MD02G1056800.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001178
Physical Location & Seq
Reverse (-)
4600010 .. 4605008
4999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1056800.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGAATAGAGGTGGTTGCCAGAGCTCACCGGTGCAACAAATGATGGCCGGAAACCCTAATTGGTGGAGTATCAACAGCATGAGGCCACCAAGTACCCAACCATCTATGAATATTTCTCCTTTCTTTCATCCCCCTTCTCCTCTTCCTCCTAATTTTTTAATCCCACAATTTGCACCCAACAACAACAACAACACTTCCCCCCCTTCTTCAACAATTTCTTCTTCAGGACTTGCCATTCCTTCTTGGCATATTGAAAATAACAACCAGGAGCTTCCAGAGTCTTGGAGCCAACTTCTCTTGGGTGGATTGGTAGGTGAAGATGAGAAAGGGGTAATCATGAGACAATTTCATCAAGCTGCTAAGAAATTGGAAAATTGGGAGGAGCAAATATTAAATAGCACTCAACCTCCAAATGCTCCTGCTGTGGACGTGAAGCAAGAAAACTCAGGAAGCAGCGGCGGCTTTATGTACGGACATCATCATCACGGGAATGATCACATCCAAGATTTTAACCCATCAGCTGCAGCTTTGTCTTCTTTGTCTCAGATTATGCAATCAACTACTCCATCTGCGGCCTCATCTCCTAAGTCATCGTGTGTGACGAGTTTCAGCAACAACAATATGTTGGATTTTTCTAGCAAGTCGGACGTGAGGCATCCACCACCACATCGATCCTCTGATCAGTGTAACAATGGTGGGGCGCTTAAGAGAGCTAGGGTTCAACCTTCTTCAACCCCTACTACCTTCAAGGTGAGGAAGGAGAAATTAGGTGACAGAATTACAGCCCTTCATCAGCTAGTTTCTCCATTTGGGAAGACTGACACAGCCTCTGTCTTGTTAGAAGCTATTGGGTACATCAGATTCCTTCAGAGTCAAATTGAGGCCCTCAGTTTACCTTACTTGGGCAGTGGATCAGGAAACATGCTGCAGCAGCCACAGTCTGTAGTTCAAGGGGAGAGGAATTGTATGTTCCCTGAAGATCCTGGTCAGCTGCTCAATGACAATTGCATGAAGAGGAAAGGAGCTTCTGAGCAGGATCCTCATGAAGAGCCAAAAAAGGACCTGAGGAGTAGGGGCTTGTGTTTGGTTCCAGTTTCATGTACCCTCCAAGTTGGGAGCGATAATGGAGCTGACTATTGGGCTCCGGCGCTCAGCGGCGGAGGTTTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

391

Amino Acids

42.32

Weight (kDa)

6.45

Isoelectric Point (pI)

