RchiOBHm_Chr1g0382921

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004548
Physical Location & Seq
Reverse (-)
67506391 .. 67507207
817 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60586

Sequence Viewer

Length: 672 bp
ATGCGTGCTTCTTTCTGTAATTGTGATATAATCCAGCGAATAAGAAGCATGCTCAAGATGAAGTTGATGTTGTTGCTGTGTGATCTTCTCAAACTTCTGCTCGAGAAGATCATCTACTATCCAGAATCATTCCTTAGACACAGTGAAGATGATCAACAAAGTAAGGACGATAGTGATCATCAGCTGGAGGTCATGAACTTTGAAGTTGCAGCAACTTCAGATCCTACTAGGTTGCACATGCAAGTACCAGTGGTTCCGGTGGTAAATATTGACACAGAATTCATTAAGAAGCAACTACCGGTTGTGCTATACCGGGACCTAATTCTGAAGAAAACAAGCGGCCAACGCCAATTTCAAGCTACATCATCAGCGTTATCATCATTTTCGGAGCAGGAAATGCACATGTCATCATGTATTGTGTGCATGAATAGCATGGAGGGCAGGGACGAGGTGAGAGAGCTGTGCAATTGTGAGCATGTTTTCCATAGAGACTGCCTGGATGCTTGGATCGGTCAGGCTCAGCTAACTTGTCCTCTTTGTAGATCTGAGCTACTAATACCTGCTTCTACTTCTGCTACTACTTCAAAGCATGACAACGATGGAGGAAGAGACCCATGGAGGGCCGAGAGGATGATTTACTTGTTTGGTGAAGATATTTGCTTCACGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

223

Amino Acids

25.67

Weight (kDa)

