MD15G1191400.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001178
Physical Location & Seq
Reverse (-)
15056169 .. 15060584
4416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1191400.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGAATAGAGGTGGTTGCCAGAGCTCACCGGTGCAACAAATGATTGCCGGAAACCCTAACTGGTGGAGTATCAACAGCATGAGGCCACCAAGTACCCAACCATCTATGAATATTTCTCCTTTTTTTCAGCCTACTCCTCCTCCTCTTCCTCCTAATTTTTTAATCCCACAATTTGCACCCAGCAACAACAACACTTCCCCTTCTTCAACAACTTCTTCTTCAGGACTTGCCATTCCTTCTTGGCATATTGAGAATAACAACCAGGAGCTTCCAGAGTCTTGGAGCCACCTCCTCTTGGGTGGAATTGTAGGTGAAGATGAGAAAGGTGCAATCATGAGACAATTTCATCAAGCTGCTAAGAAATTGGAAAATTGGGATGAGCAAATATTAAATAGCACTCAAGCTCCAAATGCTCCTGTTGTGGACGTGAAGCAAGAAAACTCAGGAAATAGCAACGGCTTTGTGTACGGACATCATCACGGGAATGATCACATCCAAGGTTTTAATCCATCAGCTGCAGCTTTGTCTTCTTTGTCTCAGATTATGCAATCAACTGCTGCCTCTGCAGCCTCATCTCCTAAGTCATCGTGTGTGACGAGTTTCAGCAACAACAATATTTTGGATTTTTCTAGCATGTCGGACGTGAGGCATCCACCACCACATCGGTCCTCTGATCAGTGTAACAGCACTGGAAATGGTGGGGCACTTAAGAGAGCAAGGGTTCAACCTTCTTCAACCCCAACTACCTTCAAGGTGAGGAAGGAGAAATTAGGTGACAGAATTACAGCCCTTCATCAGCTAGTTTCCCCATTTGGGAAGACTGACACAGCCTCTGTTTTGTTAGAAGCTATTGGGTACATCAGATTCCTTCAGAGTCAAATTGAGGCCCTCAGTTCACCTTACTTGGGCAGTGGATCAGGAAGCATGAGGCAGCAGCCACAGTCTGCTGTTCATGGGGAGAGGAATTGTATGTTCCCTGAAGATCCTGGTCAGTTGCTCAATGACAATTGCATGAAACGGAAAGGAGCTTCTGAGCAGGATCCTCATGAAGAGCCAAAAAAGGACCTGAGGAGTAGGGGCTTGTGTTTGGTTCCAGTTTCATGCACCCTCCAAGTTGGGAGCGATAACGGAGCTGACTATTGGGCTCCGGCGCTCGGCTGTGGAGGTTTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

392

Amino Acids

42.14

Weight (kDa)

6.63

Isoelectric Point (pI)

