RLG00000026149

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004548
Physical Location & Seq
Forward (+)
843787 .. 844407
621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026149

Sequence Viewer

Length: 621 bp
ATGAAGTTGATGTTGTTGCTGTGTGATCTTCTCAAACTTCTGCTCGAGAAGATCATCTACTATCCAGAATCATTCCTTAGACACAGTGAAGATGATCATCAACAAAGTAAGGACGATAGTGATCATCAGCTGGAGGTCATGAACTTTGAAGCTGCAGCAACTTCAGATCCTACTTGGTTGCACATGCAAGTACCAGTGGTTCCGGTGGTAAATATTGACACAGAATTCATTAAGAAGCAACTACCGGTTGTGCTATACCAGGACCTAATTATTCTGAAGAAAACAAGTCGCCAACGCCAATTTCAAGCTACATTATCAGCGTTATCATCATCTTCGGAGCAGGAAATGCACATGTCATCATGTATTGTGTGCATGAATAGCATCGAGGGCAGGGACGAGGTGAGAGAGCTGTCCAACTGTGAGCATGTTTTCCACAGACACTGCCTGGATGCTTGGATCGGTCAGGCTCGGCTAACTTGTCCTCTTTGTAGATCTGAGCTACTAATACCCGCTTCTACTTCTGCTACTACTTCAAAGCATGACAAAGATGGAGGAAGAGATCCATGGAGGGCCGAGAGGATGATTTACTTGTTTGGTGAAGATATTTTCTTCACGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

207

Amino Acids

23.77

Weight (kDa)

