Rroxscaffold_2G00105520
BHLH Family

Transcription factor bHLH68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001178
Physical Location & Seq
Forward (+)
28928428 .. 28931741
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105520.1

Sequence Viewer

Length: 1029 bp
ATGATGGCCGGAAACCCTAACTGGTGGAGCACGCATCCACCGTCATCTATCTTCCCTTCTCAATATGTGCTTGGATCTTCTAATTCACTCCCTCAGCTCAATTCCTTCCCTGATCAGCATAACCTACAGCCTCCTCAGTCTTGGAGCCAATTACTTCTGGGCGGATTGTCCGGAGAAGATCAAGACAACTTTGGAAGTTTGAATCATTTTCCACCTAAAAGGTTGGAAAGTTGGGAGGATCATCAGCAGCAAATATTGATTCCACAGTCGTATCCAAGGATTAATCCTGTTGTTGATAGTAATATTATAAAGCAAGAGGTTAATTACCAGAACAACAGCAACTTGTGTGGTGGAGATGGAGGAGAAGAATTTCAGGCACCTTATAATAATAGGCCATCTTGGTCAGCAGCACAAATCAACATGCCAGTTTCATCCCCTAGGTCTTGTATCACTAGTTTAAGTAGTAATAATATATTGGACTTTTCCTATAACCAGAAGAACCAACATGCAGATCACTCATCTGAGTGCAATAGTCCTCCCATCGGCGGAATGTGCAAGAAAGCTAGGGTTCAACCATCTTCAAGCCAACCACCATTAAAGGTGAGAAAGGAGAAGCTAGGAGATAGAATAACAGCCCTTCATCAAATAGTTTCCCCATTTGGAAAGACTGACACTGCTTCTGTCTTGTTAGAAGCCATTGGGTATATCAGATTCCTCCAGAGTCAAATTGAGGCCCTCAGCTCCCCTTACTTGGGCAATGCCTCCAAAAACATGAGGAATAATCCCCACTCTGTTCAAGGAGAAAGGAATTGTGTGTTTCCTGAAGACCCTGGTCAGCTGTTGAATGACAACTGCAGCCTGAAAAGAAAAGGGGCTTCTAACCAGCAGGATTCTCATCAAGACTATAAGGCAAAGGATTTAAGAAGTAAAGGATTGTGTTTGGTTCCTGTTTCTTGCACTCAGAATGTTGGGGGTGACATTAACATTGGAGCTGATTATTGGGCTCCGGCTTATGGGAGCGGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

37.68

Weight (kDa)

6.5

Isoelectric Point (pI)