62.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 922, 977, 1034, 1047
AcuI CTGAAG 3 cut(s) 204, 854, 999
AfaI GTAC 3 cut(s) 94, 467, 857
AfiI CCNNNNNNNGG 4 cut(s) 295, 813, 905, 1154
AflII CTTAAG 1 cut(s) 707
AgeI ACCGGT 1 cut(s) 28
AgsI TTSAA 4 cut(s) 207, 725, 735, 751
AjiI CACGTC 2 cut(s) 427, 643
AjnI CCWGG 2 cut(s) 261, 985
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 2 cut(s) 331, 540
AlwI GGATC 4 cut(s) 922, 977, 1034, 1047
AlwNI CAGNNNCTG 1 cut(s) 833
AoxI GGCC 2 cut(s) 83, 885
ApeKI GCWGC 7 cut(s) 353, 515, 518, 557, 566, 931, 934
AsiGI ACCGGT 1 cut(s) 28
AspLEI GCGC 1 cut(s) 1153
AspS9I GGNCC 3 cut(s) 666, 886, 1063
AsuHPI GGTGA 5 cut(s) 18, 323, 766, 785, 888
AvaII GGWCC 2 cut(s) 666, 1063
AxyI CCTNAGG 1 cut(s) 1067
BaeGI GKGCMC 1 cut(s) 706
BamHI GGATCC 1 cut(s) 1039
BanII GRGCYC 2 cut(s) 26, 1147
BbsI GAAGAC 2 cut(s) 519, 824
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 7 cut(s) 340, 502, 530, 544, 578, 943, 946
BccI CCATC 2 cut(s) 109, 517
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 2 cut(s) 263, 987
BclI TGATCA 2 cut(s) 487, 673
BcoDI GTCTC 2 cut(s) 331, 540
BfaI CTAG 2 cut(s) 630, 800
BfmI CTRYAG 2 cut(s) 516, 564
BfoI RGCGCY 1 cut(s) 1154
BfrI CTTAAG 1 cut(s) 707
BisI GCNGC 7 cut(s) 354, 516, 519, 558, 567, 932, 935
BlsI GCNGC 7 cut(s) 355, 517, 520, 559, 568, 933, 936
Bme1390I CCNGG 2 cut(s) 263, 987
Bme18I GGWCC 2 cut(s) 666, 1063
BmgBI CACGTC 2 cut(s) 427, 643
BmgT120I GGNCC 3 cut(s) 666, 886, 1063
BmiI GGNNCC 4 cut(s) 284, 1041, 1092, 1146
BmrFI CCNGG 2 cut(s) 263, 987
BmsI GCATC 1 cut(s) 658
BpiI GAAGAC 2 cut(s) 519, 824
BpuEI CTTGAG 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 496
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 388, 418
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 813, 905, 1154
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 3 cut(s) 65, 694, 1094
Bse21I CCTNAGG 1 cut(s) 1067
BseBI CCWGG 2 cut(s) 263, 987
BseDI CCNNGG 1 cut(s) 496
BseGI GGATG 3 cut(s) 382, 492, 649
BseLI CCNNNNNNNGG 4 cut(s) 295, 813, 905, 1154
BseMII CTCAG 5 cut(s) 456, 551, 904, 1023, 1058
BseNI ACTGG 3 cut(s) 65, 694, 1094
BseRI GAGGAG 5 cut(s) 126, 129, 132, 281, 1084
BseSI GKGCMC 1 cut(s) 706
BseXI GCAGC 7 cut(s) 340, 502, 530, 544, 578, 943, 946
BseYI CCCAGC 1 cut(s) 179
BshFI GGCC 2 cut(s) 85, 887
BshTI ACCGGT 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 26
BsiSI CCGG 3 cut(s) 29, 48, 1148
BslI CCNNNNNNNGG 4 cut(s) 295, 813, 905, 1154
BsmAI GTCTC 2 cut(s) 331, 540
BsnI GGCC 2 cut(s) 85, 887
Bsp1286I GDGCHC 3 cut(s) 26, 706, 1147
Bsp143I GATC 5 cut(s) 487, 673, 914, 982, 1039
BspANI GGCC 2 cut(s) 85, 887
BspCNI CTCAG 5 cut(s) 455, 550, 903, 1024, 1059
BspHI TCATGA 2 cut(s) 333, 1045
BspLI GGNNCC 4 cut(s) 284, 1041, 1092, 1146
BspMAI CTGCAG 2 cut(s) 520, 568
BspPI GGATC 4 cut(s) 922, 977, 1034, 1047
BspQI GCTCTTC 1 cut(s) 1044
BspTI CTTAAG 1 cut(s) 707
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 3 cut(s) 65, 694, 1094
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 496
BssMI GATC 5 cut(s) 487, 673, 914, 982, 1039
BssT1I CCWWGG 1 cut(s) 496
Bst2UI CCWGG 2 cut(s) 263, 987
Bst4CI ACNGT 1 cut(s) 942
Bst6I CTCTTC 2 cut(s) 150, 1044
BstAFI CTTAAG 1 cut(s) 707
