Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002425

Physical Location & Seq

Genomic Coordinates Reverse (-)

27341317 .. 27345492

Gene Structure 4176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1169800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3369 bp

ATGTCTCCTGAATTGACAACAAAAGTTCGACTGGTTCGATGTCCGAAATGCCGGCTGCTTCTTCCGGAGTTGCCCGAAATTCCGGTCTACAAGTGTGGCGGATGTGGCGTCACTCTCCAAGTGAAAAATCGAATGAATGGATTGAGAAGCAAGAGAGCCAGCGTAAATGACACTGAGGCAGCTCAGAGGATTCGTTTGGATCACGGTTCGGAAGATAAAAAATCCAGAAGCTCAGGTCGTAATGCAACTCTTCCTGAATCTGGAGAATGCTCTTCAGAAGGCAACGAGTCCAGCACCTCAAGTACTCCTAAGGCTGCTTATCCTGAACCAGGAAATTGCTTTTCGAAACAAGACAATGAGAAGAATGAAGACAAGCCTTCCGAAGAACAGAAATCTAGCATCTCAAGTCATAGTGCAGCTCTTCCATACCAAAACAATGAGAGGGATGAGAGTAAATCTTCAGAAGGCAACGAATCTATCAGCTCAAGTACTCATACAGTTACTTTTGTTGACTCGGAAAAAAGCTTTCCGGACCAAAACAACGAGAAGAGAAAAGATGCGGCTTTCGAGGACAAAGAGTCTAGCATCTCAACTCATAATGCAAGTCCTTCGAACCCAGGAGAGTCCCTTTCAGACCAAAACAATGAGAAGAGTGAAGATAAGTCTTCCGAAGACAAAGAATCGAGCTCTTCAAGTAATCATGCAAGTCTTTTGATGTCAGGAGAATCCTTTTCAGACCAAAACAATGAGAGGGGCCAGAGTAAATCTTCAGAAGGTAATGAATCTAGCAGCCTAAGTCCTAAGGCTACTTTTCCGGGAGAAAGTTTTGCGGACAACCAAAACAATGAGAAGGATCAGATTGAGTCTTCAGAAGGCAACAGATTTAGCAGCTCAAGTCCAAAAGCTACTTATCCTGACTCGGGAGAATGTTTACCGGACCAAAACAACGAGAGTGATATGATTGAGTCTTCAGAAGGCAACAAGTTTAGCAGCTCAAGTCCTGAGGTAACTTTTCCTGACTCGGGAAAATGCTTCTCAGAACAAGACAATAGCAAGAGTGAAATCAAGTCTTCTGAAGTTAAGGAGACATTACCAAAAACACGAAAAGGTGCAGAAGTATCAACAAATGATGGGAGCTCACGTTTAGGAGAAACAAGTGTGGACGTAGACATCAACAAGGAGATTGATTCTGATTTCCAGAACTCAAATACTGTAAATCCAGGAGATGCAAGGGGCAGTAGTTCAATTGTTACTGCTCATACAGCCGTAAGGGGAAGCATTTCATTGGATTCCTTTAAATCTTCTCCTCATGGACGACAGGAGGAACTTCAGAATAGTGTTCCCAATGGCTTTGATCATGTAAGGTCTCCTGATGCATTTGAAAATATAGTTACTGCTCATATGCCGGAAAGGGAAAGCGTTTCATTGGATTCCCTGACATCTTCTCCTAATGGACAACAGGTGGAACCTCAGAACAGTGTTCCCATTGTCTTTGATCATGTAAGGTCTCCTGATGCATTTGAAAATAGAGTTACTGTTCATATGCCGGCAAGGGAAAGCATTTCATCGGATTCCCTGACATCTTCTCCTGATGGACAACTGGAGGAACCTCAGAATAATGCTTTTTCCAATGGCTTTGATCATGTAAGGTCTCCTGATGTATTTGAAAATACGGAGTTCTTCCCCAGCTTTGAGCTTAGCGGTGCACCTACAGATCCGTCAAAGTCCCCAGCAAATACAAGTCATCATGCATATGATGCCAGTGTTTCTTCTTATGATGGCATGGATGATCAGTTCTTCAACCGAACTACACGTTCAAATATTGTTCATTCTGAAGAACGAACCAGACGGGATAAATTCATGGCAAGTAGCATGATAAATAGAGATTCCGGATTGCAACATCAACCAACGGATCCCTGGTCAAATTTGCCCATGAAGAACGATCGTGCCATGAGATACAGAAAGTGGGATCAAGATGCATCACTGCCACCTAGAAGACAAGGTCATCCAAGCAGAGATTGGAACAGATTGCAAAGTGATGAATCTATGTATAGGATGGCTTTCCCTCAAAGAGTTTCCCAAGGTGGATACAAAAATGGAGGCCCTACAAGTCAACTGCATAACGAGTACCAGCACAATTCAGGCTACCAATCATCTGAAATGTCTGTGGAGGCTGAACAGGACAAGATGACACTATTGAGAATGGTTTATGAACTGCAGAATCAAGTTAACAGTTTGAATGGAAAGGCGAGTGGAAGGGTTGCCGGAGGAGCCACTTGGAATGAGAATAGCATGCCCCAGTACCGTAGCTATGAGGCATTCGAGAAGGAACTCTATCATGATCCAAACTATCAGAGGTATCTGAGAAGACACAGGACAGGCAGCCTCTATCCCCCCCAGCACCACCGCAAAAACATGCGCATACCTTTCTCGAGTGAGGCAACAACCAGCAGGCACCAACCTGATTCTTCATATTTGCATTATGGCCCCCAAGACTGGAAGTGCTCAGCACCATTGTCTCAACCCGTTCGTTGCAGCAAAAACGGGTTATGTAGGGTTCACCCTGGTCACAGTTGCTGGACATCCTATGATAAGTCTCGTCCTTCAACTCCCGAACGGTATATGGGATCCGATTCTCCTCTTTGGGGCCATGAAACAATGTCTGATGATCTAAGGCACCAATGGCATGATGCGAAGAAGTTTTACAGAGAGAAACAACATTTGGCTAAACGACATTTCCGCCCCATAGCAGGTGGAGCCCCTTTCATAACTTGTTACAACTGCTCAGAACTGCTGCAGATACCTGCAGATTTTCTCCTTTTCAAGAGGAGATACCATAGACTTCGATGTGGGGCTTGTTCGGAGGTGCTCAAGTTTTCGCTCCAAAAGAGAGCTCATATTGTCCCCTATGAACAGAATGCTATAGCCCCTCCGAGTGAGGTTGGCGACTATAATGCTGCCGGTAATGGCGGTAACTTGGCTCACCATGCTGATCCTGTTTCATGCTCTGATGATTATGGTTATGGACCCTCTTACTACCATAGTTACTCTTCTGACGGTGATCCTGGAGCTTTTGCACCCTCCAACTCTAACCATGGTGATTCAGAGGACCAAAATATGTCACATAATTCATTGGATCCAATGAGAGAGAGGAAGAAGCTTGTTTTGAGAGAGTCTCAGAACAAAGAAAAGAATCCAATTGAAACAATTGTGTCAGCAAGACCGTCGTTATCCTCAGAAATCGAAGAGCTGCCAGCAAATTCGAGTTCACCACTTCATCGACTCATGGGCTACGCTTCGCCAAGCCTAGTGATTAGAGGGTCTGTAACATCTCGGACAGGGAGAAGCTCATACCCATTGCAGAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zinc_ribbon_12

Protein Analysis

1123

Amino Acids

124.86

Weight (kDa)

5.8

Isoelectric Point (pI)

66.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EDR4-like_1st	PF22910	8 - 40	2.4e-17	Enhanced disease resistance 4-like, N-terminal domain
Zn_ribbon_12	PF11331	917 - 961	3e-23	EDR4 zinc-ribbon domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05190
fragaria_vesca	FvH4_7g26160
malus_domestica	MD01G1169800.v1.1 MD01G1169900.v1.1 MD07G1235500.v1.1
prunus_persica	Prupe.2G265800_v2.0.a1 Prupe.2G265800_v2.0.a1 Prupe.2G265800_v2.0.a1 Prupe.4G213000_v2.0.a1 Prupe.4G213000_v2.0.a1 Prupe.4G213000_v2.0.a1 Prupe.4G213000_v2.0.a1 Prupe.4G213000_v2.0.a1
pyrus_communis	pycom01g18250 pycom01g18260 pycom07g21310
