pycom09g12450
BHLH Family

Transcription factor bHLH68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001178
Physical Location & Seq
Forward (+)
10978081 .. 10980670
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12450.7

Sequence Viewer

Length: 987 bp
ATGATGGCCGGAAACCCTAAGTGGTGGAGCAGCATGCATCCACCATCTCTTTACCCTTCTGAATATGTGCAGCTTGGATCTTCTAGTTCATTCCCTCTGAATTCTTTGCCTGACCATAACCTAGAGCCTCCACAATCCTGGAGCCAGTTACTCTTGGGTGGATTGTCCAGCCAGGAAGATAGCTTTGGTACCCTGAGTCATCATTTTCAACCTAAAAGTAGGTTGAAAAATTGGGAGGAGGACCAAATCTTGATTCCACAATATCCAAGGGCTCCTCTTCCTCCTCATGGTGTTGATAATATTATAAAGCAAGAGGCTAGTTCCCAGAACAGCATTAACTTATTGGGCAGTACTCGTCATGTAGACGAAGAATTTCAGCCGCAGGCACCTGGCAGCAGGCCGCCTGTTTGGTCCCCAGTTCAAATCATGCCAGTTTCATCCCCTAGGTCTTGTGTCACTAGTTCAAGTAATACTAATAATATATTGGAATTCTCCTATGACAAAACTGACAATAAGAGGAACCAACATGCAGATCACTCATCTGAGTGTAATAGCACTGCCACTGGTGGGATAACCAAGAAGGCCAGGGTTCAACAATCGCCAAGCCAACCACCTCTCAAGGTCAGGAAGGAGAAGCTAGGTGATAGGATAACAACCCTTCACCAACTAGTTTCCCCATTTGGGAAGACTGACACTGCTTCAGTCCTGTTAGAAGTCATTGGGTATATCAGATTCCTTCAGGGTCAAATTCAGACACTCAGCTCCCCTTACTTGGGCAGTGCCTCAAAAGACATGAGGAACCAACAGTCTCTGTTGGGTGACAACTGCTTGAAAAACAAAGGCGCTCCTAACCAGCACAAGATCGTAAGGCAAAGGATCTGGGAAGTAAAGGGTTGTGCTTGGTTCCTGTGTCATGCACTCAGCATGTTGGGAGTGACAATGGTGCTGATTATTGGGCTCCTGCTTATGGAAATGGGTTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

36.46

Weight (kDa)

8.75

Isoelectric Point (pI)

