RchiOBHm_Chr2g0104341

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005167
Physical Location & Seq
Forward (+)
15725328 .. 15726285
958 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47861

Sequence Viewer

Length: 630 bp
ATGAGATTAGAACGCATCTCAATTCCATCCATAGCCAAGCACCCCATGCAATTGAATGGTCTCCAGAAGATTTCTTTATTCATGTGTAAAATTGGTCAAGCTTTCAGCAATGGGTCAATCCAATTTTCACATGCATGGCCAAATCTAGTGGAAGTGAACATTGACTATTGCAATGATTTGGTGGCATTGCCTGCTGACACCAGTGATTTGATTCTTCTAGAGAAGCTCAGCATCACCAACTGTCATAAGCTACTTGCCTTGCCTGAAGATATTGGAAAGCTGGCCAAATTAGAATTGTTGAGGCTAAGGTCTTGTACAGGCTTAGCAAAGTTGCCGGGTTCGACTGGGAACCTCAAGAAGCTAAAATCTCTTGATATATCTTACTGCTTCAGCATTAAGGAATTGCCTGAAGACTTTGGTGAATTAAGCAGCTTAAGAGAGCTTAACATGAGAGACTGCTCAAGATTGCAAGAGCTGCCTCTATCAGTTTCAAATCTGGAGCATTTAAAAGTTATAGGTGATGATGAGACACAAAGTTTATGGCAACCCTTCTTACCTATCAAAGGCACACACATAGTAGTGGTCAAAGAGAATATCAACCTAAATTGGCTCCCCAAGTTTCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

209

Amino Acids

23.54

Weight (kDa)