rosa_chinensis	RchiOBHm_Chr1g0372591 RchiOBHm_Chr4g0399021 RchiOBHm_Chr5g0049731 RchiOBHm_Chr7g0243711
rosa_laevigata	RLG00000001030 RLG00000008808 RLG00000010509 RLG00000026888
rosa_multiflora	Rmu_ssc0000419.1_g000010
rosa_roxburghii	Rroxscaffold_7G00208520
rosa_rugosa	Rorug01G0066000 Rorug01G0373300
rosa_samantha	Rh1AG381600 Rh1BG345000 Rh1CG358400 Rh1DG376200 Rh2BG091800 Rh3DG186100 Rh4DG129900 Rh5AG465400 Rh7AG476000
rosa_wichuraiana	Rw0G018150 Rw1G033560 Rw2G022780 Rw7G041610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2741
Acc16I	TGCGCA	1 cut(s)	2420
Acc36I	ACCTGC	2 cut(s)	2741, 2812
AccB1I	GGYRCC	2 cut(s)	2454, 2676
AccI	GTMKAC	2 cut(s)	87, 1167
AccIII	TCCGGA	3 cut(s)	64, 529, 1889
AciI	CCGC	7 cut(s)	99, 560, 830, 1701, 2407, 2740, 2970
AcsI	RAATTY	4 cut(s)	78, 1856, 1924, 3259
AcuI	CTGAAG	8 cut(s)	258, 444, 753, 852, 954, 1095, 1313, 1854
AcyI	GRCGYC	1 cut(s)	108
AfaI	GTAC	4 cut(s)	304, 490, 2129, 2303
AfiI	CCNNNNNNNGG	7 cut(s)	260, 329, 920, 1022, 2496, 2645, 2750
AflIII	ACRYGT	1 cut(s)	1811
AhdI	GACNNNNNGTC	1 cut(s)	577
AjnI	CCWGG	6 cut(s)	328, 616, 1219, 1916, 2563, 3064
AjuI	GAANNNNNNNTTGG	2 cut(s)	2705, 2737
AloI	GAACNNNNNNTCC	2 cut(s)	1778, 1810
Alw21I	GWGCWC	6 cut(s)	689, 1139, 1708, 2507, 2871, 2896
Alw26I	GTCTC	8 cut(s)	9, 1079, 1371, 1512, 1656, 2523, 2601, 3180
Alw44I	GTGCAC	1 cut(s)	1704
AlwNI	CAGNNNCTG	1 cut(s)	2577
Ama87I	CYCGRG	3 cut(s)	919, 1021, 2431
Aor13HI	TCCGGA	3 cut(s)	64, 529, 1889
AoxI	GGCC	4 cut(s)	754, 2101, 2485, 2647
ApaLI	GTGCAC	1 cut(s)	1704
ApoI	RAATTY	4 cut(s)	78, 1856, 1924, 3259
ArsI	GACNNNNNNTTYG	2 cut(s)	69, 101
Asp700I	GAANNNNTTC	1 cut(s)	1279
AspLEI	GCGC	1 cut(s)	2421
AspS9I	GGNCC	8 cut(s)	532, 754, 937, 2102, 2486, 2647, 3026, 3109
AsuC2I	CCSGG	1 cut(s)	816
AsuHPI	GGTGA	5 cut(s)	2552, 2975, 3071, 3110, 3261
AsuII	TTCGAA	2 cut(s)	344, 611
AvaI	CYCGRG	3 cut(s)	919, 1021, 2431
AvaII	GGWCC	4 cut(s)	532, 937, 3026, 3109
AxyI	CCTNAGG	3 cut(s)	309, 801, 1002
BaeGI	GKGCMC	1 cut(s)	1708
BamHI	GGATCC	3 cut(s)	1912, 2627, 3136
BanI	GGYRCC	2 cut(s)	2454, 2676
BanII	GRGCYC	4 cut(s)	689, 1139, 2761, 2896
BarI	GAAGNNNNNNTAC	2 cut(s)	2687, 2719
BbsI	GAAGAC	8 cut(s)	375, 657, 678, 858, 960, 1062, 2002, 2374
Bbv12I	GWGCWC	6 cut(s)	689, 1139, 1708, 2507, 2871, 2896
BccI	CCATC	4 cut(s)	1124, 1586, 1772, 2050
