Rw2G033180
BHLH Family

Transcription factor bHLH68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001178
Physical Location & Seq
Reverse (-)
54357028 .. 54360226
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033180.1

Sequence Viewer

Length: 1026 bp
ATGATGGCCGGAAACCCTAACTGGTGGAGCACGCATCCACCGTCATCTATCTTCCCTTCTCAATATGTGCTTGGATCTTCTAATTCACTCCCTCAGCTCAATTCATTCCCTGATCAGCATAACCTACAGCCTCCACAATCTTGGAGCCAATTACTTCTGGGCGGATTGTCCGGAGAAGATCAAGACAACTTTGGAAGTTTGAATCATTTTCCACCTAAAAGGTTGGAAAATTGGGAGGATCATCAGCAGCAAATATTGATTCCACAGTCGTATCCAAGGGTTAATCCTGTTGTTGACAGTAATATTATAAAGCAAGAGGTTAATTCCCAGAACAACAGCAACTTGTGTGGTGGAGATGGAGGAGAAGAATTTCAGGCACCTTATAATAATAGGCCATCTTGGTCAGCAGCACAAATCAACATGCCAGTTTCATCCCCTAGGTCTTGTATCACTAGTTTAAGTAGTAATAATATCTTGGACTTTTCCTATAACCAGAAAAACCAACATGCAGATCACTCATCTGAGTGCAATAGTCCTCCCACCGACGGAATGTGCAAGAAAGCTAGGGTTCAACCATCTTCAAGCCAACCACCATTAAAGGTGAGAAAGGAGAAGCTAGGAGATAGAATAACAGCCCTTCATCAAATAGTTTCCCCATTTGGAAAGACTGACACTGCTTCTGTCTTGTTAGAAGCCATTGGGTATATCAGATTCCTCCAGAGTCAAATTGAGGCCCTCAGCTCCCCTTACTTGGGCAATGCCTCCAAAAACATGAGGAATAATCCCCACTCTGTTCAAGGAGAAAGGAATTGTGTGTTTCCTGAAGACCCTGGTCAGCTGTTGAATGACAACTGCAGCCTGAAAAGAAAAGGGGCTTCTAACCAGGATTCTCATCAAGACTATAAGGCAAAGGATTTAAGAAGTAAAGGATTGTGTTTGGTTCCTGTTTCTTGCACTCAGAATGTTGGGGGCGACATTAACATTGGAGCTGATTATTGGGCTCCGGCTTATGGGAGCGGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

37.53

Weight (kDa)

6.3

Isoelectric Point (pI)

