Rorug02G0014800

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001178
Physical Location & Seq
Reverse (-)
1246913 .. 1260018
13106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0014800.1

Sequence Viewer

Length: 4953 bp
ATGGACGACGCGCCGCCCGATGCGGGTCGTCCACCGCCGAAGAACGGTGGGGAAGAGGAGGGTCGGGCTGTGGAGTCGGGTGATGGAGCTCCGAGGCCGGAGAAGGATCCCAGGAAGGTTGCCCGGAGGTATCAATTGGAGCTGTGTCAGAAAGCTGTGGAGGAGAACATAATCGTCTATTTGGAAACTGGTTGTGGAAAGACCCACATAGCAGTGTTGCTCGTGTATGAGCTGCGCGAGTTGATAATGAAACCTCATAAGAGCATATGTGTGTTTCTTGCGCCTACGGTGGCGCTTGTTCAACAGCAAGCAAGAGTTATAGAAGATTCCCTTGATCTCAAAGTTGGGACCTATTGTGGAAGCTCTAAGCATTTGAAAACTCACCAAGACTGGGAAAAAGAGATGGAGCAATATGAGGTTTTTGTCATGACGCCTCAAATACTGTTACGTAACTTATACCACCGTATTATTAAGATGGAGTTGATTGCATTGCTGATTTTTGATGAGTGTCATCACGCTCAAATGAAAAGCAACCATCCTTATGCAGAAATTATGAAATTTTACAAGAGTGATGCCACTAAACTTCCTCGTATATTTGGCATGACTGCATCTCCAGTTGTAGGTAAAGGCACTTCTAGTCAATCGAATTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATACGGTTGAAGACAAGACAGAATTGCATTGTTCTGTTGTTTCTCCTGTTATCAATGTCCATCGTTATGGTCCTGTTATTAGAGGCACATCCAGCCCCTGCATGACTCTTTCTAGAGAACTTGACCAGCTGAAACGCCAGTGTATAGCAGAACTTAGTAAGAAGACAAGTGACTACCAAAGTCTAAGGAGTACAAAGAAAACGTTAAACAGAGTGCATGACAGTATAATATTTTGCTTGGAAAGTCTTGGCCTCTGGGGTGCGTTACAAGCTAGACATATTCTTTCGAGTGGCGATAACTTTGAACGAAATGCATTAATAGAGGAAGGCAATAATGATACAGAGGCAGAAGGAAATAATAGTGATGACTCTGTATGTGCGGAATATCTTGCTCAGGCAGCTGACATCATTCGTACTGACTGTGGGAAAGATGCTGTTGCATCTGGTCTATCTTGTGTAGAGATCTTAAAGGAGCCATTCTTCTCAAGTAAGGTTTTACGTCTTATTGGAATTCTTTCATCCTTCAGGTTGCAGCAGGATATGAAATGCATAATTTTTGTCAATAGGATTATTACTGCGAGATCCTTGTCCTACATACTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCGAAACTGAAGAGTATGTCAAGGAAGACGATGCAGATCATTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGACTAGATATTCAGACTTGCTGCCTTGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATATATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTGGATTTAATAGAAAACTTCAGGAAAGATGAAGAGCGAATGAACATGGAAGTTGCTTTCAGAACATCCAGTGAGACTTTTCTTAGTCCTGAGGAACGAACATATAAAGTTGCTTCTACTGGAGCTTCCGTCACTTCTGGATATAGCATCTCATTGTTACACCAGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCACTCCAGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCAGATAATTTTACCTTCCAATGCTCCAATGCATCAAATTGTCAGTACACCACAATCTTCGATGGAAGATGCCAAAAAAGATGCATGTCTAAAAGCCATCGAAGAATTGCATAAATTGGGTGCCTTAAGTGACTTTCTCTTGCCACAGCAAGACAATGCAAATGTGGAGGAGTCCATGCTAGACTCTTCTGATTATGACAGCAATGAAGATGAAGACTTACGAGAGGAACTACATGAAATGCTGGTTCCTGCTGTCCTAAAAGAGTCATGGAATAAGTTGGAGGATCTTGTCACCCTCAGCGCTTACTATTTTCAATTTGATCCTGAACCTAATGACAGAATCTATAGAAGTTTTGGTCTATTTGTCAAAGCACCTCTTCCAGCAGAGGCCGAAACAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCAGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGCTCATTCTGGACCGATCAGAATTTGTTTCTGAATTTGTTCCCCTGGGAAACTATGATTTTAGCAGATCGAGCACATCAACCTTCTACCTATTGCTTCCAGTAACTTTGGGTGAAAATGATAAGATAAGCATAGATTGGAGGATTATCAAAAAATGTTTATCATCTCCAATATTCAGGGGTCCAGGAGATGCTATGGACAGTAAAATTATATCTTCGGGTATTCGACTTGCATCTGGTTATAGAAGCATAAGTGAAGTTGAGGATAGTTTAGTTTATGTCCCATATAAGAAGACATTTTACTTCATCACTAATGTTGCTCGAGAAAGAAATGCCTACAGCCAATATAAAGAAAAGTCAGATGCTTTAACCTATGTGGATCATCTAAGTAAAAAGTTTGACATTTCTCTTAAATACCCTCAGCAACATCTTCTGTGTGCAAAACCAGTTTTCAATTTGCACAACTTGCTACACAATCGTAGGCAAGAAGATTCAGAAGCACAGCAACTAGATGAGTATTATATATATGTGCCTCCTGAGCTTTGCGAGCTGAAGGTAATTGGCTTTTCAAAAGATATTGGGAGTTCGGTATCTCTGTTACCATCAATCATGCACCATCTGGAAAACTTGCTTGTCGCCATCGAACTGAAGCATGTATTATGCACTTCATTCCCCGAAGGAGCTGAGGTTACTTCGCAGAGAGTTCTAGAAGCCCTCACAACTGAAAAGTGCCAGGAGCGGATTTCCCTTGAAAGGCTTGAAATACTAGGAGATGCTTTCCTCAAATTTGCAGTTGGAAGGCACTTTTTCCTTTTGCATGCTTCACTTGATGAAGGTGAACTAACAAGGAAACGTTCAAATGTGGTAAACAATTCCAATTTATTGAAGCTTGCTACTAGAAGCAACTTACAGGTATACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTCGCTTTAGGCCGCCCTTGCAAAAATATTTGTGACCAGGAGACAATAGGATCTCTTGATTCTCAAGATTTAAGCAGTGCAGTAAAACAGTCACATGCTCGTCACGTCAGATGTAGTAAGGGTCACCATTGGTTACATAAGAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCGGCAACTGCATTTCTCAGATGGATTGGCATAAAAGTAAACTTTGAAGCATCAGAAGTTACAAAAGTATGCATAGCAAGCAGCAGATACGTTTCACTTGCTGCTTGCATAGACATTGCGGCCCTTGAAAATTCATTAGGGTATCAGTTTCTCCACAGAGGTTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAACATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTGTATCCAAAGTTAAAGCCTGGCCATTTGACAGATTTGAGATCAATTGCTGTGAATAATAAAGCCTTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTACTCTCTGACACCAGTACCCTTTCTGAGGCTATAAAGACTTATGTGAACTTCATTGAGACATCTGCATCAGATAGTAGTCTGGTTGATGGGCCGAAATGCCCCAAGGCTCTGGGCGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGGCTTGACTTGAACCTTGTTTGGAAGATAATGCTATCCTTTCTGGAGCCTGTCATGAGCTTTTCCAACATTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGGTTCTTAAGTTTTTATCATCAAAGAAGGGTAAATCATTTTCAGTTGAGGCAACAGTGAATGGGAATAATGTTTCTGCAACTGCTTCCTCAACTGCCTTGAACAAAAAGGAGGCTATTAGAATTTCTGCTCAGTTAATTTTCGAAACGTTGAAGGCTCAAGGCAATATACCAAAGTCTAAGTTGTCTTTGGAGGAAGTCTTGAAGTCAAGCTCCAAGTTGGAACCCAAACTTATCGGATATGATGAAACACCAATAGATGTTACAGCTCTTGATATTATTGGATTTGATAAGTTAAATGTACAAGAGGCTTCTAGCAGCAGTTCCAACTCAGATGTGCAGTCTATGAGTGAAGTTAGCAGTGGTAACTCCTCCTGCATAAAACGTTTACAACAACCCCCAGCTTCACTTGGAGAAGTCAAAATGGACGGCACTAATGCCTGTAACAGTCGTAGCTCTGATGCTATCTCACAAACCGGAGGTGTGCCACACAAATTAACAGCAAAATCTCGTTTGCATGAAACCTGTGTTGCTAACTACTGGGAACCCCCTGAATTTGAATGTTGCAAAGAGGAAGGACCAGGCCACATAAAATCGTTCACTTTCAAGGTTACAGTGAAGATAGATGATGTTTCGGATACACTTTTGGAGGCCTTTGGAGAACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGGGCACTTTGGTACTTGGAAAAGAAAGGTTACATAAAGGAGAGTGATCAAACAGAGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1650