BceAI	ACGGC	1 cut(s)	1250
BcgI	CGANNNNNNTGC	6 cut(s)	591, 625, 2936, 2970, 3207, 3241
BciT130I	CCWGG	6 cut(s)	330, 618, 1221, 1918, 2565, 3066
BciVI	GTATCC	1 cut(s)	2081
BclI	TGATCA	4 cut(s)	1354, 1495, 1639, 1789
BcnI	CCSGG	1 cut(s)	816
BcoDI	GTCTC	8 cut(s)	9, 1079, 1371, 1512, 1656, 2523, 2601, 3180
BfaI	CTAG	5 cut(s)	396, 582, 786, 1992, 3308
BfmI	CTRYAG	5 cut(s)	1710, 2216, 2795, 2805, 2922
BfuAI	ACCTGC	2 cut(s)	2741, 2812
BfuI	GTATCC	1 cut(s)	2081
BlpI	GCTNAGC	2 cut(s)	1697, 2506
BmcAI	AGTACT	2 cut(s)	304, 490
Bme1390I	CCNGG	7 cut(s)	330, 618, 816, 1221, 1918, 2565, 3066
Bme18I	GGWCC	4 cut(s)	532, 937, 3026, 3109
BmeRI	GACNNNNNGTC	1 cut(s)	577
BmeT110I	CYCGRG	3 cut(s)	919, 1021, 2431
BmgT120I	GGNCC	8 cut(s)	532, 754, 937, 2102, 2486, 2647, 3026, 3109
BmrFI	CCNGG	7 cut(s)	330, 618, 816, 1221, 1918, 2565, 3066
BmrI	ACTGGG	1 cut(s)	2293
BmuI	ACTGGG	1 cut(s)	2293
BpiI	GAAGAC	8 cut(s)	375, 657, 678, 858, 960, 1062, 2002, 2374
BplI	GAGNNNNNCTC	2 cut(s)	3160, 3192
BpmI	CTGGAG	3 cut(s)	282, 1622, 3087
Bpu10I	CCTNAGC	1 cut(s)	232
Bpu1102I	GCTNAGC	2 cut(s)	1697, 2506
Bpu14I	TTCGAA	2 cut(s)	344, 611
BpuEI	CTTGAG	6 cut(s)	283, 388, 469, 877, 979, 2855
BpuMI	CCSGG	1 cut(s)	816
BsaHI	GRCGYC	1 cut(s)	108
BsaI	GGTCTC	3 cut(s)	1371, 1512, 1656
BsaJI	CCNNGG	5 cut(s)	616, 1916, 2080, 2563, 3094
BsaWI	WCCGGW	5 cut(s)	64, 82, 529, 934, 1889
BsaXI	ACNNNNNCTCC	6 cut(s)	1126, 1156, 1429, 1459, 1570, 1600
Bsc4I	CCNNNNNNNGG	7 cut(s)	260, 329, 920, 1022, 2496, 2645, 2750
Bse118I	RCCGGY	3 cut(s)	51, 1546, 2960
Bse1I	ACTGG	5 cut(s)	36, 1605, 1761, 2299, 2501
Bse21I	CCTNAGG	3 cut(s)	309, 801, 1002
Bse3DI	GCAATG	1 cut(s)	3356
BseAI	TCCGGA	3 cut(s)	64, 529, 1889
BseBI	CCWGG	6 cut(s)	330, 618, 1221, 1918, 2565, 3066
BseDI	CCNNGG	5 cut(s)	616, 1916, 2080, 2563, 3094
BseGI	GGATG	6 cut(s)	107, 451, 1792, 2005, 2061, 2582
BseLI	CCNNNNNNNGG	7 cut(s)	260, 329, 920, 1022, 2496, 2645, 2750
BseMI	GCAATG	1 cut(s)	3356
BseNI	ACTGG	5 cut(s)	36, 1605, 1761, 2299, 2501
BseRI	GAGGAG	4 cut(s)	1296, 2283, 2628, 2842
BseSI	GKGCMC	1 cut(s)	1708
BseYI	CCCAGC	3 cut(s)	1685, 1729, 2397
BsgI	GTGCAG	2 cut(s)	435, 1131
Bsh1285I	CGRYCG	1 cut(s)	1945
BshFI	GGCC	4 cut(s)	756, 2103, 2487, 2649
BshNI	GGYRCC	2 cut(s)	2454, 2676
