MD09G1208300.v1.1
BHLH Family

Transcription factor bHLH68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001178
Physical Location & Seq
Forward (+)
19985322 .. 19988619
3298 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1208300.v1.1.491

Sequence Viewer

Length: 942 bp
ATGATGGCCGGAAACCCCAAGTGGTGGAGCAGCATGCATCAACCATCTCTCTACCCTTCAGAATATGTGCAGCTTGGATCTTCTAGTTCACTCCCTCTGAATTCTTTGCCTGACTATAACCTAGAGCCTCCACAATCCTGGAACCAGTTACTCTTGGGTGGATTGTCCAGCCAGGAAGATAGCTTTGGTACCCTGAGTCATAATTTTCAACCTAAAAGTAGGTTGAAATATTGGGAGGAGGACCAAATCTTGATTCCACAGTATCCATGGGCTCCTCTTCCTCCTCATGGTGTTGATAATATTATAAAGCAAGAGGTTAGTTCCCAGAACAGCATTAACTTATTGGGCAGTACTCGTCATGGAGACGAAGAATTTCAGCCGCAGGCACCTAGCAGCAGCAGGCCTGTTTGGTCCCCAGTTCAAATCATGCCAGTTTCATCCCCTAGGTCTTGTGTCACTAGTTCAAGTAATACTAATAATATATTGGACTTCTCCTATAACAAAACTGACAATAAGAGGAACCAACATGCAGATCACTCATCTGAGTGTCATAGCACTGCCACTGGTCGGATAACCAAGAAGGCCAGGGTTCAACAATCGCCAAGCCAACCACCTCTCAAGGTCAGGAAGGAGAAGCTAGGTGATAGGATAACAGCCCTTCACCAACTAGTTTCCCCATTTGGGAAGACTGACACTGCTTCAGTCCTGTTAGAAGTCATTGGGTATATCAGATTCCTTCAGGGTCAAATTCAGGCCCTCAGCTCCCCTTACTTGGGCAGTGCCTCAAAAAACATGAGGAACCAACTGTCTGAATCACAAGATCATAAGGCAAAGGATCTGGGAAGTAAGGGGTTGTGCTTGGTTCCTGTGTCATGCACTCAGCATCTTGGGAGTGACAATGGAGCTGATTATTGGGCTCCTGCTTATGGAAATGGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

34.62

Weight (kDa)

7.73

Isoelectric Point (pI)