Amino Acids

184.49

Weight (kDa)

5.86

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 42 - 205 1.5e-16 Type III restriction enzyme, res subunit
DEAD PF00270 43 - 205 2e-18 DEAD/DEAH box helicase
Helicase_C PF00271 400 - 513 9e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 580 - 665 1.9e-23 Dicer dimerisation domain
PAZ PF02170 873 - 993 5.5e-09 PAZ domain
Ribonucleas_3_3 PF14622 1037 - 1095 5.6e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1038 - 1170 4.1e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1226 - 1357 2.1e-13 Ribonuclease-III-like
Ribonuclease_3 PF00636 1246 - 1355 3.6e-21 Ribonuclease III domain
DND1_DSRM PF14709 1561 - 1636 2.6e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1562 - 1632 4.4e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1961, 2290
AccBSI CCGCTC 1 cut(s) 3100
AccI GTMKAC 1 cut(s) 3276
AccII CGCG 2 cut(s) 11, 237
AclI AACGTT 4 cut(s) 899, 3214, 4331, 4567
AclWI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
AcoI YGGCCR 1 cut(s) 3820
AcuI CTGAAG 5 cut(s) 1204, 1379, 1597, 2933, 3029
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 8 cut(s) 889, 1111, 1888, 3699, 3922, 4485, 4852, 4901
AfeI AGCGCT 1 cut(s) 2143
AfiI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
AflII CTTAAG 3 cut(s) 1304, 1966, 4190
AjiI CACGTC 1 cut(s) 3418
AjnI CCWGG 7 cut(s) 110, 2406, 2545, 3093, 3348, 3817, 4762
AjuI GAANNNNNNNTTGG 2 cut(s) 3468, 3500
Alw21I GWGCWC 3 cut(s) 91, 1540, 2438
Alw26I GTCTC 3 cut(s) 1661, 3347, 3956
Alw44I GTGCAC 1 cut(s) 1536
AlwI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
AlwNI CAGNNNCTG 2 cut(s) 795, 1505
Ama87I CYCGRG 1 cut(s) 2682
Aor51HI AGCGCT 1 cut(s) 2143
AoxI GGCC 9 cut(s) 95, 946, 2229, 3322, 3636, 3820, 3995, 4765, 4833
ApaLI GTGCAC 1 cut(s) 1536
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 2 cut(s) 1210, 2400
AspLEI GCGC 5 cut(s) 13, 237, 283, 295, 2144
AspS9I GGNCC 7 cut(s) 348, 767, 2374, 2543, 3637, 3995, 4760
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 9 cut(s) 92, 374, 1457, 1832, 2125, 2486, 3209, 3428, 3772
AsuII TTCGAA 2 cut(s) 1766, 4326
AsuNHI GCTAGC 1 cut(s) 1511
AvaI CYCGRG 1 cut(s) 2682
