Rh5AG465400

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002425
Physical Location & Seq
Forward (+)
79771268 .. 79773928
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG465400.1

Sequence Viewer

Length: 501 bp
ATGGGTCACCAGGTGCTGCCCCGAGATTCCCAGAAGCCTTGTTCAGAAGCTCTTTCGCTTAAGACAGATTCTGTTGTTCCTTGTCTATTGAAATTCCTGGCACTGTGTTATAGAGAGCAGGATGACAAGAAGGCCCCTGACTCTGCTGCTAAAGTCAAAGGCGAATCAGCCAAGAAGGGATATGTTGGAATTCACATCTCAGGATTCAGAGATATCCTTTTAAGGCCGGAGCTTGTACGCCCTATTGTGGACTCAATATTTGAGCATCCTTTTGAAGCAATGGGGGACTCAGGTGGAAAAGTGAGGTTGGTTCAGTGCCCAAAGTGTGAGAATCTTCTTCCTCAGCTAGCTGATTACTCTATGTATCAGTGTGGTGCTGTTCTTAGAGTACAAGGATTACAGATTGTGACTGGGATTACAGATGGATGGAGTACCCAGCTCATGCAAGAACTTCGAGACATCCCTTGGATCAGTCGTTGGCTACATATGGAGACCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

166

Amino Acids

18.6

Weight (kDa)