69.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 188
AccB1I GGYRCC 2 cut(s) 188, 385
AccB7I CCANNNNNTGG 1 cut(s) 24
AciI CCGC 1 cut(s) 380
AclWI GGATC 2 cut(s) 85, 843
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 3 cut(s) 100, 371, 747
AcuI CTGAAG 3 cut(s) 42, 684, 722
AfaI GTAC 2 cut(s) 190, 352
AfiI CCNNNNNNNGG 7 cut(s) 24, 287, 567, 681, 772, 773, 926
AgsI TTSAA 5 cut(s) 209, 226, 422, 465, 593
AhlI ACTAGT 2 cut(s) 458, 667
AjnI CCWGG 3 cut(s) 137, 171, 584
AjuI GAANNNNNNNTTGG 4 cut(s) 168, 200, 237, 269
AluBI AGCT 5 cut(s) 73, 183, 637, 762, 905
AluI AGCT 5 cut(s) 73, 183, 637, 762, 905
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 2 cut(s) 85, 843
AoxI GGCC 4 cut(s) 6, 401, 582, 753
ApeKI GCWGC 4 cut(s) 30, 70, 393, 396
ApoI RAATTY 3 cut(s) 100, 371, 747
Asp700I GAANNNNTTC 1 cut(s) 372
Asp718I GGTACC 1 cut(s) 188
AspA2I CCTAGG 1 cut(s) 443
AspS9I GGNCC 3 cut(s) 241, 411, 754
AsuHPI GGTGA 2 cut(s) 653, 653
AvaII GGWCC 2 cut(s) 241, 411
AvrII CCTAGG 1 cut(s) 443
BanI GGYRCC 2 cut(s) 188, 385
BanII GRGCYC 2 cut(s) 274, 919
BbsI GAAGAC 1 cut(s) 692
BbvCI CCTCAGC 1 cut(s) 758
BbvI GCAGC 4 cut(s) 42, 82, 405, 408
BccI CCATC 1 cut(s) 52
BciT130I CCWGG 3 cut(s) 139, 173, 586
BciVI GTATCC 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 357
BcuI ACTAGT 2 cut(s) 458, 667
BfaI CTAG 7 cut(s) 84, 122, 390, 444, 459, 638, 668
BfuI GTATCC 1 cut(s) 273
BisI GCNGC 5 cut(s) 31, 71, 380, 394, 397
BlnI CCTAGG 1 cut(s) 443
BlsI GCNGC 5 cut(s) 32, 72, 381, 395, 398
BmcAI AGTACT 1 cut(s) 352
Bme1390I CCNGG 3 cut(s) 139, 173, 586
Bme18I GGWCC 2 cut(s) 241, 411
BmgT120I GGNCC 3 cut(s) 241, 411, 754
BmiI GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 864, 918
BmrFI CCNGG 3 cut(s) 139, 173, 586
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 2 cut(s) 46, 892
BmuI ACTGGG 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 692
Bpu10I CCTNAGC 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 266, 443, 585
Bsc4I CCNNNNNNNGG 7 cut(s) 24, 287, 567, 681, 772, 773, 926
Bse1I ACTGG 4 cut(s) 145, 416, 431, 568
BseBI CCWGG 3 cut(s) 139, 173, 586
BseDI CCNNGG 3 cut(s) 266, 443, 585
BseGI GGATG 1 cut(s) 437
BseLI CCNNNNNNNGG 7 cut(s) 24, 287, 567, 681, 772, 773, 926
BseMII CTCAG 4 cut(s) 185, 534, 772, 893
BseNI ACTGG 4 cut(s) 145, 416, 431, 568
BseRI GAGGAG 3 cut(s) 251, 264, 273
BseXI GCAGC 4 cut(s) 42, 82, 405, 408
BsgI GTGCAG 1 cut(s) 89
BshFI GGCC 4 cut(s) 8, 403, 584, 755
BshNI GGYRCC 2 cut(s) 188, 385
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 7 cut(s) 24, 287, 567, 681, 772, 773, 926
BsmAI GTCTC 1 cut(s) 357
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 4 cut(s) 8, 403, 584, 755
Bsp1286I GDGCHC 2 cut(s) 274, 919
Bsp143I GATC 4 cut(s) 77, 532, 820, 835
Bsp19I CCATGG 1 cut(s) 266
BspACI CCGC 1 cut(s) 380
BspANI GGCC 4 cut(s) 8, 403, 584, 755
BspCNI CTCAG 4 cut(s) 186, 535, 771, 892
BspLI GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 864, 918
BspPI GGATC 2 cut(s) 85, 843
