Rh1CG433200

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004548
Physical Location & Seq
Reverse (-)
68915293 .. 68916289
997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG433200.1

Sequence Viewer

Length: 570 bp
ATGGATCAACTGAGAAACCTAGCTTTACTAATTTGTCATCATCTCATTCTACTCATGAAGAAGATGATCGAATACTCATTGTTCAGACCTGAAGTTAAAGACAAGGTCGACACTAATGATCATCATCATCAGGTCGAGATGAATTTCCCAGCTGCAGCCGCTCCGAATTGGTTGTACTTGCAGGTACCAGTAGTTCCGGTGGTAGTCGACACTGAATCGGTTAAGAAGCAACTTCCGGTTCTGCAATACCGGAACCTAATGATAGAGAGAAGAGATCGATCGCGATTTAGAGATCATGAGGACGACGGGTACTCTATGTGTATAGTGTGCATAAATAGCATAGAGGCTGTGGATGGAGTAAGAGAGCTGAAGAACTGTTCCCACGTCTTTCATAGAGAGTGCTTGGATCTTTGGGTTGCTGAGGGTCAGGTAACCTGTCCTCTGTGCAGGTCTAAGCTATTGCCAACTGCTACGGCTACTCCAAATGAACATTGGAAACCTGAAGGAGGGGATCCATGGAGATCGGAGAGGATGATTTACTTGTTCGGTGATGATTACGTCTTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

189

Amino Acids

22.09

Weight (kDa)

