Rh1DG451400

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004548
Physical Location & Seq
Reverse (-)
65910049 .. 65911363
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG451400.1

Sequence Viewer

Length: 636 bp
ATGCATGCATTCATACATATCATTCACCAGTGTCTCTCAGAAGTCAGAAGCTTAATCGATCTAATGGATCAACTGAGCAACCTAGCTGTACTAATTTGTCATCGTCTCATTCTATTCTTGAAGAAGATGATCGTATACTCATTGTTCAAACCTGAAGTTGAAAACAAGGTCGACACTAACGATCATCATCAGGTCGAGATGAATTTCTCACCTGCAGCCGCTCCGAATTGGTTGCACTTGGAGGTACCAGTAGTTCCGGTGGTAGTTGACACTGAATCGGTTAAGCAACTTCCGGTTGTGCAATACCGGAGTCGAATGATGGAGAGAATAGATCAATCGCGATATAGAGATCACGAGGATGACGAGTCTATGTGTATAGTGTGCATGAATAGCATAGAGGCTATGGATGAAGTAAGAGAGCTGAAGAACTGTTCCCACGTCTTTCATAGAGAGTGCTTGGATCTTTGGGTTGCTCAGGGTCAGGTAACCTGTCCTCTGTGCAGGTCTAAGCTATTGCCAACTGCTAGGGCTACTCCAAATGAACATTTGAAACCTGCGGGAGGGGACCCATGGAGATCGGAGAGGATGATTTACTTGTTCGGTGATGATTGCTTCATGCACTGGATAGACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

211

Amino Acids

24.6

Weight (kDa)