BsiEI	CGRYCG	1 cut(s)	1945
BsiHKAI	GWGCWC	6 cut(s)	689, 1139, 1708, 2507, 2871, 2896
BsiHKCI	CYCGRG	3 cut(s)	919, 1021, 2431
BslFI	GGGAC	3 cut(s)	610, 1711, 2888
BslI	CCNNNNNNNGG	7 cut(s)	260, 329, 920, 1022, 2496, 2645, 2750
BsmAI	GTCTC	8 cut(s)	9, 1079, 1371, 1512, 1656, 2523, 2601, 3180
BsmFI	GGGAC	3 cut(s)	610, 1711, 2888
BsmI	GAATGC	3 cut(s)	272, 2318, 2923
BsnI	GGCC	4 cut(s)	756, 2103, 2487, 2649
Bso31I	GGTCTC	3 cut(s)	1371, 1512, 1656
BsoBI	CYCGRG	3 cut(s)	919, 1021, 2431
Bsp119I	TTCGAA	2 cut(s)	344, 611
Bsp1286I	GDGCHC	7 cut(s)	689, 1139, 1708, 2507, 2761, 2871, 2896
Bsp13I	TCCGGA	3 cut(s)	64, 529, 1889
Bsp1720I	GCTNAGC	2 cut(s)	1697, 2506
Bsp19I	CCATGG	1 cut(s)	3094
BspACI	CCGC	7 cut(s)	99, 560, 830, 1701, 2407, 2740, 2970
BspANI	GGCC	4 cut(s)	756, 2103, 2487, 2649
BspEI	TCCGGA	3 cut(s)	64, 529, 1889
BspHI	TCATGA	1 cut(s)	2338
BspMAI	CTGCAG	3 cut(s)	2220, 2799, 2809
BspMI	ACCTGC	2 cut(s)	2741, 2812
BspQI	GCTCTTC	4 cut(s)	277, 426, 694, 3240
BspT104I	TTCGAA	2 cut(s)	344, 611
BspT107I	GGYRCC	2 cut(s)	2454, 2676
BspTNI	GGTCTC	3 cut(s)	1371, 1512, 1656
BsrDI	GCAATG	1 cut(s)	3356
BsrFI	RCCGGY	3 cut(s)	51, 1546, 2960
BsrI	ACTGG	5 cut(s)	36, 1605, 1761, 2299, 2501
BssAI	RCCGGY	3 cut(s)	51, 1546, 2960
BssECI	CCNNGG	5 cut(s)	616, 1916, 2080, 2563, 3094
BssNI	GRCGYC	1 cut(s)	108
BssT1I	CCWWGG	2 cut(s)	2080, 3094
Bst2UI	CCWGG	6 cut(s)	330, 618, 1221, 1918, 2565, 3066
Bst6I	CTCTTC	8 cut(s)	255, 277, 426, 542, 644, 694, 3055, 3240
BstACI	GRCGYC	1 cut(s)	108
BstAPI	GCANNNNNTGC	1 cut(s)	1757
BstBI	TTCGAA	2 cut(s)	344, 611
BstC8I	GCNNGC	6 cut(s)	53, 160, 1548, 2294, 2453, 3255
BstDSI	CCRYGG	1 cut(s)	3094
BstENI	CCTNNNNNAGG	1 cut(s)	327
BstF5I	GGATG	6 cut(s)	107, 451, 1792, 2005, 2061, 2582
BstHHI	GCGC	1 cut(s)	2421
BstMAI	GTCTC	8 cut(s)	9, 1079, 1371, 1512, 1656, 2523, 2601, 3180
BstMCI	CGRYCG	1 cut(s)	1945
BstMWI	GCNNNNNNNGC	7 cut(s)	105, 1262, 1757, 2270, 2683, 2756, 2987
BstNI	CCWGG	6 cut(s)	330, 618, 1221, 1918, 2565, 3066
BstNSI	RCATGY	2 cut(s)	2296, 2419
BstSCI	CCNGG	7 cut(s)	328, 616, 814, 1219, 1916, 2563, 3064
BstSFI	CTRYAG	5 cut(s)	1710, 2216, 2795, 2805, 2922
BstSLI	GKGCMC	1 cut(s)	1708
BstV2I	GAAGAC	8 cut(s)	375, 657, 678, 858, 960, 1062, 2002, 2374
BstX2I	RGATCY	4 cut(s)	1714, 1912, 2627, 3136
