Rh1BG419400

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004548
Physical Location & Seq
Reverse (-)
54013897 .. 54014556
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG419400.1

Sequence Viewer

Length: 660 bp
ATGCGTGCTTCTTTCTGTAATTGTGATATAATCCAGCGAATAAGAAGCATGCTCAAGATGAAGTTGATGTTGTTGCTGTGTGATCTTCTCAAACTTCTGCTCGAGAAGATCATCTACTATCCAGAATCATTCCTCAGACACAGTGAAGATGATCAACAAAGTAAGGAAGATAGTGATCATCAGCTGGAGGTCATGAACTTTGAAGCTGCAGCAACTTCAGATCCTACTTGGTTGCACATGCAAGTACCAGTGGTTCCGGTGGTAAATATTGACACAGAATTCATTAAGAAGCAACTACCGGTTGTGCTATACCGGGACCTAATTCTGAAGAAAACAAGCTGCCAACGCCAATTTCAAGCTACATCATCATTTTCGGAGCAGGAAATGCACATGTCATCATGTATTGTGTGCATGAATAGCATGGAGGGCAGGGACGAGGTGAGAGAGCTGTGCAATTGTGAGCATGTTTTCCACAGAGACTGCCTGGATGCTTGGATCGGTCAGGCTCAGCTAACTTGTCCTCTTTGTAGATCTGAGCTACTAATACCCGCTTCTACTTCTGCTACTACTTCAAAGCATGATAAAGATGGAGGAAGAGATCCATGGAGGGCCGAGAGGATGATTTACTTGTTTGGTGAAGATATTTGCTTCACGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

219

Amino Acids

25.39

Weight (kDa)