AvaII GGWCC 5 cut(s) 348, 767, 2374, 2543, 4760
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 2 cut(s) 1540, 4894
BaeI ACNNNNGTAYC 2 cut(s) 3904, 3937
BalI TGGCCA 1 cut(s) 3822
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 2 cut(s) 1961, 2290
BanII GRGCYC 1 cut(s) 91
BarI GAAGNNNNNNTAC 4 cut(s) 616, 648, 2645, 2677
BauI CACGAG 2 cut(s) 221, 1527
BbsI GAAGAC 5 cut(s) 714, 866, 1382, 2061, 2660
Bbv12I GWGCWC 3 cut(s) 91, 1540, 2438
BbvCI CCTCAGC 3 cut(s) 2138, 2781, 3045
BceAI ACGGC 1 cut(s) 4627
BciT130I CCWGG 7 cut(s) 112, 2408, 2547, 3095, 3350, 3819, 4764
BciVI GTATCC 2 cut(s) 3813, 4813
BclI TGATCA 2 cut(s) 3777, 4933
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 3 cut(s) 1661, 3347, 3956
BfmI CTRYAG 2 cut(s) 2185, 2698
BfoI RGCGCY 2 cut(s) 296, 2145
BfrI CTTAAG 3 cut(s) 1304, 1966, 4190
BfuI GTATCC 2 cut(s) 3813, 4813
BglII AGATCT 3 cut(s) 1158, 2270, 2325
Bme1390I CCNGG 8 cut(s) 112, 124, 2408, 2547, 3095, 3350, 3819, 4764
Bme18I GGWCC 5 cut(s) 348, 767, 2374, 2543, 4760
BmeT110I CYCGRG 1 cut(s) 2682
BmgBI CACGTC 1 cut(s) 3418
BmgT120I GGNCC 7 cut(s) 348, 767, 2374, 2543, 3637, 3995, 4760
BmrFI CCNGG 8 cut(s) 112, 124, 2408, 2547, 3095, 3350, 3819, 4764
BmrI ACTGGG 2 cut(s) 400, 4732
BmtI GCTAGC 1 cut(s) 1515
BmuI ACTGGG 2 cut(s) 400, 4732
BpiI GAAGAC 5 cut(s) 714, 866, 1382, 2061, 2660
BpmI CTGGAG 5 cut(s) 597, 1431, 1734, 1785, 4127
Bpu10I CCTNAGC 6 cut(s) 690, 1089, 2138, 2781, 2898, 3045
Bpu14I TTCGAA 2 cut(s) 1766, 4326
BpuEI CTTGAG 4 cut(s) 1165, 2345, 3360, 4326
BpuMI CCSGG 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 2 cut(s) 1385, 2253
BsaHI GRCGYC 1 cut(s) 431
BsaJI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BsaWI WCCGGW 1 cut(s) 4658
BsaXI ACNNNNNCTCC 8 cut(s) 595, 625, 1707, 1737, 3033, 3063, 3747, 3777
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
Bse21I CCTNAGG 1 cut(s) 1683
Bse3DI GCAATG 3 cut(s) 488, 2050, 3630
Bse8I GATNNNNATC 2 cut(s) 1385, 2253
BseBI CCWGG 7 cut(s) 112, 2408, 2547, 3095, 3350, 3819, 4764
BseDI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BseGI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BseJI GATNNNNATC 2 cut(s) 1385, 2253
BseLI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
BseMI GCAATG 3 cut(s) 488, 2050, 3630
BseRI GAGGAG 5 cut(s) 71, 176, 2024, 3738, 4543
BseSI GKGCMC 2 cut(s) 1540, 4894
BseYI CCCAGC 1 cut(s) 4582
BsgI GTGCAG 2 cut(s) 3411, 4541
Bsh1236I CGCG 2 cut(s) 11, 237
BshFI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