5.97

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 106 - 150 1.5e-10 Ring finger domain
zf-C3HC4_2 PF13923 106 - 149 9.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 107 - 149 1.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 107 - 150 5.1e-07 RING-H2 zinc finger domain
zf-RING_11 PF17123 107 - 135 2.6e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 172, 438
Acc65I GGTACC 1 cut(s) 184
AccB1I GGYRCC 1 cut(s) 184
AccBSI CCGCTC 1 cut(s) 161
AccI GTMKAC 2 cut(s) 108, 207
AccII CGCG 1 cut(s) 283
AciI CCGC 1 cut(s) 159
AclWI GGATC 4 cut(s) 12, 414, 506, 519
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 3 cut(s) 111, 389, 522
AfaI GTAC 3 cut(s) 176, 186, 311
AfiI CCNNNNNNNGG 1 cut(s) 506
AjiI CACGTC 1 cut(s) 385
AluBI AGCT 4 cut(s) 23, 152, 367, 457
AluI AGCT 4 cut(s) 23, 152, 367, 457
AlwI GGATC 4 cut(s) 12, 414, 506, 519
ApeKI GCWGC 2 cut(s) 152, 155
ApoI RAATTY 1 cut(s) 142
Asp718I GGTACC 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 560
BamHI GGATCC 1 cut(s) 511
BanI GGYRCC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 553
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 2 cut(s) 139, 167
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 489
BclI TGATCA 1 cut(s) 118
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 2 cut(s) 172, 438
BisI GCNGC 3 cut(s) 153, 156, 159
BlsI GCNGC 3 cut(s) 154, 157, 160
BmgBI CACGTC 1 cut(s) 385
BmiI GGNNCC 3 cut(s) 186, 254, 513
BpiI GAAGAC 1 cut(s) 553
Bpu10I CCTNAGC 1 cut(s) 420
Bsa29I ATCGAT 1 cut(s) 277
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 515
BsaWI WCCGGW 3 cut(s) 196, 235, 249
BsaXI ACNNNNNCTCC 2 cut(s) 463, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 188, 565
Bse8I GATNNNNATC 1 cut(s) 123
BseCI ATCGAT 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 515
BseGI GGATG 2 cut(s) 358, 537
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 2 cut(s) 188, 565
BseXI GCAGC 2 cut(s) 139, 167
BseYI CCCAGC 1 cut(s) 148
BsgI GTGCAG 1 cut(s) 466
Bsh1236I CGCG 1 cut(s) 283
Bsh1285I CGRYCG 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 277
BsiEI CGRYCG 1 cut(s) 281
BsiSI CCGG 3 cut(s) 197, 236, 250
BslI CCNNNNNNNGG 1 cut(s) 506
Bsp143I GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
Bsp19I CCATGG 1 cut(s) 515
Bsp68I TCGCGA 1 cut(s) 283
BspACI CCGC 1 cut(s) 159
BspCNI CTCAG 1 cut(s) 412
BspDI ATCGAT 1 cut(s) 277
BspFNI CGCG 1 cut(s) 283
BspHI TCATGA 2 cut(s) 54, 295
BspLI GGNNCC 3 cut(s) 186, 254, 513
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 2 cut(s) 172, 438
BspPI GGATC 4 cut(s) 12, 414, 506, 519
BspT107I GGYRCC 1 cut(s) 184
BsrBI CCGCTC 1 cut(s) 161
BsrI ACTGG 2 cut(s) 188, 565
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
BssT1I CCWWGG 1 cut(s) 515
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 1 cut(s) 265
BstDEI CTNAG 3 cut(s) 11, 420, 453
BstDSI CCRYGG 1 cut(s) 515
BstEII GGTNACC 1 cut(s) 430
BstENI CCTNNNNNAGG 1 cut(s) 504
BstF5I GGATG 2 cut(s) 358, 537
BstFNI CGCG 1 cut(s) 283
BstKTI GATC 9 cut(s) 7, 69, 121, 277, 281, 295, 409, 514, 524
BstMBI GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
BstMCI CGRYCG 1 cut(s) 281
BstMWI GCNNNNNNNGC 2 cut(s) 158, 336
BstPI GGTNACC 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 153
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 2 cut(s) 139, 167
BstV2I GAAGAC 1 cut(s) 553
BstX2I RGATCY 2 cut(s) 406, 511
BstYI RGATCY 2 cut(s) 406, 511
Bsu15I ATCGAT 1 cut(s) 277
BsuTUI ATCGAT 1 cut(s) 277
BtgI CCRYGG 1 cut(s) 515
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 2 cut(s) 358, 537
BtsIMutI CAGTG 1 cut(s) 210
BtuMI TCGCGA 1 cut(s) 283
BveI ACCTGC 2 cut(s) 172, 438
CciI TCATGA 2 cut(s) 54, 295
ClaI ATCGAT 1 cut(s) 277
Csp6I GTAC 3 cut(s) 175, 185, 310
CviAII CATG 3 cut(s) 55, 296, 516