5.59

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 123 - 168 2.1e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 124 - 168 1.4e-11 Ring finger domain
zf-C3HC4_2 PF13923 124 - 167 5.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 125 - 167 2.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 125 - 153 1e-07 RING-like zinc finger
zf-RING_UBOX PF13445 125 - 165 3.2e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 220
Acc36I ACCTGC 3 cut(s) 220, 492, 562
Acc65I GGTACC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 221
AccI GTMKAC 2 cut(s) 135, 171
AccII CGCG 1 cut(s) 340
AciI CCGC 2 cut(s) 219, 557
AclWI GGATC 2 cut(s) 75, 468
AcsI RAATTY 1 cut(s) 202
AcuI CTGAAG 2 cut(s) 174, 443
AfaI GTAC 2 cut(s) 90, 246
AfiI CCNNNNNNNGG 1 cut(s) 560
AgsI TTSAA 4 cut(s) 121, 148, 161, 550
AjiI CACGTC 1 cut(s) 439
AluBI AGCT 4 cut(s) 51, 86, 421, 511
AluI AGCT 4 cut(s) 51, 86, 421, 511
Alw26I GTCTC 2 cut(s) 38, 110
AlwI GGATC 2 cut(s) 75, 468
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 1 cut(s) 202
Asp718I GGTACC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 565
AsuHPI GGTGA 3 cut(s) 17, 201, 614
AvaII GGWCC 1 cut(s) 565
BanI GGYRCC 1 cut(s) 244
BauI CACGAG 1 cut(s) 353
BbvI GCAGC 1 cut(s) 227
BccI CCATC 1 cut(s) 313
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BcoDI GTCTC 2 cut(s) 38, 110
BfaI CTAG 2 cut(s) 83, 525
BfmI CTRYAG 1 cut(s) 213
BfuAI ACCTGC 3 cut(s) 220, 492, 562
BisI GCNGC 2 cut(s) 216, 219
BlsI GCNGC 2 cut(s) 217, 220
Bme18I GGWCC 1 cut(s) 565
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 1 cut(s) 565
BmiI GGNNCC 3 cut(s) 246, 566, 567
Bpu10I CCTNAGC 1 cut(s) 474
Bsa29I ATCGAT 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 569
BsaWI WCCGGW 3 cut(s) 256, 292, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse1I ACTGG 3 cut(s) 28, 248, 626
Bse8I GATNNNNATC 1 cut(s) 186
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 569
BseGI GGATG 3 cut(s) 364, 412, 591
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 1 cut(s) 560
BseMII CTCAG 3 cut(s) 51, 65, 488
BseNI ACTGG 3 cut(s) 28, 248, 626
BseXI GCAGC 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 520
Bsh1236I CGCG 1 cut(s) 340
BshNI GGYRCC 1 cut(s) 244
BshVI ATCGAT 1 cut(s) 57
BsiSI CCGG 3 cut(s) 257, 293, 307
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 2 cut(s) 38, 110
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 8
Bsp143I GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
Bsp19I CCATGG 1 cut(s) 569
Bsp68I TCGCGA 1 cut(s) 340
BspACI CCGC 2 cut(s) 219, 557
BspCNI CTCAG 3 cut(s) 50, 66, 487
BspDI ATCGAT 1 cut(s) 57
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 3 cut(s) 246, 566, 567
BspMAI CTGCAG 1 cut(s) 217
BspMI ACCTGC 3 cut(s) 220, 492, 562
BspPI GGATC 2 cut(s) 75, 468
BspT107I GGYRCC 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 221
BsrI ACTGG 3 cut(s) 28, 248, 626
BssECI CCNNGG 1 cut(s) 569
BssMI GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
BssNAI GTATAC 1 cut(s) 136
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 1 cut(s) 569
Bst1107I GTATAC 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 4 cut(s) 37, 74, 474, 507
BstDSI CCRYGG 1 cut(s) 569
BstEII GGTNACC 1 cut(s) 484
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 3 cut(s) 364, 412, 591
BstFNI CGCG 1 cut(s) 340
BstKTI GATC 8 cut(s) 61, 70, 132, 184, 334, 352, 463, 578
BstMAI GTCTC 2 cut(s) 38, 110
BstMBI GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
BstMWI GCNNNNNNNGC 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BstZ17I GTATAC 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 57
BsuTUI ATCGAT 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 569
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 3 cut(s) 364, 412, 591
BtsIMutI CAGTG 3 cut(s) 35, 270, 619
BtuMI TCGCGA 1 cut(s) 340
BveI ACCTGC 3 cut(s) 220, 492, 562
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 565
ClaI ATCGAT 1 cut(s) 57
Csp6I GTAC 2 cut(s) 89, 245
CviAII CATG 4 cut(s) 5, 385, 570, 616
CviJI RGCY 7 cut(s) 51, 86, 218, 401, 421, 511, 530
CviKI_1 RGCY 7 cut(s) 51, 86, 218, 401, 421, 511, 530
CviQI GTAC 2 cut(s) 89, 245
DdeI CTNAG 4 cut(s) 37, 74, 474, 507
