Rw2G022780

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002425
Physical Location & Seq
Forward (+)
32636245 .. 32638896
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022780.1

Sequence Viewer

Length: 399 bp
ATGGGTCACCAGGAGCAGGATGACAAGAAGGCCCCTGACTCTGCTGCTAAAGTCAAAGGCGAATCAGCCAAGAAGGGATATGTTGGAATTCACATCTCAGGATTCAGAGATATCCTTTTAAGGCTGGAGCTTGTACGCCCGATTGTGGACTCAATATTTGAGCATCCTTTTGAAGCAATGGGGGACTCAGGTGGAAAAGTGAGGTTGGTTCAGTGCCCAAAGTGTGAGAATCTTCTTCCTGAGCTAGCTGATTACTCTATGTATCAGTGTGGTGCTGTTCTTAGAGTACAAGGATTACAGATTGTGACTGGGATTACAGATGGATGGAGTACCCAGCTCATGCAAGAACTTCGAGACATCCCTTGGATCAGTCGTTGGCCACATATGGAGACCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

132

Amino Acids

14.82

Weight (kDa)

5.77

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR4-like_1st PF22910 66 - 92 9.9e-15 Enhanced disease resistance 4-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 377
AcsI RAATTY 1 cut(s) 87
AfaI GTAC 3 cut(s) 135, 288, 331
AfiI CCNNNNNNNGG 2 cut(s) 16, 145
AgsI TTSAA 1 cut(s) 173
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 4 cut(s) 130, 244, 248, 337
AluI AGCT 4 cut(s) 130, 244, 248, 337
Alw26I GTCTC 2 cut(s) 348, 383
AlwI GGATC 1 cut(s) 374
AoxI GGCC 2 cut(s) 30, 377
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 1 cut(s) 87
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
AspS9I GGNCC 1 cut(s) 31
AsuNHI GCTAGC 1 cut(s) 244
BaeGI GKGCMC 1 cut(s) 218
BalI TGGCCA 1 cut(s) 379
BbvI GCAGC 1 cut(s) 31
BccI CCATC 2 cut(s) 314, 318
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 2 cut(s) 348, 383
BfaI CTAG 1 cut(s) 245
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 172
BmtI GCTAGC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 362
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 145
Bse1I ACTGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 362
BseGI GGATG 4 cut(s) 25, 163, 329, 357
BseLI CCNNNNNNNGG 2 cut(s) 16, 145
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 3 cut(s) 111, 201, 231
BseNI ACTGG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 333
BshFI GGCC 2 cut(s) 32, 379
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 16, 145
BsmAI GTCTC 2 cut(s) 348, 383
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 2 cut(s) 32, 379
Bso31I GGTCTC 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 218
Bsp143I GATC 1 cut(s) 366
BspANI GGCC 2 cut(s) 32, 379
BspCNI CTCAG 3 cut(s) 110, 200, 232
BspLI GGNNCC 1 cut(s) 33
BspOI GCTAGC 1 cut(s) 248
BspPI GGATC 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 362
BssMI GATC 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 4 cut(s) 97, 187, 240, 281
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 4 cut(s) 25, 163, 329, 357
BstKTI GATC 1 cut(s) 369
BstMAI GTCTC 2 cut(s) 348, 383
BstMBI GATC 1 cut(s) 366
BstNI CCWGG 1 cut(s) 11
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 31
BsuRI GGCC 2 cut(s) 32, 379
BtsCI GGATG 4 cut(s) 25, 163, 329, 357
BtsIMutI CAGTG 2 cut(s) 218, 272
Cac8I GCNNGC 1 cut(s) 246
Cfr13I GGNCC 1 cut(s) 31
Csp6I GTAC 3 cut(s) 134, 287, 330
CviAII CATG 1 cut(s) 340
CviJI RGCY 8 cut(s) 32, 68, 124, 130, 244, 248, 337, 379
CviKI_1 RGCY 8 cut(s) 32, 68, 124, 130, 244, 248, 337, 379
CviQI GTAC 3 cut(s) 134, 287, 330
DdeI CTNAG 4 cut(s) 97, 187, 240, 281
DpnI GATC 1 cut(s) 368
DpnII GATC 1 cut(s) 366
EaeI YGGCCR 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 383
Eco32I GATATC 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 5
EcoO109I RGGNCCY 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 9
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 1 cut(s) 343
FaiI YATR 5 cut(s) 81, 260, 341, 384, 386
FaqI GGGAC 1 cut(s) 197
FatI CATG 1 cut(s) 339
FauNDI CATATG 1 cut(s) 384
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 4 cut(s) 32, 150, 336, 344
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 1 cut(s) 245
GluI GCNGC 1 cut(s) 45
GsaI CCCAGC 1 cut(s) 337
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 2 cut(s) 32, 379
Hin1II CATG 1 cut(s) 343
HinfI GANTC 6 cut(s) 38, 62, 102, 149, 185, 229
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 107
Hpy188III TCNNGA 3 cut(s) 99, 239, 353
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 2 cut(s) 22, 67
HpyCH4V TGCA 1 cut(s) 343
HpyF3I CTNAG 4 cut(s) 97, 187, 240, 281
Hsp92II CATG 1 cut(s) 343
Kzo9I GATC 1 cut(s) 366
LmnI GCTCC 2 cut(s) 13, 127
LpnPI CCDG 9 cut(s) 2, 23, 48, 84, 110, 174, 252, 294, 347
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 172
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 2 cut(s) 5, 304
MalI GATC 1 cut(s) 368
MboI GATC 1 cut(s) 366
MboII GAAGA 2 cut(s) 224, 227
MhlI GDGCHC 1 cut(s) 218
MlsI TGGCCA 1 cut(s) 379
MluCI AATT 1 cut(s) 87
MluNI TGGCCA 1 cut(s) 379
MlyI GAGTC 3 cut(s) 32, 143, 179
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 379
MscI TGGCCA 1 cut(s) 379
MseI TTAA 1 cut(s) 119
Msp20I TGGCCA 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
NdeI CATATG 1 cut(s) 384
NdeII GATC 1 cut(s) 366
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 2 cut(s) 5, 304
PfeI GAWTC 3 cut(s) 62, 102, 229
PkrI GCNGC 1 cut(s) 46
PleI GAGTC 3 cut(s) 32, 143, 179
PpsI GAGTC 3 cut(s) 32, 143, 179
Psp6I CCWGG 1 cut(s) 9
PspEI GGTNACC 1 cut(s) 5
PspFI CCCAGC 1 cut(s) 333
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 31
RsaI GTAC 3 cut(s) 135, 288, 331
RsaNI GTAC 3 cut(s) 134, 287, 330
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 1 cut(s) 366
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 3 cut(s) 32, 143, 179
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 218
SetI ASST 6 cut(s) 132, 193, 206, 246, 250, 339
SfaNI GCATC 1 cut(s) 172
Sse9I AATT 1 cut(s) 87
SspI AATATT 1 cut(s) 156
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 362
TaqI TCGA 1 cut(s) 352
TasI AATT 1 cut(s) 87
TatI WGTACW 1 cut(s) 286
TfiI GAWTC 3 cut(s) 62, 102, 229
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 2 cut(s) 218, 272
TseFI GTSAC 2 cut(s) 5, 304
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 2 cut(s) 5, 304
TspRI CASTG 2 cut(s) 218, 272
XapI RAATTY 1 cut(s) 87
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.