MD02G1165200.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005167
Physical Location & Seq
Forward (+)
14112374 .. 14112823
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1165200.v1.1.491

Sequence Viewer

Length: 450 bp
ATGAATATCGACTATTGCCATGATTTGGTGGAACTGCCTGCCGAGCTCTGTGATCTTGTTCATATGAAGATGCTCAGTATCACCAACTGCCATAAGTTATCTGCCTTGCCTGAAGAGATCGGAAAGCTGGAGAATTTGGATGTACTGCGACTAAGGTCCTGTACAGACTTGGTAAGGCTTCCGGTCTCGATTGAGAACCTTGCTAAGTTGTGCTGTCTTGACATGTATGATTGTTTTGGCATTAGGAAGTTGCCTGAAGGCATTGGTAATATGAGCGGTTTAAGAAAGATCTACATGGGACAATGCTCAAGATTGAAAGAGTTGCCTCTGTCAGCGTGGGATCTTGACGAGCAGTTAGAGGAAGTGATTTGTGATGAAGAGAAGAAACATTTGTGGGAATCCTTCTTAGCCAGAGACAAAATCAAGGTGGCAAAAGAAAAACCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

150

Amino Acids

17.13

Weight (kDa)

5.26

Isoelectric Point (pI)

62.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 14 - 102 1.2e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 276
AciI CCGC 1 cut(s) 276
AclWI GGATC 1 cut(s) 348
AcsI RAATTY 1 cut(s) 133
AcuI CTGAAG 2 cut(s) 132, 276
AfaI GTAC 2 cut(s) 144, 163
AfiI CCNNNNNNNGG 1 cut(s) 25
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 1 cut(s) 316
AluBI AGCT 2 cut(s) 46, 127
AluI AGCT 2 cut(s) 46, 127
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 2 cut(s) 190, 408
AlwI GGATC 1 cut(s) 348
ApoI RAATTY 1 cut(s) 133
AspS9I GGNCC 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 73
AvaII GGWCC 1 cut(s) 156
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 48
BcoDI GTCTC 2 cut(s) 190, 408
BglII AGATCT 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 156
BmgT120I GGNCC 1 cut(s) 156
BmsI GCATC 1 cut(s) 60
BoxI GACNNNNGTC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 292
BsaI GGTCTC 1 cut(s) 190
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 25
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 1 cut(s) 25
BsmAI GTCTC 2 cut(s) 190, 408
BsmFI GGGAC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 48
Bsp1407I TGTACA 1 cut(s) 161
Bsp143I GATC 4 cut(s) 52, 117, 288, 340
BspACI CCGC 1 cut(s) 276
BspCNI CTCAG 1 cut(s) 87
BspPI GGATC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 190
BsrBI CCGCTC 1 cut(s) 276
BsrGI TGTACA 1 cut(s) 161
BssMI GATC 4 cut(s) 52, 117, 288, 340
Bst6I CTCTTC 2 cut(s) 108, 372
BstAUI TGTACA 1 cut(s) 161
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 4 cut(s) 74, 152, 204, 406
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 4 cut(s) 55, 120, 291, 343
BstMAI GTCTC 2 cut(s) 190, 408
BstMBI GATC 4 cut(s) 52, 117, 288, 340
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 226
BstPAI GACNNNNGTC 1 cut(s) 154
BstX2I RGATCY 2 cut(s) 288, 340
BstYI RGATCY 2 cut(s) 288, 340
BtsCI GGATG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 156
Csp6I GTAC 2 cut(s) 143, 162
CviAII CATG 3 cut(s) 20, 223, 295
CviJI RGCY 4 cut(s) 46, 127, 178, 410
CviKI_1 RGCY 4 cut(s) 46, 127, 178, 410
CviQI GTAC 2 cut(s) 143, 162
DdeI CTNAG 4 cut(s) 74, 152, 204, 406
DpnI GATC 4 cut(s) 54, 119, 290, 342
DpnII GATC 4 cut(s) 52, 117, 288, 340
Eam1104I CTCTTC 2 cut(s) 108, 372
EarI CTCTTC 2 cut(s) 108, 372
Ecl136II GAGCTC 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 132, 276
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 156
EcoT38I GRGCYC 1 cut(s) 48
FaeI CATG 3 cut(s) 23, 226, 298
FaiI YATR 8 cut(s) 21, 63, 65, 93, 224, 228, 272, 296
FaqI GGGAC 1 cut(s) 312
FatI CATG 3 cut(s) 19, 222, 294
FauNDI CATATG 1 cut(s) 63
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 48
GsuI CTGGAG 1 cut(s) 149
HapII CCGG 1 cut(s) 182
Hin1II CATG 3 cut(s) 23, 226, 298
HinfI GANTC 1 cut(s) 398
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 122, 449
Hpy188III TCNNGA 4 cut(s) 187, 218, 309, 344
HpyAV CCTTC 2 cut(s) 251, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 4 cut(s) 74, 152, 204, 406
Hsp92II CATG 3 cut(s) 23, 226, 298
Kzo9I GATC 4 cut(s) 52, 117, 288, 340
LpnPI CCDG 7 cut(s) 51, 113, 123, 172, 195, 267, 424
LweI GCATC 1 cut(s) 60
MalI GATC 4 cut(s) 54, 119, 290, 342
MbiI CCGCTC 1 cut(s) 276
MboI GATC 4 cut(s) 52, 117, 288, 340
MboII GAAGA 4 cut(s) 79, 125, 389, 394
MflI RGATCY 2 cut(s) 288, 340
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 1 cut(s) 133
MnlI CCTC 2 cut(s) 336, 352
MseI TTAA 1 cut(s) 281
MspI CCGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 43
NdeI CATATG 1 cut(s) 63
NdeII GATC 4 cut(s) 52, 117, 288, 340
NlaIII CATG 3 cut(s) 23, 226, 298
NmeAIII GCCGAG 1 cut(s) 67
NspI RCATGY 1 cut(s) 226
PciI ACATGT 1 cut(s) 222
PfeI GAWTC 1 cut(s) 398
PflMI CCANNNNNTGG 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 156
PscI ACATGT 1 cut(s) 222
PshAI GACNNNNGTC 1 cut(s) 154
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 156
PspPI GGNCC 1 cut(s) 156
PspPPI RGGWCCY 1 cut(s) 156
PsuI RGATCY 2 cut(s) 288, 340
RsaI GTAC 2 cut(s) 144, 163
RsaNI GTAC 2 cut(s) 143, 162
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 281
Sau3AI GATC 4 cut(s) 52, 117, 288, 340
Sau96I GGNCC 1 cut(s) 156
SduI GDGCHC 1 cut(s) 48
SetI ASST 5 cut(s) 48, 129, 158, 201, 429
SfaNI GCATC 1 cut(s) 60
SinI GGWCC 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 1 cut(s) 133
SsiI CCGC 1 cut(s) 276
SstI GAGCTC 1 cut(s) 48
TaqI TCGA 2 cut(s) 9, 188
TasI AATT 1 cut(s) 133
TatI WGTACW 2 cut(s) 142, 161
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TspDTI ATGAA 4 cut(s) 17, 50, 80, 390
Van91I CCANNNNNTGG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 1 cut(s) 133
XceI RCATGY 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.