Rh1AG462300

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004548
Physical Location & Seq
Reverse (-)
67878885 .. 67879496
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG462300.1

Sequence Viewer

Length: 612 bp
ATGCTCAAGGTGAAGTTGATGTTGTTGCTGTGTGATCTTCTCAAACTTCTGCTCGAGAAGATCATCTACTATCCAGAATCATTCCTTAGACACAGTGAAGATGATCAACAAAGTAAGGACGATAGTGATCATCAGCTGGAGGTCTTGAACTTTGAAGATGCAGCAACTTCAGATCCTACTTGGTTGCACATGCAAGTACCAGTGGTTCCGGTGGTAAATATTGACACAGAATTCATTAAGAAGCAACTACCGGTTGTGCTATACCGGGACCTAATTCTGAAGAAAACAAGCAGCCAACGCCAATTTCAAGCTACATCATCATCTTCGGAGCAGGAAATGCACATGTCATCATGTATTGTGTGCATGAATAGCATGGAGGGCAGGGACGAGGTGAGAGAGCTGTGCAATTGTGAGCATGTTTTCCACAGAGACTGCCTGGATGCTTGGATCGGTCAGGCTCAGCTAACTTGTCCTCTTTGTAGATCTGAGCTACTAATACCCGCTCCTACTTCTGCTACTACTTCAAAGCATGATAAAGATGGAGGAAGAGATCCATGGAGGGCCGAGAGGATGATTTACTTGTTTGGTGAAGATATTTTTTTCACGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

203

Amino Acids

23.45

Weight (kDa)