64.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 308, 384
AccB1I GGYRCC 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 1020
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 3 cut(s) 162, 546, 1020
AclWI GGATC 2 cut(s) 82, 246
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 843
AfiI CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 1013
AgsI TTSAA 5 cut(s) 202, 572, 582, 797, 844
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 1 cut(s) 829
AluBI AGCT 6 cut(s) 97, 563, 616, 741, 838, 992
AluI AGCT 6 cut(s) 97, 563, 616, 741, 838, 992
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 2 cut(s) 82, 246
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 3 cut(s) 6, 392, 732
ApeKI GCWGC 3 cut(s) 247, 407, 855
ApoI RAATTY 1 cut(s) 368
AseI ATTAAT 1 cut(s) 282
Asp700I GAANNNNTTC 1 cut(s) 369
AspA2I CCTAGG 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 733
AsuHPI GGTGA 2 cut(s) 613, 986
AvrII CCTAGG 1 cut(s) 437
BaeI ACNNNNGTAYC 2 cut(s) 254, 287
BanI GGYRCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 2 cut(s) 93, 737
BbvI GCAGC 3 cut(s) 259, 419, 867
BccI CCATC 4 cut(s) 350, 403, 548, 583
BciT130I CCWGG 1 cut(s) 831
BciVI GTATCC 1 cut(s) 282
BclI TGATCA 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 4 cut(s) 438, 453, 564, 617
BfmI CTRYAG 2 cut(s) 125, 853
BfuI GTATCC 1 cut(s) 282
BisI GCNGC 3 cut(s) 248, 408, 856
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 3 cut(s) 249, 409, 857
Bme1390I CCNGG 1 cut(s) 831
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 4 cut(s) 146, 378, 945, 1005
BmrFI CCNGG 1 cut(s) 831
BmsI GCATC 1 cut(s) 43
BoxI GACNNNNGTC 1 cut(s) 831
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 701
Bpu10I CCTNAGC 2 cut(s) 93, 737
BsaBI GATNNNNATC 1 cut(s) 894
BsaJI CCNNGG 3 cut(s) 275, 437, 829
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 1013
Bse1I ACTGG 2 cut(s) 26, 425
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 894
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 1 cut(s) 831
BseDI CCNNGG 3 cut(s) 275, 437, 829
BseGI GGATG 2 cut(s) 34, 431
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 1013
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 5 cut(s) 107, 149, 513, 751, 974
BseNI ACTGG 2 cut(s) 26, 425
BseRI GAGGAG 2 cut(s) 123, 375
BseXI GCAGC 3 cut(s) 259, 419, 867
BshFI GGCC 3 cut(s) 8, 394, 734
BshNI GGYRCC 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 3 cut(s) 9, 171, 1007
BslI CCNNNNNNNGG 5 cut(s) 542, 545, 751, 752, 1013
BsnI GGCC 3 cut(s) 8, 394, 734
Bsp1286I GDGCHC 2 cut(s) 32, 1006
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 5 cut(s) 74, 112, 178, 238, 511
BspACI CCGC 3 cut(s) 162, 546, 1020
BspANI GGCC 3 cut(s) 8, 394, 734
BspCNI CTCAG 5 cut(s) 106, 148, 514, 750, 973
BspEI TCCGGA 1 cut(s) 170
BspLI GGNNCC 4 cut(s) 146, 378, 945, 1005
BspMAI CTGCAG 1 cut(s) 857
BspPI GGATC 2 cut(s) 82, 246
BspT107I GGYRCC 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 1020
BsrDI GCAATG 1 cut(s) 763
BsrI ACTGG 2 cut(s) 26, 425
BssECI CCNNGG 3 cut(s) 275, 437, 829
BssMI GATC 5 cut(s) 74, 112, 178, 238, 511
BssT1I CCWWGG 2 cut(s) 275, 437
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 2 cut(s) 42, 267
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 5 cut(s) 93, 135, 522, 737, 960
BstF5I GGATG 2 cut(s) 34, 431
BstKTI GATC 5 cut(s) 77, 115, 181, 241, 514
BstMBI GATC 5 cut(s) 74, 112, 178, 238, 511
BstMWI GCNNNNNNNGC 1 cut(s) 552
BstNI CCWGG 1 cut(s) 831
BstNSI RCATGY 2 cut(s) 424, 509
BstPAI GACNNNNGTC 1 cut(s) 831
BstSCI CCNGG 1 cut(s) 829
BstSFI CTRYAG 2 cut(s) 125, 853
BstV1I GCAGC 3 cut(s) 259, 419, 867
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
BsuI GTATCC 1 cut(s) 282
BsuRI GGCC 3 cut(s) 8, 394, 734
BtsCI GGATG 2 cut(s) 34, 431
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 1 cut(s) 672
Cac8I GCNNGC 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 733
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 3 cut(s) 421, 506, 772
DdeI CTNAG 5 cut(s) 93, 135, 522, 737, 960
