Rw2G032910
BHLH Family

Transcription factor bHLH68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001178
Physical Location & Seq
Reverse (-)
53984009 .. 53986715
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032910.1

Sequence Viewer

Length: 981 bp
ATGATGGCCGGAAACCCTAACTGGTGGAGCACGCATCCACCGTCATCTATCTTCCCTTCTCAATATGTGCTTGGATCTTCTAATTCACTCCCTCAGCTCAATTCATTCCCTGATCAGCATAACCTACAGCCTCCACAATCTTGGAGCCAATTACTCCTGGGCGGATTGTCCGGAGAAGATCAAGACAACTTTGGAAGTTTGAATCATTTTCCACCTAAAAGGTTGGAAAATTGGGAGGATCATCAGCAGCAAATATTGATTCCACAGTCGTATCCAAGGGTTAATCCTGTTGTTGACAGTAATATTATAAAGCAAGAGGTTAATTCCCAGAACAACAGCAACTTGTGTGGTGGAGATGGAGGAGAAGAATTTCAGGCACCTTATAATAATAGGCCATCTTGGTCAGCAGCACAAATCAACATGCCAGTTTCATCCCCTAGGTCTTGTATCACTAGTTTAAGTAGTAATAATATCTTGGACTTTTCCTATAACCAGAAAAACCAACATGCAGATCACTCATCTGAGTGCAATAGTCCTCCCACCGGCGGAATGTGCAAGAAAGCTAGGGTTCAACCATCTCCAAGCCAACCACCATTAAAGGTGAGAAAGGAGAAGCTAGGAGATAGAATAACAGCCCTTCATCAAATAGTTTCCCCATTTGGAAAGACTGACACTGCTTCTGTCTTGTTAGAAGCCATTGGGTATATCAGATTCCTCCAGAGTCAAATTGAGGCCCTCAGCTCCCCTTACTTGGGCAATGCCTCCAAAAACATGAGGAATAATCCCCACTCTCTGTTGAATGACAACTGCAGCCTGAAAAGAAAAGGGGCTTCTAACCAGGATTCTCATCAAGACTATAAGGCAAAGGATTTAAGAAGTAAAGGATTGTGTTTGGTTCCTGTTTCTTGCACTCAGAATGTTGGGGGCGACATTAACATTGGAGCTGATTATTGGGCTCCGGCTTATGGGAGCGGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

326

Amino Acids

35.83

Weight (kDa)

7.1

Isoelectric Point (pI)