5.03

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 114 - 161 8.3e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 117 - 161 5.2e-11 Ring finger domain
zf-C3HC4 PF00097 118 - 160 3.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 118 - 160 6e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 118 - 158 5.8e-06 RING-type zinc-finger
zf-RING_11 PF17123 118 - 146 9e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 503
AciI CCGC 1 cut(s) 501
AclWI GGATC 3 cut(s) 167, 455, 545
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 2 cut(s) 153, 299
AfaI GTAC 1 cut(s) 198
AflIII ACRYGT 1 cut(s) 342
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 4 cut(s) 148, 155, 308, 525
AjnI CCWGG 1 cut(s) 435
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 5 cut(s) 136, 311, 400, 463, 490
AluI AGCT 5 cut(s) 136, 311, 400, 463, 490
Alw26I GTCTC 1 cut(s) 423
AlwI GGATC 3 cut(s) 167, 455, 545
AlwNI CAGNNNCTG 1 cut(s) 432
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 1 cut(s) 561
ApeKI GCWGC 2 cut(s) 161, 291
ApoI RAATTY 1 cut(s) 230
AsiGI ACCGGT 1 cut(s) 250
AspS9I GGNCC 2 cut(s) 268, 561
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 22, 403, 599
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 268
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbvI GCAGC 2 cut(s) 173, 303
BccI CCATC 1 cut(s) 533
BciT130I CCWGG 1 cut(s) 437
BclI TGATCA 2 cut(s) 103, 127
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 1 cut(s) 610
BglII AGATCT 1 cut(s) 482
BisI GCNGC 2 cut(s) 162, 292
BlpI GCTNAGC 1 cut(s) 459
BlsI GCNGC 2 cut(s) 163, 293
Bme1390I CCNGG 2 cut(s) 266, 437
Bme18I GGWCC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 268, 561
BmiI GGNNCC 2 cut(s) 207, 269
BmrFI CCNGG 2 cut(s) 266, 437
BmsI GCATC 2 cut(s) 148, 430
BpmI CTGGAG 1 cut(s) 158
Bpu1102I GCTNAGC 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 554
BsaWI WCCGGW 2 cut(s) 208, 250
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 200
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 1 cut(s) 554
BseGI GGATG 2 cut(s) 445, 576
BseJI GATNNNNATC 1 cut(s) 126
BseMII CTCAG 2 cut(s) 473, 477
BseNI ACTGG 1 cut(s) 200
BseXI GCAGC 2 cut(s) 173, 303
BshFI GGCC 1 cut(s) 563
BshTI ACCGGT 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 3 cut(s) 209, 251, 265
BslFI GGGAC 2 cut(s) 281, 398
BsmAI GTCTC 1 cut(s) 423
BsmFI GGGAC 2 cut(s) 281, 398
BsnI GGCC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 53
Bsp143I GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
Bsp1720I GCTNAGC 1 cut(s) 459
Bsp19I CCATGG 1 cut(s) 554
BspACI CCGC 1 cut(s) 501
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 2 cut(s) 472, 478
BspLI GGNNCC 2 cut(s) 207, 269
BspPI GGATC 3 cut(s) 167, 455, 545
BsrBI CCGCTC 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 554
BssMI GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
BssT1I CCWWGG 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 95
Bst6I CTCTTC 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 337
BstDEI CTNAG 3 cut(s) 86, 459, 486
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 445, 576
BstKTI GATC 9 cut(s) 37, 63, 106, 130, 175, 450, 485, 553, 609
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
BstMWI GCNNNNNNNGC 4 cut(s) 297, 337, 369, 378
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 3 cut(s) 193, 346, 419
BstSCI CCNGG 2 cut(s) 264, 435
BstV1I GCAGC 2 cut(s) 173, 303
BstX2I RGATCY 3 cut(s) 172, 482, 550
BstYI RGATCY 3 cut(s) 172, 482, 550
BsuRI GGCC 1 cut(s) 563
BtgI CCRYGG 1 cut(s) 554
BtsCI GGATG 2 cut(s) 445, 576
BtsIMutI CAGTG 2 cut(s) 100, 207
CaiI CAGNNNCTG 1 cut(s) 432
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 268, 561
Csp6I GTAC 1 cut(s) 197
CspAI ACCGGT 1 cut(s) 250
CviAII CATG 8 cut(s) 190, 343, 351, 364, 373, 416, 530, 555
CviJI RGCY 8 cut(s) 136, 294, 311, 400, 458, 463, 490, 563
CviKI_1 RGCY 8 cut(s) 136, 294, 311, 400, 458, 463, 490, 563
