MD01G1228600.v1.1

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004548
Physical Location & Seq
Reverse (-)
31825319 .. 31825876
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1228600.v1.1.491

Sequence Viewer

Length: 558 bp
ATGTTACTCTCCCTTCTCAATCTCCTCAGAATCATCAAGACACACTACTACTCGTTCATGTTTATGTTACGTGATGATCAGTATCACAAGCTTCCGAGAATAAATTATTCTGACTGTGTTGCGCTACAAGATCACAATAATTTATTCCCGGAAGTAGCCCCTTCGCCTTGGTTGGTGCCGTTACCGGTCCACTGCATAGCTGAATCCGTGAAGAACCAACTCCCGGTGGTGAAATATAGAGGAGGCAACCAGCGGCATTCGGATCACGACTCGCACACCGATACGTGTATTGTGTGCATGGATAGAGTGGAAGGAGGGCAAAGGGTGAGAGAGCTTTCCAATTGCTCACATGTATTTCACAAAGAGTGCCTAGATGCTTGGATTGATGAGGGTCAGTTTACCTGTCCACTGTGTCGATCCAGTCTATTGCCAAAGAATTGGACTACTCCAGAATCAAAAGGTCAACGGGAAAATGAGAGAGAATTTGGATGTCTGGATCCATGGAGGGCAGAGAGGATGGTTTACTTGTTTGGTGATGATTACCAGTTCATGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

186

Amino Acids

21.65

Weight (kDa)