BstDEI CTNAG 7 cut(s) 357, 442, 537, 579, 890, 1032, 1067
BstF5I GGATG 3 cut(s) 382, 492, 649
BstH2I RGCGCY 1 cut(s) 1154
BstHHI GCGC 1 cut(s) 1153
BstKTI GATC 5 cut(s) 490, 676, 917, 985, 1042
BstMAI GTCTC 2 cut(s) 331, 540
BstMBI GATC 5 cut(s) 487, 673, 914, 982, 1039
BstMWI GCNNNNNNNGC 3 cut(s) 410, 563, 566
BstNI CCWGG 2 cut(s) 263, 987
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 2 cut(s) 261, 985
BstSFI CTRYAG 2 cut(s) 516, 564
BstSLI GKGCMC 1 cut(s) 706
BstV1I GCAGC 7 cut(s) 340, 502, 530, 544, 578, 943, 946
BstV2I GAAGAC 2 cut(s) 519, 824
BstX2I RGATCY 2 cut(s) 982, 1039
BstXI CCANNNNNNTGG 1 cut(s) 279
BstYI RGATCY 2 cut(s) 982, 1039
Bsu36I CCTNAGG 1 cut(s) 1067
BsuRI GGCC 2 cut(s) 85, 887
BtrI CACGTC 2 cut(s) 427, 643
BtsCI GGATG 3 cut(s) 382, 492, 649
BtsI GCAGTG 1 cut(s) 916
BtsIMutI CAGTG 3 cut(s) 683, 687, 916
CaiI CAGNNNCTG 1 cut(s) 833
CciI TCATGA 2 cut(s) 333, 1045
CfoI GCGC 1 cut(s) 1153
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 3 cut(s) 666, 886, 1063
Csp6I GTAC 3 cut(s) 93, 466, 856
CspAI ACCGGT 1 cut(s) 28
CviAII CATG 8 cut(s) 79, 334, 634, 925, 953, 1012, 1046, 1101
CviQI GTAC 3 cut(s) 93, 466, 856
DdeI CTNAG 7 cut(s) 357, 442, 537, 579, 890, 1032, 1067
DpnI GATC 5 cut(s) 489, 675, 916, 984, 1041
DpnII GATC 5 cut(s) 487, 673, 914, 982, 1039
Eam1104I CTCTTC 2 cut(s) 150, 1044
EarI CTCTTC 2 cut(s) 150, 1044
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 496
Eco24I GRGCYC 2 cut(s) 26, 1147
Eco47I GGWCC 2 cut(s) 666, 1063
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 3 cut(s) 204, 854, 999
Eco81I CCTNAGG 1 cut(s) 1067
EcoICRI GAGCTC 1 cut(s) 24
EcoO109I RGGNCCY 2 cut(s) 886, 1063
EcoRII CCWGG 2 cut(s) 261, 985
EcoT14I CCWWGG 1 cut(s) 496
EcoT38I GRGCYC 2 cut(s) 26, 1147
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 8 cut(s) 82, 337, 637, 928, 956, 1015, 1049, 1104
FatI CATG 8 cut(s) 78, 333, 633, 924, 952, 1011, 1045, 1100
FbaI TGATCA 2 cut(s) 487, 673
Fnu4HI GCNGC 7 cut(s) 354, 516, 519, 558, 567, 932, 935
FokI GGATG 3 cut(s) 389, 479, 636
FriOI GRGCYC 2 cut(s) 26, 1147
Fsp4HI GCNGC 7 cut(s) 354, 516, 519, 558, 567, 932, 935
FspBI CTAG 2 cut(s) 630, 800
GlaI GCGC 1 cut(s) 1152
GluI GCNGC 7 cut(s) 354, 516, 519, 558, 567, 932, 935
GsaI CCCAGC 1 cut(s) 183
HaeII RGCGCY 1 cut(s) 1154
HaeIII GGCC 2 cut(s) 85, 887
HapII CCGG 3 cut(s) 29, 48, 1148
HhaI GCGC 1 cut(s) 1153
Hin1II CATG 8 cut(s) 82, 337, 637, 928, 956, 1015, 1049, 1104
Hin6I GCGC 1 cut(s) 1151
HinP1I GCGC 1 cut(s) 1151
HinfI GANTC 3 cut(s) 275, 864, 874
HpaII CCGG 3 cut(s) 29, 48, 1148
HphI GGTGA 5 cut(s) 18, 323, 766, 785, 888
Hpy166II GTNNAC 3 cut(s) 424, 466, 896
Hpy188I TCNGA 6 cut(s) 540, 640, 673, 863, 873, 1033
Hpy188III TCNNGA 6 cut(s) 222, 272, 334, 444, 918, 1046
Hpy8I GTNNAC 3 cut(s) 424, 466, 896
HpyAV CCTTC 7 cut(s) 210, 246, 738, 754, 757, 800, 878
HpyCH4III ACNGT 1 cut(s) 942
HpyCH4IV ACGT 2 cut(s) 426, 642
HpyCH4V TGCA 8 cut(s) 34, 176, 329, 518, 547, 566, 1011, 1104
HpyF10VI GCNNNNNNNGC 3 cut(s) 410, 563, 566
HpyF3I CTNAG 7 cut(s) 357, 442, 537, 579, 890, 1032, 1067
HpySE526I ACGT 2 cut(s) 426, 642
Hsp92II CATG 8 cut(s) 82, 337, 637, 928, 956, 1015, 1049, 1104
HspAI GCGC 1 cut(s) 1151
Ksp22I TGATCA 2 cut(s) 487, 673
