MD02G1164500.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005167
Physical Location & Seq
Forward (+)
14047028 .. 14047783
756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1164500.v1.1.491

Sequence Viewer

Length: 549 bp
ATGTGTAATATGGGTCAAGCTTTCCACAATTGTTCCATCAAAATTTCTGATGCCTGGCCAGGTCTAGAGGAAATGAATATCGACTATTGCGATGATTTGGTGGAACTGCCTGCCGAGCTCTGTGATCTTGTTCATATGAAGGTGCTTAGTATCACCAACTGCCATCAGCTATCTTGCTTGCCTGAAGAGATCGGAAATCTGAAGAAATTGGATGTATTGAGACTAAGGTCCTGTACAGAGTTGGTAAGGCTTCCGGTTTCAATTGAAAACCTCACTAAGTTGCGCTGCCTTGACATGTATAATTGTTTCGGCATTAGGAAGTTGCCTGAAGGCATTGGTAAGATGAGAGGTTTAAGAAAGATCAACATGGGACAATGCTCAAGATTGGAGGAGTTGCCTCTATCAGTGTGGGATCTTGACGAGCAGTTAGAGGAAGTGATTTGTGATGAAGAGAAGAAACCTTTCTGGGATTCCTTCTTAGCCAGAGACAAAATCAAGGTGACAAAAGAAAAATCCAGTCTGAATTGGCTCGAAAAGCCTCAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

183

Amino Acids

21.03

Weight (kDa)

