Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000531

Physical Location & Seq

Genomic Coordinates Reverse (-)

29052363 .. 29061507

Gene Structure 9145 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1211600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGCAGCGAGGGAGTGAGAGAGAGAGAGAGAGAGGGAAATTAATCAGAAGTCAGATGCCAGCCCCCTCTAACGCCAAGAGCCTAAGCTCTATTTTCCGCGGCGCCATCAAATCCAAACCCCCCGCCATACCTCTACCCTCCCCCGCCGAGGACGCCACCCTCAAGCACTTCGTCTCCACCCTCGACCCCTCCCCTCCCTCCAACCCCATTTCTCAGCGCTTCACCGCCTCCAAAAAGCCACCCTCCGACCTCAACGCTCCTTCTGCGCTCGCCGCCTTGAATTTGGACTCGCCCTCCGCTCCCGAAGATTCGCCGAAAGAACTGCCTCGGGATATCTTTTCGATATTAGACGGCAGTACTTCTGCAAATTCTCTTGATATCCAGGGAACAGAAGGTGAGAAATTTAAGGAAGATACATTGCTCTTACCATTTTATCCTGAGGTATCTCATGGTGCAATATCATTACGTCGCAAAGAAGTCACTCGTGAAAGGAAGCAAAAGTGGACTTTCGGTAGCGGTCAGGTTAATCGTTTTGGTCGGTTAGTTGACATGTGTGCCAACAAGCTAGGGACACACACCACTCTTCAGGTTTTTGGTAAATTGGGACGAGAAACTGGTGTGAAAGAATATAATTCATTGATTAAAATTTGCATAGAGAGGGCTAGGAGTAGTACGGATGAAGATGTTGGAGTAGAACAGTTTCACATGGCTTATCGAATTTTTAAAGCAATGAAGGAACAGGGTTTTCCTCTGGAGGAAGAAACGTATGGCCAATTACTTGCATATCTAACTGACATGGGTATGATAAAGGAATTTCAATATTTCTGTGGAGTTATCAAAGCTGGAAACCCTAGTTCAGTTGGCAGATTAGGATACTATGAGATGCTGTTATGGATCAGTGTCGATGATGAGGAAAAAATTAAAGAGCTCTCTAATTACATTGTAAGTGATGATGCAGGAAAGGAGTCCAATTTACAAGAGAATTATCTTTTGGCACTTTGTGAAAGGGACCGGACAACAGAGATTTTGCAGCTTTTGGAAAGGATGGACATAACCAAAATCTCATCTCTGCCTTTTGTGGATAGTATCTTTAGATGTTTAGGAAGGCAACTACTGGATTCCTTCGCGGAGAAGTTTCTTCTGGAATTTAAAGCCTGTGACTCTGCTGCAGAGAAGATTACAAGCTTCATCTACAGTTATGTTGTTGGCATTCCAAATTTAGCGGTTGAGGATGTCATTTCAGAATTCAATAAATGGCACAAGAAATTGGAGGTGACACCTTCCTCTGCATCATATGAGAAGCTTATCATGTACTGTTGTGATTCACTTAAGGTGCATGTTGCTCTTGAACTGGTTAATGAGATGTGTGAACTGGGGTTGACCTTTTCAATTGAGGCAGTACACCCCATTTTACGTGCTAGTGACGAGAGCTGTGATTTTAATCTGGTACGCAAAATCTATTCATTGATTTGCCAGTACAAATTAGAGCCAAACTGTGAAACTTTCAGGTGTATGATAAGTTTGTGCGTGAAAATGAAGGATTATGAAGGTGCTTATAGCATGCTGAGAGACGTGGAGGAAATGAATTTGGCACCCACTACTAGCATGTACAATGCTATAATGGGTGGATTTTTCCGAGAGAAGAACATTTATGGTGGGTTGAGGGTTCTCCAACAAATGAAAGCAGCAAATGTCAAACCAGATTCCCAGACATTCAGCTATCTAATTACCAACTGTAACTCTGAGAAGGATATTAACAAGTATTATGAAGAAATGAAGCAGTCTGGAATTCAATTTACCAAGCAAATTTTCATGGCACTAATACATGCATACGCAGCTTGTGGGCAGTTTGAAAACGCCAAACAGGTACTCTTGGATAATGGGATTCCAGTAAAGAACTTGAATGAAATTAAAAGCGTGCTTATCCACACCCTCGCATCACGTGGGCAATTGTGTGATGCATTTAATATATATGAAGAAATGAAGCAAGCTGGATGCAGTGTAGAGCCGAAAGCTGTTATAAGTCTTATTGAGCACTCTCAATCTGATGAAGAATTCAGCAAATTATTCTCTCTACTTGATGAATTAGATGATCCAGACCACTGGCTTGAGGGTTGCATGAGAACTATCTTACATTGTGTTCGGAACAAGCATTTAAGATCTGCCGTCAATTTGATCAAGCAGTTCAGGGATAAAATCTGTACTGACGAATTGGCTCTAGACGTAATTTTTGATCAGGTATTTTCCTTAATTTCAGAATTAGAACCTACATATTTGCAGTTGGGATTGGACTTGCTTCATGCTATGAAGAATGAGCTTGGCATCACACCTTCACGAAAATGTCTTGATTTTCTTCTCCACGCTTGTGGTAATGCCAAAGATCTGAAAAATGCCCGACGAATTTGGAAAGAATACGAAGCTACTGGCCTGCCTTACAACGCTTTAAGTTTTTTAAGGATGTATCAAGCCCTTTTGGCTGGAGGGGACCGCAATGCTGCGAGAAATTTACATGCTAAAATGCCTAAAGATGATCCCCATGTTCGCTATATCATCCAAGCATGTAAAACCGCATACCCAAAACCGAAGAGTGGGAAGAAAAAGAAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3 PPR_long