66.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 456, 459, 572, 1155, 1158
AclWI GGATC 5 cut(s) 666, 919, 974, 1031, 1044
AcoI YGGCCR 1 cut(s) 45
AcuI CTGAAG 3 cut(s) 207, 851, 996
AfaI GTAC 4 cut(s) 94, 470, 854, 1102
AfiI CCNNNNNNNGG 1 cut(s) 902
AflII CTTAAG 1 cut(s) 704
AgeI ACCGGT 1 cut(s) 28
AgsI TTSAA 6 cut(s) 210, 254, 722, 732, 748, 950
AjiI CACGTC 2 cut(s) 430, 649
AjnI CCWGG 2 cut(s) 264, 982
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 2 cut(s) 334, 546
AlwI GGATC 5 cut(s) 666, 919, 974, 1031, 1044
AlwNI CAGNNNCTG 1 cut(s) 830
AoxI GGCC 4 cut(s) 45, 83, 573, 882
ApeKI GCWGC 8 cut(s) 356, 453, 521, 524, 925, 928, 931, 991
AsiGI ACCGGT 1 cut(s) 28
AspLEI GCGC 2 cut(s) 703, 1150
AspS9I GGNCC 2 cut(s) 883, 1060
AsuHPI GGTGA 4 cut(s) 18, 326, 763, 782
AvaII GGWCC 1 cut(s) 1060
AxyI CCTNAGG 1 cut(s) 1064
BamHI GGATCC 1 cut(s) 1036
BanII GRGCYC 2 cut(s) 26, 1144
BbsI GAAGAC 2 cut(s) 525, 821
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 8 cut(s) 343, 465, 508, 536, 912, 940, 943, 978
BccI CCATC 4 cut(s) 37, 109, 523, 574
BciT130I CCWGG 2 cut(s) 266, 984
BclI TGATCA 2 cut(s) 493, 679
BcoDI GTCTC 2 cut(s) 334, 546
BfaI CTAG 3 cut(s) 636, 714, 797
BfmI CTRYAG 3 cut(s) 522, 926, 942
BfoI RGCGCY 2 cut(s) 704, 1151
BfrI CTTAAG 1 cut(s) 704
BlpI GCTNAGC 1 cut(s) 1151
Bme1390I CCNGG 2 cut(s) 266, 984
Bme18I GGWCC 1 cut(s) 1060
BmgBI CACGTC 2 cut(s) 430, 649
BmgT120I GGNCC 2 cut(s) 883, 1060
BmiI GGNNCC 4 cut(s) 287, 1038, 1089, 1143
BmrFI CCNGG 2 cut(s) 266, 984
BmsI GCATC 1 cut(s) 664
BpiI GAAGAC 2 cut(s) 525, 821
Bpu1102I GCTNAGC 1 cut(s) 1151
Bsa29I ATCGAT 1 cut(s) 670
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 902
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 1 cut(s) 1091
Bse21I CCTNAGG 1 cut(s) 1064
BseBI CCWGG 2 cut(s) 266, 984
BseCI ATCGAT 1 cut(s) 670
BseGI GGATG 3 cut(s) 127, 498, 655
BseLI CCNNNNNNNGG 1 cut(s) 902
BseMII CTCAG 6 cut(s) 459, 557, 901, 1020, 1055, 1165
BseNI ACTGG 1 cut(s) 1091
BseRI GAGGAG 3 cut(s) 129, 395, 1081
BseXI GCAGC 8 cut(s) 343, 465, 508, 536, 912, 940, 943, 978
BshFI GGCC 4 cut(s) 47, 85, 575, 884
BshTI ACCGGT 1 cut(s) 28
BshVI ATCGAT 1 cut(s) 670
BsiHKAI GWGCWC 1 cut(s) 26
BsiSI CCGG 3 cut(s) 29, 48, 1145
BslI CCNNNNNNNGG 1 cut(s) 902
BsmAI GTCTC 2 cut(s) 334, 546
BsnI GGCC 4 cut(s) 47, 85, 575, 884
Bsp1286I GDGCHC 2 cut(s) 26, 1144
Bsp143I GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
Bsp1720I GCTNAGC 1 cut(s) 1151
BspACI CCGC 5 cut(s) 456, 459, 572, 1155, 1158
BspANI GGCC 4 cut(s) 47, 85, 575, 884
BspCNI CTCAG 6 cut(s) 458, 556, 900, 1021, 1056, 1164
BspDI ATCGAT 1 cut(s) 670
BspHI TCATGA 2 cut(s) 336, 1042
BspLI GGNNCC 4 cut(s) 287, 1038, 1089, 1143
BspMAI CTGCAG 2 cut(s) 526, 930
BspPI GGATC 5 cut(s) 666, 919, 974, 1031, 1044
BspQI GCTCTTC 1 cut(s) 1041
BspTI CTTAAG 1 cut(s) 704
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 1 cut(s) 1091
BssAI RCCGGY 1 cut(s) 28
BssMI GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
Bst2UI CCWGG 2 cut(s) 266, 984
Bst4CI ACNGT 1 cut(s) 939
Bst6I CTCTTC 3 cut(s) 147, 1007, 1041