BshNI GGYRCC 2 cut(s) 1961, 2290
BsiHKAI GWGCWC 3 cut(s) 91, 1540, 2438
BsiHKCI CYCGRG 1 cut(s) 2682
BsiSI CCGG 3 cut(s) 98, 124, 4659
BsiWI CGTACG 1 cut(s) 4850
BslFI GGGAC 4 cut(s) 361, 1467, 1778, 2627
BslI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
BsmAI GTCTC 3 cut(s) 1661, 3347, 3956
BsmFI GGGAC 4 cut(s) 361, 1467, 1778, 2627
BsmI GAATGC 1 cut(s) 3491
BsnI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
BsoBI CYCGRG 1 cut(s) 2682
Bsp119I TTCGAA 2 cut(s) 1766, 4326
Bsp1286I GDGCHC 4 cut(s) 91, 1540, 2438, 4894
Bsp1407I TGTACA 2 cut(s) 3697, 4483
BspANI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
BspFNI CGCG 2 cut(s) 11, 237
BspHI TCATGA 2 cut(s) 426, 4116
BspOI GCTAGC 1 cut(s) 1515
BspPI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
BspQI GCTCTTC 1 cut(s) 1620
BspT104I TTCGAA 2 cut(s) 1766, 4326
BspT107I GGYRCC 2 cut(s) 1961, 2290
BspTI CTTAAG 3 cut(s) 1304, 1966, 4190
BsrBI CCGCTC 1 cut(s) 3100
BsrDI GCAATG 3 cut(s) 488, 2050, 3630
BsrGI TGTACA 2 cut(s) 3697, 4483
BssECI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BssNAI GTATAC 1 cut(s) 3277
BssNI GRCGYC 1 cut(s) 431
BssSI CACGAG 2 cut(s) 221, 1527
BssT1I CCWWGG 1 cut(s) 4008
Bst1107I GTATAC 1 cut(s) 3277
Bst2BI CACGAG 2 cut(s) 221, 1527
Bst2UI CCWGG 7 cut(s) 112, 2408, 2547, 3095, 3350, 3819, 4764
Bst6I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
BstACI GRCGYC 1 cut(s) 431
BstAFI CTTAAG 3 cut(s) 1304, 1966, 4190
BstAPI GCANNNNNTGC 2 cut(s) 2827, 4877
BstAUI TGTACA 2 cut(s) 3697, 4483
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 2 cut(s) 1766, 4326
BstC8I GCNNGC 9 cut(s) 309, 1513, 2334, 2909, 3180, 3252, 3595, 3622, 4878
BstEII GGTNACC 1 cut(s) 3434
BstENI CCTNNNNNAGG 1 cut(s) 3929
BstF5I GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BstFNI CGCG 2 cut(s) 11, 237
BstH2I RGCGCY 2 cut(s) 296, 2145
BstHHI GCGC 5 cut(s) 13, 237, 283, 295, 2144
BstMAI GTCTC 3 cut(s) 1661, 3347, 3956
BstNI CCWGG 7 cut(s) 112, 2408, 2547, 3095, 3350, 3819, 4764
BstNSI RCATGY 5 cut(s) 1929, 3017, 3182, 3410, 4880
BstPI GGTNACC 1 cut(s) 3434
BstSCI CCNGG 8 cut(s) 110, 122, 2406, 2545, 3093, 3348, 3817, 4762
BstSFI CTRYAG 2 cut(s) 2185, 2698
BstSLI GKGCMC 2 cut(s) 1540, 4894
BstSNI TACGTA 1 cut(s) 449
BstUI CGCG 2 cut(s) 11, 237
BstV2I GAAGAC 5 cut(s) 714, 866, 1382, 2061, 2660
BstX2I RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
BstXI CCANNNNNNTGG 2 cut(s) 764, 4015
BstYI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
