Rh1CG433100

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004548
Physical Location & Seq
Reverse (-)
68913643 .. 68914245
603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG433100.1

Sequence Viewer

Length: 603 bp
ATGAAGTTGATGTTGTTGCTGTGTGATCTTCTCAAACTTCTGCTCGAGAAGATCATCTACTATCCAGAATCATTCCTTAGACACAGTGAAGATGATCAACAAAGTAAGGACGATAGTGATCATCAGCTGGAGGTCATGAACTTTGAAGCTGCAGCAACTTCAGATCCTACTTGGTTGCACATGCAAGTACCAGTGGTTCCGGTGGTAAATATTGACACAGAATTCATTAAGAAGCAACTTCCGGTTGTGCTATACCGGGACCTAATTCTGAAGAAAACAAGCCGCCAACGCCAATTTCAAGCTACATCATCATCTTCGGAGCAGGAAATGCACATGTCATCATGTATTGTGTGCATGAATAGCATGGAGGGCAGCGACGAGGTGAGAGAGCTGTGCAATTGTGAGCATGTTTTCCACAGAGACTGCCTGGATGCTTGGATCGGTCAGCCTCAGCTAACCTGTCCTCTTTGTAGATCTGCGCTACTAATACCCGCTCCTACTTCTGCTACTACTTCAAAGCATGATAAAGATGGAGGAAGAGATCCAAGGAGGGCCGAGAGGATGATTTACTTGTTTGGTGAAGATATTTTCTTCACGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

200

Amino Acids

23.03

Weight (kDa)

5.22

Isoelectric Point (pI)