BstAFI CTTAAG 1 cut(s) 704
BstDEI CTNAG 8 cut(s) 360, 445, 543, 585, 887, 1029, 1064, 1151
BstF5I GGATG 3 cut(s) 127, 498, 655
BstH2I RGCGCY 2 cut(s) 704, 1151
BstHHI GCGC 2 cut(s) 703, 1150
BstKTI GATC 6 cut(s) 496, 674, 682, 914, 982, 1039
BstMAI GTCTC 2 cut(s) 334, 546
BstMBI GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
BstMWI GCNNNNNNNGC 2 cut(s) 459, 931
BstNI CCWGG 2 cut(s) 266, 984
BstNSI RCATGY 1 cut(s) 925
BstSCI CCNGG 2 cut(s) 264, 982
BstSFI CTRYAG 3 cut(s) 522, 926, 942
BstV1I GCAGC 8 cut(s) 343, 465, 508, 536, 912, 940, 943, 978
BstV2I GAAGAC 2 cut(s) 525, 821
BstX2I RGATCY 2 cut(s) 979, 1036
BstXI CCANNNNNNTGG 1 cut(s) 282
BstYI RGATCY 2 cut(s) 979, 1036
Bsu15I ATCGAT 1 cut(s) 670
Bsu36I CCTNAGG 1 cut(s) 1064
BsuRI GGCC 4 cut(s) 47, 85, 575, 884
BsuTUI ATCGAT 1 cut(s) 670
BtrI CACGTC 2 cut(s) 430, 649
BtsCI GGATG 3 cut(s) 127, 498, 655
BtsI GCAGTG 1 cut(s) 913
BtsIMutI CAGTG 2 cut(s) 689, 913
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 2 cut(s) 336, 1042
CfoI GCGC 2 cut(s) 703, 1150
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 883, 1060
ClaI ATCGAT 1 cut(s) 670
Csp6I GTAC 4 cut(s) 93, 469, 853, 1101
CspAI ACCGGT 1 cut(s) 28
CviAII CATG 6 cut(s) 79, 337, 922, 1009, 1043, 1098
CviQI GTAC 4 cut(s) 93, 469, 853, 1101
DdeI CTNAG 8 cut(s) 360, 445, 543, 585, 887, 1029, 1064, 1151
DpnI GATC 6 cut(s) 495, 673, 681, 913, 981, 1038
DpnII GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 147, 1007, 1041
EarI CTCTTC 3 cut(s) 147, 1007, 1041
EciI GGCGGA 1 cut(s) 1173
Ecl136II GAGCTC 1 cut(s) 24
Eco24I GRGCYC 2 cut(s) 26, 1144
Eco47I GGWCC 1 cut(s) 1060
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 3 cut(s) 207, 851, 996
Eco81I CCTNAGG 1 cut(s) 1064
EcoICRI GAGCTC 1 cut(s) 24
EcoO109I RGGNCCY 2 cut(s) 883, 1060
EcoRII CCWGG 2 cut(s) 264, 982
EcoT38I GRGCYC 2 cut(s) 26, 1144
FaeI CATG 6 cut(s) 82, 340, 925, 1012, 1046, 1101
FatI CATG 6 cut(s) 78, 336, 921, 1008, 1042, 1097
FbaI TGATCA 2 cut(s) 493, 679
FokI GGATG 3 cut(s) 114, 485, 642
FriOI GRGCYC 2 cut(s) 26, 1144
FspBI CTAG 3 cut(s) 636, 714, 797
GlaI GCGC 2 cut(s) 702, 1149
HaeII RGCGCY 2 cut(s) 704, 1151
HaeIII GGCC 4 cut(s) 47, 85, 575, 884
HapII CCGG 3 cut(s) 29, 48, 1145
HhaI GCGC 2 cut(s) 703, 1150
Hin1II CATG 6 cut(s) 82, 340, 925, 1012, 1046, 1101
Hin6I GCGC 2 cut(s) 701, 1148
HinP1I GCGC 2 cut(s) 701, 1148
HinfI GANTC 3 cut(s) 278, 861, 871
HpaII CCGG 3 cut(s) 29, 48, 1145
HphI GGTGA 4 cut(s) 18, 326, 763, 782
Hpy166II GTNNAC 2 cut(s) 427, 893
Hpy188I TCNGA 6 cut(s) 546, 646, 679, 860, 870, 1030
Hpy188III TCNNGA 6 cut(s) 225, 275, 337, 447, 915, 1043
Hpy8I GTNNAC 2 cut(s) 427, 893
HpyAV CCTTC 8 cut(s) 144, 213, 249, 735, 751, 754, 797, 875
HpyCH4III ACNGT 1 cut(s) 939
HpyCH4IV ACGT 2 cut(s) 429, 648
HpyCH4V TGCA 6 cut(s) 34, 173, 524, 553, 928, 1008
HpyF10VI GCNNNNNNNGC 2 cut(s) 459, 931
HpyF3I CTNAG 8 cut(s) 360, 445, 543, 585, 887, 1029, 1064, 1151
HpySE526I ACGT 2 cut(s) 429, 648
Hsp92II CATG 6 cut(s) 82, 340, 925, 1012, 1046, 1101
HspAI GCGC 2 cut(s) 701, 1148
Ksp22I TGATCA 2 cut(s) 493, 679