BstZ17I GTATAC 1 cut(s) 3277
Bsu36I CCTNAGG 1 cut(s) 1683
BsuI GTATCC 2 cut(s) 3813, 4813
BsuRI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
BtrI CACGTC 1 cut(s) 3418
BtsCI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BtsI GCAGTG 3 cut(s) 219, 3394, 4549
BtsIMutI CAGTG 9 cut(s) 219, 842, 1582, 1669, 3394, 3804, 4245, 4549, 4803
Cac8I GCNNGC 9 cut(s) 309, 1513, 2334, 2909, 3180, 3252, 3595, 3622, 4878
CaiI CAGNNNCTG 2 cut(s) 795, 1505
CciI TCATGA 2 cut(s) 426, 4116
CfoI GCGC 5 cut(s) 13, 237, 283, 295, 2144
Cfr13I GGNCC 7 cut(s) 348, 767, 2374, 2543, 3637, 3995, 4760
CseI GACGC 2 cut(s) 17, 439
Csp6I GTAC 8 cut(s) 888, 1110, 1887, 3698, 3921, 4484, 4851, 4900
CviQI GTAC 8 cut(s) 888, 1110, 1887, 3698, 3921, 4484, 4851, 4900
DraI TTTAAA 1 cut(s) 3514
EaeI YGGCCR 1 cut(s) 3820
Eam1104I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
EarI CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
Ecl136II GAGCTC 1 cut(s) 89
Eco105I TACGTA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 4008
Eco147I AGGCCT 1 cut(s) 4835
Eco24I GRGCYC 1 cut(s) 91
Eco47I GGWCC 5 cut(s) 348, 767, 2374, 2543, 4760
Eco47III AGCGCT 1 cut(s) 2143
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 5 cut(s) 1204, 1379, 1597, 2933, 3029
Eco81I CCTNAGG 1 cut(s) 1683
Eco88I CYCGRG 1 cut(s) 2682
Eco91I GGTNACC 1 cut(s) 3434
EcoICRI GAGCTC 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 3929
EcoO109I RGGNCCY 1 cut(s) 348
EcoO65I GGTNACC 1 cut(s) 3434
EcoRI GAATTC 2 cut(s) 1206, 1805
EcoRII CCWGG 7 cut(s) 110, 2406, 2545, 3093, 3348, 3817, 4762
EcoT14I CCWWGG 1 cut(s) 4008
EcoT22I ATGCAT 6 cut(s) 1012, 1247, 1875, 1927, 3590, 4183
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 4008
FalI AAGNNNNNCTT 6 cut(s) 315, 347, 2202, 2234, 4878, 4910
FaqI GGGAC 4 cut(s) 361, 1467, 1778, 2627
FauI CCCGC 1 cut(s) 16
FauNDI CATATG 1 cut(s) 266
FbaI TGATCA 2 cut(s) 3777, 4933
FblI GTMKAC 1 cut(s) 3276
FokI GGATG 6 cut(s) 522, 772, 1201, 1555, 1645, 3687
FriOI GRGCYC 1 cut(s) 91
GlaI GCGC 5 cut(s) 12, 236, 282, 294, 2143
GsaI CCCAGC 1 cut(s) 4586
GsuI CTGGAG 5 cut(s) 597, 1431, 1734, 1785, 4127
HaeII RGCGCY 2 cut(s) 296, 2145
HaeIII GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
HapII CCGG 3 cut(s) 98, 124, 4659
HgaI GACGC 2 cut(s) 17, 439
HhaI GCGC 5 cut(s) 13, 237, 283, 295, 2144
Hin1I GRCGYC 1 cut(s) 431
Hin6I GCGC 5 cut(s) 11, 235, 281, 293, 2142
HinP1I GCGC 5 cut(s) 11, 235, 281, 293, 2142
HincII GTYRAC 1 cut(s) 3502