6.89

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR4-like_1st PF22910 100 - 126 3e-13 Enhanced disease resistance 4-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 476
AcsI RAATTY 2 cut(s) 92, 189
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 3 cut(s) 237, 390, 433
AfiI CCNNNNNNNGG 1 cut(s) 247
AflII CTTAAG 1 cut(s) 59
AgsI TTSAA 2 cut(s) 91, 275
AjnI CCWGG 2 cut(s) 9, 96
AluBI AGCT 5 cut(s) 50, 232, 346, 350, 439
AluI AGCT 5 cut(s) 50, 232, 346, 350, 439
Alw26I GTCTC 2 cut(s) 450, 485
AlwI GGATC 1 cut(s) 476
AlwNI CAGNNNCTG 2 cut(s) 16, 71
Ama87I CYCGRG 1 cut(s) 21
AoxI GGCC 2 cut(s) 132, 224
ApeKI GCWGC 2 cut(s) 16, 146
ApoI RAATTY 2 cut(s) 92, 189
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspS9I GGNCC 1 cut(s) 133
AsuNHI GCTAGC 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 3, 133
BccI CCATC 2 cut(s) 416, 420
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 2 cut(s) 11, 98
BcoDI GTCTC 2 cut(s) 450, 485
BfaI CTAG 1 cut(s) 347
BfrI CTTAAG 1 cut(s) 59
BisI GCNGC 2 cut(s) 17, 147
BlsI GCNGC 2 cut(s) 18, 148
Bme1390I CCNGG 2 cut(s) 11, 98
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 2 cut(s) 11, 98
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 1 cut(s) 274
BmtI GCTAGC 1 cut(s) 350
BmuI ACTGGG 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 1 cut(s) 464
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 415
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 2 cut(s) 11, 98
BseDI CCNNGG 1 cut(s) 464
BseGI GGATG 4 cut(s) 127, 265, 431, 459
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 3 cut(s) 213, 303, 356
BseNI ACTGG 1 cut(s) 415
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 2 cut(s) 3, 133
BseYI CCCAGC 1 cut(s) 435
BshFI GGCC 2 cut(s) 134, 226
BsiHKCI CYCGRG 1 cut(s) 21
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 2 cut(s) 450, 485
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 2 cut(s) 134, 226
Bso31I GGTCTC 1 cut(s) 485
BsoBI CYCGRG 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 1 cut(s) 468
BspANI GGCC 2 cut(s) 134, 226
BspCNI CTCAG 3 cut(s) 212, 302, 355
BspLI GGNNCC 1 cut(s) 135
BspOI GCTAGC 1 cut(s) 350
BspPI GGATC 1 cut(s) 476
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 485
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 1 cut(s) 415
BssECI CCNNGG 1 cut(s) 464
BssMI GATC 1 cut(s) 468
BssT1I CCWWGG 1 cut(s) 464
Bst2UI CCWGG 2 cut(s) 11, 98
Bst4CI ACNGT 1 cut(s) 105
BstAFI CTTAAG 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 4 cut(s) 199, 289, 342, 383
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 4 cut(s) 127, 265, 431, 459
BstKTI GATC 1 cut(s) 471
BstMAI GTCTC 2 cut(s) 450, 485
BstMBI GATC 1 cut(s) 468
BstNI CCWGG 2 cut(s) 11, 98
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 2 cut(s) 9, 96
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 3, 133
BsuRI GGCC 2 cut(s) 134, 226
BtsCI GGATG 4 cut(s) 127, 265, 431, 459
BtsIMutI CAGTG 3 cut(s) 101, 320, 374
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 2 cut(s) 16, 71
Cfr13I GGNCC 1 cut(s) 133
CsiI ACCWGGT 1 cut(s) 9
Csp6I GTAC 3 cut(s) 236, 389, 432
CviAII CATG 1 cut(s) 442
CviQI GTAC 3 cut(s) 236, 389, 432
DdeI CTNAG 4 cut(s) 199, 289, 342, 383
DpnI GATC 1 cut(s) 470
DpnII GATC 1 cut(s) 468
DraIII CACNNNGTG 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 464
Eco31I GGTCTC 1 cut(s) 485
Eco32I GATATC 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 21
Eco91I GGTNACC 1 cut(s) 5
EcoO109I RGGNCCY 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 189
EcoRII CCWGG 2 cut(s) 9, 96
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 464
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 1 cut(s) 445
FaiI YATR 6 cut(s) 111, 183, 362, 443, 486, 488
FaqI GGGAC 1 cut(s) 299
FatI CATG 1 cut(s) 441
FauNDI CATATG 1 cut(s) 486
Fnu4HI GCNGC 2 cut(s) 17, 147
FokI GGATG 4 cut(s) 134, 252, 438, 446
Fsp4HI GCNGC 2 cut(s) 17, 147
FspBI CTAG 1 cut(s) 347
GluI GCNGC 2 cut(s) 17, 147
GsaI CCCAGC 1 cut(s) 439
HaeIII GGCC 2 cut(s) 134, 226
HapII CCGG 1 cut(s) 227
Hin1II CATG 1 cut(s) 445
HinfI GANTC 8 cut(s) 26, 68, 140, 164, 204, 251, 287, 331
HpaII CCGG 1 cut(s) 227
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 46, 209
Hpy188III TCNNGA 2 cut(s) 201, 455
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 124, 169
HpyCH4III ACNGT 1 cut(s) 105
HpyCH4V TGCA 1 cut(s) 445
HpyF3I CTNAG 4 cut(s) 199, 289, 342, 383
Hsp92II CATG 1 cut(s) 445
Kzo9I GATC 1 cut(s) 468
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 2 cut(s) 3, 133
LweI GCATC 1 cut(s) 274
MabI ACCWGGT 1 cut(s) 9
MaeI CTAG 1 cut(s) 347
MaeIII GTNAC 2 cut(s) 5, 406
MalI GATC 1 cut(s) 470
MboI GATC 1 cut(s) 468
MboII GAAGA 2 cut(s) 326, 329
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 2 cut(s) 92, 189
MlyI GAGTC 3 cut(s) 134, 245, 281
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 2 cut(s) 297, 351
MseI TTAA 2 cut(s) 60, 221
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 11, 98
MvaI CCWGG 2 cut(s) 11, 98
NdeI CATATG 1 cut(s) 486
NdeII GATC 1 cut(s) 468
NheI GCTAGC 1 cut(s) 346
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 1 cut(s) 135
NmuCI GTSAC 2 cut(s) 5, 406
PfeI GAWTC 5 cut(s) 26, 68, 164, 204, 331
PkrI GCNGC 2 cut(s) 18, 148
PleI GAGTC 3 cut(s) 134, 245, 281
PpsI GAGTC 3 cut(s) 134, 245, 281
Psp6I CCWGG 2 cut(s) 9, 96
PspEI GGTNACC 1 cut(s) 5
PspFI CCCAGC 1 cut(s) 435
PspGI CCWGG 2 cut(s) 9, 96
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 133
PstNI CAGNNNCTG 2 cut(s) 16, 71
RsaI GTAC 3 cut(s) 237, 390, 433
RsaNI GTAC 3 cut(s) 236, 389, 432
SaqAI TTAA 2 cut(s) 60, 221
SatI GCNGC 2 cut(s) 17, 147
Sau3AI GATC 1 cut(s) 468
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 3 cut(s) 134, 245, 281
ScrFI CCNGG 2 cut(s) 11, 98
SduI GDGCHC 1 cut(s) 320
SetI ASST 8 cut(s) 15, 52, 234, 295, 308, 348, 352, 441
SexAI ACCWGGT 1 cut(s) 9
SfaNI GCATC 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 2 cut(s) 92, 189
SspI AATATT 1 cut(s) 258
SspMI CTAG 1 cut(s) 347
StyD4I CCNGG 2 cut(s) 9, 96
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 454
TasI AATT 2 cut(s) 92, 189
TatI WGTACW 1 cut(s) 388
TfiI GAWTC 5 cut(s) 26, 68, 164, 204, 331
Tru1I TTAA 2 cut(s) 60, 221
Tru9I TTAA 2 cut(s) 60, 221
TscAI CASTG 3 cut(s) 108, 320, 374
TseFI GTSAC 2 cut(s) 5, 406
TseI GCWGC 2 cut(s) 16, 146
Tsp45I GTSAC 2 cut(s) 5, 406
TspRI CASTG 3 cut(s) 108, 320, 374
Vha464I CTTAAG 1 cut(s) 59
XapI RAATTY 2 cut(s) 92, 189
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.