63.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 308, 384
AccB1I GGYRCC 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 1017
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 2 cut(s) 162, 1017
AclWI GGATC 2 cut(s) 82, 246
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 843
AfiI CCNNNNNNNGG 4 cut(s) 545, 751, 752, 1010
AgsI TTSAA 5 cut(s) 202, 572, 582, 797, 844
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 2 cut(s) 829, 882
AluBI AGCT 6 cut(s) 97, 563, 616, 741, 838, 989
AluI AGCT 6 cut(s) 97, 563, 616, 741, 838, 989
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 2 cut(s) 82, 246
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 3 cut(s) 6, 392, 732
ApeKI GCWGC 3 cut(s) 247, 407, 855
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 369
AspA2I CCTAGG 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 733
AsuHPI GGTGA 1 cut(s) 613
AvrII CCTAGG 1 cut(s) 437
BaeI ACNNNNGTAYC 2 cut(s) 254, 287
BanI GGYRCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 1003
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 2 cut(s) 93, 737
BbvI GCAGC 3 cut(s) 259, 419, 867
BccI CCATC 3 cut(s) 350, 403, 583
BciT130I CCWGG 2 cut(s) 831, 884
BciVI GTATCC 1 cut(s) 282
BclI TGATCA 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 4 cut(s) 438, 453, 564, 617
BfmI CTRYAG 2 cut(s) 125, 853
BfuI GTATCC 1 cut(s) 282
BisI GCNGC 3 cut(s) 248, 408, 856
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 3 cut(s) 249, 409, 857
Bme1390I CCNGG 2 cut(s) 831, 884
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 4 cut(s) 146, 378, 942, 1002
BmrFI CCNGG 2 cut(s) 831, 884
BmsI GCATC 1 cut(s) 43
BoxI GACNNNNGTC 1 cut(s) 831
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 701
Bpu10I CCTNAGC 2 cut(s) 93, 737
BsaBI GATNNNNATC 1 cut(s) 891
BsaJI CCNNGG 3 cut(s) 275, 437, 829
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 4 cut(s) 545, 751, 752, 1010
Bse1I ACTGG 2 cut(s) 26, 425
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 891
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 2 cut(s) 831, 884
BseDI CCNNGG 3 cut(s) 275, 437, 829
BseGI GGATG 2 cut(s) 34, 431
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 4 cut(s) 545, 751, 752, 1010
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 4 cut(s) 107, 513, 751, 971
BseNI ACTGG 2 cut(s) 26, 425
BseRI GAGGAG 1 cut(s) 375
BseXI GCAGC 3 cut(s) 259, 419, 867
BshFI GGCC 3 cut(s) 8, 394, 734
BshNI GGYRCC 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 3 cut(s) 9, 171, 1004
BslI CCNNNNNNNGG 4 cut(s) 545, 751, 752, 1010
BsnI GGCC 3 cut(s) 8, 394, 734
Bsp1286I GDGCHC 2 cut(s) 32, 1003
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 5 cut(s) 74, 112, 178, 238, 511
BspACI CCGC 2 cut(s) 162, 1017
BspANI GGCC 3 cut(s) 8, 394, 734
BspCNI CTCAG 4 cut(s) 106, 514, 750, 970
BspEI TCCGGA 1 cut(s) 170
BspLI GGNNCC 4 cut(s) 146, 378, 942, 1002
BspMAI CTGCAG 1 cut(s) 857
BspPI GGATC 2 cut(s) 82, 246
BspT107I GGYRCC 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 1017
BsrDI GCAATG 1 cut(s) 763
BsrI ACTGG 2 cut(s) 26, 425
BssECI CCNNGG 3 cut(s) 275, 437, 829
BssMI GATC 5 cut(s) 74, 112, 178, 238, 511
BssT1I CCWWGG 2 cut(s) 275, 437
Bst2UI CCWGG 2 cut(s) 831, 884
Bst4CI ACNGT 3 cut(s) 42, 267, 299
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 4 cut(s) 93, 522, 737, 957
BstF5I GGATG 2 cut(s) 34, 431
BstKTI GATC 5 cut(s) 77, 115, 181, 241, 514
BstMBI GATC 5 cut(s) 74, 112, 178, 238, 511
BstNI CCWGG 2 cut(s) 831, 884
BstNSI RCATGY 2 cut(s) 424, 509
BstPAI GACNNNNGTC 1 cut(s) 831
BstSCI CCNGG 2 cut(s) 829, 882
BstSFI CTRYAG 2 cut(s) 125, 853
BstV1I GCAGC 3 cut(s) 259, 419, 867
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 74
BstXI CCANNNNNNTGG 1 cut(s) 141
BstYI RGATCY 1 cut(s) 74
BsuI GTATCC 1 cut(s) 282
BsuRI GGCC 3 cut(s) 8, 394, 734
BtsCI GGATG 2 cut(s) 34, 431
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 1 cut(s) 672
Cac8I GCNNGC 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 733
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 3 cut(s) 421, 506, 772