5.37

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 134 - 181 1.1e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 137 - 181 6.1e-11 Ring finger domain
zf-C3HC4 PF00097 138 - 180 4.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 138 - 180 7e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 138 - 166 1e-05 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 568
AciI CCGC 1 cut(s) 339
AclWI GGATC 2 cut(s) 215, 515
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 2 cut(s) 201, 347
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 1 cut(s) 619
AflIII ACRYGT 1 cut(s) 402
AgeI ACCGGT 1 cut(s) 298
AgsI TTSAA 3 cut(s) 203, 356, 585
AjnI CCWGG 1 cut(s) 495
AjuI GAANNNNNNNTTGG 2 cut(s) 336, 368
AluBI AGCT 5 cut(s) 184, 359, 460, 523, 550
AluI AGCT 5 cut(s) 184, 359, 460, 523, 550
Alw26I GTCTC 2 cut(s) 483, 603
AlwI GGATC 2 cut(s) 215, 515
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 2 cut(s) 340, 621
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 1 cut(s) 278
AsiGI ACCGGT 1 cut(s) 298
AspS9I GGNCC 2 cut(s) 316, 621
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 2 cut(s) 463, 659
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 1 cut(s) 316
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BbvI GCAGC 1 cut(s) 221
BccI CCATC 1 cut(s) 593
BciT130I CCWGG 1 cut(s) 497
BclI TGATCA 2 cut(s) 151, 175
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 2 cut(s) 483, 603
BfaI CTAG 2 cut(s) 228, 670
BfuAI ACCTGC 1 cut(s) 568
BglII AGATCT 1 cut(s) 542
BisI GCNGC 2 cut(s) 210, 340
BlpI GCTNAGC 1 cut(s) 519
BlsI GCNGC 2 cut(s) 211, 341
Bme1390I CCNGG 2 cut(s) 314, 497
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 316, 621
BmiI GGNNCC 2 cut(s) 255, 317
BmrFI CCNGG 2 cut(s) 314, 497
BmsI GCATC 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 2 cut(s) 256, 298
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 248
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 2 cut(s) 505, 636
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMII CTCAG 2 cut(s) 533, 537
BseNI ACTGG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 221
BshFI GGCC 2 cut(s) 342, 623
BshTI ACCGGT 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 3 cut(s) 257, 299, 313
BslFI GGGAC 2 cut(s) 329, 458
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 2 cut(s) 483, 603
BsmFI GGGAC 2 cut(s) 329, 458
BsnI GGCC 2 cut(s) 342, 623
Bso31I GGTCTC 1 cut(s) 603
BsoBI CYCGRG 1 cut(s) 101
Bsp143I GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
Bsp1720I GCTNAGC 1 cut(s) 519
Bsp19I CCATGG 1 cut(s) 614
BspACI CCGC 1 cut(s) 339
BspANI GGCC 2 cut(s) 342, 623
BspCNI CTCAG 2 cut(s) 532, 538
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 255, 317
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 2 cut(s) 215, 515
BspTNI GGTCTC 1 cut(s) 603
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
BssT1I CCWWGG 1 cut(s) 614
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 601
BstAPI GCANNNNNTGC 1 cut(s) 397
BstC8I GCNNGC 2 cut(s) 6, 50
BstDEI CTNAG 3 cut(s) 134, 519, 546
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 2 cut(s) 505, 636
BstKTI GATC 8 cut(s) 85, 111, 154, 178, 223, 510, 545, 669
BstMAI GTCTC 2 cut(s) 483, 603
BstMBI GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
BstMWI GCNNNNNNNGC 4 cut(s) 345, 397, 429, 438
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 4 cut(s) 52, 241, 406, 479
BstSCI CCNGG 2 cut(s) 312, 495
BstV1I GCAGC 1 cut(s) 221
BstX2I RGATCY 2 cut(s) 220, 542
BstYI RGATCY 2 cut(s) 220, 542
BsuRI GGCC 2 cut(s) 342, 623
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 2 cut(s) 505, 636
BtsIMutI CAGTG 2 cut(s) 148, 255
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 2 cut(s) 6, 50
CciI TCATGA 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 2 cut(s) 316, 621
Csp6I GTAC 1 cut(s) 245
CspAI ACCGGT 1 cut(s) 298
CviJI RGCY 8 cut(s) 184, 342, 359, 460, 518, 523, 550, 623
CviKI_1 RGCY 8 cut(s) 184, 342, 359, 460, 518, 523, 550, 623
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 3 cut(s) 134, 519, 546
DpnI GATC 8 cut(s) 84, 110, 153, 177, 222, 509, 544, 668
DpnII GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 601
EarI CTCTTC 1 cut(s) 601
Eco130I CCWWGG 1 cut(s) 614
Eco31I GGTCTC 1 cut(s) 603
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 201, 347
Eco88I CYCGRG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 614
FaqI GGGAC 2 cut(s) 329, 458
FbaI TGATCA 2 cut(s) 151, 175
Fnu4HI GCNGC 2 cut(s) 210, 340
FokI GGATG 2 cut(s) 512, 643
Fsp4HI GCNGC 2 cut(s) 210, 340
FspBI CTAG 2 cut(s) 228, 670
GluI GCNGC 2 cut(s) 210, 340
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 2 cut(s) 342, 623
HapII CCGG 3 cut(s) 257, 299, 313
HinfI GANTC 1 cut(s) 125
HpaII CCGG 3 cut(s) 257, 299, 313
HphI GGTGA 2 cut(s) 463, 659
Hpy188I TCNGA 4 cut(s) 220, 327, 388, 547
Hpy188III TCNNGA 5 cut(s) 55, 103, 122, 193, 664
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 6 cut(s) 209, 235, 241, 400, 423, 465
HpyF10VI GCNNNNNNNGC 4 cut(s) 345, 397, 429, 438
HpyF3I CTNAG 3 cut(s) 134, 519, 546
Ksp22I TGATCA 2 cut(s) 151, 175
Kzo9I GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 1 cut(s) 490
MaeI CTAG 2 cut(s) 228, 670
MalI GATC 8 cut(s) 84, 110, 153, 177, 222, 509, 544, 668
MboI GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
MboII GAAGA 6 cut(s) 77, 118, 158, 340, 618, 662
MfeI CAATTG 1 cut(s) 466
MflI RGATCY 2 cut(s) 220, 542
MluCI AATT 5 cut(s) 19, 278, 321, 350, 466
MnlI CCTC 7 cut(s) 181, 430, 442, 543, 596, 612, 621
MseI TTAA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 3 cut(s) 257, 299, 313
MspR9I CCNGG 2 cut(s) 314, 497
MunI CAATTG 1 cut(s) 466
MvaI CCWGG 1 cut(s) 497
MwoI GCNNNNNNNGC 4 cut(s) 345, 397, 429, 438
NciI CCSGG 1 cut(s) 314
NcoI CCATGG 1 cut(s) 614
NdeII GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
NlaIV GGNNCC 2 cut(s) 255, 317
NmeAIII GCCGAG 1 cut(s) 649
NspI RCATGY 4 cut(s) 52, 241, 406, 479
PaeI GCATGC 1 cut(s) 52
PaeR7I CTCGAG 1 cut(s) 101
PagI TCATGA 1 cut(s) 192
PciI ACATGT 1 cut(s) 402
PfeI GAWTC 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 298
PkrI GCNGC 2 cut(s) 211, 341
PpuMI RGGWCCY 1 cut(s) 316
PscI ACATGT 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 2 cut(s) 255, 317
PspPI GGNCC 2 cut(s) 316, 621
PspPPI RGGWCCY 1 cut(s) 316
PsrI GAACNNNNNNTAC 2 cut(s) 237, 269
PsuI RGATCY 2 cut(s) 220, 542
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 2 cut(s) 210, 340
Sau3AI GATC 8 cut(s) 82, 108, 151, 175, 220, 507, 542, 666
Sau96I GGNCC 2 cut(s) 316, 621
ScrFI CCNGG 2 cut(s) 314, 497
SfaNI GCATC 1 cut(s) 490
Sfr274I CTCGAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 53, 101
SmoI CTYRAG 2 cut(s) 53, 101
SphI GCATGC 1 cut(s) 52
Sse9I AATT 5 cut(s) 19, 278, 321, 350, 466
SsiI CCGC 1 cut(s) 339
SspI AATATT 1 cut(s) 268
SspMI CTAG 2 cut(s) 228, 670
StyD4I CCNGG 2 cut(s) 312, 495
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 102
TaqII GACCGA 1 cut(s) 500
TasI AATT 5 cut(s) 19, 278, 321, 350, 466
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 2 cut(s) 148, 255
TseI GCWGC 1 cut(s) 209
TspDTI ATGAA 4 cut(s) 74, 209, 271, 440
TspRI CASTG 2 cut(s) 148, 255
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 278
XceI RCATGY 4 cut(s) 52, 241, 406, 479
XhoI CTCGAG 1 cut(s) 101
XspI CTAG 2 cut(s) 228, 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.