Rh1AG462600

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004548
Physical Location & Seq
Reverse (-)
67887464 .. 67888051
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG462600.1

Sequence Viewer

Length: 588 bp
ATGAAGTTGATGTTGTTGCTGTATGATCTTCTCAAACTTCTACTCGACAAGATCATCTACTATCCAGAATTATTCCTTAGATACAGTGAAGATTATCAACAAAGTAAGGACGATAGTGATCATCAGCTGGAGGTCATGAACTTTGAAGCTTCAGCAACTTCAGATCCTACTTGGTTGAACATGCAAGTACCAGTGGTTCCGGTGGTAATTGACACAGAATTGATTAAGAAGCAACTACCGGTTGTGCTATACCGGGATCTAATTCTGAAGAAAACAAGCCGCCAACGCCAATTTCAAGCTACATCATCATCTTCGGAGCAGGAAATGCACATGTCATCATGTATTGTGTGCATGAATAGCATGGAGGGGAGGGACGAGGTGAGAGAGCTGTGCAATTGTGAGCATGTTTTCCACAGAGACTGCCTGGATGCTTGGATCGGTCAGCCTCAGCTAACTTGTCCTCTTTGTAGATCTGAGCTATTAATACCAGCTCCAACTTCAAAGCATGATAAAGATGGAGGAAGAGATCTATGGAGGACCCAGAGGATGATTTACTTGTTTGGTGAAGATATTTTCTTCAATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

195

Amino Acids

22.87

Weight (kDa)