HindII GTYRAC 1 cut(s) 3502
HindIII AAGCTT 2 cut(s) 1769, 3248
HpaII CCGG 3 cut(s) 98, 124, 4659
HphI GGTGA 9 cut(s) 92, 374, 1457, 1832, 2125, 2486, 3209, 3428, 3772
Hpy99I CGWCG 1 cut(s) 11
HpyCH4IV ACGT 8 cut(s) 448, 899, 1195, 3214, 3417, 3606, 4331, 4567
HpySE526I ACGT 8 cut(s) 448, 899, 1195, 3214, 3417, 3606, 4331, 4567
Hsp92I GRCGYC 1 cut(s) 431
HspAI GCGC 5 cut(s) 11, 235, 281, 293, 2142
Ksp22I TGATCA 2 cut(s) 3777, 4933
LguI GCTCTTC 1 cut(s) 1620
MaeII ACGT 8 cut(s) 448, 899, 1195, 3214, 3417, 3606, 4331, 4567
MbiI CCGCTC 1 cut(s) 3100
MfeI CAATTG 3 cut(s) 134, 3459, 3843
MflI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
MhlI GDGCHC 4 cut(s) 91, 1540, 2438, 4894
MlsI TGGCCA 1 cut(s) 3822
MluNI TGGCCA 1 cut(s) 3822
MlyI GAGTC 8 cut(s) 83, 796, 1058, 1353, 2018, 2021, 2114, 4040
MmeI TCCRAC 7 cut(s) 1575, 2100, 3136, 3466, 4152, 4383, 4533
Mox20I TGGCCA 1 cut(s) 3822
Mph1103I ATGCAT 6 cut(s) 1012, 1247, 1875, 1927, 3590, 4183
MroXI GAANNNNTTC 2 cut(s) 1210, 2400
MscI TGGCCA 1 cut(s) 3822
MslI CAYNNNNRTG 4 cut(s) 212, 269, 540, 762
Msp20I TGGCCA 1 cut(s) 3822
MspA1I CMGCKG 4 cut(s) 826, 1097, 3507, 4883
MspCI CTTAAG 3 cut(s) 1304, 1966, 4190
MspI CCGG 3 cut(s) 98, 124, 4659
MspR9I CCNGG 8 cut(s) 112, 124, 2408, 2547, 3095, 3350, 3819, 4764
MunI CAATTG 3 cut(s) 134, 3459, 3843
Mva1269I GAATGC 1 cut(s) 3491
MvaI CCWGG 7 cut(s) 112, 2408, 2547, 3095, 3350, 3819, 4764
MvnI CGCG 2 cut(s) 11, 237
NciI CCSGG 1 cut(s) 124
NdeI CATATG 1 cut(s) 266
NheI GCTAGC 1 cut(s) 1511
NmuCI GTSAC 9 cut(s) 866, 1524, 1723, 1970, 2131, 3344, 3402, 3413, 3434
NsiI ATGCAT 6 cut(s) 1012, 1247, 1875, 1927, 3590, 4183
NspI RCATGY 5 cut(s) 1929, 3017, 3182, 3410, 4880
NspV TTCGAA 2 cut(s) 1766, 4326
PaeI GCATGC 2 cut(s) 3182, 4880
PaeR7I CTCGAG 1 cut(s) 2682
PagI TCATGA 2 cut(s) 426, 4116
PasI CCCWGGG 1 cut(s) 2407
PceI AGGCCT 1 cut(s) 4835
PciSI GCTCTTC 1 cut(s) 1620
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 3491
PdmI GAANNNNTTC 2 cut(s) 1210, 2400
PfeI GAWTC 6 cut(s) 326, 1309, 1420, 2181, 2852, 3371
Pfl23II CGTACG 1 cut(s) 4850
PfoI TCCNGGA 1 cut(s) 2545
PleI GAGTC 8 cut(s) 82, 796, 1058, 1352, 2018, 2020, 2113, 4039
PpsI GAGTC 8 cut(s) 82, 796, 1058, 1352, 2018, 2020, 2113, 4039
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 348
PshBI ATTAAT 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 91
Psp1406I AACGTT 4 cut(s) 899, 3214, 4331, 4567
Psp5II RGGWCCY 1 cut(s) 348
Psp6I CCWGG 7 cut(s) 110, 2406, 2545, 3093, 3348, 3817, 4762