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 3 cut(s) 86, 459, 486
DpnI GATC 9 cut(s) 36, 62, 105, 129, 174, 449, 484, 552, 608
DpnII GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
Eam1104I CTCTTC 1 cut(s) 541
EarI CTCTTC 1 cut(s) 541
Eco130I CCWWGG 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 153, 299
Eco88I CYCGRG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 554
FaeI CATG 8 cut(s) 193, 346, 354, 367, 376, 419, 533, 558
FaiI YATR 9 cut(s) 191, 262, 344, 352, 365, 374, 417, 531, 556
FaqI GGGAC 2 cut(s) 281, 398
FatI CATG 8 cut(s) 189, 342, 350, 363, 372, 415, 529, 554
FauI CCCGC 1 cut(s) 508
FbaI TGATCA 2 cut(s) 103, 127
Fnu4HI GCNGC 2 cut(s) 162, 292
FokI GGATG 2 cut(s) 452, 583
Fsp4HI GCNGC 2 cut(s) 162, 292
FspBI CTAG 1 cut(s) 610
GluI GCNGC 2 cut(s) 162, 292
GsuI CTGGAG 1 cut(s) 158
HaeIII GGCC 1 cut(s) 563
HapII CCGG 3 cut(s) 209, 251, 265
Hin1II CATG 8 cut(s) 193, 346, 354, 367, 376, 419, 533, 558
HinfI GANTC 1 cut(s) 77
HpaII CCGG 3 cut(s) 209, 251, 265
HphI GGTGA 3 cut(s) 22, 403, 599
Hpy188I TCNGA 4 cut(s) 172, 279, 328, 487
Hpy188III TCNNGA 4 cut(s) 55, 74, 145, 604
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4V TGCA 6 cut(s) 161, 187, 193, 340, 363, 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 297, 337, 369, 378
HpyF3I CTNAG 3 cut(s) 86, 459, 486
Hsp92II CATG 8 cut(s) 193, 346, 354, 367, 376, 419, 533, 558
Ksp22I TGATCA 2 cut(s) 103, 127
Kzo9I GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
LmnI GCTCC 2 cut(s) 328, 508
Lsp1109I GCAGC 2 cut(s) 173, 303
LweI GCATC 2 cut(s) 148, 430
MaeI CTAG 1 cut(s) 610
MalI GATC 9 cut(s) 36, 62, 105, 129, 174, 449, 484, 552, 608
MbiI CCGCTC 1 cut(s) 503
MboI GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
MboII GAAGA 8 cut(s) 29, 70, 110, 167, 292, 315, 558, 602
MfeI CAATTG 1 cut(s) 406
MflI RGATCY 3 cut(s) 172, 482, 550
MluCI AATT 4 cut(s) 230, 273, 302, 406
MnlI CCTC 7 cut(s) 133, 370, 382, 483, 536, 552, 561
MseI TTAA 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 3 cut(s) 209, 251, 265
MspR9I CCNGG 2 cut(s) 266, 437
MunI CAATTG 1 cut(s) 406
MvaI CCWGG 1 cut(s) 437
MwoI GCNNNNNNNGC 4 cut(s) 297, 337, 369, 378
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 554
NdeII GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
NlaIII CATG 8 cut(s) 193, 346, 354, 367, 376, 419, 533, 558
NlaIV GGNNCC 2 cut(s) 207, 269
NmeAIII GCCGAG 1 cut(s) 589
NspI RCATGY 3 cut(s) 193, 346, 419
PaeR7I CTCGAG 1 cut(s) 53
PciI ACATGT 1 cut(s) 342
PfeI GAWTC 1 cut(s) 77
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 2 cut(s) 163, 293
PpuMI RGGWCCY 1 cut(s) 268
PscI ACATGT 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 207, 269
PspPI GGNCC 2 cut(s) 268, 561
PspPPI RGGWCCY 1 cut(s) 268
PsrI GAACNNNNNNTAC 2 cut(s) 189, 221
PstNI CAGNNNCTG 1 cut(s) 432
PsuI RGATCY 3 cut(s) 172, 482, 550
PvuII CAGCTG 1 cut(s) 136
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 2 cut(s) 162, 292
Sau3AI GATC 9 cut(s) 34, 60, 103, 127, 172, 447, 482, 550, 606
Sau96I GGNCC 2 cut(s) 268, 561
ScrFI CCNGG 2 cut(s) 266, 437
SetI ASST 9 cut(s) 12, 138, 144, 273, 313, 393, 402, 465, 492
SfaNI GCATC 2 cut(s) 148, 430
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 53
SmlI CTYRAG 2 cut(s) 5, 53
SmoI CTYRAG 2 cut(s) 5, 53
Sse9I AATT 4 cut(s) 230, 273, 302, 406
SsiI CCGC 1 cut(s) 501
SspI AATATT 1 cut(s) 220
SspMI CTAG 1 cut(s) 610
StyD4I CCNGG 2 cut(s) 264, 435
StyI CCWWGG 1 cut(s) 554
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 1 cut(s) 54
TaqII GACCGA 1 cut(s) 440
TasI AATT 4 cut(s) 230, 273, 302, 406
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 2 cut(s) 100, 207
TseI GCWGC 2 cut(s) 161, 291
TspDTI ATGAA 2 cut(s) 223, 380
TspRI CASTG 2 cut(s) 100, 207
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 1 cut(s) 230
XceI RCATGY 3 cut(s) 193, 346, 419
XhoI CTCGAG 1 cut(s) 53
XspI CTAG 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.