DpnI GATC 5 cut(s) 76, 114, 180, 240, 513
DpnII GATC 5 cut(s) 74, 112, 178, 238, 511
EaeI YGGCCR 1 cut(s) 6
EciI GGCGGA 2 cut(s) 177, 561
Eco130I CCWWGG 2 cut(s) 275, 437
Eco24I GRGCYC 1 cut(s) 1006
Eco57I CTGAAG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 733
EcoRII CCWGG 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 275, 437
EcoT38I GRGCYC 1 cut(s) 1006
ErhI CCWWGG 2 cut(s) 275, 437
FaeI CATG 3 cut(s) 424, 509, 775
FatI CATG 3 cut(s) 420, 505, 771
FauI CCCGC 1 cut(s) 1013
FbaI TGATCA 1 cut(s) 112
Fnu4HI GCNGC 3 cut(s) 248, 408, 856
FokI GGATG 2 cut(s) 21, 418
FriOI GRGCYC 1 cut(s) 1006
Fsp4HI GCNGC 3 cut(s) 248, 408, 856
FspBI CTAG 4 cut(s) 438, 453, 564, 617
GluI GCNGC 3 cut(s) 248, 408, 856
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 3 cut(s) 8, 394, 734
HapII CCGG 3 cut(s) 9, 171, 1007
Hin1II CATG 3 cut(s) 424, 509, 775
HinfI GANTC 5 cut(s) 202, 259, 711, 721, 890
HpaII CCGG 3 cut(s) 9, 171, 1007
HphI GGTGA 2 cut(s) 613, 986
Hpy188I TCNGA 3 cut(s) 523, 710, 963
Hpy188III TCNNGA 5 cut(s) 171, 182, 718, 821, 899
HpyAV CCTTC 3 cut(s) 66, 115, 647
HpyCH4III ACNGT 2 cut(s) 42, 267
HpyCH4V TGCA 5 cut(s) 509, 528, 555, 855, 957
HpyF10VI GCNNNNNNNGC 1 cut(s) 552
HpyF3I CTNAG 5 cut(s) 93, 135, 522, 737, 960
Hsp92II CATG 3 cut(s) 424, 509, 775
Kpn2I TCCGGA 1 cut(s) 170
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 5 cut(s) 74, 112, 178, 238, 511
LmnI GCTCC 6 cut(s) 27, 144, 746, 989, 1009, 1017
Lsp1109I GCAGC 3 cut(s) 259, 419, 867
LweI GCATC 1 cut(s) 43
MaeI CTAG 4 cut(s) 438, 453, 564, 617
MaeIII GTNAC 1 cut(s) 974
MalI GATC 5 cut(s) 76, 114, 180, 240, 513
MbiI CCGCTC 1 cut(s) 1020
MboI GATC 5 cut(s) 74, 112, 178, 238, 511
MboII GAAGA 7 cut(s) 43, 69, 188, 377, 508, 570, 836
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 2 cut(s) 32, 1006
MluCI AATT 7 cut(s) 82, 100, 149, 322, 368, 726, 808
MlyI GAGTC 1 cut(s) 730
MmeI TCCRAC 1 cut(s) 204
MroI TCCGGA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 369
MseI TTAA 6 cut(s) 282, 321, 458, 596, 920, 981
MslI CAYNNNNRTG 1 cut(s) 523
MspA1I CMGCKG 1 cut(s) 838
MspI CCGG 3 cut(s) 9, 171, 1007
MspR9I CCNGG 1 cut(s) 831
MvaI CCWGG 1 cut(s) 831
MwoI GCNNNNNNNGC 1 cut(s) 552
NdeII GATC 5 cut(s) 74, 112, 178, 238, 511
NlaIII CATG 3 cut(s) 424, 509, 775
NlaIV GGNNCC 4 cut(s) 146, 378, 945, 1005
NmuCI GTSAC 1 cut(s) 974
NspI RCATGY 2 cut(s) 424, 509
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 4 cut(s) 202, 259, 711, 890
PkrI GCNGC 3 cut(s) 249, 409, 857
PleI GAGTC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 729
PshAI GACNNNNGTC 1 cut(s) 831
PshBI ATTAAT 1 cut(s) 282
PsiI TTATAA 2 cut(s) 308, 384
Psp6I CCWGG 1 cut(s) 829
PspGI CCWGG 1 cut(s) 829
PspN4I GGNNCC 4 cut(s) 146, 378, 945, 1005
PspPI GGNCC 1 cut(s) 733
PstI CTGCAG 1 cut(s) 857
PsuI RGATCY 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 838
RseI CAYNNNNRTG 1 cut(s) 523
SaqAI TTAA 6 cut(s) 282, 321, 458, 596, 920, 981
SatI GCNGC 3 cut(s) 248, 408, 856
Sau3AI GATC 5 cut(s) 74, 112, 178, 238, 511
Sau96I GGNCC 1 cut(s) 733
SchI GAGTC 1 cut(s) 730
ScrFI CCNGG 1 cut(s) 831
SduI GDGCHC 2 cut(s) 32, 1006
SfaNI GCATC 1 cut(s) 43
SfcI CTRYAG 2 cut(s) 125, 853
SmiMI CAYNNNNRTG 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 7 cut(s) 82, 100, 149, 322, 368, 726, 808
SsiI CCGC 3 cut(s) 162, 546, 1020
SspI AATATT 2 cut(s) 255, 304
SspMI CTAG 4 cut(s) 438, 453, 564, 617
StyD4I CCNGG 1 cut(s) 829
StyI CCWWGG 2 cut(s) 275, 437
TaaI ACNGT 2 cut(s) 42, 267
TasI AATT 7 cut(s) 82, 100, 149, 322, 368, 726, 808
TfiI GAWTC 4 cut(s) 202, 259, 711, 890
Tru1I TTAA 6 cut(s) 282, 321, 458, 596, 920, 981
Tru9I TTAA 6 cut(s) 282, 321, 458, 596, 920, 981
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 1 cut(s) 974
TseI GCWGC 3 cut(s) 247, 407, 855
Tsp45I GTSAC 1 cut(s) 974
TspDTI ATGAA 2 cut(s) 420, 629
TspRI CASTG 1 cut(s) 679
VspI ATTAAT 1 cut(s) 282
XapI RAATTY 1 cut(s) 368
XceI RCATGY 2 cut(s) 424, 509
XmaJI CCTAGG 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 4 cut(s) 438, 453, 564, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.