DdeI CTNAG 4 cut(s) 93, 522, 737, 957
DpnI GATC 5 cut(s) 76, 114, 180, 240, 513
DpnII GATC 5 cut(s) 74, 112, 178, 238, 511
EaeI YGGCCR 1 cut(s) 6
EciI GGCGGA 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 275, 437
Eco24I GRGCYC 1 cut(s) 1003
Eco57I CTGAAG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 733
EcoRII CCWGG 2 cut(s) 829, 882
EcoT14I CCWWGG 2 cut(s) 275, 437
EcoT38I GRGCYC 1 cut(s) 1003
ErhI CCWWGG 2 cut(s) 275, 437
FaeI CATG 3 cut(s) 424, 509, 775
FatI CATG 3 cut(s) 420, 505, 771
FauI CCCGC 1 cut(s) 1010
FbaI TGATCA 1 cut(s) 112
Fnu4HI GCNGC 3 cut(s) 248, 408, 856
FokI GGATG 2 cut(s) 21, 418
FriOI GRGCYC 1 cut(s) 1003
Fsp4HI GCNGC 3 cut(s) 248, 408, 856
FspBI CTAG 4 cut(s) 438, 453, 564, 617
GluI GCNGC 3 cut(s) 248, 408, 856
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 3 cut(s) 8, 394, 734
HapII CCGG 3 cut(s) 9, 171, 1004
Hin1II CATG 3 cut(s) 424, 509, 775
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 5 cut(s) 202, 259, 711, 721, 887
HpaII CCGG 3 cut(s) 9, 171, 1004
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 3 cut(s) 523, 710, 960
Hpy188III TCNNGA 5 cut(s) 171, 182, 718, 821, 896
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 2 cut(s) 66, 647
HpyCH4III ACNGT 3 cut(s) 42, 267, 299
HpyCH4V TGCA 5 cut(s) 509, 528, 555, 855, 954
HpyF3I CTNAG 4 cut(s) 93, 522, 737, 957
Hsp92II CATG 3 cut(s) 424, 509, 775
Kpn2I TCCGGA 1 cut(s) 170
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 5 cut(s) 74, 112, 178, 238, 511
LmnI GCTCC 6 cut(s) 27, 144, 746, 986, 1006, 1014
Lsp1109I GCAGC 3 cut(s) 259, 419, 867
LweI GCATC 1 cut(s) 43
MaeI CTAG 4 cut(s) 438, 453, 564, 617
MalI GATC 5 cut(s) 76, 114, 180, 240, 513
MbiI CCGCTC 1 cut(s) 1017
MboI GATC 5 cut(s) 74, 112, 178, 238, 511
MboII GAAGA 6 cut(s) 43, 69, 188, 377, 570, 836
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 2 cut(s) 32, 1003
MluCI AATT 8 cut(s) 82, 100, 149, 229, 322, 368, 726, 808
MlyI GAGTC 1 cut(s) 730
MmeI TCCRAC 1 cut(s) 204
MroI TCCGGA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 369
MseI TTAA 6 cut(s) 282, 321, 458, 596, 917, 978
MslI CAYNNNNRTG 1 cut(s) 523
MspA1I CMGCKG 1 cut(s) 838
MspI CCGG 3 cut(s) 9, 171, 1004
MspR9I CCNGG 2 cut(s) 831, 884
MvaI CCWGG 2 cut(s) 831, 884
NdeII GATC 5 cut(s) 74, 112, 178, 238, 511
NlaIII CATG 3 cut(s) 424, 509, 775
NlaIV GGNNCC 4 cut(s) 146, 378, 942, 1002
NspI RCATGY 2 cut(s) 424, 509
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 4 cut(s) 202, 259, 711, 887
PkrI GCNGC 3 cut(s) 249, 409, 857
PleI GAGTC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 729
PshAI GACNNNNGTC 1 cut(s) 831
PsiI TTATAA 2 cut(s) 308, 384
Psp6I CCWGG 2 cut(s) 829, 882
PspGI CCWGG 2 cut(s) 829, 882
PspN4I GGNNCC 4 cut(s) 146, 378, 942, 1002
PspPI GGNCC 1 cut(s) 733
PstI CTGCAG 1 cut(s) 857
PsuI RGATCY 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 838
RseI CAYNNNNRTG 1 cut(s) 523
SaqAI TTAA 6 cut(s) 282, 321, 458, 596, 917, 978
SatI GCNGC 3 cut(s) 248, 408, 856
Sau3AI GATC 5 cut(s) 74, 112, 178, 238, 511
Sau96I GGNCC 1 cut(s) 733
SchI GAGTC 1 cut(s) 730
ScrFI CCNGG 2 cut(s) 831, 884
SduI GDGCHC 2 cut(s) 32, 1003
SfaNI GCATC 1 cut(s) 43
SfcI CTRYAG 2 cut(s) 125, 853
SmiMI CAYNNNNRTG 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 8 cut(s) 82, 100, 149, 229, 322, 368, 726, 808
SsiI CCGC 2 cut(s) 162, 1017
SspI AATATT 2 cut(s) 255, 304
SspMI CTAG 4 cut(s) 438, 453, 564, 617
StyD4I CCNGG 2 cut(s) 829, 882
StyI CCWWGG 2 cut(s) 275, 437
TaaI ACNGT 3 cut(s) 42, 267, 299
TasI AATT 8 cut(s) 82, 100, 149, 229, 322, 368, 726, 808
TfiI GAWTC 4 cut(s) 202, 259, 711, 887
Tru1I TTAA 6 cut(s) 282, 321, 458, 596, 917, 978
Tru9I TTAA 6 cut(s) 282, 321, 458, 596, 917, 978
TscAI CASTG 1 cut(s) 679
TseI GCWGC 3 cut(s) 247, 407, 855
TspDTI ATGAA 3 cut(s) 93, 420, 629
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 1 cut(s) 679
XapI RAATTY 1 cut(s) 368
XceI RCATGY 2 cut(s) 424, 509
XmaJI CCTAGG 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 4 cut(s) 438, 453, 564, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.