Rroxscaffold_4G00277780

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004548
Physical Location & Seq
Forward (+)
1064947 .. 1065462
516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277780.1

Sequence Viewer

Length: 516 bp
ATGAAGAAGATGATAGTATACTCATTGTTCAGACCTGAAGTTAAAGACAAGGTCGACACTAATGATCATCATCACCAGGTCGAGATGAATTTCCCAGCTGCAGCCGCTCCGAATTGGTTGCATTTGCAGGTACCAGTAGTTCCGGTGGTAGTCGACACTGAACCGGTTAAGAAGCAACTTCCGGTTCTGCAATACCGGAACCTAATGATAGAGAGAAGAGATCGATCGCGACTTAGAGATCATGAGGACGACGGGTACTCTATGTGTATAGTGTGCATGAATAGCATAGAGGCTATGGATGGAGTAAGAGAGCTGAAGAACTGTTCCCACATCTTTCATAGAGAGTGCTTGGATCTTTGGGTTGCTGAGGGTCAGGTAACCTGTCCTCTGTGCAGGTCTAAGCTATTGCCAACTGCTACGGCTACTCCAAATGAACATTGGAAACCTGAAGGAGGGGATCCATGGAGATCGGAGAGGATGATTTACTTGTTCGGTGATGATTACGTCTTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

171

Amino Acids

19.96

Weight (kDa)