BspT107I GGYRCC 2 cut(s) 188, 385
BsrI ACTGG 4 cut(s) 145, 416, 431, 568
BssECI CCNNGG 3 cut(s) 266, 443, 585
BssMI GATC 4 cut(s) 77, 532, 820, 835
BssT1I CCWWGG 2 cut(s) 266, 443
Bst2UI CCWGG 3 cut(s) 139, 173, 586
Bst4CI ACNGT 2 cut(s) 261, 807
Bst6I CTCTTC 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 35, 384, 401
BstDEI CTNAG 4 cut(s) 194, 543, 758, 879
BstDSI CCRYGG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 437
BstKTI GATC 4 cut(s) 80, 535, 823, 838
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 4 cut(s) 77, 532, 820, 835
BstNI CCWGG 3 cut(s) 139, 173, 586
BstNSI RCATGY 2 cut(s) 37, 530
BstSCI CCNGG 3 cut(s) 137, 171, 584
BstV1I GCAGC 4 cut(s) 42, 82, 405, 408
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 2 cut(s) 77, 835
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 2 cut(s) 77, 835
BsuI GTATCC 1 cut(s) 273
BsuRI GGCC 4 cut(s) 8, 403, 584, 755
BtgI CCRYGG 1 cut(s) 266
BtsCI GGATG 1 cut(s) 437
BtsI GCAGTG 3 cut(s) 555, 693, 784
BtsIMutI CAGTG 4 cut(s) 555, 561, 693, 784
Cac8I GCNNGC 3 cut(s) 35, 384, 401
Cfr13I GGNCC 3 cut(s) 241, 411, 754
Csp6I GTAC 2 cut(s) 189, 351
CviAII CATG 8 cut(s) 34, 267, 287, 359, 427, 527, 793, 873
CviQI GTAC 2 cut(s) 189, 351
DdeI CTNAG 4 cut(s) 194, 543, 758, 879
DpnI GATC 4 cut(s) 79, 534, 822, 837
DpnII GATC 4 cut(s) 77, 532, 820, 835
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 266, 443
Eco147I AGGCCT 1 cut(s) 403
Eco24I GRGCYC 2 cut(s) 274, 919
Eco47I GGWCC 2 cut(s) 241, 411
Eco57I CTGAAG 3 cut(s) 42, 684, 722
EcoO109I RGGNCCY 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 100
EcoRII CCWGG 3 cut(s) 137, 171, 584
EcoT14I CCWWGG 2 cut(s) 266, 443
EcoT22I ATGCAT 1 cut(s) 39
EcoT38I GRGCYC 2 cut(s) 274, 919
ErhI CCWWGG 2 cut(s) 266, 443
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 8 cut(s) 37, 270, 290, 362, 430, 530, 796, 876
FaqI GGGAC 1 cut(s) 397
FatI CATG 8 cut(s) 33, 266, 286, 358, 426, 526, 792, 872
Fnu4HI GCNGC 5 cut(s) 31, 71, 380, 394, 397
FokI GGATG 1 cut(s) 424
FriOI GRGCYC 2 cut(s) 274, 919
Fsp4HI GCNGC 5 cut(s) 31, 71, 380, 394, 397
FspBI CTAG 7 cut(s) 84, 122, 390, 444, 459, 638, 668
GluI GCNGC 5 cut(s) 31, 71, 380, 394, 397
HaeIII GGCC 4 cut(s) 8, 403, 584, 755
HapII CCGG 1 cut(s) 9
Hin1II CATG 8 cut(s) 37, 270, 290, 362, 430, 530, 796, 876
HinfI GANTC 4 cut(s) 196, 253, 732, 812
HpaII CCGG 1 cut(s) 9
HphI GGTGA 2 cut(s) 653, 653
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 6 cut(s) 61, 99, 544, 570, 731, 811
Hpy188III TCNNGA 2 cut(s) 250, 625
Hpy8I GTNNAC 1 cut(s) 89
HpyAV CCTTC 5 cut(s) 66, 574, 622, 668, 746
HpyCH4III ACNGT 2 cut(s) 261, 807
HpyCH4V TGCA 4 cut(s) 37, 70, 530, 876
HpyF3I CTNAG 4 cut(s) 194, 543, 758, 879
Hsp92II CATG 8 cut(s) 37, 270, 290, 362, 430, 530, 796, 876
KpnI GGTACC 1 cut(s) 192
Kzo9I GATC 4 cut(s) 77, 532, 820, 835
LmnI GCTCC 5 cut(s) 27, 277, 767, 902, 922
Lsp1109I GCAGC 4 cut(s) 42, 82, 405, 408
LweI GCATC 2 cut(s) 46, 892
