MD01G1169900.v1.1

Probable zinc-ribbon domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002425
Physical Location & Seq
Reverse (-)
27350599 .. 27351713
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1169900.v1.1.491

Sequence Viewer

Length: 402 bp
ATGTCTCCTGAATTGACAACACATGTTCGACTGGTTCGATGTCCGGAATGCCGGCAGCTTCTTGCGGAGTTGCCCCAAACTCCGGTCTACAAGTGTGGCGGATGTGGCGTCACTCTCCAAGCGAAAAGTCGAATAAATGGACCGAGGAGCAAGAGCGCTAGCGTAAATGACACTGAGGCAGCTCAGAGGATTGGTTTCGATCACGCTTCTGAAGATGGAAAATCCAGAAGCTCAGGTCGTAATGCAGCTCTTCTCGAATCCGGAGAATGCTCTTCAGACCAAGACAATGAGTGGGATCAGATTATGTCTTCCGAAGGCAATGAGTCTGGAAATTGGTTTTGTGATGTGTTGTGTTTGTATGGTAGAAGTTTTTGTTTAGGGTCGATTCAGGTCGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zinc_ribbon_12

Protein Analysis

134

Amino Acids

14.39

Weight (kDa)

5.14

Isoelectric Point (pI)

62.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR4-like_1st PF22910 8 - 41 8.5e-18 Enhanced disease resistance 4-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 87
AccIII TCCGGA 2 cut(s) 43, 260
AciI CCGC 2 cut(s) 65, 99
AclWI GGATC 1 cut(s) 303
AcuI CTGAAG 2 cut(s) 231, 258
AcyI GRCGYC 1 cut(s) 108
AfeI AGCGCT 1 cut(s) 157
AfiI CCNNNNNNNGG 1 cut(s) 82
AflIII ACRYGT 1 cut(s) 22
AluBI AGCT 4 cut(s) 58, 182, 231, 248
AluI AGCT 4 cut(s) 58, 182, 231, 248
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 303
Aor13HI TCCGGA 2 cut(s) 43, 260
Aor51HI AGCGCT 1 cut(s) 157
ApeKI GCWGC 3 cut(s) 55, 179, 245
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 140
AsuNHI GCTAGC 1 cut(s) 158
AvaII GGWCC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 67, 191, 257
BccI CCATC 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 159
BisI GCNGC 3 cut(s) 56, 180, 246
BlsI GCNGC 3 cut(s) 57, 181, 247
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmtI GCTAGC 1 cut(s) 162
BpiI GAAGAC 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 232
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 143
BsaWI WCCGGW 3 cut(s) 43, 82, 260
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 36
Bse3DI GCAATG 1 cut(s) 325
BseAI TCCGGA 2 cut(s) 43, 260
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 2 cut(s) 107, 401
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 3 cut(s) 165, 197, 246
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 160
BseXI GCAGC 3 cut(s) 67, 191, 257
BsiSI CCGG 4 cut(s) 44, 52, 83, 261
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 9
BsmI GAATGC 2 cut(s) 53, 272
Bsp13I TCCGGA 2 cut(s) 43, 260
Bsp143I GATC 2 cut(s) 199, 295
BspACI CCGC 2 cut(s) 65, 99
BspCNI CTCAG 3 cut(s) 166, 196, 245
BspEI TCCGGA 2 cut(s) 43, 260
BspOI GCTAGC 1 cut(s) 162
BspPI GGATC 1 cut(s) 303
BspQI GCTCTTC 2 cut(s) 255, 277
BsrDI GCAATG 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 1 cut(s) 36
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 2 cut(s) 199, 295
BssNI GRCGYC 1 cut(s) 108
Bst6I CTCTTC 2 cut(s) 255, 277
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 53, 160
BstDEI CTNAG 3 cut(s) 174, 183, 232
BstF5I GGATG 2 cut(s) 107, 401
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 2 cut(s) 202, 298
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 199, 295
BstMWI GCNNNNNNNGC 1 cut(s) 105
BstNSI RCATGY 1 cut(s) 26
BstV1I GCAGC 3 cut(s) 67, 191, 257
BstV2I GAAGAC 1 cut(s) 300
BtsCI GGATG 2 cut(s) 107, 401
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 2 cut(s) 53, 160
CfoI GCGC 1 cut(s) 158