BstYI	RGATCY	4 cut(s)	1714, 1912, 2627, 3136
Bsu36I	CCTNAGG	3 cut(s)	309, 801, 1002
BsuI	GTATCC	1 cut(s)	2081
BsuRI	GGCC	4 cut(s)	756, 2103, 2487, 2649
BtgI	CCRYGG	1 cut(s)	3094
BtsCI	GGATG	6 cut(s)	107, 451, 1792, 2005, 2061, 2582
BtsI	GCAGTG	1 cut(s)	1982
BtsIMutI	CAGTG	4 cut(s)	171, 1483, 1768, 1982
BveI	ACCTGC	2 cut(s)	2741, 2812
Cac8I	GCNNGC	6 cut(s)	53, 160, 1548, 2294, 2453, 3255
CaiI	CAGNNNCTG	1 cut(s)	2577
CciI	TCATGA	1 cut(s)	2338
CfoI	GCGC	1 cut(s)	2421
Cfr10I	RCCGGY	3 cut(s)	51, 1546, 2960
Cfr13I	GGNCC	8 cut(s)	532, 754, 937, 2102, 2486, 2647, 3026, 3109
CseI	GACGC	1 cut(s)	97
Csp6I	GTAC	4 cut(s)	303, 489, 2128, 2302
CviQI	GTAC	4 cut(s)	303, 489, 2128, 2302
DraI	TTTAAA	1 cut(s)	1297
DriI	GACNNNNNGTC	1 cut(s)	577
Eam1104I	CTCTTC	8 cut(s)	255, 277, 426, 542, 644, 694, 3055, 3240
Eam1105I	GACNNNNNGTC	1 cut(s)	577
EarI	CTCTTC	8 cut(s)	255, 277, 426, 542, 644, 694, 3055, 3240
EciI	GGCGGA	2 cut(s)	114, 2729
Ecl136II	GAGCTC	3 cut(s)	687, 1137, 2894
Eco130I	CCWWGG	2 cut(s)	2080, 3094
Eco24I	GRGCYC	4 cut(s)	689, 1139, 2761, 2896
Eco31I	GGTCTC	3 cut(s)	1371, 1512, 1656
Eco47I	GGWCC	4 cut(s)	532, 937, 3026, 3109
Eco53kI	GAGCTC	3 cut(s)	687, 1137, 2894
Eco57I	CTGAAG	8 cut(s)	258, 444, 753, 852, 954, 1095, 1313, 1854
Eco81I	CCTNAGG	3 cut(s)	309, 801, 1002
Eco88I	CYCGRG	3 cut(s)	919, 1021, 2431
EcoICRI	GAGCTC	3 cut(s)	687, 1137, 2894
EcoNI	CCTNNNNNAGG	1 cut(s)	327
EcoO109I	RGGNCCY	1 cut(s)	2102
EcoRII	CCWGG	6 cut(s)	328, 616, 1219, 1916, 2563, 3064
EcoT14I	CCWWGG	2 cut(s)	2080, 3094
EcoT22I	ATGCAT	4 cut(s)	1378, 1519, 1753, 1981
EcoT38I	GRGCYC	4 cut(s)	689, 1139, 2761, 2896
ErhI	CCWWGG	2 cut(s)	2080, 3094
FaqI	GGGAC	3 cut(s)	610, 1711, 2888
FauNDI	CATATG	3 cut(s)	1401, 1542, 1753
FbaI	TGATCA	4 cut(s)	1354, 1495, 1639, 1789
FblI	GTMKAC	2 cut(s)	87, 1167
FokI	GGATG	6 cut(s)	114, 458, 1799, 1992, 2068, 2569
FriOI	GRGCYC	4 cut(s)	689, 1139, 2761, 2896
FspAI	RTGCGCAY	1 cut(s)	2420
FspBI	CTAG	5 cut(s)	396, 582, 786, 1992, 3308
FspI	TGCGCA	1 cut(s)	2420
GlaI	GCGC	1 cut(s)	2420
GsaI	CCCAGC	3 cut(s)	1689, 1733, 2401
GsuI	CTGGAG	3 cut(s)	282, 1622, 3087
HaeIII	GGCC	4 cut(s)	756, 2103, 2487, 2649
HgaI	GACGC	1 cut(s)	97
HhaI	GCGC	1 cut(s)	2421
Hin1I	GRCGYC	1 cut(s)	108
Hin6I	GCGC	1 cut(s)	2419
HinP1I	GCGC	1 cut(s)	2419