Kzo9I GATC 5 cut(s) 487, 673, 914, 982, 1039
LguI GCTCTTC 1 cut(s) 1044
LmnI GCTCC 8 cut(s) 265, 282, 409, 418, 1025, 1119, 1130, 1150
Lsp1109I GCAGC 7 cut(s) 340, 502, 530, 544, 578, 943, 946
LweI GCATC 1 cut(s) 658
MaeI CTAG 2 cut(s) 630, 800
MaeII ACGT 2 cut(s) 426, 642
MaeIII GTNAC 3 cut(s) 592, 680, 773
MalI GATC 5 cut(s) 489, 675, 916, 984, 1041
MboI GATC 5 cut(s) 487, 673, 914, 982, 1039
MfeI CAATTG 1 cut(s) 1006
MflI RGATCY 2 cut(s) 982, 1039
MhlI GDGCHC 3 cut(s) 26, 706, 1147
MlyI GAGTC 2 cut(s) 284, 883
MmeI TCCRAC 1 cut(s) 618
MseI TTAA 4 cut(s) 161, 389, 504, 708
MslI CAYNNNNRTG 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 515
MspCI CTTAAG 1 cut(s) 707
MspI CCGG 3 cut(s) 29, 48, 1148
MspR9I CCNGG 2 cut(s) 263, 987
MunI CAATTG 1 cut(s) 1006
MvaI CCWGG 2 cut(s) 263, 987
MwoI GCNNNNNNNGC 3 cut(s) 410, 563, 566
NdeII GATC 5 cut(s) 487, 673, 914, 982, 1039
NlaIII CATG 8 cut(s) 82, 337, 637, 928, 956, 1015, 1049, 1104
NlaIV GGNNCC 4 cut(s) 284, 1041, 1092, 1146
NmeAIII GCCGAG 1 cut(s) 1134
NmuCI GTSAC 2 cut(s) 592, 773
NspI RCATGY 1 cut(s) 637
PagI TCATGA 2 cut(s) 333, 1045
PciSI GCTCTTC 1 cut(s) 1044
PcsI WCGNNNNNNNCGW 1 cut(s) 593
PfeI GAWTC 1 cut(s) 864
PinAI ACCGGT 1 cut(s) 28
PkrI GCNGC 7 cut(s) 355, 517, 520, 559, 568, 933, 936
PleI GAGTC 2 cut(s) 283, 882
PpsI GAGTC 2 cut(s) 283, 882
PpuMI RGGWCCY 1 cut(s) 1063
Psp124BI GAGCTC 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 1063
Psp6I CCWGG 2 cut(s) 261, 985
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 2 cut(s) 261, 985
PspN4I GGNNCC 4 cut(s) 284, 1041, 1092, 1146
PspPI GGNCC 3 cut(s) 666, 886, 1063
PspPPI RGGWCCY 1 cut(s) 1063
PstI CTGCAG 2 cut(s) 520, 568
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 2 cut(s) 982, 1039
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 3 cut(s) 94, 467, 857
RsaNI GTAC 3 cut(s) 93, 466, 856
RseI CAYNNNNRTG 1 cut(s) 483
SacI GAGCTC 1 cut(s) 26
SapI GCTCTTC 1 cut(s) 1044
SaqAI TTAA 4 cut(s) 161, 389, 504, 708
SatI GCNGC 7 cut(s) 354, 516, 519, 558, 567, 932, 935
Sau3AI GATC 5 cut(s) 487, 673, 914, 982, 1039
Sau96I GGNCC 3 cut(s) 666, 886, 1063
SchI GAGTC 2 cut(s) 284, 883
ScrFI CCNGG 2 cut(s) 263, 987
SduI GDGCHC 3 cut(s) 26, 706, 1147
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 2 cut(s) 516, 564
SgrAI CRCCGGYG 1 cut(s) 28
SinI GGWCC 2 cut(s) 666, 1063
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 2 cut(s) 399, 707
SmoI CTYRAG 2 cut(s) 399, 707
SspI AATATT 3 cut(s) 112, 387, 616
SspMI CTAG 2 cut(s) 630, 800
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 261, 985
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 1 cut(s) 942
TaiI ACGT 2 cut(s) 429, 645
TaqII GACCGA 1 cut(s) 654
TfiI GAWTC 1 cut(s) 864
Tru1I TTAA 4 cut(s) 161, 389, 504, 708
Tru9I TTAA 4 cut(s) 161, 389, 504, 708
TscAI CASTG 3 cut(s) 683, 694, 916
TseFI GTSAC 2 cut(s) 592, 773
TseI GCWGC 7 cut(s) 353, 515, 518, 557, 566, 931, 934
Tsp45I GTSAC 2 cut(s) 592, 773
TspDTI ATGAA 8 cut(s) 17, 122, 335, 782, 941, 1028, 1062, 1089
TspGWI ACGGA 3 cut(s) 483, 1033, 1143
TspRI CASTG 3 cut(s) 683, 694, 916
Vha464I CTTAAG 1 cut(s) 707
VpaK11BI GGWCC 2 cut(s) 666, 1063
XceI RCATGY 1 cut(s) 637
XspI CTAG 2 cut(s) 630, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.