Protein Analysis

870

Amino Acids

98.27

Weight (kDa)

6.43

Isoelectric Point (pI)

44.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	431 - 593	7e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	521 - 579	9.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	532 - 579	4.5e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000531)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04790 AT4G04790 AT4G04790 AT4G21880 AT4G21880
fragaria_vesca	FvH4_3g21820 FvH4_3g21820 FvH4_3g21820 FvH4_3g21820 FvH4_3g21820 FvH4_3g22610 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040
malus_domestica	MD03G1211600.v1.1
prunus_persica	Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1
pyrus_communis	pycom03g16030
rosa_chinensis	RchiOBHm_Chr4g0410111 RchiOBHm_Chr5g0037541 RchiOBHm_Chr5g0037561
rosa_laevigata	RLG00000008455 RLG00000008458 RLG00000008461 RLG00000008464 RLG00000008467 RLG00000008471 RLG00000008474 RLG00000008477 RLG00000008480 RLG00000008483 RLG00000008486 RLG00000008489 RLG00000033787
rosa_multiflora	Rmu_co8203932.1_g000001 Rmu_sc0000365.1_g000042 Rmu_sc0000365.1_g000043 Rmu_sc0002521.1_g000032
rosa_roxburghii	Rroxscaffold_1G00043480 Rroxscaffold_1G00043550 Rroxscaffold_5G00354910 Rroxscaffold_5G00354940
rosa_rugosa	Rorug05G0165400
rosa_samantha	Rh4AG159300 Rh4AG159500 Rh4CG170700 Rh4CG170900 Rh4DG154300 Rh5AG254800 Rh5BG256500 Rh5CG290100 Rh5DG264900
rosa_wichuraiana	Rw4G013230 Rw4G013270 Rw5G023590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2021
AccB1I	GGYRCC	2 cut(s)	101, 1591
AccBSI	CCGCTC	1 cut(s)	299
AccII	CGCG	2 cut(s)	99, 1127
AclWI	GGATC	3 cut(s)	902, 2087, 2525
AcoI	YGGCCR	1 cut(s)	769
AcuI	CTGAAG	1 cut(s)	569
AcvI	CACGTG	1 cut(s)	1943
AcyI	GRCGYC	2 cut(s)	102, 153
AdeI	CACNNNGTG	1 cut(s)	1001
AfaI	GTAC	9 cut(s)	358, 673, 1313, 1401, 1449, 1478, 1610, 1869, 2203
AfeI	AGCGCT	1 cut(s)	218
AfiI	CCNNNNNNNGG	2 cut(s)	148, 2588