HincII	GTYRAC	3 cut(s)	511, 2114, 2230
HindII	GTYRAC	3 cut(s)	511, 2114, 2230
HindIII	AAGCTT	2 cut(s)	523, 3158
HpaI	GTTAAC	1 cut(s)	2230
HphI	GGTGA	5 cut(s)	2552, 2975, 3071, 3110, 3261
Hpy99I	CGWCG	1 cut(s)	3229
HpyCH4IV	ACGT	3 cut(s)	1141, 1164, 1813
HpyF10VI	GCNNNNNNNGC	7 cut(s)	105, 1262, 1757, 2270, 2683, 2756, 2987
HpySE526I	ACGT	3 cut(s)	1141, 1164, 1813
Hsp92I	GRCGYC	1 cut(s)	108
HspAI	GCGC	1 cut(s)	2419
Kpn2I	TCCGGA	3 cut(s)	64, 529, 1889
KroI	GCCGGC	2 cut(s)	51, 1546
KroNI	GCCGGC	2 cut(s)	53, 1548
Ksp22I	TGATCA	4 cut(s)	1354, 1495, 1639, 1789
KspAI	GTTAAC	1 cut(s)	2230
LguI	GCTCTTC	4 cut(s)	277, 426, 694, 3240
LmnI	GCTCC	5 cut(s)	1134, 2270, 2756, 2886, 3068
MaeI	CTAG	5 cut(s)	396, 582, 786, 1992, 3308
MaeII	ACGT	3 cut(s)	1141, 1164, 1813
MfeI	CAATTG	3 cut(s)	1245, 3198, 3207
MflI	RGATCY	4 cut(s)	1714, 1912, 2627, 3136
MhlI	GDGCHC	7 cut(s)	689, 1139, 1708, 2507, 2761, 2871, 2896
MmeI	TCCRAC	1 cut(s)	3108
Mph1103I	ATGCAT	4 cut(s)	1378, 1519, 1753, 1981
MroI	TCCGGA	3 cut(s)	64, 529, 1889
MroNI	GCCGGC	2 cut(s)	51, 1546
MroXI	GAANNNNTTC	1 cut(s)	1279
MseI	TTAA	3 cut(s)	1080, 1296, 2229
MslI	CAYNNNNRTG	1 cut(s)	1752
MspR9I	CCNGG	7 cut(s)	330, 618, 816, 1221, 1918, 2565, 3066
MunI	CAATTG	3 cut(s)	1245, 3198, 3207
Mva1269I	GAATGC	3 cut(s)	272, 2318, 2923
MvaI	CCWGG	6 cut(s)	330, 618, 1221, 1918, 2565, 3066
MwoI	GCNNNNNNNGC	7 cut(s)	105, 1262, 1757, 2270, 2683, 2756, 2987
NaeI	GCCGGC	2 cut(s)	53, 1548
NciI	CCSGG	1 cut(s)	816
NcoI	CCATGG	1 cut(s)	3094
NdeI	CATATG	3 cut(s)	1401, 1542, 1753
NgoMIV	GCCGGC	2 cut(s)	51, 1546
NmuCI	GTSAC	3 cut(s)	109, 2567, 3120
NsbI	TGCGCA	1 cut(s)	2420
NsiI	ATGCAT	4 cut(s)	1378, 1519, 1753, 1981
NspI	RCATGY	2 cut(s)	2296, 2419
NspV	TTCGAA	2 cut(s)	344, 611
PaeI	GCATGC	1 cut(s)	2296
PaeR7I	CTCGAG	1 cut(s)	2431
PagI	TCATGA	1 cut(s)	2338
PaqCI	CACCTGC	1 cut(s)	2741
PciSI	GCTCTTC	4 cut(s)	277, 426, 694, 3240
PcsI	WCGNNNNNNNCGW	1 cut(s)	34
PctI	GAATGC	3 cut(s)	272, 2318, 2923
PdiI	GCCGGC	2 cut(s)	53, 1548
PdmI	GAANNNNTTC	1 cut(s)	1279
PflFI	GACNNNGTC	1 cut(s)	2001
PfoI	TCCNGGA	3 cut(s)	814, 1219, 3064
Ple19I	CGATCG	1 cut(s)	1945
Psp124BI	GAGCTC	3 cut(s)	689, 1139, 2896
Psp6I	CCWGG	6 cut(s)	328, 616, 1219, 1916, 2563, 3064
PspFI	CCCAGC	3 cut(s)	1685, 1729, 2397