6.05

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 88 - 132 2.8e-11 Ring finger domain
zf-C3HC4_2 PF13923 88 - 131 1.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 89 - 131 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 89 - 117 2.2e-07 RING-like zinc finger
zf-rbx1 PF12678 89 - 132 7.8e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 118, 384
Acc65I GGTACC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 130
AccBSI CCGCTC 1 cut(s) 107
AccI GTMKAC 3 cut(s) 18, 54, 153
AccII CGCG 1 cut(s) 229
AciI CCGC 1 cut(s) 105
AclWI GGATC 3 cut(s) 360, 452, 465
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 3 cut(s) 57, 335, 468
AfaI GTAC 2 cut(s) 132, 257
AfiI CCNNNNNNNGG 1 cut(s) 452
AgeI ACCGGT 1 cut(s) 163
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 3 cut(s) 98, 313, 403
AluI AGCT 3 cut(s) 98, 313, 403
AlwI GGATC 3 cut(s) 360, 452, 465
ApeKI GCWGC 2 cut(s) 98, 101
ApoI RAATTY 1 cut(s) 88
AsiGI ACCGGT 1 cut(s) 163
Asp718I GGTACC 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 65, 506
BamHI GGATCC 1 cut(s) 457
BanI GGYRCC 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 499
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 85, 113
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 435
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 1 cut(s) 77
BclI TGATCA 1 cut(s) 64
BfmI CTRYAG 1 cut(s) 99
BfuAI ACCTGC 2 cut(s) 118, 384
BisI GCNGC 3 cut(s) 99, 102, 105
BlsI GCNGC 3 cut(s) 100, 103, 106
Bme1390I CCNGG 1 cut(s) 77
BmiI GGNNCC 3 cut(s) 132, 200, 459
BmrFI CCNGG 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 499
Bpu10I CCTNAGC 1 cut(s) 366
Bsa29I ATCGAT 1 cut(s) 223
BsaBI GATNNNNATC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 461
BsaWI WCCGGW 4 cut(s) 142, 163, 181, 195
BsaXI ACNNNNNCTCC 2 cut(s) 409, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 2 cut(s) 134, 511
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 77
BseCI ATCGAT 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 2 cut(s) 304, 483
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 134, 511
BseXI GCAGC 2 cut(s) 85, 113
BseYI CCCAGC 1 cut(s) 94
BsgI GTGCAG 1 cut(s) 412
Bsh1236I CGCG 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 130
BshTI ACCGGT 1 cut(s) 163
BshVI ATCGAT 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 227
BsiSI CCGG 4 cut(s) 143, 164, 182, 196
BslI CCNNNNNNNGG 1 cut(s) 452
Bsp143I GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
Bsp19I CCATGG 1 cut(s) 461
Bsp68I TCGCGA 1 cut(s) 229
BspACI CCGC 1 cut(s) 105
BspCNI CTCAG 1 cut(s) 358
BspDI ATCGAT 1 cut(s) 223
BspFNI CGCG 1 cut(s) 229
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 132, 200, 459
BspMAI CTGCAG 1 cut(s) 103
BspMI ACCTGC 2 cut(s) 118, 384
BspPI GGATC 3 cut(s) 360, 452, 465
BspT107I GGYRCC 1 cut(s) 130
BsrBI CCGCTC 1 cut(s) 107
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 2 cut(s) 134, 511
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
BssNAI GTATAC 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 461
Bst1107I GTATAC 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 1 cut(s) 211
BstDEI CTNAG 3 cut(s) 233, 366, 399
BstDSI CCRYGG 1 cut(s) 461
BstEII GGTNACC 1 cut(s) 376
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 2 cut(s) 304, 483
BstFNI CGCG 1 cut(s) 229
BstKTI GATC 7 cut(s) 67, 223, 227, 241, 355, 460, 470
BstMBI GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 2 cut(s) 104, 282
BstNI CCWGG 1 cut(s) 77
BstPI GGTNACC 1 cut(s) 376
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 2 cut(s) 85, 113
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 2 cut(s) 352, 457
BstYI RGATCY 2 cut(s) 352, 457
BstZ17I GTATAC 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 223
BsuTUI ATCGAT 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 461
BtsCI GGATG 2 cut(s) 304, 483
BtsIMutI CAGTG 1 cut(s) 156
BtuMI TCGCGA 1 cut(s) 229
BveI ACCTGC 2 cut(s) 118, 384
CciI TCATGA 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 223
CsiI ACCWGGT 1 cut(s) 75
Csp6I GTAC 2 cut(s) 131, 256
CspAI ACCGGT 1 cut(s) 163
CviAII CATG 3 cut(s) 242, 277, 462
CviJI RGCY 6 cut(s) 98, 104, 293, 313, 403, 422
CviKI_1 RGCY 6 cut(s) 98, 104, 293, 313, 403, 422
CviQI GTAC 2 cut(s) 131, 256
DdeI CTNAG 3 cut(s) 233, 366, 399
DpnI GATC 7 cut(s) 66, 222, 226, 240, 354, 459, 469
DpnII GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 461
Eco57I CTGAAG 3 cut(s) 57, 335, 468
Eco91I GGTNACC 1 cut(s) 376
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 461
FaeI CATG 3 cut(s) 245, 280, 465
FaiI YATR 9 cut(s) 19, 243, 263, 269, 278, 287, 296, 339, 463
FatI CATG 3 cut(s) 241, 276, 461
FbaI TGATCA 1 cut(s) 64
FblI GTMKAC 3 cut(s) 18, 54, 153
Fnu4HI GCNGC 3 cut(s) 99, 102, 105
FokI GGATG 2 cut(s) 311, 490
Fsp4HI GCNGC 3 cut(s) 99, 102, 105
GluI GCNGC 3 cut(s) 99, 102, 105
GsaI CCCAGC 1 cut(s) 98
HapII CCGG 4 cut(s) 143, 164, 182, 196
Hin1II CATG 3 cut(s) 245, 280, 465
HincII GTYRAC 2 cut(s) 55, 154
HindII GTYRAC 2 cut(s) 55, 154
HpaII CCGG 4 cut(s) 143, 164, 182, 196
HphI GGTGA 2 cut(s) 65, 506
Hpy166II GTNNAC 3 cut(s) 19, 55, 154
Hpy188I TCNGA 3 cut(s) 32, 111, 472
Hpy188III TCNNGA 3 cut(s) 82, 228, 242
Hpy8I GTNNAC 3 cut(s) 19, 55, 154
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 6 cut(s) 101, 121, 127, 190, 276, 393
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 282
HpyF3I CTNAG 3 cut(s) 233, 366, 399
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 3 cut(s) 245, 280, 465
KpnI GGTACC 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 2 cut(s) 85, 113
MabI ACCWGGT 1 cut(s) 75
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 1 cut(s) 376
MalI GATC 7 cut(s) 66, 222, 226, 240, 354, 459, 469
MbiI CCGCTC 1 cut(s) 107
MboI GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
MboII GAAGA 5 cut(s) 16, 19, 228, 328, 499
MflI RGATCY 2 cut(s) 352, 457
MluCI AATT 2 cut(s) 88, 112
MnlI CCTC 6 cut(s) 238, 283, 361, 396, 446, 468
MseI TTAA 2 cut(s) 42, 168
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 4 cut(s) 143, 164, 182, 196
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 104, 282
NcoI CCATGG 1 cut(s) 461
NdeII GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
NlaIII CATG 3 cut(s) 245, 280, 465
NlaIV GGNNCC 3 cut(s) 132, 200, 459
NruI TCGCGA 1 cut(s) 229
PagI TCATGA 1 cut(s) 241
PflFI GACNNNGTC 1 cut(s) 50
PinAI ACCGGT 1 cut(s) 163
PkrI GCNGC 3 cut(s) 100, 103, 106
Ple19I CGATCG 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 75
PspEI GGTNACC 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 132, 200, 459
PsrI GAACNNNNNNTAC 2 cut(s) 123, 155
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 2 cut(s) 352, 457
PsyI GACNNNGTC 1 cut(s) 50
PvuI CGATCG 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 98
RruI TCGCGA 1 cut(s) 229
RsaI GTAC 2 cut(s) 132, 257
RsaNI GTAC 2 cut(s) 131, 256
SalI GTCGAC 2 cut(s) 53, 152
SaqAI TTAA 2 cut(s) 42, 168
SatI GCNGC 3 cut(s) 99, 102, 105
Sau3AI GATC 7 cut(s) 64, 220, 224, 238, 352, 457, 467
ScrFI CCNGG 1 cut(s) 77
SexAI ACCWGGT 1 cut(s) 75
SfcI CTRYAG 1 cut(s) 99
Sse9I AATT 2 cut(s) 88, 112
SsiI CCGC 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 1 cut(s) 507
TaqI TCGA 4 cut(s) 54, 81, 153, 223
TasI AATT 2 cut(s) 88, 112
TauI GCSGC 1 cut(s) 107
Tru1I TTAA 2 cut(s) 42, 168
Tru9I TTAA 2 cut(s) 42, 168
TscAI CASTG 1 cut(s) 163
TseI GCWGC 2 cut(s) 98, 101
TspDTI ATGAA 5 cut(s) 17, 101, 293, 326, 447
TspRI CASTG 1 cut(s) 163
Tth111I GACNNNGTC 1 cut(s) 50
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 435
XmiI GTMKAC 3 cut(s) 18, 54, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.