AflII	CTTAAG	1 cut(s)	1328
AflIII	ACRYGT	1 cut(s)	549
AgsI	TTSAA	8 cut(s)	280, 818, 1249, 1349, 1389, 1793, 1853, 1903
AjiI	CACGTC	1 cut(s)	1573
AjnI	CCWGG	1 cut(s)	381
AjuI	GAANNNNNNNTTGG	4 cut(s)	974, 1006, 1793, 1825
AleI	CACNNNNGTG	1 cut(s)	2364
Alw21I	GWGCWC	2 cut(s)	930, 2037
Alw26I	GTCTC	2 cut(s)	178, 1563
AlwI	GGATC	3 cut(s)	902, 2087, 2525
Ama87I	CYCGRG	1 cut(s)	327
Aor51HI	AGCGCT	1 cut(s)	218
AoxI	GGCC	2 cut(s)	769, 2425
ApeKI	GCWGC	6 cut(s)	4, 1030, 1166, 1685, 1835, 2495
ArsI	GACNNNNNNTTYG	2 cut(s)	2213, 2245
AseI	ATTAAT	1 cut(s)	41
Asp700I	GAANNNNTTC	1 cut(s)	699
AspLEI	GCGC	3 cut(s)	104, 219, 268
AspS9I	GGNCC	2 cut(s)	1009, 2485
AsuHPI	GGTGA	3 cut(s)	214, 407, 1285
AvaI	CYCGRG	1 cut(s)	327
AvaII	GGWCC	2 cut(s)	1009, 2485
AxyI	CCTNAGG	1 cut(s)	438
BalI	TGGCCA	1 cut(s)	771
BanI	GGYRCC	2 cut(s)	101, 1591
BanII	GRGCYC	1 cut(s)	930
BauI	CACGAG	1 cut(s)	483
BbrPI	CACGTG	1 cut(s)	1943
Bbv12I	GWGCWC	2 cut(s)	930, 2037
BbvI	GCAGC	6 cut(s)	16, 1042, 1153, 1697, 1847, 2482
BccI	CCATC	2 cut(s)	113, 1039
BceAI	ACGGC	2 cut(s)	367, 2150
BcgI	CGANNNNNNTGC	2 cut(s)	304, 338
BciT130I	CCWGG	1 cut(s)	383
BciVI	GTATCC	1 cut(s)	866
BclI	TGATCA	2 cut(s)	2175, 2233
BcoDI	GTCTC	2 cut(s)	178, 1563
BfaI	CTAG	6 cut(s)	566, 663, 852, 1419, 1602, 2219
BfmI	CTRYAG	2 cut(s)	1167, 1192
BfoI	RGCGCY	2 cut(s)	105, 220
BfrI	CTTAAG	1 cut(s)	1328
BfuI	GTATCC	1 cut(s)	866
BglI	GCCNNNNNGGC	1 cut(s)	2474
BglII	AGATCT	2 cut(s)	2159, 2380
BisI	GCNGC	8 cut(s)	5, 100, 273, 1031, 1167, 1686, 1836, 2496
BlsI	GCNGC	8 cut(s)	6, 101, 274, 1032, 1168, 1687, 1837, 2497
BmcAI	AGTACT	1 cut(s)	358
Bme1390I	CCNGG	1 cut(s)	383
Bme18I	GGWCC	2 cut(s)	1009, 2485
BmeT110I	CYCGRG	1 cut(s)	327
BmgBI	CACGTC	1 cut(s)	1573
BmgT120I	GGNCC	2 cut(s)	1009, 2485
BmiI	GGNNCC	4 cut(s)	103, 1010, 1593, 2486
BmrFI	CCNGG	1 cut(s)	383
BmrI	ACTGGG	1 cut(s)	1382
BmsI	GCATC	8 cut(s)	45, 873, 943, 1298, 1946, 1948, 1985, 2331
