MD07G1299500.v1.1

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004548
Physical Location & Seq
Reverse (-)
35851935 .. 35852165
231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1299500.v1.1.491

Sequence Viewer

Length: 231 bp
ATGGATAGCATGGAGGAAGGCCAAGTGGTGAGAGAGCCATGCAATTGTTCACATGCTTTTCATAAGGAGTGCCTAGATGTCTGGATTGATCAAGGTCAGCTAACATGTCCTCTCTGTAGATCAGAGCTATTGCCTAAAACTTGTAGTACTTCCCCAAACTCAAAAGATAAGGATCCATGGAGGAAGGAGAGGATGATTTACTTGTTTGGTGATGATTACTTCATGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

77

Amino Acids

8.88

Weight (kDa)

4.97

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_2 PF13923 1 - 39 4.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 2 - 40 4.1e-09 Ring finger domain
zf-rbx1 PF12678 14 - 40 7.7e-09 RING-H2 zinc finger domain
zf-C3HC4 PF00097 14 - 39 1.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 15 - 43 9.3e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 167, 180
AfaI GTAC 1 cut(s) 148
AflIII ACRYGT 1 cut(s) 104
AluBI AGCT 3 cut(s) 100, 127, 228
AluI AGCT 3 cut(s) 100, 127, 228
AlwI GGATC 2 cut(s) 167, 180
AoxI GGCC 1 cut(s) 19
AsuHPI GGTGA 2 cut(s) 40, 221
BamHI GGATCC 1 cut(s) 172
BclI TGATCA 1 cut(s) 88
BfaI CTAG 2 cut(s) 74, 229
BfmI CTRYAG 1 cut(s) 115
BmcAI AGTACT 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 176
Bse8I GATNNNNATC 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 1 cut(s) 198
BseJI GATNNNNATC 1 cut(s) 171
BshFI GGCC 1 cut(s) 21
BsnI GGCC 1 cut(s) 21
Bsp143I GATC 3 cut(s) 88, 119, 172
Bsp19I CCATGG 1 cut(s) 176
BspANI GGCC 1 cut(s) 21
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 174
BspPI GGATC 2 cut(s) 167, 180
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 3 cut(s) 88, 119, 172
BssT1I CCWWGG 1 cut(s) 176
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 198
BstKTI GATC 3 cut(s) 91, 122, 175
BstMBI GATC 3 cut(s) 88, 119, 172
BstNSI RCATGY 2 cut(s) 56, 108
BstSFI CTRYAG 1 cut(s) 115
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BsuRI GGCC 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 176
BtsCI GGATG 1 cut(s) 198
CciI TCATGA 1 cut(s) 222
Csp6I GTAC 1 cut(s) 147
CviAII CATG 6 cut(s) 10, 39, 53, 105, 177, 223
CviJI RGCY 5 cut(s) 21, 37, 100, 127, 228
CviKI_1 RGCY 5 cut(s) 21, 37, 100, 127, 228
CviQI GTAC 1 cut(s) 147
DpnI GATC 3 cut(s) 90, 121, 174
DpnII GATC 3 cut(s) 88, 119, 172
Eco130I CCWWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 6 cut(s) 13, 42, 56, 108, 180, 226
FaiI YATR 7 cut(s) 11, 40, 54, 63, 106, 178, 224
FatI CATG 6 cut(s) 9, 38, 52, 104, 176, 222
FbaI TGATCA 1 cut(s) 88
FokI GGATG 1 cut(s) 205
FspBI CTAG 2 cut(s) 74, 229
HaeIII GGCC 1 cut(s) 21
Hin1II CATG 6 cut(s) 13, 42, 56, 108, 180, 226
HphI GGTGA 2 cut(s) 40, 221
Hpy166II GTNNAC 1 cut(s) 50
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 2 cut(s) 82, 223
Hpy8I GTNNAC 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 11, 178
HpyCH4V TGCA 1 cut(s) 42
Hsp92II CATG 6 cut(s) 13, 42, 56, 108, 180, 226
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 3 cut(s) 88, 119, 172
LpnPI CCDG 1 cut(s) 67
MaeI CTAG 2 cut(s) 74, 229
MalI GATC 3 cut(s) 90, 121, 174
MboI GATC 3 cut(s) 88, 119, 172
MfeI CAATTG 1 cut(s) 43
MflI RGATCY 1 cut(s) 172
MluCI AATT 1 cut(s) 43
MnlI CCTC 4 cut(s) 7, 120, 174, 183
MunI CAATTG 1 cut(s) 43
NcoI CCATGG 1 cut(s) 176
NdeII GATC 3 cut(s) 88, 119, 172
NlaIII CATG 6 cut(s) 13, 42, 56, 108, 180, 226
NlaIV GGNNCC 1 cut(s) 174
NspI RCATGY 2 cut(s) 56, 108
PagI TCATGA 1 cut(s) 222
PciI ACATGT 1 cut(s) 104
PscI ACATGT 1 cut(s) 104
PspN4I GGNNCC 1 cut(s) 174
PsuI RGATCY 1 cut(s) 172
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
Sau3AI GATC 3 cut(s) 88, 119, 172
ScaI AGTACT 1 cut(s) 148
SetI ASST 4 cut(s) 97, 102, 129, 230
SfcI CTRYAG 1 cut(s) 115
Sse9I AATT 1 cut(s) 43
SspMI CTAG 2 cut(s) 74, 229
StyI CCWWGG 1 cut(s) 176
TasI AATT 1 cut(s) 43
TatI WGTACW 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 50, 211
XceI RCATGY 2 cut(s) 56, 108
XspI CTAG 2 cut(s) 74, 229
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.