MaeI CTAG 7 cut(s) 84, 122, 390, 444, 459, 638, 668
MaeIII GTNAC 3 cut(s) 147, 454, 893
MalI GATC 4 cut(s) 79, 534, 822, 837
MboI GATC 4 cut(s) 77, 532, 820, 835
MboII GAAGA 5 cut(s) 72, 188, 269, 380, 697
MflI RGATCY 2 cut(s) 77, 835
MhlI GDGCHC 2 cut(s) 274, 919
MluCI AATT 4 cut(s) 100, 202, 371, 747
MlyI GAGTC 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 548
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 2 cut(s) 336, 940
MslI CAYNNNNRTG 1 cut(s) 544
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 3 cut(s) 139, 173, 586
MvaI CCWGG 3 cut(s) 139, 173, 586
NcoI CCATGG 1 cut(s) 266
NdeII GATC 4 cut(s) 77, 532, 820, 835
NlaIII CATG 8 cut(s) 37, 270, 290, 362, 430, 530, 796, 876
NlaIV GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 864, 918
NmuCI GTSAC 2 cut(s) 454, 893
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 2 cut(s) 37, 530
PaeI GCATGC 1 cut(s) 37
PceI AGGCCT 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 3 cut(s) 253, 732, 812
PflMI CCANNNNNTGG 1 cut(s) 24
PfoI TCCNGGA 1 cut(s) 137
PkrI GCNGC 5 cut(s) 32, 72, 381, 395, 398
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
PsiI TTATAA 1 cut(s) 305
Psp6I CCWGG 3 cut(s) 137, 171, 584
PspGI CCWGG 3 cut(s) 137, 171, 584
PspN4I GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 864, 918
PspPI GGNCC 3 cut(s) 241, 411, 754
PsuI RGATCY 2 cut(s) 77, 835
RsaI GTAC 2 cut(s) 190, 352
RsaNI GTAC 2 cut(s) 189, 351
RseI CAYNNNNRTG 1 cut(s) 544
SaqAI TTAA 2 cut(s) 336, 940
SatI GCNGC 5 cut(s) 31, 71, 380, 394, 397
Sau3AI GATC 4 cut(s) 77, 532, 820, 835
Sau96I GGNCC 3 cut(s) 241, 411, 754
ScaI AGTACT 1 cut(s) 352
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 3 cut(s) 139, 173, 586
SduI GDGCHC 2 cut(s) 274, 919
SfaNI GCATC 2 cut(s) 46, 892
SinI GGWCC 2 cut(s) 241, 411
SmiMI CAYNNNNRTG 1 cut(s) 544
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 2 cut(s) 458, 667
SphI GCATGC 1 cut(s) 37
Sse9I AATT 4 cut(s) 100, 202, 371, 747
SseBI AGGCCT 1 cut(s) 403
SsiI CCGC 1 cut(s) 380
SspI AATATT 2 cut(s) 230, 301
SspMI CTAG 7 cut(s) 84, 122, 390, 444, 459, 638, 668
StuI AGGCCT 1 cut(s) 403
StyD4I CCNGG 3 cut(s) 137, 171, 584
StyI CCWWGG 2 cut(s) 266, 443
TaaI ACNGT 2 cut(s) 261, 807
TasI AATT 4 cut(s) 100, 202, 371, 747
TatI WGTACW 1 cut(s) 350
TauI GCSGC 1 cut(s) 382
TfiI GAWTC 3 cut(s) 253, 732, 812
Tru1I TTAA 2 cut(s) 336, 940
Tru9I TTAA 2 cut(s) 336, 940
TscAI CASTG 4 cut(s) 562, 568, 700, 784
TseFI GTSAC 2 cut(s) 454, 893
TseI GCWGC 4 cut(s) 30, 70, 393, 396
Tsp45I GTSAC 2 cut(s) 454, 893
TspDTI ATGAA 1 cut(s) 426
TspRI CASTG 4 cut(s) 562, 568, 700, 784
Van91I CCANNNNNTGG 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 241, 411
XapI RAATTY 3 cut(s) 100, 371, 747
XceI RCATGY 2 cut(s) 37, 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
XmaJI CCTAGG 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 7 cut(s) 84, 122, 390, 444, 459, 638, 668
ZrmI AGTACT 1 cut(s) 352
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.