64.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 188
AccB1I GGYRCC 2 cut(s) 188, 385
AccI GTMKAC 1 cut(s) 363
AciI CCGC 2 cut(s) 380, 401
AclWI GGATC 2 cut(s) 85, 884
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 4 cut(s) 100, 371, 488, 747
AcuI CTGAAG 2 cut(s) 684, 722
AfaI GTAC 2 cut(s) 190, 352
AfiI CCNNNNNNNGG 6 cut(s) 287, 567, 681, 772, 773, 967
AgsI TTSAA 6 cut(s) 209, 226, 422, 465, 593, 832
AhlI ACTAGT 2 cut(s) 458, 667
AjnI CCWGG 4 cut(s) 137, 171, 388, 584
AjuI GAANNNNNNNTTGG 4 cut(s) 168, 200, 237, 269
AluBI AGCT 4 cut(s) 73, 183, 637, 762
AluI AGCT 4 cut(s) 73, 183, 637, 762
Alw26I GTCTC 1 cut(s) 813
AlwI GGATC 2 cut(s) 85, 884
AlwNI CAGNNNCTG 1 cut(s) 811
AoxI GGCC 3 cut(s) 6, 398, 582
ApeKI GCWGC 3 cut(s) 30, 70, 393
ApoI RAATTY 4 cut(s) 100, 371, 488, 747
Asp700I GAANNNNTTC 1 cut(s) 372
Asp718I GGTACC 1 cut(s) 188
AspA2I CCTAGG 1 cut(s) 443
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 241, 411
AsuHPI GGTGA 3 cut(s) 653, 653, 830
AvaII GGWCC 2 cut(s) 241, 411
AvrII CCTAGG 1 cut(s) 443
BanI GGYRCC 2 cut(s) 188, 385
BanII GRGCYC 2 cut(s) 274, 960
BbsI GAAGAC 1 cut(s) 692
BbvI GCAGC 3 cut(s) 42, 82, 405
BccI CCATC 1 cut(s) 52
BciT130I CCWGG 4 cut(s) 139, 173, 390, 586
BcoDI GTCTC 1 cut(s) 813
BcuI ACTAGT 2 cut(s) 458, 667
BfaI CTAG 7 cut(s) 84, 122, 318, 444, 459, 638, 668
BfoI RGCGCY 1 cut(s) 846
BisI GCNGC 5 cut(s) 31, 71, 380, 394, 401
BlnI CCTAGG 1 cut(s) 443
BlsI GCNGC 5 cut(s) 32, 72, 381, 395, 402
BmcAI AGTACT 1 cut(s) 352
Bme1390I CCNGG 4 cut(s) 139, 173, 390, 586
Bme18I GGWCC 2 cut(s) 241, 411
BmgT120I GGNCC 2 cut(s) 241, 411
BmiI GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 905, 959
BmrFI CCNGG 4 cut(s) 139, 173, 390, 586
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 692
BpmI CTGGAG 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 266, 443, 585
Bsc4I CCNNNNNNNGG 6 cut(s) 287, 567, 681, 772, 773, 967
Bse1I ACTGG 4 cut(s) 145, 416, 431, 568
BseBI CCWGG 4 cut(s) 139, 173, 390, 586
BseDI CCNNGG 3 cut(s) 266, 443, 585
BseGI GGATG 2 cut(s) 37, 437
BseLI CCNNNNNNNGG 6 cut(s) 287, 567, 681, 772, 773, 967
BseMII CTCAG 4 cut(s) 185, 534, 772, 934
BseNI ACTGG 4 cut(s) 145, 416, 431, 568
BseRI GAGGAG 3 cut(s) 251, 264, 273
BseXI GCAGC 3 cut(s) 42, 82, 405
BsgI GTGCAG 1 cut(s) 89
BshFI GGCC 3 cut(s) 8, 400, 584
BshNI GGYRCC 2 cut(s) 188, 385
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 6 cut(s) 287, 567, 681, 772, 773, 967
BsmAI GTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 3 cut(s) 8, 400, 584
Bsp1286I GDGCHC 2 cut(s) 274, 960
Bsp143I GATC 4 cut(s) 77, 532, 861, 876
BspACI CCGC 2 cut(s) 380, 401
BspANI GGCC 3 cut(s) 8, 400, 584
BspCNI CTCAG 4 cut(s) 186, 535, 771, 933
BspLI GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 905, 959
BspPI GGATC 2 cut(s) 85, 884
BspT107I GGYRCC 2 cut(s) 188, 385
BsrI ACTGG 4 cut(s) 145, 416, 431, 568
BssECI CCNNGG 3 cut(s) 266, 443, 585