5.11

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 110 - 157 9.7e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 113 - 157 2e-10 Ring finger domain
zf-RING_11 PF17123 114 - 142 8.4e-06 RING-like zinc finger
zf-C3HC4 PF00097 114 - 156 9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 114 - 154 9.1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 280
AclWI GGATC 3 cut(s) 158, 264, 443
AcuI CTGAAG 3 cut(s) 135, 144, 287
AfaI GTAC 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 330
AgeI ACCGGT 1 cut(s) 238
AgsI TTSAA 5 cut(s) 146, 178, 296, 501, 580
AjnI CCWGG 1 cut(s) 423
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 7 cut(s) 127, 149, 299, 388, 451, 478, 491
AluI AGCT 7 cut(s) 127, 149, 299, 388, 451, 478, 491
Alw26I GTCTC 1 cut(s) 411
AlwI GGATC 3 cut(s) 158, 264, 443
AlwNI CAGNNNCTG 1 cut(s) 420
AseI ATTAAT 1 cut(s) 482
AsiGI ACCGGT 1 cut(s) 238
AspS9I GGNCC 1 cut(s) 537
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 391, 575
AvaII GGWCC 1 cut(s) 537
BbvCI CCTCAGC 1 cut(s) 447
BccI CCATC 1 cut(s) 509
BciT130I CCWGG 1 cut(s) 425
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 1 cut(s) 586
BglII AGATCT 2 cut(s) 470, 526
BisI GCNGC 1 cut(s) 280
BlsI GCNGC 1 cut(s) 281
Bme1390I CCNGG 2 cut(s) 254, 425
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 2 cut(s) 198, 539
BmrFI CCNGG 2 cut(s) 254, 425
BmsI GCATC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 117
BsaWI WCCGGW 2 cut(s) 199, 238
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 1 cut(s) 191
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 1 cut(s) 425
BseGI GGATG 2 cut(s) 433, 552
BseJI GATNNNNATC 1 cut(s) 117
BseMII CTCAG 2 cut(s) 461, 465
BseNI ACTGG 1 cut(s) 191
BshTI ACCGGT 1 cut(s) 238
BsiSI CCGG 3 cut(s) 200, 239, 253
BslFI GGGAC 1 cut(s) 386
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 1 cut(s) 386
Bsp143I GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
BspACI CCGC 1 cut(s) 280
BspCNI CTCAG 2 cut(s) 460, 466
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 198, 539
BspPI GGATC 3 cut(s) 158, 264, 443
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 238
BssMI GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 517
BstAPI GCANNNNNTGC 1 cut(s) 325
BstDEI CTNAG 3 cut(s) 77, 447, 474
BstF5I GGATG 2 cut(s) 433, 552
BstKTI GATC 8 cut(s) 28, 54, 121, 166, 259, 438, 473, 529
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
BstMWI GCNNNNNNNGC 3 cut(s) 285, 325, 357
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 3 cut(s) 184, 334, 407
BstSCI CCNGG 2 cut(s) 252, 423
BstX2I RGATCY 4 cut(s) 163, 256, 470, 526
BstYI RGATCY 4 cut(s) 163, 256, 470, 526
BtsCI GGATG 2 cut(s) 433, 552
BtsIMutI CAGTG 2 cut(s) 91, 198
CaiI CAGNNNCTG 1 cut(s) 420
CciI TCATGA 1 cut(s) 135
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 1 cut(s) 188
CspAI ACCGGT 1 cut(s) 238
CviAII CATG 8 cut(s) 136, 181, 331, 339, 352, 361, 404, 506
CviJI RGCY 9 cut(s) 127, 149, 279, 299, 388, 445, 451, 478, 491
CviKI_1 RGCY 9 cut(s) 127, 149, 279, 299, 388, 445, 451, 478, 491
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 3 cut(s) 77, 447, 474
DpnI GATC 8 cut(s) 27, 53, 120, 165, 258, 437, 472, 528
DpnII GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
Eam1104I CTCTTC 1 cut(s) 517
EarI CTCTTC 1 cut(s) 517
Eco47I GGWCC 1 cut(s) 537
Eco57I CTGAAG 3 cut(s) 135, 144, 287
EcoO109I RGGNCCY 1 cut(s) 537
EcoRII CCWGG 1 cut(s) 423
FaeI CATG 8 cut(s) 139, 184, 334, 342, 355, 364, 407, 509
FaqI GGGAC 1 cut(s) 386
FatI CATG 8 cut(s) 135, 180, 330, 338, 351, 360, 403, 505
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 280
FokI GGATG 2 cut(s) 440, 559
Fsp4HI GCNGC 1 cut(s) 280
FspBI CTAG 1 cut(s) 586
GluI GCNGC 1 cut(s) 280
GsuI CTGGAG 1 cut(s) 149
HapII CCGG 3 cut(s) 200, 239, 253
Hin1II CATG 8 cut(s) 139, 184, 334, 342, 355, 364, 407, 509
HindIII AAGCTT 1 cut(s) 147
HpaII CCGG 3 cut(s) 200, 239, 253
HphI GGTGA 2 cut(s) 391, 575
Hpy188I TCNGA 4 cut(s) 163, 267, 316, 475
Hpy188III TCNNGA 2 cut(s) 65, 136
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 4 cut(s) 184, 328, 351, 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 285, 325, 357
HpyF3I CTNAG 3 cut(s) 77, 447, 474
Hsp92II CATG 8 cut(s) 139, 184, 334, 342, 355, 364, 407, 509
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
LmnI GCTCC 2 cut(s) 316, 496
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 586
MalI GATC 8 cut(s) 27, 53, 120, 165, 258, 437, 472, 528
MboI GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
MboII GAAGA 7 cut(s) 20, 101, 280, 303, 534, 568, 578
MfeI CAATTG 1 cut(s) 394
MflI RGATCY 4 cut(s) 163, 256, 470, 526
MluCI AATT 6 cut(s) 68, 207, 218, 261, 290, 394
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 9 cut(s) 124, 358, 363, 370, 456, 471, 512, 528, 537
MseI TTAA 2 cut(s) 225, 482
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 3 cut(s) 200, 239, 253
MspR9I CCNGG 2 cut(s) 254, 425
MunI CAATTG 1 cut(s) 394
MvaI CCWGG 1 cut(s) 425
MwoI GCNNNNNNNGC 3 cut(s) 285, 325, 357
NciI CCSGG 1 cut(s) 254
NdeII GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
NlaIII CATG 8 cut(s) 139, 184, 334, 342, 355, 364, 407, 509
NlaIV GGNNCC 2 cut(s) 198, 539
NspI RCATGY 3 cut(s) 184, 334, 407
PagI TCATGA 1 cut(s) 135
PciI ACATGT 1 cut(s) 330
PinAI ACCGGT 1 cut(s) 238
PkrI GCNGC 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 537
PscI ACATGT 1 cut(s) 330
PshBI ATTAAT 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 2 cut(s) 198, 539
PspPI GGNCC 1 cut(s) 537
PspPPI RGGWCCY 1 cut(s) 537
PsrI GAACNNNNNNTAC 2 cut(s) 180, 212
PstNI CAGNNNCTG 1 cut(s) 420
PsuI RGATCY 4 cut(s) 163, 256, 470, 526
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 225, 482
SatI GCNGC 1 cut(s) 280
Sau3AI GATC 8 cut(s) 25, 51, 118, 163, 256, 435, 470, 526
Sau96I GGNCC 1 cut(s) 537
ScrFI CCNGG 2 cut(s) 254, 425
SetI ASST 9 cut(s) 129, 135, 151, 301, 381, 390, 453, 480, 493
SfaNI GCATC 1 cut(s) 418
SinI GGWCC 1 cut(s) 537
Sse9I AATT 6 cut(s) 68, 207, 218, 261, 290, 394
SsiI CCGC 1 cut(s) 280
SspMI CTAG 1 cut(s) 586
StyD4I CCNGG 2 cut(s) 252, 423
TaaI ACNGT 1 cut(s) 86
TaqI TCGA 1 cut(s) 45
TaqII GACCGA 1 cut(s) 428
TasI AATT 6 cut(s) 68, 207, 218, 261, 290, 394
TauI GCSGC 1 cut(s) 282
Tru1I TTAA 2 cut(s) 225, 482
Tru9I TTAA 2 cut(s) 225, 482
TscAI CASTG 2 cut(s) 91, 198
TspDTI ATGAA 3 cut(s) 17, 152, 368
TspRI CASTG 2 cut(s) 91, 198
VpaK11BI GGWCC 1 cut(s) 537
VspI ATTAAT 1 cut(s) 482
XceI RCATGY 3 cut(s) 184, 334, 407
XspI CTAG 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.