse (-)
85199 .. 85720
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000134.1_g000025.1.cds

Sequence Viewer

Length: 522 bp
atgagatttgagaaaagagagtttttcttattagtaatactagtgttgctcaacaattcggcaacatcagcagccacccgtccactttcagccaattctgatgatctcatcaaagtgtttgaggattgggccctcgaacacaaacgcgtctatcctactgaagaagagaaacaaaggcgatttgaaaacttcatagcaacgtatgagttcgtggacaaattgtacaaggaaggtggtgagagtaaactctacacggtcggcctgaacgcatttgctaacatgacaaatgaagaattctacaatgctcatgcttgctttatagatcatgcctccgacgtcgtctcaagctcgaccaccacctcgtcatcatcctctgctcaagtttatgaaaacaacgagcaagaattatgtggcggagtgactgactgggccgctttgagaatgattagcgctcatacatctctctttcactttgccgatctaattgttaaggtcaatttgttcatatgtccatacagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

173

Amino Acids

19.75

Weight (kDa)

5.1

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 339
AccII CGCG 1 cut(s) 147
AciI CCGC 2 cut(s) 414, 432
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 180
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 1 cut(s) 224
AfeI AGCGCT 1 cut(s) 451
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 1 cut(s) 185
AhlI ACTAGT 1 cut(s) 40
AluBI AGCT 1 cut(s) 348
AluI AGCT 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 346
Aor51HI AGCGCT 1 cut(s) 451
AoxI GGCC 3 cut(s) 129, 259, 429
ApaI GGGCCC 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 71
ApoI RAATTY 1 cut(s) 293
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 3 cut(s) 129, 130, 429
AsuHPI GGTGA 1 cut(s) 248
BaeGI GKGCMC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 346
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 1 cut(s) 41
BfoI RGCGCY 1 cut(s) 453
BisI GCNGC 2 cut(s) 72, 432
BlsI GCNGC 2 cut(s) 73, 433
BmgT120I GGNCC 3 cut(s) 129, 130, 429
BmiI GGNNCC 1 cut(s) 131
BmrI ACTGGG 1 cut(s) 436
BmuI ACTGGG 1 cut(s) 436
BpuEI CTTGAG 2 cut(s) 328, 363
BsaHI GRCGYC 1 cut(s) 336
Bse1I ACTGG 1 cut(s) 431
BseGI GGATG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 431
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 3 cut(s) 131, 261, 431
BsiEI CGRYCG 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 346
BsmBI CGTCTC 1 cut(s) 346
BsnI GGCC 3 cut(s) 131, 261, 431
Bsp120I GGGCCC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 3 cut(s) 103, 322, 478
BspACI CCGC 2 cut(s) 414, 432
BspANI GGCC 3 cut(s) 131, 261, 431
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 1 cut(s) 431
BssMI GATC 3 cut(s) 103, 322, 478
BssNI GRCGYC 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 336
BstAUI TGTACA 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 313
BstF5I GGATG 1 cut(s) 368
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 3 cut(s) 106, 325, 481
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 3 cut(s) 103, 322, 478
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstSLI GKGCMC 1 cut(s) 133
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 83
BsuRI GGCC 3 cut(s) 131, 261, 431
BtsCI GGATG 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 313
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 3 cut(s) 129, 130, 429
CseI GACGC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 223
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 3 cut(s) 280, 308, 326
CviJI RGCY 6 cut(s) 74, 92, 131, 261, 348, 431
CviKI_1 RGCY 6 cut(s) 74, 92, 131, 261, 348, 431
CviQI GTAC 1 cut(s) 223
DpnI GATC 3 cut(s) 105, 324, 480
DpnII GATC 3 cut(s) 103, 322, 478
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 133
Eco47III AGCGCT 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 133
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 3 cut(s) 283, 311, 329
FatI CATG 3 cut(s) 279, 307, 325
FauNDI CATATG 1 cut(s) 506
Fnu4HI GCNGC 2 cut(s) 72, 432
FokI GGATG 1 cut(s) 355
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 2 cut(s) 72, 432
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 451
GluI GCNGC 2 cut(s) 72, 432
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 3 cut(s) 131, 261, 431
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 3 cut(s) 283, 311, 329
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 3 cut(s) 83, 214, 245
Hpy188I TCNGA 2 cut(s) 100, 334
Hpy8I GTNNAC 3 cut(s) 83, 214, 245
Hpy99I CGWCG 2 cut(s) 338, 341
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 2 cut(s) 200, 336
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpySE526I ACGT 2 cut(s) 200, 336
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 3 cut(s) 283, 311, 329
HspAI GCGC 1 cut(s) 450
Kzo9I GATC 3 cut(s) 103, 322, 478
LpnPI CCDG 2 cut(s) 275, 412
Lsp1109I GCAGC 1 cut(s) 83
MaeI CTAG 1 cut(s) 41
MaeII ACGT 2 cut(s) 200, 336
MaeIII GTNAC 1 cut(s) 418
MalI GATC 3 cut(s) 105, 324, 480
MboI GATC 3 cut(s) 103, 322, 478
MboII GAAGA 3 cut(s) 173, 176, 302
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 7 cut(s) 55, 94, 218, 293, 404, 483, 496
MluI ACGCGT 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 5 cut(s) 115, 143, 340, 370, 382
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 113
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeI CATATG 1 cut(s) 506
NdeII GATC 3 cut(s) 103, 322, 478
NlaIII CATG 3 cut(s) 283, 311, 329
NlaIV GGNNCC 1 cut(s) 131
NmuCI GTSAC 1 cut(s) 418
PflFI GACNNNGTC 1 cut(s) 338
PkrI GCNGC 2 cut(s) 73, 433
PspN4I GGNNCC 1 cut(s) 131
PspOMI GGGCCC 1 cut(s) 129
PspPI GGNCC 3 cut(s) 129, 130, 429
PsyI GACNNNGTC 1 cut(s) 338
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 2 cut(s) 72, 432
Sau3AI GATC 3 cut(s) 103, 322, 478
Sau96I GGNCC 3 cut(s) 129, 130, 429
SduI GDGCHC 1 cut(s) 133
SetI ASST 6 cut(s) 203, 235, 339, 350, 362, 495
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 2 cut(s) 343, 378
SmoI CTYRAG 2 cut(s) 343, 378
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 7 cut(s) 55, 94, 218, 293, 404, 483, 496
SsiI CCGC 2 cut(s) 414, 432
SspMI CTAG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 2 cut(s) 203, 339
TaqI TCGA 2 cut(s) 135, 350
TasI AATT 7 cut(s) 55, 94, 218, 293, 404, 483, 496
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 434
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 4 cut(s) 181, 303, 402, 493
Tth111I GACNNNGTC 1 cut(s) 338
XapI RAATTY 1 cut(s) 293
XspI CTAG 1 cut(s) 41
ZraI GACGTC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.