5.38

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 130 - 177 6.7e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 133 - 177 5.9e-11 Ring finger domain
zf-C3HC4 PF00097 134 - 176 4.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 134 - 176 6.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 134 - 162 1e-05 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 549
AclWI GGATC 3 cut(s) 215, 503, 593
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 2 cut(s) 201, 347
AfaI GTAC 1 cut(s) 246
AflIII ACRYGT 1 cut(s) 390
AgeI ACCGGT 1 cut(s) 298
AgsI TTSAA 3 cut(s) 203, 356, 573
AjnI CCWGG 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 336, 368
AluBI AGCT 7 cut(s) 184, 206, 339, 359, 448, 511, 538
AluI AGCT 7 cut(s) 184, 206, 339, 359, 448, 511, 538
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 3 cut(s) 215, 503, 593
AlwNI CAGNNNCTG 1 cut(s) 480
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 1 cut(s) 609
ApeKI GCWGC 3 cut(s) 206, 209, 339
ApoI RAATTY 1 cut(s) 278
AsiGI ACCGGT 1 cut(s) 298
AspS9I GGNCC 2 cut(s) 316, 609
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 2 cut(s) 451, 647
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 1 cut(s) 316
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BbvI GCAGC 3 cut(s) 193, 221, 326
BccI CCATC 1 cut(s) 581
BciT130I CCWGG 1 cut(s) 485
BclI TGATCA 2 cut(s) 151, 175
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 1 cut(s) 471
BfaI CTAG 1 cut(s) 658
BfmI CTRYAG 1 cut(s) 207
BglII AGATCT 1 cut(s) 530
BisI GCNGC 3 cut(s) 207, 210, 340
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 3 cut(s) 208, 211, 341
Bme1390I CCNGG 2 cut(s) 314, 485
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 316, 609
BmiI GGNNCC 2 cut(s) 255, 317
BmrFI CCNGG 2 cut(s) 314, 485
BmsI GCATC 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 602
BsaWI WCCGGW 2 cut(s) 256, 298
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 248
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 602
BseGI GGATG 2 cut(s) 493, 624
BseJI GATNNNNATC 1 cut(s) 174
BseMII CTCAG 3 cut(s) 148, 521, 525
BseNI ACTGG 1 cut(s) 248
BseXI GCAGC 3 cut(s) 193, 221, 326
BshFI GGCC 1 cut(s) 611
BshTI ACCGGT 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 3 cut(s) 257, 299, 313
BslFI GGGAC 2 cut(s) 329, 446
BsmAI GTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 329, 446
BsnI GGCC 1 cut(s) 611
BsoBI CYCGRG 1 cut(s) 101
Bsp143I GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
Bsp1720I GCTNAGC 1 cut(s) 507
Bsp19I CCATGG 1 cut(s) 602
BspACI CCGC 1 cut(s) 549
BspANI GGCC 1 cut(s) 611
BspCNI CTCAG 3 cut(s) 147, 520, 526
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 255, 317
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 3 cut(s) 215, 503, 593
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 602
BssMI GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
BssT1I CCWWGG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 589
BstAPI GCANNNNNTGC 1 cut(s) 385
BstC8I GCNNGC 2 cut(s) 6, 50
BstDEI CTNAG 3 cut(s) 134, 507, 534
BstDSI CCRYGG 1 cut(s) 602
BstF5I GGATG 2 cut(s) 493, 624
BstKTI GATC 9 cut(s) 85, 111, 154, 178, 223, 498, 533, 601, 657
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
BstMWI GCNNNNNNNGC 4 cut(s) 345, 385, 417, 426
BstNI CCWGG 1 cut(s) 485
BstNSI RCATGY 4 cut(s) 52, 241, 394, 467
BstSCI CCNGG 2 cut(s) 312, 483
BstSFI CTRYAG 1 cut(s) 207
BstV1I GCAGC 3 cut(s) 193, 221, 326
BstX2I RGATCY 3 cut(s) 220, 530, 598
BstYI RGATCY 3 cut(s) 220, 530, 598
BsuRI GGCC 1 cut(s) 611
BtgI CCRYGG 1 cut(s) 602
BtsCI GGATG 2 cut(s) 493, 624
BtsIMutI CAGTG 2 cut(s) 148, 255
Cac8I GCNNGC 2 cut(s) 6, 50
CaiI CAGNNNCTG 1 cut(s) 480
CciI TCATGA 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 2 cut(s) 316, 609
Csp6I GTAC 1 cut(s) 245
CspAI ACCGGT 1 cut(s) 298
CviJI RGCY 9 cut(s) 184, 206, 339, 359, 448, 506, 511, 538, 611
CviKI_1 RGCY 9 cut(s) 184, 206, 339, 359, 448, 506, 511, 538, 611
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 3 cut(s) 134, 507, 534
DpnI GATC 9 cut(s) 84, 110, 153, 177, 222, 497, 532, 600, 656
DpnII GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
Eam1104I CTCTTC 1 cut(s) 589
EarI CTCTTC 1 cut(s) 589
Eco130I CCWWGG 1 cut(s) 602
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 201, 347
Eco88I CYCGRG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 602
ErhI CCWWGG 1 cut(s) 602
FaqI GGGAC 2 cut(s) 329, 446
FauI CCCGC 1 cut(s) 556
FbaI TGATCA 2 cut(s) 151, 175
Fnu4HI GCNGC 3 cut(s) 207, 210, 340
FokI GGATG 2 cut(s) 500, 631
Fsp4HI GCNGC 3 cut(s) 207, 210, 340
FspBI CTAG 1 cut(s) 658
GluI GCNGC 3 cut(s) 207, 210, 340
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 1 cut(s) 611
HapII CCGG 3 cut(s) 257, 299, 313
HinfI GANTC 1 cut(s) 125
HpaII CCGG 3 cut(s) 257, 299, 313
HphI GGTGA 2 cut(s) 451, 647
Hpy188I TCNGA 5 cut(s) 137, 220, 327, 376, 535
Hpy188III TCNNGA 5 cut(s) 55, 103, 122, 193, 652
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 6 cut(s) 209, 235, 241, 388, 411, 453
HpyF10VI GCNNNNNNNGC 4 cut(s) 345, 385, 417, 426
HpyF3I CTNAG 3 cut(s) 134, 507, 534
Ksp22I TGATCA 2 cut(s) 151, 175
Kzo9I GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 3 cut(s) 193, 221, 326
LweI GCATC 1 cut(s) 478
MaeI CTAG 1 cut(s) 658
MalI GATC 9 cut(s) 84, 110, 153, 177, 222, 497, 532, 600, 656
MboI GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
MboII GAAGA 7 cut(s) 77, 118, 158, 179, 340, 606, 650
MfeI CAATTG 1 cut(s) 454
MflI RGATCY 3 cut(s) 220, 530, 598
MluCI AATT 5 cut(s) 19, 278, 321, 350, 454
MnlI CCTC 8 cut(s) 143, 181, 418, 430, 531, 584, 600, 609
MseI TTAA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 3 cut(s) 257, 299, 313
MspR9I CCNGG 2 cut(s) 314, 485
MunI CAATTG 1 cut(s) 454
MvaI CCWGG 1 cut(s) 485
MwoI GCNNNNNNNGC 4 cut(s) 345, 385, 417, 426
NciI CCSGG 1 cut(s) 314
NcoI CCATGG 1 cut(s) 602
NdeII GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
NlaIV GGNNCC 2 cut(s) 255, 317
NmeAIII GCCGAG 1 cut(s) 637
NspI RCATGY 4 cut(s) 52, 241, 394, 467
PaeI GCATGC 1 cut(s) 52
PaeR7I CTCGAG 1 cut(s) 101
PagI TCATGA 1 cut(s) 192
PciI ACATGT 1 cut(s) 390
PfeI GAWTC 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 298
PkrI GCNGC 3 cut(s) 208, 211, 341
PpuMI RGGWCCY 1 cut(s) 316
PscI ACATGT 1 cut(s) 390
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 483
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 2 cut(s) 255, 317
PspPI GGNCC 2 cut(s) 316, 609
PspPPI RGGWCCY 1 cut(s) 316
PsrI GAACNNNNNNTAC 2 cut(s) 237, 269
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 480
PsuI RGATCY 3 cut(s) 220, 530, 598
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 3 cut(s) 207, 210, 340
Sau3AI GATC 9 cut(s) 82, 108, 151, 175, 220, 495, 530, 598, 654
Sau96I GGNCC 2 cut(s) 316, 609
ScrFI CCNGG 2 cut(s) 314, 485
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 1 cut(s) 207
Sfr274I CTCGAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 53, 101
SmoI CTYRAG 2 cut(s) 53, 101
SphI GCATGC 1 cut(s) 52
Sse9I AATT 5 cut(s) 19, 278, 321, 350, 454
SsiI CCGC 1 cut(s) 549
SspI AATATT 1 cut(s) 268
SspMI CTAG 1 cut(s) 658
StyD4I CCNGG 2 cut(s) 312, 483
StyI CCWWGG 1 cut(s) 602
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 102
TaqII GACCGA 1 cut(s) 488
TasI AATT 5 cut(s) 19, 278, 321, 350, 454
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 2 cut(s) 148, 255
TseI GCWGC 3 cut(s) 206, 209, 339
TspDTI ATGAA 4 cut(s) 74, 209, 271, 428
TspRI CASTG 2 cut(s) 148, 255
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 278
XceI RCATGY 4 cut(s) 52, 241, 394, 467
XhoI CTCGAG 1 cut(s) 101
XspI CTAG 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.