64.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 308, 384
AccB1I GGYRCC 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 972
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 3 cut(s) 162, 546, 972
AclWI GGATC 2 cut(s) 82, 246
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 368
AfiI CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 965
AgsI TTSAA 3 cut(s) 202, 572, 799
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 2 cut(s) 156, 837
AluBI AGCT 5 cut(s) 97, 563, 616, 741, 944
AluI AGCT 5 cut(s) 97, 563, 616, 741, 944
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 2 cut(s) 82, 246
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 3 cut(s) 6, 392, 732
ApeKI GCWGC 3 cut(s) 247, 407, 810
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 369
AspA2I CCTAGG 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 733
AsuHPI GGTGA 1 cut(s) 613
AvrII CCTAGG 1 cut(s) 437
BaeI ACNNNNGTAYC 2 cut(s) 254, 287
BanI GGYRCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 958
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 2 cut(s) 93, 737
BbvI GCAGC 3 cut(s) 259, 419, 822
BccI CCATC 3 cut(s) 350, 403, 583
BciT130I CCWGG 2 cut(s) 158, 839
BciVI GTATCC 1 cut(s) 282
BclI TGATCA 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 4 cut(s) 438, 453, 564, 617
BfmI CTRYAG 2 cut(s) 125, 808
BfuI GTATCC 1 cut(s) 282
BisI GCNGC 3 cut(s) 248, 408, 811
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 3 cut(s) 249, 409, 812
Bme1390I CCNGG 2 cut(s) 158, 839
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 4 cut(s) 146, 378, 897, 957
BmrFI CCNGG 2 cut(s) 158, 839
BmsI GCATC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 701
Bpu10I CCTNAGC 2 cut(s) 93, 737
BsaBI GATNNNNATC 1 cut(s) 846
BsaJI CCNNGG 3 cut(s) 157, 275, 437
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 965
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 2 cut(s) 26, 425
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 846
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 2 cut(s) 158, 839
BseDI CCNNGG 3 cut(s) 157, 275, 437
BseGI GGATG 2 cut(s) 34, 431
BseJI GATNNNNATC 1 cut(s) 846
BseLI CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 965
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 4 cut(s) 107, 513, 751, 926
BseNI ACTGG 2 cut(s) 26, 425
BseRI GAGGAG 1 cut(s) 375
BseXI GCAGC 3 cut(s) 259, 419, 822
BshFI GGCC 3 cut(s) 8, 394, 734
BshNI GGYRCC 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 4 cut(s) 9, 171, 543, 959
BslI CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 965
BsnI GGCC 3 cut(s) 8, 394, 734
Bsp1286I GDGCHC 2 cut(s) 32, 958
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 5 cut(s) 74, 112, 178, 238, 511
BspACI CCGC 3 cut(s) 162, 546, 972
BspANI GGCC 3 cut(s) 8, 394, 734
BspCNI CTCAG 4 cut(s) 106, 514, 750, 925
BspEI TCCGGA 1 cut(s) 170
BspLI GGNNCC 4 cut(s) 146, 378, 897, 957
BspMAI CTGCAG 1 cut(s) 812
BspPI GGATC 2 cut(s) 82, 246
BspT107I GGYRCC 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 972
BsrDI GCAATG 1 cut(s) 763
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 2 cut(s) 26, 425
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 3 cut(s) 157, 275, 437
BssMI GATC 5 cut(s) 74, 112, 178, 238, 511
BssT1I CCWWGG 2 cut(s) 275, 437
Bst2UI CCWGG 2 cut(s) 158, 839
Bst4CI ACNGT 3 cut(s) 42, 267, 299
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 4 cut(s) 93, 522, 737, 912
BstF5I GGATG 2 cut(s) 34, 431
BstKTI GATC 5 cut(s) 77, 115, 181, 241, 514
BstMBI GATC 5 cut(s) 74, 112, 178, 238, 511
BstMWI GCNNNNNNNGC 1 cut(s) 552
BstNI CCWGG 2 cut(s) 158, 839
BstNSI RCATGY 2 cut(s) 424, 509
BstSCI CCNGG 2 cut(s) 156, 837
BstSFI CTRYAG 2 cut(s) 125, 808
BstV1I GCAGC 3 cut(s) 259, 419, 822
BstX2I RGATCY 1 cut(s) 74
BstXI CCANNNNNNTGG 1 cut(s) 141
BstYI RGATCY 1 cut(s) 74
BsuI GTATCC 1 cut(s) 282
BsuRI GGCC 3 cut(s) 8, 394, 734
BtsCI GGATG 2 cut(s) 34, 431
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 1 cut(s) 672
Cac8I GCNNGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 733