DpnI GATC 8 cut(s) 60, 69, 131, 183, 333, 351, 462, 577
DpnII GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
Eco130I CCWWGG 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 565
Eco57I CTGAAG 2 cut(s) 174, 443
Eco91I GGTNACC 1 cut(s) 484
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoO109I RGGNCCY 1 cut(s) 565
EcoO65I GGTNACC 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 569
EcoT22I ATGCAT 2 cut(s) 6, 10
ErhI CCWWGG 1 cut(s) 569
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 4 cut(s) 8, 388, 573, 619
FaqI GGGAC 1 cut(s) 578
FatI CATG 4 cut(s) 4, 384, 569, 615
FauI CCCGC 1 cut(s) 550
FblI GTMKAC 2 cut(s) 135, 171
Fnu4HI GCNGC 2 cut(s) 216, 219
FokI GGATG 3 cut(s) 371, 419, 598
Fsp4HI GCNGC 2 cut(s) 216, 219
FspBI CTAG 2 cut(s) 83, 525
GluI GCNGC 2 cut(s) 216, 219
HapII CCGG 3 cut(s) 257, 293, 307
Hin1II CATG 4 cut(s) 8, 388, 573, 619
HincII GTYRAC 2 cut(s) 172, 268
HindII GTYRAC 2 cut(s) 172, 268
HindIII AAGCTT 1 cut(s) 49
HinfI GANTC 3 cut(s) 275, 310, 365
HpaII CCGG 3 cut(s) 257, 293, 307
HphI GGTGA 3 cut(s) 17, 201, 614
Hpy166II GTNNAC 3 cut(s) 136, 172, 268
Hpy188I TCNGA 4 cut(s) 40, 47, 225, 580
Hpy188III TCNNGA 4 cut(s) 118, 196, 339, 353
Hpy8I GTNNAC 3 cut(s) 136, 172, 268
HpyCH4III ACNGT 1 cut(s) 431
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 8 cut(s) 4, 8, 215, 235, 301, 384, 501, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 390
HpyF3I CTNAG 4 cut(s) 37, 74, 474, 507
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 4 cut(s) 8, 388, 573, 619
KflI GGGWCCC 1 cut(s) 565
KpnI GGTACC 1 cut(s) 248
Kzo9I GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 1 cut(s) 227
MaeI CTAG 2 cut(s) 83, 525
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 1 cut(s) 484
MalI GATC 8 cut(s) 60, 69, 131, 183, 333, 351, 462, 577
MbiI CCGCTC 1 cut(s) 221
MboI GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
MboII GAAGA 3 cut(s) 133, 136, 436
MflI RGATCY 1 cut(s) 460
MluCI AATT 3 cut(s) 93, 202, 226
MlyI GAGTC 2 cut(s) 319, 374
MnlI CCTC 6 cut(s) 235, 349, 391, 504, 554, 576
Mph1103I ATGCAT 2 cut(s) 6, 10
MseI TTAA 2 cut(s) 53, 282
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 3 cut(s) 257, 293, 307
Mva1269I GAATGC 1 cut(s) 8
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 390
NcoI CCATGG 1 cut(s) 569
NdeII GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
NlaIII CATG 4 cut(s) 8, 388, 573, 619
NlaIV GGNNCC 3 cut(s) 246, 566, 567
NruI TCGCGA 1 cut(s) 340
NsiI ATGCAT 2 cut(s) 6, 10
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 220
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 360
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 2 cut(s) 217, 220
PleI GAGTC 2 cut(s) 318, 373
PpsI GAGTC 2 cut(s) 318, 373
PpuMI RGGWCCY 1 cut(s) 565
Psp5II RGGWCCY 1 cut(s) 565
PspEI GGTNACC 1 cut(s) 484
PspN4I GGNNCC 3 cut(s) 246, 566, 567
PspPI GGNCC 1 cut(s) 565
PspPPI RGGWCCY 1 cut(s) 565
PsrI GAACNNNNNNTAC 2 cut(s) 237, 269
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 460
RruI TCGCGA 1 cut(s) 340
RsaI GTAC 2 cut(s) 90, 246
RsaNI GTAC 2 cut(s) 89, 245
RseI CAYNNNNRTG 1 cut(s) 357
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 53, 282
SatI GCNGC 2 cut(s) 216, 219
Sau3AI GATC 8 cut(s) 58, 67, 129, 181, 331, 349, 460, 575
Sau96I GGNCC 1 cut(s) 565
SchI GAGTC 2 cut(s) 319, 374
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 1 cut(s) 565
SmiMI CAYNNNNRTG 1 cut(s) 357
SphI GCATGC 1 cut(s) 8
Sse9I AATT 3 cut(s) 93, 202, 226
SsiI CCGC 2 cut(s) 219, 557
SspMI CTAG 2 cut(s) 83, 525
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 1 cut(s) 431
TaiI ACGT 1 cut(s) 441
TaqI TCGA 4 cut(s) 57, 171, 195, 313
TasI AATT 3 cut(s) 93, 202, 226
TatI WGTACW 1 cut(s) 88
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 53, 282
Tru9I TTAA 2 cut(s) 53, 282
TscAI CASTG 3 cut(s) 35, 277, 626
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 6 cut(s) 215, 401, 423, 434, 555, 604
TspRI CASTG 3 cut(s) 35, 277, 626
VpaK11BI GGWCC 1 cut(s) 565
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 1 cut(s) 202
XceI RCATGY 1 cut(s) 8
XmiI GTMKAC 2 cut(s) 135, 171
XspI CTAG 2 cut(s) 83, 525
Zsp2I ATGCAT 2 cut(s) 6, 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.