PspGI	CCWGG	6 cut(s)	328, 616, 1219, 1916, 2563, 3064
PspPI	GGNCC	8 cut(s)	532, 754, 937, 2102, 2486, 2647, 3026, 3109
PstI	CTGCAG	3 cut(s)	2220, 2799, 2809
PstNI	CAGNNNCTG	1 cut(s)	2577
PsuI	RGATCY	4 cut(s)	1714, 1912, 2627, 3136
PsyI	GACNNNGTC	1 cut(s)	2001
PvuI	CGATCG	1 cut(s)	1945
RsaI	GTAC	4 cut(s)	304, 490, 2129, 2303
RsaNI	GTAC	4 cut(s)	303, 489, 2128, 2302
RseI	CAYNNNNRTG	1 cut(s)	1752
SacI	GAGCTC	3 cut(s)	689, 1139, 2896
SapI	GCTCTTC	4 cut(s)	277, 426, 694, 3240
SaqAI	TTAA	3 cut(s)	1080, 1296, 2229
Sau96I	GGNCC	8 cut(s)	532, 754, 937, 2102, 2486, 2647, 3026, 3109
ScaI	AGTACT	2 cut(s)	304, 490
ScrFI	CCNGG	7 cut(s)	330, 618, 816, 1221, 1918, 2565, 3066
SduI	GDGCHC	7 cut(s)	689, 1139, 1708, 2507, 2761, 2871, 2896
SfcI	CTRYAG	5 cut(s)	1710, 2216, 2795, 2805, 2922
Sfr274I	CTCGAG	1 cut(s)	2431
SfuI	TTCGAA	2 cut(s)	344, 611
SinI	GGWCC	4 cut(s)	532, 937, 3026, 3109
SlaI	CTCGAG	1 cut(s)	2431
SmiMI	CAYNNNNRTG	1 cut(s)	1752
SmlI	CTYRAG	7 cut(s)	298, 403, 484, 892, 994, 2431, 2870
SmoI	CTYRAG	7 cut(s)	298, 403, 484, 892, 994, 2431, 2870
SphI	GCATGC	1 cut(s)	2296
SsiI	CCGC	7 cut(s)	99, 560, 830, 1701, 2407, 2740, 2970
SspI	AATATT	1 cut(s)	1822
SspMI	CTAG	5 cut(s)	396, 582, 786, 1992, 3308
SstI	GAGCTC	3 cut(s)	689, 1139, 2896
StyD4I	CCNGG	7 cut(s)	328, 616, 814, 1219, 1916, 2563, 3064
StyI	CCWWGG	2 cut(s)	2080, 3094
TaiI	ACGT	3 cut(s)	1144, 1167, 1816
TatI	WGTACW	2 cut(s)	302, 488
TauI	GCSGC	1 cut(s)	563
Tru1I	TTAA	3 cut(s)	1080, 1296, 2229
Tru9I	TTAA	3 cut(s)	1080, 1296, 2229
TscAI	CASTG	4 cut(s)	178, 1483, 1768, 1989
TseFI	GTSAC	3 cut(s)	109, 2567, 3120
Tsp45I	GTSAC	3 cut(s)	109, 2567, 3120
TspGWI	ACGGA	3 cut(s)	1688, 1707, 1925
TspRI	CASTG	4 cut(s)	178, 1483, 1768, 1989
Tth111I	GACNNNGTC	1 cut(s)	2001
VneI	GTGCAC	1 cut(s)	1704
VpaK11BI	GGWCC	4 cut(s)	532, 937, 3026, 3109
XagI	CCTNNNNNAGG	1 cut(s)	327
XapI	RAATTY	4 cut(s)	78, 1856, 1924, 3259
XceI	RCATGY	2 cut(s)	2296, 2419
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1914, 2016
XhoI	CTCGAG	1 cut(s)	2431
XmiI	GTMKAC	2 cut(s)	87, 1167
XmnI	GAANNNNTTC	1 cut(s)	1279
XspI	CTAG	5 cut(s)	396, 582, 786, 1992, 3308
ZrmI	AGTACT	2 cut(s)	304, 490
Zsp2I	ATGCAT	4 cut(s)	1378, 1519, 1753, 1981

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.