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 3 cut(s) 421, 506, 772
DdeI CTNAG 4 cut(s) 93, 522, 737, 912
DpnI GATC 5 cut(s) 76, 114, 180, 240, 513
DpnII GATC 5 cut(s) 74, 112, 178, 238, 511
EaeI YGGCCR 1 cut(s) 6
EciI GGCGGA 2 cut(s) 177, 561
Eco130I CCWWGG 2 cut(s) 275, 437
Eco24I GRGCYC 1 cut(s) 958
EcoO109I RGGNCCY 1 cut(s) 733
EcoRII CCWGG 2 cut(s) 156, 837
EcoT14I CCWWGG 2 cut(s) 275, 437
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 2 cut(s) 275, 437
FaeI CATG 3 cut(s) 424, 509, 775
FatI CATG 3 cut(s) 420, 505, 771
FauI CCCGC 1 cut(s) 965
FbaI TGATCA 1 cut(s) 112
Fnu4HI GCNGC 3 cut(s) 248, 408, 811
FokI GGATG 2 cut(s) 21, 418
FriOI GRGCYC 1 cut(s) 958
Fsp4HI GCNGC 3 cut(s) 248, 408, 811
FspBI CTAG 4 cut(s) 438, 453, 564, 617
GluI GCNGC 3 cut(s) 248, 408, 811
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 3 cut(s) 8, 394, 734
HapII CCGG 4 cut(s) 9, 171, 543, 959
Hin1II CATG 3 cut(s) 424, 509, 775
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 5 cut(s) 202, 259, 711, 721, 842
HpaII CCGG 4 cut(s) 9, 171, 543, 959
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 3 cut(s) 523, 710, 915
Hpy188III TCNNGA 4 cut(s) 171, 182, 718, 851
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 2 cut(s) 66, 647
HpyCH4III ACNGT 3 cut(s) 42, 267, 299
HpyCH4V TGCA 5 cut(s) 509, 528, 555, 810, 909
HpyF10VI GCNNNNNNNGC 1 cut(s) 552
HpyF3I CTNAG 4 cut(s) 93, 522, 737, 912
Hsp92II CATG 3 cut(s) 424, 509, 775
Kpn2I TCCGGA 1 cut(s) 170
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 5 cut(s) 74, 112, 178, 238, 511
LmnI GCTCC 6 cut(s) 27, 144, 746, 941, 961, 969
Lsp1109I GCAGC 3 cut(s) 259, 419, 822
LweI GCATC 1 cut(s) 43
MaeI CTAG 4 cut(s) 438, 453, 564, 617
MalI GATC 5 cut(s) 76, 114, 180, 240, 513
MbiI CCGCTC 1 cut(s) 972
MboI GATC 5 cut(s) 74, 112, 178, 238, 511
MboII GAAGA 4 cut(s) 43, 69, 188, 377
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 2 cut(s) 32, 958
MluCI AATT 7 cut(s) 82, 100, 149, 229, 322, 368, 726
MlyI GAGTC 1 cut(s) 730
MmeI TCCRAC 1 cut(s) 204
MroI TCCGGA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 369
MseI TTAA 6 cut(s) 282, 321, 458, 596, 872, 933
MslI CAYNNNNRTG 1 cut(s) 523
MspI CCGG 4 cut(s) 9, 171, 543, 959
MspR9I CCNGG 2 cut(s) 158, 839
MvaI CCWGG 2 cut(s) 158, 839
MwoI GCNNNNNNNGC 1 cut(s) 552
NdeII GATC 5 cut(s) 74, 112, 178, 238, 511
NlaIII CATG 3 cut(s) 424, 509, 775
NlaIV GGNNCC 4 cut(s) 146, 378, 897, 957
NspI RCATGY 2 cut(s) 424, 509
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 4 cut(s) 202, 259, 711, 842
PkrI GCNGC 3 cut(s) 249, 409, 812
PleI GAGTC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 729
PsiI TTATAA 2 cut(s) 308, 384
Psp6I CCWGG 2 cut(s) 156, 837
PspGI CCWGG 2 cut(s) 156, 837
PspN4I GGNNCC 4 cut(s) 146, 378, 897, 957
PspPI GGNCC 1 cut(s) 733
PstI CTGCAG 1 cut(s) 812
PsuI RGATCY 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 523
SaqAI TTAA 6 cut(s) 282, 321, 458, 596, 872, 933
SatI GCNGC 3 cut(s) 248, 408, 811
Sau3AI GATC 5 cut(s) 74, 112, 178, 238, 511
Sau96I GGNCC 1 cut(s) 733
SchI GAGTC 1 cut(s) 730
ScrFI CCNGG 2 cut(s) 158, 839
SduI GDGCHC 2 cut(s) 32, 958
SfaNI GCATC 1 cut(s) 43
SfcI CTRYAG 2 cut(s) 125, 808
SgrAI CRCCGGYG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 7 cut(s) 82, 100, 149, 229, 322, 368, 726
SsiI CCGC 3 cut(s) 162, 546, 972
SspI AATATT 2 cut(s) 255, 304
SspMI CTAG 4 cut(s) 438, 453, 564, 617
StyD4I CCNGG 2 cut(s) 156, 837
StyI CCWWGG 2 cut(s) 275, 437
TaaI ACNGT 3 cut(s) 42, 267, 299
TasI AATT 7 cut(s) 82, 100, 149, 229, 322, 368, 726
TfiI GAWTC 4 cut(s) 202, 259, 711, 842
Tru1I TTAA 6 cut(s) 282, 321, 458, 596, 872, 933
Tru9I TTAA 6 cut(s) 282, 321, 458, 596, 872, 933
TscAI CASTG 1 cut(s) 679
TseI GCWGC 3 cut(s) 247, 407, 810
TspDTI ATGAA 3 cut(s) 93, 420, 629
TspRI CASTG 1 cut(s) 679
XapI RAATTY 1 cut(s) 368
XceI RCATGY 2 cut(s) 424, 509
XmaJI CCTAGG 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 4 cut(s) 438, 453, 564, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.