54.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 111 - 158 9.3e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 114 - 158 1.6e-10 Ring finger domain
zf-RING_11 PF17123 115 - 143 6e-06 RING-like zinc finger
zf-C3HC4_2 PF13923 115 - 157 8.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 115 - 157 9.8e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 494
AciI CCGC 2 cut(s) 283, 492
AclWI GGATC 3 cut(s) 158, 446, 536
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 2 cut(s) 144, 290
AfaI GTAC 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 3 cut(s) 146, 299, 516
AjnI CCWGG 1 cut(s) 426
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 5 cut(s) 127, 149, 302, 391, 454
AluI AGCT 5 cut(s) 127, 149, 302, 391, 454
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 3 cut(s) 158, 446, 536
AlwNI CAGNNNCTG 1 cut(s) 423
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 552
ApeKI GCWGC 3 cut(s) 149, 152, 372
ApoI RAATTY 1 cut(s) 221
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 2 cut(s) 259, 552
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 394, 590
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 259
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 136, 164, 384
BccI CCATC 1 cut(s) 524
BciT130I CCWGG 1 cut(s) 428
BclI TGATCA 2 cut(s) 94, 118
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 1 cut(s) 601
BfmI CTRYAG 1 cut(s) 150
BglII AGATCT 1 cut(s) 473
BisI GCNGC 4 cut(s) 150, 153, 283, 373
BlsI GCNGC 4 cut(s) 151, 154, 284, 374
Bme1390I CCNGG 2 cut(s) 257, 428
Bme18I GGWCC 1 cut(s) 259
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 259, 552
BmiI GGNNCC 2 cut(s) 198, 260
BmrFI CCNGG 2 cut(s) 257, 428
BmsI GCATC 1 cut(s) 421
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 450
BpuMI CCSGG 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 545
BsaWI WCCGGW 2 cut(s) 199, 241
Bse1I ACTGG 1 cut(s) 191
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 1 cut(s) 545
BseGI GGATG 2 cut(s) 436, 567
BseJI GATNNNNATC 1 cut(s) 117
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 1 cut(s) 191
BseXI GCAGC 3 cut(s) 136, 164, 384
BshFI GGCC 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 3 cut(s) 200, 242, 256
BslFI GGGAC 1 cut(s) 272
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 1 cut(s) 554
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
BspACI CCGC 2 cut(s) 283, 492
BspANI GGCC 1 cut(s) 554
BspCNI CTCAG 1 cut(s) 463
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 198, 260
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 3 cut(s) 158, 446, 536
BsrBI CCGCTC 1 cut(s) 494
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 545
BssMI GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
BssT1I CCWWGG 1 cut(s) 545
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 532
BstAPI GCANNNNNTGC 1 cut(s) 328
BstDEI CTNAG 2 cut(s) 77, 450
BstF5I GGATG 2 cut(s) 436, 567
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 9 cut(s) 28, 54, 97, 121, 166, 441, 476, 544, 600
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
BstMWI GCNNNNNNNGC 4 cut(s) 288, 328, 360, 369
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 3 cut(s) 184, 337, 410
BstSCI CCNGG 2 cut(s) 255, 426
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 136, 164, 384
BstX2I RGATCY 3 cut(s) 163, 473, 541
BstYI RGATCY 3 cut(s) 163, 473, 541
BsuRI GGCC 1 cut(s) 554
BtsCI GGATG 2 cut(s) 436, 567
BtsIMutI CAGTG 2 cut(s) 91, 198
CaiI CAGNNNCTG 1 cut(s) 423
CciI TCATGA 1 cut(s) 135
CfoI GCGC 1 cut(s) 481
Cfr13I GGNCC 2 cut(s) 259, 552
Csp6I GTAC 1 cut(s) 188
CviAII CATG 8 cut(s) 136, 181, 334, 342, 355, 364, 407, 521
CviJI RGCY 8 cut(s) 127, 149, 282, 302, 391, 448, 454, 554
CviKI_1 RGCY 8 cut(s) 127, 149, 282, 302, 391, 448, 454, 554
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 2 cut(s) 77, 450
DpnI GATC 9 cut(s) 27, 53, 96, 120, 165, 440, 475, 543, 599
DpnII GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
Eam1104I CTCTTC 1 cut(s) 532
EarI CTCTTC 1 cut(s) 532
Eco130I CCWWGG 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 144, 290
Eco88I CYCGRG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 426
EcoT14I CCWWGG 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 545
FaeI CATG 8 cut(s) 139, 184, 337, 345, 358, 367, 410, 524
FaiI YATR 9 cut(s) 137, 182, 253, 335, 343, 356, 365, 408, 522
FaqI GGGAC 1 cut(s) 272
FatI CATG 8 cut(s) 135, 180, 333, 341, 354, 363, 406, 520
FauI CCCGC 1 cut(s) 499
FbaI TGATCA 2 cut(s) 94, 118
Fnu4HI GCNGC 4 cut(s) 150, 153, 283, 373
FokI GGATG 2 cut(s) 443, 574
Fsp4HI GCNGC 4 cut(s) 150, 153, 283, 373
FspBI CTAG 1 cut(s) 601
GlaI GCGC 1 cut(s) 480
GluI GCNGC 4 cut(s) 150, 153, 283, 373
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 1 cut(s) 554
HapII CCGG 3 cut(s) 200, 242, 256
HhaI GCGC 1 cut(s) 481
Hin1II CATG 8 cut(s) 139, 184, 337, 345, 358, 367, 410, 524
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HinfI GANTC 1 cut(s) 68
HpaII CCGG 3 cut(s) 200, 242, 256
HphI GGTGA 2 cut(s) 394, 590
Hpy188I TCNGA 3 cut(s) 163, 270, 319
Hpy188III TCNNGA 4 cut(s) 46, 65, 136, 595
Hpy99I CGWCG 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 6 cut(s) 152, 178, 184, 331, 354, 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 288, 328, 360, 369
HpyF3I CTNAG 2 cut(s) 77, 450
Hsp92II CATG 8 cut(s) 139, 184, 337, 345, 358, 367, 410, 524
HspAI GCGC 1 cut(s) 479
Ksp22I TGATCA 2 cut(s) 94, 118
Kzo9I GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
LmnI GCTCC 2 cut(s) 319, 499
Lsp1109I GCAGC 3 cut(s) 136, 164, 384
LweI GCATC 1 cut(s) 421
MaeI CTAG 1 cut(s) 601
MalI GATC 9 cut(s) 27, 53, 96, 120, 165, 440, 475, 543, 599
MbiI CCGCTC 1 cut(s) 494
MboI GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
MboII GAAGA 8 cut(s) 20, 61, 101, 283, 306, 549, 583, 593
MfeI CAATTG 1 cut(s) 397
MflI RGATCY 3 cut(s) 163, 473, 541
MluCI AATT 4 cut(s) 221, 264, 293, 397
MnlI CCTC 8 cut(s) 124, 361, 373, 459, 474, 527, 543, 552
MseI TTAA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 3 cut(s) 200, 242, 256
MspR9I CCNGG 2 cut(s) 257, 428
MunI CAATTG 1 cut(s) 397
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 4 cut(s) 288, 328, 360, 369
NciI CCSGG 1 cut(s) 257
NdeII GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
NlaIII CATG 8 cut(s) 139, 184, 337, 345, 358, 367, 410, 524
NlaIV GGNNCC 2 cut(s) 198, 260
NmeAIII GCCGAG 1 cut(s) 580
NspI RCATGY 3 cut(s) 184, 337, 410
PaeR7I CTCGAG 1 cut(s) 44
PagI TCATGA 1 cut(s) 135
PciI ACATGT 1 cut(s) 333
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 4 cut(s) 151, 154, 284, 374
PpuMI RGGWCCY 1 cut(s) 259
PscI ACATGT 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 2 cut(s) 198, 260
PspPI GGNCC 2 cut(s) 259, 552
PspPPI RGGWCCY 1 cut(s) 259
PsrI GAACNNNNNNTAC 2 cut(s) 180, 212
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 423
PsuI RGATCY 3 cut(s) 163, 473, 541
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 150, 153, 283, 373
Sau3AI GATC 9 cut(s) 25, 51, 94, 118, 163, 438, 473, 541, 597
Sau96I GGNCC 2 cut(s) 259, 552
ScrFI CCNGG 2 cut(s) 257, 428
SetI ASST 9 cut(s) 129, 135, 151, 264, 304, 384, 393, 456, 461
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 1 cut(s) 150
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 1 cut(s) 259
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 4 cut(s) 221, 264, 293, 397
SsiI CCGC 2 cut(s) 283, 492
SspI AATATT 1 cut(s) 211
SspMI CTAG 1 cut(s) 601
StyD4I CCNGG 2 cut(s) 255, 426
StyI CCWWGG 1 cut(s) 545
TaaI ACNGT 1 cut(s) 86
TaqI TCGA 1 cut(s) 45
TaqII GACCGA 1 cut(s) 431
TasI AATT 4 cut(s) 221, 264, 293, 397
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 91, 198
TseI GCWGC 3 cut(s) 149, 152, 372
TspDTI ATGAA 4 cut(s) 17, 152, 214, 371
TspRI CASTG 2 cut(s) 91, 198
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 221
XceI RCATGY 3 cut(s) 184, 337, 410
XhoI CTCGAG 1 cut(s) 44
XspI CTAG 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.