5.21

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 38 - 126 5.3e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 420
AcoI YGGCCR 1 cut(s) 56
AcsI RAATTY 1 cut(s) 42
AcuI CTGAAG 3 cut(s) 204, 221, 348
AfaI GTAC 1 cut(s) 235
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 2 cut(s) 261, 266
AjnI CCWGG 2 cut(s) 53, 58
AluBI AGCT 3 cut(s) 20, 118, 169
AluI AGCT 3 cut(s) 20, 118, 169
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 2 cut(s) 214, 480
AlwI GGATC 1 cut(s) 420
AoxI GGCC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 285
ApoI RAATTY 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 145, 511
AvaII GGWCC 1 cut(s) 228
BalI TGGCCA 1 cut(s) 58
BanII GRGCYC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 272
BccI CCATC 2 cut(s) 44, 171
BciT130I CCWGG 2 cut(s) 55, 60
BcoDI GTCTC 2 cut(s) 214, 480
BfaI CTAG 1 cut(s) 65
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
Bme1390I CCNGG 2 cut(s) 55, 60
Bme18I GGWCC 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 228
BmrFI CCNGG 2 cut(s) 55, 60
BmsI GCATC 1 cut(s) 40
BoxI GACNNNNGTC 1 cut(s) 226
BpuEI CTTGAG 1 cut(s) 364
BsaWI WCCGGW 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 516
BseBI CCWGG 2 cut(s) 55, 60
BseGI GGATG 1 cut(s) 217
BseNI ACTGG 1 cut(s) 516
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 272
BshFI GGCC 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 384
BsmAI GTCTC 2 cut(s) 214, 480
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 1 cut(s) 58
Bsp1286I GDGCHC 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 233
Bsp143I GATC 4 cut(s) 124, 189, 360, 412
BspANI GGCC 1 cut(s) 58
BspPI GGATC 1 cut(s) 420
BsrGI TGTACA 1 cut(s) 233
BsrI ACTGG 1 cut(s) 516
BssMI GATC 4 cut(s) 124, 189, 360, 412
Bst2UI CCWGG 2 cut(s) 55, 60
Bst6I CTCTTC 2 cut(s) 180, 444
BstAUI TGTACA 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 111, 179
BstDEI CTNAG 5 cut(s) 146, 224, 276, 478, 540
BstF5I GGATG 1 cut(s) 217
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 4 cut(s) 127, 192, 363, 415
BstMAI GTCTC 2 cut(s) 214, 480
BstMBI GATC 4 cut(s) 124, 189, 360, 412
BstMWI GCNNNNNNNGC 2 cut(s) 115, 535
BstNI CCWGG 2 cut(s) 55, 60
BstNSI RCATGY 1 cut(s) 298
BstPAI GACNNNNGTC 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 53, 58
BstV1I GCAGC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuRI GGCC 1 cut(s) 58
BtgZI GCGATG 1 cut(s) 105
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 111, 179
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 234
CviAII CATG 2 cut(s) 295, 367
CviJI RGCY 8 cut(s) 20, 58, 118, 169, 250, 482, 529, 538
CviKI_1 RGCY 8 cut(s) 20, 58, 118, 169, 250, 482, 529, 538
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 5 cut(s) 146, 224, 276, 478, 540
DpnI GATC 4 cut(s) 126, 191, 362, 414
DpnII GATC 4 cut(s) 124, 189, 360, 412
EaeI YGGCCR 1 cut(s) 56
Eam1104I CTCTTC 2 cut(s) 180, 444
EarI CTCTTC 2 cut(s) 180, 444
Ecl136II GAGCTC 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 3 cut(s) 204, 221, 348
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 53, 58
EcoT38I GRGCYC 1 cut(s) 120
FaeI CATG 2 cut(s) 298, 370
FaiI YATR 7 cut(s) 11, 135, 137, 296, 300, 368, 547
FaqI GGGAC 1 cut(s) 384
FatI CATG 2 cut(s) 294, 366
FauNDI CATATG 1 cut(s) 135
Fnu4HI GCNGC 1 cut(s) 286
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 286
FspBI CTAG 1 cut(s) 65
GlaI GCGC 1 cut(s) 284
GluI GCNGC 1 cut(s) 286
HaeIII GGCC 1 cut(s) 58
HapII CCGG 1 cut(s) 254
HhaI GCGC 1 cut(s) 285
Hin1II CATG 2 cut(s) 298, 370
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 1 cut(s) 470
HpaII CCGG 1 cut(s) 254
HphI GGTGA 2 cut(s) 145, 511
Hpy188I TCNGA 4 cut(s) 49, 194, 201, 522
Hpy188III TCNNGA 3 cut(s) 65, 381, 416
HpyAV CCTTC 3 cut(s) 133, 323, 484
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 535
HpyF3I CTNAG 5 cut(s) 146, 224, 276, 478, 540
Hsp92II CATG 2 cut(s) 298, 370
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 4 cut(s) 124, 189, 360, 412
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 1 cut(s) 40
MaeI CTAG 1 cut(s) 65
MaeIII GTNAC 1 cut(s) 499
MalI GATC 4 cut(s) 126, 191, 362, 414
MboI GATC 4 cut(s) 124, 189, 360, 412
MboII GAAGA 4 cut(s) 197, 214, 461, 466
MfeI CAATTG 2 cut(s) 28, 261
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 120
MlsI TGGCCA 1 cut(s) 58
MluCI AATT 6 cut(s) 28, 42, 206, 261, 301, 523
MluNI TGGCCA 1 cut(s) 58
MnlI CCTC 7 cut(s) 61, 281, 341, 382, 408, 424, 549
Mox20I TGGCCA 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 461
MscI TGGCCA 1 cut(s) 58
MseI TTAA 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 58
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 55, 60
MunI CAATTG 2 cut(s) 28, 261
MvaI CCWGG 2 cut(s) 55, 60
MwoI GCNNNNNNNGC 2 cut(s) 115, 535
NdeI CATATG 1 cut(s) 135
NdeII GATC 4 cut(s) 124, 189, 360, 412
NlaIII CATG 2 cut(s) 298, 370
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 499
NspI RCATGY 1 cut(s) 298
PciI ACATGT 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 1 cut(s) 287
PpuMI RGGWCCY 1 cut(s) 228
PscI ACATGT 1 cut(s) 294
PshAI GACNNNNGTC 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 53, 58
PspGI CCWGG 2 cut(s) 53, 58
PspPI GGNCC 1 cut(s) 228
PspPPI RGGWCCY 1 cut(s) 228
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 4 cut(s) 124, 189, 360, 412
Sau96I GGNCC 1 cut(s) 228
ScrFI CCNGG 2 cut(s) 55, 60
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 1 cut(s) 40
SinI GGWCC 1 cut(s) 228
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 6 cut(s) 28, 42, 206, 261, 301, 523
SspMI CTAG 1 cut(s) 65
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 53, 58
TaqI TCGA 2 cut(s) 81, 531
TasI AATT 6 cut(s) 28, 42, 206, 261, 301, 523
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 1 cut(s) 285
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 4 cut(s) 89, 122, 152, 462
TspRI CASTG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 228
XapI RAATTY 1 cut(s) 42
XbaI TCTAGA 1 cut(s) 64
XceI RCATGY 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.