5.43

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 118 - 164 4.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 120 - 164 2e-11 Ring finger domain
zf-RING_11 PF17123 121 - 149 7.2e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 121 - 163 1.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 121 - 163 2.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 137 - 167 4.9e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 510
AclWI GGATC 3 cut(s) 161, 464, 554
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 2 cut(s) 147, 296
AfaI GTAC 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 3 cut(s) 149, 305, 534
AjnI CCWGG 2 cut(s) 258, 444
AjuI GAANNNNNNNTTGG 2 cut(s) 285, 317
AluBI AGCT 5 cut(s) 130, 152, 308, 409, 499
AluI AGCT 5 cut(s) 130, 152, 308, 409, 499
AlwI GGATC 3 cut(s) 161, 464, 554
AlwNI CAGNNNCTG 1 cut(s) 441
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 570
ApeKI GCWGC 2 cut(s) 152, 155
ApoI RAATTY 1 cut(s) 224
AsiGI ACCGGT 1 cut(s) 244
AspS9I GGNCC 2 cut(s) 262, 570
AsuHPI GGTGA 2 cut(s) 412, 608
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 262
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbvI GCAGC 2 cut(s) 139, 167
BccI CCATC 1 cut(s) 542
BciT130I CCWGG 2 cut(s) 260, 446
BclI TGATCA 2 cut(s) 94, 121
BfaI CTAG 1 cut(s) 619
BfmI CTRYAG 1 cut(s) 153
BglII AGATCT 1 cut(s) 491
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
Bme1390I CCNGG 2 cut(s) 260, 446
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 262, 570
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 260, 446
BmsI GCATC 2 cut(s) 390, 439
BpmI CTGGAG 1 cut(s) 152
BsaBI GATNNNNATC 2 cut(s) 96, 120
BsaJI CCNNGG 1 cut(s) 563
BsaWI WCCGGW 2 cut(s) 202, 244
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 194
Bse8I GATNNNNATC 2 cut(s) 96, 120
BseBI CCWGG 2 cut(s) 260, 446
BseDI CCNNGG 1 cut(s) 563
BseGI GGATG 2 cut(s) 454, 585
BseJI GATNNNNATC 2 cut(s) 96, 120
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 1 cut(s) 194
BseXI GCAGC 2 cut(s) 139, 167
BshFI GGCC 1 cut(s) 572
BshTI ACCGGT 1 cut(s) 244
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 2 cut(s) 203, 245
BslFI GGGAC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 1 cut(s) 572
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
Bsp19I CCATGG 1 cut(s) 563
BspACI CCGC 1 cut(s) 510
BspANI GGCC 1 cut(s) 572
BspCNI CTCAG 1 cut(s) 487
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 3 cut(s) 161, 464, 554
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 1 cut(s) 563
BssMI GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
BssT1I CCWWGG 1 cut(s) 563
Bst2UI CCWGG 2 cut(s) 260, 446
Bst4CI ACNGT 2 cut(s) 86, 419
Bst6I CTCTTC 1 cut(s) 550
BstAPI GCANNNNNTGC 1 cut(s) 346
BstDEI CTNAG 2 cut(s) 77, 495
BstDSI CCRYGG 1 cut(s) 563
BstF5I GGATG 2 cut(s) 454, 585
BstKTI GATC 9 cut(s) 28, 54, 97, 124, 169, 459, 494, 562, 618
BstMBI GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
BstMWI GCNNNNNNNGC 3 cut(s) 346, 378, 387
BstNI CCWGG 2 cut(s) 260, 446
BstNSI RCATGY 3 cut(s) 187, 355, 428
BstSCI CCNGG 2 cut(s) 258, 444
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 139, 167
BstX2I RGATCY 3 cut(s) 166, 491, 559
BstYI RGATCY 3 cut(s) 166, 491, 559
BsuRI GGCC 1 cut(s) 572
BtgI CCRYGG 1 cut(s) 563
BtsCI GGATG 2 cut(s) 454, 585
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 3 cut(s) 91, 201, 439
CaiI CAGNNNCTG 1 cut(s) 441
CciI TCATGA 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 262, 570
Csp6I GTAC 1 cut(s) 191
CspAI ACCGGT 1 cut(s) 244
CviAII CATG 8 cut(s) 139, 184, 352, 360, 373, 425, 539, 564
CviJI RGCY 8 cut(s) 130, 152, 308, 409, 467, 472, 499, 572
CviKI_1 RGCY 8 cut(s) 130, 152, 308, 409, 467, 472, 499, 572
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 2 cut(s) 77, 495
DpnI GATC 9 cut(s) 27, 53, 96, 123, 168, 458, 493, 561, 617
DpnII GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
Eam1104I CTCTTC 1 cut(s) 550
EarI CTCTTC 1 cut(s) 550
Eco130I CCWWGG 1 cut(s) 563
Eco47I GGWCC 1 cut(s) 262
Eco57I CTGAAG 2 cut(s) 147, 296
Eco88I CYCGRG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 258, 444
EcoT14I CCWWGG 1 cut(s) 563
ErhI CCWWGG 1 cut(s) 563
FaeI CATG 8 cut(s) 142, 187, 355, 363, 376, 428, 542, 567
FaiI YATR 9 cut(s) 140, 185, 256, 353, 361, 374, 426, 540, 565
FaqI GGGAC 1 cut(s) 407
FatI CATG 8 cut(s) 138, 183, 351, 359, 372, 424, 538, 563
FauI CCCGC 1 cut(s) 517
FbaI TGATCA 2 cut(s) 94, 121
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 2 cut(s) 461, 592
Fsp4HI GCNGC 2 cut(s) 153, 156
FspBI CTAG 1 cut(s) 619
GluI GCNGC 2 cut(s) 153, 156
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 572
HapII CCGG 2 cut(s) 203, 245
Hin1II CATG 8 cut(s) 142, 187, 355, 363, 376, 428, 542, 567
HinfI GANTC 1 cut(s) 68
HpaII CCGG 2 cut(s) 203, 245
HphI GGTGA 2 cut(s) 412, 608
Hpy188I TCNGA 4 cut(s) 166, 276, 337, 496
Hpy188III TCNNGA 4 cut(s) 46, 65, 139, 613
HpyCH4III ACNGT 2 cut(s) 86, 419
HpyCH4V TGCA 5 cut(s) 155, 181, 187, 349, 372
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 378, 387
HpyF3I CTNAG 2 cut(s) 77, 495
Hsp92II CATG 8 cut(s) 142, 187, 355, 363, 376, 428, 542, 567
Ksp22I TGATCA 2 cut(s) 94, 121
Kzo9I GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 2 cut(s) 139, 167
LweI GCATC 2 cut(s) 390, 439
MaeI CTAG 1 cut(s) 619
MalI GATC 9 cut(s) 27, 53, 96, 123, 168, 458, 493, 561, 617
MboI GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
MboII GAAGA 8 cut(s) 20, 61, 101, 289, 324, 567, 601, 611
MflI RGATCY 3 cut(s) 166, 491, 559
MluCI AATT 3 cut(s) 224, 267, 299
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 7 cut(s) 127, 379, 391, 492, 545, 561, 570
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 130
MspI CCGG 2 cut(s) 203, 245
MspR9I CCNGG 2 cut(s) 260, 446
MvaI CCWGG 2 cut(s) 260, 446
MwoI GCNNNNNNNGC 3 cut(s) 346, 378, 387
NcoI CCATGG 1 cut(s) 563
NdeII GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
NlaIII CATG 8 cut(s) 142, 187, 355, 363, 376, 428, 542, 567
NlaIV GGNNCC 1 cut(s) 201
NmeAIII GCCGAG 2 cut(s) 448, 598
NspI RCATGY 3 cut(s) 187, 355, 428
PaeR7I CTCGAG 1 cut(s) 44
PagI TCATGA 1 cut(s) 138
PciI ACATGT 1 cut(s) 351
PfeI GAWTC 1 cut(s) 68
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 2 cut(s) 154, 157
PpuMI RGGWCCY 1 cut(s) 262
PscI ACATGT 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 2 cut(s) 258, 444
PspGI CCWGG 2 cut(s) 258, 444
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 2 cut(s) 262, 570
PspPPI RGGWCCY 1 cut(s) 262
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PstI CTGCAG 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 441
PsuI RGATCY 3 cut(s) 166, 491, 559
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 153, 156
Sau3AI GATC 9 cut(s) 25, 51, 94, 121, 166, 456, 491, 559, 615
Sau96I GGNCC 2 cut(s) 262, 570
ScrFI CCNGG 2 cut(s) 260, 446
SetI ASST 8 cut(s) 132, 138, 154, 267, 310, 402, 411, 501
SfaNI GCATC 2 cut(s) 390, 439
SfcI CTRYAG 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 1 cut(s) 262
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 3 cut(s) 224, 267, 299
SsiI CCGC 1 cut(s) 510
SspI AATATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 619
StyD4I CCNGG 2 cut(s) 258, 444
StyI CCWWGG 1 cut(s) 563
TaaI ACNGT 2 cut(s) 86, 419
TaqI TCGA 2 cut(s) 45, 384
TaqII GACCGA 1 cut(s) 449
TasI AATT 3 cut(s) 224, 267, 299
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 3 cut(s) 91, 201, 446
TseI GCWGC 2 cut(s) 152, 155
TspDTI ATGAA 4 cut(s) 17, 155, 217, 389
TspRI CASTG 3 cut(s) 91, 201, 446
VpaK11BI GGWCC 1 cut(s) 262
XapI RAATTY 1 cut(s) 224
XceI RCATGY 3 cut(s) 187, 355, 428
XhoI CTCGAG 1 cut(s) 44
XspI CTAG 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.