6.95

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 116 - 173 4.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 137, 282
AcuI CTGAAG 3 cut(s) 285, 373, 429
AfaI GTAC 1 cut(s) 316
AfiI CCNNNNNNNGG 1 cut(s) 563
AflII CTTAAG 1 cut(s) 433
AgsI TTSAA 2 cut(s) 55, 492
AluBI AGCT 8 cut(s) 101, 226, 250, 280, 361, 432, 442, 475
AluI AGCT 8 cut(s) 101, 226, 250, 280, 361, 432, 442, 475
Alw26I GTCTC 3 cut(s) 65, 447, 521
AoxI GGCC 2 cut(s) 137, 282
ApeKI GCWGC 2 cut(s) 429, 475
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 3 cut(s) 226, 431, 530
BalI TGGCCA 2 cut(s) 139, 284
BbsI GAAGAC 1 cut(s) 417
BbvI GCAGC 2 cut(s) 441, 462
BccI CCATC 1 cut(s) 34
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 3 cut(s) 65, 447, 521
BfaI CTAG 2 cut(s) 146, 218
BfrI CTTAAG 1 cut(s) 433
BisI GCNGC 2 cut(s) 430, 476
BlpI GCTNAGC 2 cut(s) 227, 322
BlsI GCNGC 2 cut(s) 431, 477
Bme1390I CCNGG 1 cut(s) 336
BmiI GGNNCC 2 cut(s) 350, 611
BmrFI CCNGG 1 cut(s) 336
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 24, 240
BmuI ACTGGG 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 417
BpmI CTGGAG 2 cut(s) 47, 518
Bpu10I CCTNAGC 1 cut(s) 305
Bpu1102I GCTNAGC 2 cut(s) 227, 322
BpuEI CTTGAG 2 cut(s) 338, 445
BpuMI CCSGG 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse1I ACTGG 2 cut(s) 201, 349
Bse3DI GCAATG 3 cut(s) 115, 178, 185
BseGI GGATG 1 cut(s) 26
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMI GCAATG 3 cut(s) 115, 178, 185
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 2 cut(s) 201, 349
BseXI GCAGC 2 cut(s) 441, 462
BshFI GGCC 2 cut(s) 139, 284
BsiSI CCGG 1 cut(s) 335
BslI CCNNNNNNNGG 1 cut(s) 563
BsmAI GTCTC 3 cut(s) 65, 447, 521
BsnI GGCC 2 cut(s) 139, 284
Bso31I GGTCTC 1 cut(s) 65
Bsp1407I TGTACA 1 cut(s) 314
Bsp1720I GCTNAGC 2 cut(s) 227, 322
BspANI GGCC 2 cut(s) 139, 284
BspCNI CTCAG 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 350, 611
BspTI CTTAAG 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 3 cut(s) 115, 178, 185
BsrGI TGTACA 1 cut(s) 314
BsrI ACTGG 2 cut(s) 201, 349
Bst4CI ACNGT 1 cut(s) 242
BstAFI CTTAAG 1 cut(s) 433
BstAPI GCANNNNNTGC 3 cut(s) 46, 191, 475
BstAUI TGTACA 1 cut(s) 314
BstC8I GCNNGC 2 cut(s) 192, 282
BstDEI CTNAG 3 cut(s) 227, 305, 322
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 1 cut(s) 26
BstMAI GTCTC 3 cut(s) 65, 447, 521
BstMWI GCNNNNNNNGC 3 cut(s) 46, 191, 475
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 441, 462
BstV2I GAAGAC 1 cut(s) 417
BsuRI GGCC 2 cut(s) 139, 284
BtsCI GGATG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 208
Cac8I GCNNGC 2 cut(s) 192, 282
Csp6I GTAC 1 cut(s) 315
CspCI CAANNNNNGTGG 2 cut(s) 129, 164
CviAII CATG 5 cut(s) 46, 82, 131, 135, 448
CviQI GTAC 1 cut(s) 315
DdeI CTNAG 3 cut(s) 227, 305, 322
DraI TTTAAA 1 cut(s) 507
EaeI YGGCCR 2 cut(s) 137, 282
Eco31I GGTCTC 1 cut(s) 65
Eco57I CTGAAG 3 cut(s) 285, 373, 429
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 136
FaeI CATG 5 cut(s) 49, 85, 134, 138, 451
FatI CATG 5 cut(s) 45, 81, 130, 134, 447
Fnu4HI GCNGC 2 cut(s) 430, 476
FokI GGATG 1 cut(s) 13
Fsp4HI GCNGC 2 cut(s) 430, 476
FspBI CTAG 2 cut(s) 146, 218
GluI GCNGC 2 cut(s) 430, 476
GsuI CTGGAG 2 cut(s) 47, 518
HaeIII GGCC 2 cut(s) 139, 284
HapII CCGG 1 cut(s) 335
Hin1II CATG 5 cut(s) 49, 85, 134, 138, 451
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 1 cut(s) 211
HpaII CCGG 1 cut(s) 335
HphI GGTGA 3 cut(s) 226, 431, 530
Hpy166II GTNNAC 1 cut(s) 157
Hpy188III TCNNGA 6 cut(s) 64, 218, 355, 371, 462, 497
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 559
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 4 cut(s) 49, 134, 171, 469
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 191, 475
HpyF3I CTNAG 3 cut(s) 227, 305, 322
Hsp92II CATG 5 cut(s) 49, 85, 134, 138, 451
LmnI GCTCC 2 cut(s) 499, 615
Lsp1109I GCAGC 2 cut(s) 441, 462
LweI GCATC 2 cut(s) 24, 240
MaeI CTAG 2 cut(s) 146, 218
MboII GAAGA 4 cut(s) 79, 206, 278, 422
MfeI CAATTG 1 cut(s) 50
MlsI TGGCCA 2 cut(s) 139, 284
MluCI AATT 9 cut(s) 21, 50, 90, 122, 287, 293, 401, 422, 604
MluNI TGGCCA 2 cut(s) 139, 284
MnlI CCTC 3 cut(s) 294, 362, 489
Mox20I TGGCCA 2 cut(s) 139, 284
Mph1103I ATGCAT 1 cut(s) 136
MscI TGGCCA 2 cut(s) 139, 284
MseI TTAA 5 cut(s) 396, 425, 434, 444, 506
MslI CAYNNNNRTG 2 cut(s) 133, 578
Msp20I TGGCCA 2 cut(s) 139, 284
MspCI CTTAAG 1 cut(s) 433
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 336
MunI CAATTG 1 cut(s) 50
MwoI GCNNNNNNNGC 3 cut(s) 46, 191, 475
NciI CCSGG 1 cut(s) 336
NlaIII CATG 5 cut(s) 49, 85, 134, 138, 451
NlaIV GGNNCC 2 cut(s) 350, 611
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 1 cut(s) 134
PfeI GAWTC 1 cut(s) 211
PkrI GCNGC 2 cut(s) 431, 477
PspN4I GGNNCC 2 cut(s) 350, 611
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
RseI CAYNNNNRTG 2 cut(s) 133, 578
SaqAI TTAA 5 cut(s) 396, 425, 434, 444, 506
SatI GCNGC 2 cut(s) 430, 476
ScrFI CCNGG 1 cut(s) 336
SfaNI GCATC 2 cut(s) 24, 240
SmiMI CAYNNNNRTG 2 cut(s) 133, 578
SmlI CTYRAG 3 cut(s) 353, 433, 460
SmoI CTYRAG 3 cut(s) 353, 433, 460
Sse9I AATT 9 cut(s) 21, 50, 90, 122, 287, 293, 401, 422, 604
SspMI CTAG 2 cut(s) 146, 218
StyD4I CCNGG 1 cut(s) 334
TaaI ACNGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 341
TasI AATT 9 cut(s) 21, 50, 90, 122, 287, 293, 401, 422, 604
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 5 cut(s) 396, 425, 434, 444, 506
Tru9I TTAA 5 cut(s) 396, 425, 434, 444, 506
TscAI CASTG 1 cut(s) 208
TseI GCWGC 2 cut(s) 429, 475
TspDTI ATGAA 1 cut(s) 70
TspRI CASTG 1 cut(s) 208
Vha464I CTTAAG 1 cut(s) 433
XagI CCTNNNNNAGG 1 cut(s) 561
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 1 cut(s) 134
XspI CTAG 2 cut(s) 146, 218
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.