CviJI RGCY 7 cut(s) 23, 152, 158, 347, 367, 457, 476
CviKI_1 RGCY 7 cut(s) 23, 152, 158, 347, 367, 457, 476
CviQI GTAC 3 cut(s) 175, 185, 310
DdeI CTNAG 3 cut(s) 11, 420, 453
DpnI GATC 9 cut(s) 6, 68, 120, 276, 280, 294, 408, 513, 523
DpnII GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 515
Eco57I CTGAAG 3 cut(s) 111, 389, 522
Eco91I GGTNACC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoO65I GGTNACC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 515
FaeI CATG 3 cut(s) 58, 299, 519
FaiI YATR 8 cut(s) 56, 297, 317, 323, 332, 341, 393, 517
FatI CATG 3 cut(s) 54, 295, 515
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 2 cut(s) 108, 207
Fnu4HI GCNGC 3 cut(s) 153, 156, 159
FokI GGATG 2 cut(s) 365, 544
Fsp4HI GCNGC 3 cut(s) 153, 156, 159
FspBI CTAG 1 cut(s) 20
GluI GCNGC 3 cut(s) 153, 156, 159
GsaI CCCAGC 1 cut(s) 152
HapII CCGG 3 cut(s) 197, 236, 250
Hin1II CATG 3 cut(s) 58, 299, 519
HincII GTYRAC 2 cut(s) 109, 208
HindII GTYRAC 2 cut(s) 109, 208
HinfI GANTC 1 cut(s) 215
HpaII CCGG 3 cut(s) 197, 236, 250
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 2 cut(s) 109, 208
Hpy188I TCNGA 3 cut(s) 86, 165, 526
Hpy188III TCNNGA 4 cut(s) 55, 136, 282, 296
Hpy8I GTNNAC 2 cut(s) 109, 208
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 2 cut(s) 384, 558
HpyCH4V TGCA 5 cut(s) 155, 181, 244, 330, 447
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 336
HpyF3I CTNAG 3 cut(s) 11, 420, 453
HpySE526I ACGT 2 cut(s) 384, 558
Hsp92II CATG 3 cut(s) 58, 299, 519
KpnI GGTACC 1 cut(s) 188
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 2 cut(s) 139, 167
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 384, 558
MaeIII GTNAC 1 cut(s) 430
MalI GATC 9 cut(s) 6, 68, 120, 276, 280, 294, 408, 513, 523
MbiI CCGCTC 1 cut(s) 161
MboI GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
MboII GAAGA 5 cut(s) 70, 73, 282, 382, 553
MflI RGATCY 2 cut(s) 406, 511
MluCI AATT 3 cut(s) 30, 142, 166
MnlI CCTC 6 cut(s) 292, 337, 415, 450, 500, 522
MseI TTAA 2 cut(s) 96, 222
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 3 cut(s) 197, 236, 250
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 2 cut(s) 158, 336
NcoI CCATGG 1 cut(s) 515
NdeII GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
NlaIII CATG 3 cut(s) 58, 299, 519
NlaIV GGNNCC 3 cut(s) 186, 254, 513
NruI TCGCGA 1 cut(s) 283
PagI TCATGA 2 cut(s) 54, 295
PfeI GAWTC 1 cut(s) 215
PflFI GACNNNGTC 1 cut(s) 104
PkrI GCNGC 3 cut(s) 154, 157, 160
Ple19I CGATCG 1 cut(s) 281
PspEI GGTNACC 1 cut(s) 430
PspFI CCCAGC 1 cut(s) 148
PspN4I GGNNCC 3 cut(s) 186, 254, 513
PsrI GAACNNNNNNTAC 2 cut(s) 177, 209
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 2 cut(s) 406, 511
PsyI GACNNNGTC 1 cut(s) 104
PvuI CGATCG 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 152
RruI TCGCGA 1 cut(s) 283
RsaI GTAC 3 cut(s) 176, 186, 311
RsaNI GTAC 3 cut(s) 175, 185, 310
SalI GTCGAC 2 cut(s) 107, 206
SaqAI TTAA 2 cut(s) 96, 222
SatI GCNGC 3 cut(s) 153, 156, 159
Sau3AI GATC 9 cut(s) 4, 66, 118, 274, 278, 292, 406, 511, 521
SfcI CTRYAG 1 cut(s) 153
Sse9I AATT 3 cut(s) 30, 142, 166
SsiI CCGC 1 cut(s) 159
SspMI CTAG 1 cut(s) 20
StyI CCWWGG 1 cut(s) 515
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 2 cut(s) 387, 561
TaqI TCGA 5 cut(s) 69, 108, 135, 207, 277
TasI AATT 3 cut(s) 30, 142, 166
TatI WGTACW 1 cut(s) 174
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 2 cut(s) 96, 222
Tru9I TTAA 2 cut(s) 96, 222
TscAI CASTG 1 cut(s) 217
TseI GCWGC 2 cut(s) 152, 155
TspDTI ATGAA 4 cut(s) 71, 155, 380, 501
TspRI CASTG 1 cut(s) 217
Tth111I GACNNNGTC 1 cut(s) 104
XagI CCTNNNNNAGG 1 cut(s) 504
XapI RAATTY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 489
XmiI GTMKAC 2 cut(s) 108, 207
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.