BssMI GATC 4 cut(s) 77, 532, 861, 876
BssT1I CCWWGG 2 cut(s) 266, 443
Bst2UI CCWGG 4 cut(s) 139, 173, 390, 586
Bst4CI ACNGT 1 cut(s) 807
Bst6I CTCTTC 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 35, 384, 398
BstDEI CTNAG 5 cut(s) 18, 194, 543, 758, 920
BstF5I GGATG 2 cut(s) 37, 437
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 4 cut(s) 80, 535, 864, 879
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 4 cut(s) 77, 532, 861, 876
BstNI CCWGG 4 cut(s) 139, 173, 390, 586
BstNSI RCATGY 3 cut(s) 37, 530, 928
BstSCI CCNGG 4 cut(s) 137, 171, 388, 584
BstV1I GCAGC 3 cut(s) 42, 82, 405
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 2 cut(s) 77, 876
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 2 cut(s) 77, 876
BsuRI GGCC 3 cut(s) 8, 400, 584
BtsCI GGATG 2 cut(s) 37, 437
BtsI GCAGTG 3 cut(s) 555, 693, 784
BtsIMutI CAGTG 4 cut(s) 555, 561, 693, 784
Cac8I GCNNGC 3 cut(s) 35, 384, 398
CaiI CAGNNNCTG 1 cut(s) 811
CfoI GCGC 1 cut(s) 845
Cfr13I GGNCC 2 cut(s) 241, 411
Csp6I GTAC 2 cut(s) 189, 351
CviAII CATG 8 cut(s) 34, 287, 359, 427, 527, 793, 914, 925
CviQI GTAC 2 cut(s) 189, 351
DdeI CTNAG 5 cut(s) 18, 194, 543, 758, 920
DpnI GATC 4 cut(s) 79, 534, 863, 878
DpnII GATC 4 cut(s) 77, 532, 861, 876
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 266, 443
Eco24I GRGCYC 2 cut(s) 274, 960
Eco47I GGWCC 2 cut(s) 241, 411
Eco57I CTGAAG 2 cut(s) 684, 722
EcoRI GAATTC 2 cut(s) 100, 488
EcoRII CCWGG 4 cut(s) 137, 171, 388, 584
EcoT14I CCWWGG 2 cut(s) 266, 443
EcoT22I ATGCAT 1 cut(s) 39
EcoT38I GRGCYC 2 cut(s) 274, 960
ErhI CCWWGG 2 cut(s) 266, 443
FaeI CATG 8 cut(s) 37, 290, 362, 430, 530, 796, 917, 928
FaqI GGGAC 1 cut(s) 397
FatI CATG 8 cut(s) 33, 286, 358, 426, 526, 792, 913, 924
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 5 cut(s) 31, 71, 380, 394, 401
FokI GGATG 2 cut(s) 24, 424
FriOI GRGCYC 2 cut(s) 274, 960
Fsp4HI GCNGC 5 cut(s) 31, 71, 380, 394, 401
FspBI CTAG 7 cut(s) 84, 122, 318, 444, 459, 638, 668
GlaI GCGC 1 cut(s) 844
GluI GCNGC 5 cut(s) 31, 71, 380, 394, 401
GsuI CTGGAG 1 cut(s) 160
HaeII RGCGCY 1 cut(s) 846
HaeIII GGCC 3 cut(s) 8, 400, 584
HapII CCGG 1 cut(s) 9
HhaI GCGC 1 cut(s) 845
Hin1II CATG 8 cut(s) 37, 290, 362, 430, 530, 796, 917, 928
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HinfI GANTC 3 cut(s) 196, 253, 732
HpaII CCGG 1 cut(s) 9
HphI GGTGA 3 cut(s) 653, 653, 830
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 5 cut(s) 61, 99, 544, 731, 753
Hpy188III TCNNGA 2 cut(s) 250, 625
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 5 cut(s) 66, 574, 622, 668, 746
HpyCH4III ACNGT 1 cut(s) 807
HpyCH4V TGCA 4 cut(s) 37, 70, 530, 917
HpyF3I CTNAG 5 cut(s) 18, 194, 543, 758, 920
Hsp92II CATG 8 cut(s) 37, 290, 362, 430, 530, 796, 917, 928
HspAI GCGC 1 cut(s) 843
KpnI GGTACC 1 cut(s) 192
Kzo9I GATC 4 cut(s) 77, 532, 861, 876
LmnI GCTCC 6 cut(s) 27, 141, 277, 767, 850, 963
Lsp1109I GCAGC 3 cut(s) 42, 82, 405