Kzo9I GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 8 cut(s) 268, 285, 382, 421, 1022, 1116, 1127, 1147
Lsp1109I GCAGC 8 cut(s) 343, 465, 508, 536, 912, 940, 943, 978
LweI GCATC 1 cut(s) 664
MaeI CTAG 3 cut(s) 636, 714, 797
MaeII ACGT 2 cut(s) 429, 648
MaeIII GTNAC 3 cut(s) 598, 686, 770
MalI GATC 6 cut(s) 495, 673, 681, 913, 981, 1038
MboI GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
MfeI CAATTG 1 cut(s) 1003
MflI RGATCY 2 cut(s) 979, 1036
MhlI GDGCHC 2 cut(s) 26, 1144
MlyI GAGTC 2 cut(s) 287, 880
MmeI TCCRAC 2 cut(s) 606, 624
MseI TTAA 4 cut(s) 158, 392, 510, 705
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 4 cut(s) 456, 521, 991, 1155
MspCI CTTAAG 1 cut(s) 704
MspI CCGG 3 cut(s) 29, 48, 1145
MspR9I CCNGG 2 cut(s) 266, 984
MunI CAATTG 1 cut(s) 1003
MvaI CCWGG 2 cut(s) 266, 984
MwoI GCNNNNNNNGC 2 cut(s) 459, 931
NdeII GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
NlaIII CATG 6 cut(s) 82, 340, 925, 1012, 1046, 1101
NlaIV GGNNCC 4 cut(s) 287, 1038, 1089, 1143
NmuCI GTSAC 2 cut(s) 598, 770
NspI RCATGY 1 cut(s) 925
PagI TCATGA 2 cut(s) 336, 1042
PciSI GCTCTTC 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 1 cut(s) 599
PfeI GAWTC 1 cut(s) 861
PinAI ACCGGT 1 cut(s) 28
PleI GAGTC 2 cut(s) 286, 879
PpsI GAGTC 2 cut(s) 286, 879
PpuMI RGGWCCY 1 cut(s) 1060
Psp124BI GAGCTC 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 1060
Psp6I CCWGG 2 cut(s) 264, 982
PspGI CCWGG 2 cut(s) 264, 982
PspN4I GGNNCC 4 cut(s) 287, 1038, 1089, 1143
PspPI GGNCC 2 cut(s) 883, 1060
PspPPI RGGWCCY 1 cut(s) 1060
PstI CTGCAG 2 cut(s) 526, 930
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 2 cut(s) 979, 1036
PvuII CAGCTG 2 cut(s) 521, 991
RsaI GTAC 4 cut(s) 94, 470, 854, 1102
RsaNI GTAC 4 cut(s) 93, 469, 853, 1101
RseI CAYNNNNRTG 1 cut(s) 489
SacI GAGCTC 1 cut(s) 26
SapI GCTCTTC 1 cut(s) 1041
SaqAI TTAA 4 cut(s) 158, 392, 510, 705
Sau3AI GATC 6 cut(s) 493, 671, 679, 911, 979, 1036
Sau96I GGNCC 2 cut(s) 883, 1060
SchI GAGTC 2 cut(s) 287, 880
ScrFI CCNGG 2 cut(s) 266, 984
SduI GDGCHC 2 cut(s) 26, 1144
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 3 cut(s) 522, 926, 942
SgrAI CRCCGGYG 1 cut(s) 28
SinI GGWCC 1 cut(s) 1060
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SsiI CCGC 5 cut(s) 456, 459, 572, 1155, 1158
SspI AATATT 2 cut(s) 112, 390
SspMI CTAG 3 cut(s) 636, 714, 797
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 264, 982
TaaI ACNGT 1 cut(s) 939
TaiI ACGT 2 cut(s) 432, 651
TaqI TCGA 1 cut(s) 670
TauI GCSGC 4 cut(s) 459, 462, 575, 1158
TfiI GAWTC 1 cut(s) 861
Tru1I TTAA 4 cut(s) 158, 392, 510, 705
Tru9I TTAA 4 cut(s) 158, 392, 510, 705
TscAI CASTG 2 cut(s) 689, 913
TseFI GTSAC 2 cut(s) 598, 770
TseI GCWGC 8 cut(s) 356, 453, 521, 524, 925, 928, 931, 991
Tsp45I GTSAC 2 cut(s) 598, 770
TspDTI ATGAA 8 cut(s) 17, 116, 122, 338, 779, 1025, 1059, 1086
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 2 cut(s) 689, 913
Vha464I CTTAAG 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 1060
XceI RCATGY 1 cut(s) 925
XspI CTAG 3 cut(s) 636, 714, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.