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 140
CseI GACGC 1 cut(s) 97
CviAII CATG 1 cut(s) 23
CviJI RGCY 4 cut(s) 58, 182, 231, 248
CviKI_1 RGCY 4 cut(s) 58, 182, 231, 248
DdeI CTNAG 3 cut(s) 174, 183, 232
DpnI GATC 2 cut(s) 201, 297
DpnII GATC 2 cut(s) 199, 295
Eam1104I CTCTTC 2 cut(s) 255, 277
EarI CTCTTC 2 cut(s) 255, 277
EciI GGCGGA 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 140
Eco47III AGCGCT 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 231, 258
FaeI CATG 1 cut(s) 26
FaiI YATR 3 cut(s) 24, 305, 360
FatI CATG 1 cut(s) 22
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 3 cut(s) 56, 180, 246
FokI GGATG 1 cut(s) 114
Fsp4HI GCNGC 3 cut(s) 56, 180, 246
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 157
GluI GCNGC 3 cut(s) 56, 180, 246
HaeII RGCGCY 1 cut(s) 159
HapII CCGG 4 cut(s) 44, 52, 83, 261
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 158
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 1 cut(s) 26
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HinfI GANTC 3 cut(s) 257, 323, 385
HpaII CCGG 4 cut(s) 44, 52, 83, 261
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 6 cut(s) 186, 211, 277, 300, 313, 395
Hpy188III TCNNGA 6 cut(s) 8, 44, 225, 254, 261, 327
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 1 cut(s) 308
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpyF3I CTNAG 3 cut(s) 174, 183, 232
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 1 cut(s) 26
HspAI GCGC 1 cut(s) 156
Kpn2I TCCGGA 2 cut(s) 43, 260
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
Kzo9I GATC 2 cut(s) 199, 295
LguI GCTCTTC 2 cut(s) 255, 277
LmnI GCTCC 1 cut(s) 147
Lsp1109I GCAGC 3 cut(s) 67, 191, 257
MaeI CTAG 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 201, 297
MboI GATC 2 cut(s) 199, 295
MboII GAAGA 4 cut(s) 224, 242, 264, 300
MluCI AATT 2 cut(s) 11, 331
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 373
MnlI CCTC 3 cut(s) 138, 169, 180
MroI TCCGGA 2 cut(s) 43, 260
MroNI GCCGGC 1 cut(s) 51
MseI TTAA 1 cut(s) 400
MspI CCGG 4 cut(s) 44, 52, 83, 261
Mva1269I GAATGC 2 cut(s) 53, 272
MwoI GCNNNNNNNGC 1 cut(s) 105
NaeI GCCGGC 1 cut(s) 53
NdeII GATC 2 cut(s) 199, 295
NgoMIV GCCGGC 1 cut(s) 51
NheI GCTAGC 1 cut(s) 158
NlaIII CATG 1 cut(s) 26
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 26
PciI ACATGT 1 cut(s) 22
PciSI GCTCTTC 2 cut(s) 255, 277
PcsI WCGNNNNNNNCGW 1 cut(s) 34
PctI GAATGC 2 cut(s) 53, 272
PdiI GCCGGC 1 cut(s) 53
PfeI GAWTC 2 cut(s) 257, 385
PkrI GCNGC 3 cut(s) 57, 181, 247
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PscI ACATGT 1 cut(s) 22
PspPI GGNCC 1 cut(s) 140
SapI GCTCTTC 2 cut(s) 255, 277
SaqAI TTAA 1 cut(s) 400
SatI GCNGC 3 cut(s) 56, 180, 246
Sau3AI GATC 2 cut(s) 199, 295
Sau96I GGNCC 1 cut(s) 140
SchI GAGTC 1 cut(s) 332
SetI ASST 6 cut(s) 60, 184, 233, 238, 250, 393
SinI GGWCC 1 cut(s) 140
Sse9I AATT 2 cut(s) 11, 331
SsiI CCGC 2 cut(s) 65, 99
SspMI CTAG 1 cut(s) 159
TaqI TCGA 6 cut(s) 28, 37, 130, 198, 255, 383
TaqII GACCGA 1 cut(s) 157
TasI AATT 2 cut(s) 11, 331
TfiI GAWTC 2 cut(s) 257, 385
Tru1I TTAA 1 cut(s) 400
Tru9I TTAA 1 cut(s) 400
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 3 cut(s) 55, 179, 245
Tsp45I GTSAC 1 cut(s) 109
TspRI CASTG 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 140
XceI RCATGY 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 87
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.