6.12

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 93 - 139 3.4e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 94 - 139 1.3e-12 Ring finger domain
zf-C3HC4_2 PF13923 96 - 138 6.5e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 96 - 124 1.8e-08 RING-like zinc finger
zf-C3HC4 PF00097 96 - 138 3.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 96 - 136 1.8e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 175
AccB7I CCANNNNNTGG 1 cut(s) 550
AciI CCGC 1 cut(s) 253
AclWI GGATC 4 cut(s) 270, 411, 491, 504
AcsI RAATTY 1 cut(s) 482
AfiI CCNNNNNNNGG 2 cut(s) 223, 550
AflIII ACRYGT 2 cut(s) 284, 349
AgeI ACCGGT 1 cut(s) 184
AluBI AGCT 3 cut(s) 91, 200, 334
AluI AGCT 3 cut(s) 91, 200, 334
AlwI GGATC 4 cut(s) 270, 411, 491, 504
ApoI RAATTY 1 cut(s) 482
AsiGI ACCGGT 1 cut(s) 184
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 187
AsuC2I CCSGG 2 cut(s) 149, 224
AsuHPI GGTGA 3 cut(s) 241, 337, 545
AvaII GGWCC 1 cut(s) 187
BamHI GGATCC 1 cut(s) 496
BanI GGYRCC 1 cut(s) 175
BccI CCATC 1 cut(s) 511
BceAI ACGGC 1 cut(s) 163
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 2 cut(s) 149, 224
BfaI CTAG 1 cut(s) 371
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
Bme1390I CCNGG 2 cut(s) 149, 224
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 2 cut(s) 177, 498
BmrFI CCNGG 2 cut(s) 149, 224
BmsI GCATC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 432
BpuMI CCSGG 2 cut(s) 149, 224
BsaAI YACGTR 2 cut(s) 71, 285
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 167, 500
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 550
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 420, 544
Bse8I GATNNNNATC 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 167, 500
BseGI GGATG 2 cut(s) 494, 522
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 223, 550
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 2 cut(s) 420, 544
BseRI GAGGAG 2 cut(s) 14, 255
BshNI GGYRCC 1 cut(s) 175
BshTI ACCGGT 1 cut(s) 184
BsiSI CCGG 3 cut(s) 149, 185, 224
BslI CCNNNNNNNGG 2 cut(s) 223, 550
BsmI GAATGC 1 cut(s) 256
Bsp143I GATC 5 cut(s) 76, 130, 262, 416, 496
Bsp19I CCATGG 1 cut(s) 500
BspACI CCGC 1 cut(s) 253
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 177, 498
BspPI GGATC 4 cut(s) 270, 411, 491, 504
BspT107I GGYRCC 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 2 cut(s) 420, 544
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 167, 500
BssMI GATC 5 cut(s) 76, 130, 262, 416, 496
BssT1I CCWWGG 2 cut(s) 167, 500
Bst4CI ACNGT 2 cut(s) 116, 411
BstBAI YACGTR 2 cut(s) 71, 285
BstDEI CTNAG 1 cut(s) 26
BstDSI CCRYGG 1 cut(s) 500
BstF5I GGATG 2 cut(s) 494, 522
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 5 cut(s) 79, 133, 265, 419, 499
BstMBI GATC 5 cut(s) 76, 130, 262, 416, 496
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 147, 222
BstX2I RGATCY 1 cut(s) 496
BstXI CCANNNNNNTGG 1 cut(s) 438
BstYI RGATCY 1 cut(s) 496
BtgI CCRYGG 1 cut(s) 500
BtsCI GGATG 2 cut(s) 494, 522
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 190, 407
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 1 cut(s) 187
CspAI ACCGGT 1 cut(s) 184
CviAII CATG 5 cut(s) 58, 298, 350, 501, 550
CviJI RGCY 4 cut(s) 91, 158, 200, 334
CviKI_1 RGCY 4 cut(s) 91, 158, 200, 334
DdeI CTNAG 1 cut(s) 26
DpnI GATC 5 cut(s) 78, 132, 264, 418, 498
DpnII GATC 5 cut(s) 76, 130, 262, 416, 496
Eco130I CCWWGG 2 cut(s) 167, 500
Eco47I GGWCC 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 167, 500
ErhI CCWWGG 2 cut(s) 167, 500
FaeI CATG 5 cut(s) 61, 301, 353, 504, 553
FaiI YATR 8 cut(s) 59, 65, 197, 237, 299, 351, 502, 551
FatI CATG 5 cut(s) 57, 297, 349, 500, 549
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 2 cut(s) 501, 529
Fsp4HI GCNGC 1 cut(s) 254
FspBI CTAG 1 cut(s) 371
GlaI GCGC 1 cut(s) 123
GluI GCNGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 432
HapII CCGG 3 cut(s) 149, 185, 224
HhaI GCGC 1 cut(s) 124
Hin1II CATG 5 cut(s) 61, 301, 353, 504, 553
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HincII GTYRAC 1 cut(s) 464
HindII GTYRAC 1 cut(s) 464
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 4 cut(s) 30, 203, 269, 452
HpaII CCGG 3 cut(s) 149, 185, 224
HphI GGTGA 3 cut(s) 241, 337, 545
Hpy166II GTNNAC 5 cut(s) 190, 399, 407, 464, 523
Hpy188I TCNGA 4 cut(s) 29, 96, 112, 262
Hpy188III TCNNGA 4 cut(s) 37, 266, 449, 494
Hpy8I GTNNAC 5 cut(s) 190, 399, 407, 464, 523
HpyAV CCTTC 3 cut(s) 23, 171, 305
HpyCH4III ACNGT 2 cut(s) 116, 411
HpyCH4IV ACGT 2 cut(s) 70, 284
HpyCH4V TGCA 2 cut(s) 195, 297
HpyF3I CTNAG 1 cut(s) 26
HpySE526I ACGT 2 cut(s) 70, 284
Hsp92II CATG 5 cut(s) 61, 301, 353, 504, 553
HspAI GCGC 1 cut(s) 122
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 5 cut(s) 76, 130, 262, 416, 496
LpnPI CCDG 8 cut(s) 162, 198, 237, 263, 415, 433, 462, 479
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 371
MaeII ACGT 2 cut(s) 70, 284
MaeIII GTNAC 3 cut(s) 3, 66, 180
MalI GATC 5 cut(s) 78, 132, 264, 418, 498
MboI GATC 5 cut(s) 76, 130, 262, 416, 496
MboII GAAGA 1 cut(s) 223
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 496
MluCI AATT 5 cut(s) 103, 139, 340, 436, 482
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 7 cut(s) 35, 233, 236, 308, 382, 498, 507
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 3 cut(s) 149, 185, 224
MspR9I CCNGG 2 cut(s) 149, 224
MunI CAATTG 1 cut(s) 340
Mva1269I GAATGC 1 cut(s) 256
NciI CCSGG 2 cut(s) 149, 224
NcoI CCATGG 1 cut(s) 500
NdeII GATC 5 cut(s) 76, 130, 262, 416, 496
NlaIII CATG 5 cut(s) 61, 301, 353, 504, 553
NlaIV GGNNCC 2 cut(s) 177, 498
NspI RCATGY 1 cut(s) 353
PciI ACATGT 1 cut(s) 349
PctI GAATGC 1 cut(s) 256
PfeI GAWTC 3 cut(s) 30, 203, 452
PflMI CCANNNNNTGG 1 cut(s) 550
PfoI TCCNGGA 1 cut(s) 147
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 1 cut(s) 255
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Ppu21I YACGTR 2 cut(s) 71, 285
PscI ACATGT 1 cut(s) 349
PspN4I GGNNCC 2 cut(s) 177, 498
PspPI GGNCC 1 cut(s) 187
PsuI RGATCY 1 cut(s) 496
RseI CAYNNNNRTG 1 cut(s) 62
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 5 cut(s) 76, 130, 262, 416, 496
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 2 cut(s) 149, 224
SetI ASST 7 cut(s) 73, 93, 202, 287, 336, 404, 463
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 62
Sse9I AATT 5 cut(s) 103, 139, 340, 436, 482
SsiI CCGC 1 cut(s) 253
SspMI CTAG 1 cut(s) 371
StyD4I CCNGG 2 cut(s) 147, 222
StyI CCWWGG 2 cut(s) 167, 500
TaaI ACNGT 2 cut(s) 116, 411
TaiI ACGT 2 cut(s) 73, 287
TaqI TCGA 1 cut(s) 415
TasI AATT 5 cut(s) 103, 139, 340, 436, 482
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 3 cut(s) 30, 203, 452
TscAI CASTG 2 cut(s) 197, 414
TspDTI ATGAA 2 cut(s) 46, 538
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 2 cut(s) 197, 414
Van91I CCANNNNNTGG 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 1 cut(s) 482
XceI RCATGY 1 cut(s) 353
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.