PspEI GGTNACC 1 cut(s) 3434
PspFI CCCAGC 1 cut(s) 4582
PspGI CCWGG 7 cut(s) 110, 2406, 2545, 3093, 3348, 3817, 4762
PspLI CGTACG 1 cut(s) 4850
PspPI GGNCC 7 cut(s) 348, 767, 2374, 2543, 3637, 3995, 4760
PspPPI RGGWCCY 1 cut(s) 348
PstNI CAGNNNCTG 2 cut(s) 795, 1505
PsuI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
PvuII CAGCTG 2 cut(s) 826, 1097
RsaI GTAC 8 cut(s) 889, 1111, 1888, 3699, 3922, 4485, 4852, 4901
RsaNI GTAC 8 cut(s) 888, 1110, 1887, 3698, 3921, 4484, 4851, 4900
RseI CAYNNNNRTG 4 cut(s) 212, 269, 540, 762
SacI GAGCTC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 1620
Sau96I GGNCC 7 cut(s) 348, 767, 2374, 2543, 3637, 3995, 4760
SchI GAGTC 8 cut(s) 83, 796, 1058, 1353, 2018, 2021, 2114, 4040
ScrFI CCNGG 8 cut(s) 112, 124, 2408, 2547, 3095, 3350, 3819, 4764
SduI GDGCHC 4 cut(s) 91, 1540, 2438, 4894
SfcI CTRYAG 2 cut(s) 2185, 2698
Sfr274I CTCGAG 1 cut(s) 2682
SfuI TTCGAA 2 cut(s) 1766, 4326
SinI GGWCC 5 cut(s) 348, 767, 2374, 2543, 4760
SlaI CTCGAG 1 cut(s) 2682
SmiMI CAYNNNNRTG 4 cut(s) 212, 269, 540, 762
SmlI CTYRAG 8 cut(s) 1180, 1304, 1966, 2360, 2682, 3375, 4190, 4341
SmoI CTYRAG 8 cut(s) 1180, 1304, 1966, 2360, 2682, 3375, 4190, 4341
SnaBI TACGTA 1 cut(s) 449
SphI GCATGC 2 cut(s) 3182, 4880
SseBI AGGCCT 1 cut(s) 4835
SspI AATATT 3 cut(s) 927, 2535, 3340
SstI GAGCTC 1 cut(s) 91
StuI AGGCCT 1 cut(s) 4835
StyD4I CCNGG 8 cut(s) 110, 122, 2406, 2545, 3093, 3348, 3817, 4762
StyI CCWWGG 1 cut(s) 4008
TaiI ACGT 8 cut(s) 451, 902, 1198, 3217, 3420, 3609, 4334, 4570
TaqII GACCGA 2 cut(s) 1395, 2391
TatI WGTACW 4 cut(s) 887, 1886, 3697, 4483
TauI GCSGC 4 cut(s) 16, 3327, 3521, 3638
TfiI GAWTC 6 cut(s) 326, 1309, 1420, 2181, 2852, 3371
TscAI CASTG 9 cut(s) 219, 842, 1582, 1669, 3394, 3804, 4245, 4549, 4803
TseFI GTSAC 9 cut(s) 866, 1524, 1723, 1970, 2131, 3344, 3402, 3413, 3434
Tsp45I GTSAC 9 cut(s) 866, 1524, 1723, 1970, 2131, 3344, 3402, 3413, 3434
TspGWI ACGGA 2 cut(s) 1555, 1711
TspRI CASTG 9 cut(s) 219, 842, 1582, 1669, 3394, 3804, 4245, 4549, 4803
Vha464I CTTAAG 3 cut(s) 1304, 1966, 4190
VneI GTGCAC 1 cut(s) 1536
VpaK11BI GGWCC 5 cut(s) 348, 767, 2374, 2543, 4760
VspI ATTAAT 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 3929
XbaI TCTAGA 2 cut(s) 809, 3067
XceI RCATGY 5 cut(s) 1929, 3017, 3182, 3410, 4880
XhoI CTCGAG 1 cut(s) 2682
XmiI GTMKAC 1 cut(s) 3276
XmnI GAANNNNTTC 2 cut(s) 1210, 2400
Zsp2I ATGCAT 6 cut(s) 1012, 1247, 1875, 1927, 3590, 4183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.