LweI GCATC 1 cut(s) 46
MaeI CTAG 7 cut(s) 84, 122, 318, 444, 459, 638, 668
MaeIII GTNAC 4 cut(s) 147, 454, 818, 934
MalI GATC 4 cut(s) 79, 534, 863, 878
MboI GATC 4 cut(s) 77, 532, 861, 876
MboII GAAGA 5 cut(s) 72, 188, 269, 380, 697
MflI RGATCY 2 cut(s) 77, 876
MhlI GDGCHC 2 cut(s) 274, 960
MluCI AATT 6 cut(s) 100, 229, 371, 488, 747, 982
MlyI GAGTC 1 cut(s) 205
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 3 cut(s) 336, 981, 985
MslI CAYNNNNRTG 1 cut(s) 544
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 4 cut(s) 139, 173, 390, 586
MvaI CCWGG 4 cut(s) 139, 173, 390, 586
NdeII GATC 4 cut(s) 77, 532, 861, 876
NlaIII CATG 8 cut(s) 37, 290, 362, 430, 530, 796, 917, 928
NlaIV GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 905, 959
NmuCI GTSAC 3 cut(s) 454, 818, 934
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 3 cut(s) 37, 530, 928
PacI TTAATTAA 1 cut(s) 985
PaeI GCATGC 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 253, 732
PfoI TCCNGGA 1 cut(s) 137
PkrI GCNGC 5 cut(s) 32, 72, 381, 395, 402
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
PsiI TTATAA 1 cut(s) 305
Psp6I CCWGG 4 cut(s) 137, 171, 388, 584
PspGI CCWGG 4 cut(s) 137, 171, 388, 584
PspN4I GGNNCC 9 cut(s) 143, 190, 273, 387, 413, 521, 800, 905, 959
PspPI GGNCC 2 cut(s) 241, 411
PstNI CAGNNNCTG 1 cut(s) 811
PsuI RGATCY 2 cut(s) 77, 876
RsaI GTAC 2 cut(s) 190, 352
RsaNI GTAC 2 cut(s) 189, 351
RseI CAYNNNNRTG 1 cut(s) 544
SaqAI TTAA 3 cut(s) 336, 981, 985
SatI GCNGC 5 cut(s) 31, 71, 380, 394, 401
Sau3AI GATC 4 cut(s) 77, 532, 861, 876
Sau96I GGNCC 2 cut(s) 241, 411
ScaI AGTACT 1 cut(s) 352
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 4 cut(s) 139, 173, 390, 586
SduI GDGCHC 2 cut(s) 274, 960
SfaNI GCATC 1 cut(s) 46
SinI GGWCC 2 cut(s) 241, 411
SmiMI CAYNNNNRTG 1 cut(s) 544
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 2 cut(s) 458, 667
SphI GCATGC 1 cut(s) 37
Sse9I AATT 6 cut(s) 100, 229, 371, 488, 747, 982
SsiI CCGC 2 cut(s) 380, 401
SspI AATATT 1 cut(s) 301
SspMI CTAG 7 cut(s) 84, 122, 318, 444, 459, 638, 668
StyD4I CCNGG 4 cut(s) 137, 171, 388, 584
StyI CCWWGG 2 cut(s) 266, 443
TaaI ACNGT 1 cut(s) 807
TasI AATT 6 cut(s) 100, 229, 371, 488, 747, 982
TatI WGTACW 1 cut(s) 350
TauI GCSGC 2 cut(s) 382, 403
TfiI GAWTC 2 cut(s) 253, 732
Tru1I TTAA 3 cut(s) 336, 981, 985
Tru9I TTAA 3 cut(s) 336, 981, 985
TscAI CASTG 4 cut(s) 562, 568, 700, 784
TseFI GTSAC 3 cut(s) 454, 818, 934
TseI GCWGC 3 cut(s) 30, 70, 393
Tsp45I GTSAC 3 cut(s) 454, 818, 934
TspDTI ATGAA 2 cut(s) 78, 426
TspRI CASTG 4 cut(s) 562, 568, 700, 784
VpaK11BI GGWCC 2 cut(s) 241, 411
XapI RAATTY 4 cut(s) 100, 371, 488, 747
XceI RCATGY 3 cut(s) 37, 530, 928
XmaJI CCTAGG 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 363
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 7 cut(s) 84, 122, 318, 444, 459, 638, 668
ZrmI AGTACT 1 cut(s) 352
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.