BmuI	ACTGGG	1 cut(s)	1382
BpmI	CTGGAG	2 cut(s)	773, 2499
Bpu10I	CCTNAGC	1 cut(s)	83
BpuEI	CTTGAG	2 cut(s)	146, 2129
BsaAI	YACGTR	2 cut(s)	1415, 1943
BsaBI	GATNNNNATC	1 cut(s)	1440
BsaHI	GRCGYC	2 cut(s)	102, 153
BsaJI	CCNNGG	4 cut(s)	97, 147, 326, 382
BsaWI	WCCGGW	1 cut(s)	1011
BsaXI	ACNNNNNCTCC	4 cut(s)	158, 188, 278, 308
Bsc4I	CCNNNNNNNGG	2 cut(s)	148, 2588
Bse1I	ACTGG	8 cut(s)	619, 1119, 1356, 1377, 1474, 1889, 2108, 2428
Bse21I	CCTNAGG	1 cut(s)	438
Bse3DI	GCAATG	3 cut(s)	416, 735, 2497
Bse8I	GATNNNNATC	1 cut(s)	1440
BseBI	CCWGG	1 cut(s)	383
BseDI	CCNNGG	4 cut(s)	97, 147, 326, 382
BseGI	GGATG	6 cut(s)	682, 1050, 1237, 2000, 2463, 2550
BseJI	GATNNNNATC	1 cut(s)	1440
BseLI	CCNNNNNNNGG	2 cut(s)	148, 2588
BseMI	GCAATG	3 cut(s)	416, 735, 2497
BseMII	CTCAG	4 cut(s)	227, 429, 1556, 1734
BseNI	ACTGG	8 cut(s)	619, 1119, 1356, 1377, 1474, 1889, 2108, 2428
BseXI	GCAGC	6 cut(s)	16, 1042, 1153, 1697, 1847, 2482
Bsh1236I	CGCG	2 cut(s)	99, 1127
BshFI	GGCC	2 cut(s)	771, 2427
BshNI	GGYRCC	2 cut(s)	101, 1591
BsiHKAI	GWGCWC	2 cut(s)	930, 2037
BsiHKCI	CYCGRG	1 cut(s)	327
BsiSI	CCGG	1 cut(s)	1012
BslFI	GGGAC	4 cut(s)	583, 618, 1022, 2498
BslI	CCNNNNNNNGG	2 cut(s)	148, 2588
BsmAI	GTCTC	2 cut(s)	178, 1563
BsmBI	CGTCTC	2 cut(s)	178, 1563
BsmFI	GGGAC	4 cut(s)	583, 618, 1022, 2498
BsmI	GAATGC	1 cut(s)	1209
BsnI	GGCC	2 cut(s)	771, 2427
BsoBI	CYCGRG	1 cut(s)	327
Bsp1286I	GDGCHC	2 cut(s)	930, 2037
Bsp1407I	TGTACA	1 cut(s)	1608
Bsp143I	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
BspANI	GGCC	2 cut(s)	771, 2427
BspCNI	CTCAG	4 cut(s)	226, 430, 1557, 1735
BspFNI	CGCG	2 cut(s)	99, 1127
BspLI	GGNNCC	4 cut(s)	103, 1010, 1593, 2486
BspMAI	CTGCAG	1 cut(s)	1171
BspPI	GGATC	3 cut(s)	902, 2087, 2525
BspT107I	GGYRCC	2 cut(s)	101, 1591
BspTI	CTTAAG	1 cut(s)	1328
BsrBI	CCGCTC	1 cut(s)	299
BsrDI	GCAATG	3 cut(s)	416, 735, 2497
BsrGI	TGTACA	1 cut(s)	1608
BsrI	ACTGG	8 cut(s)	619, 1119, 1356, 1377, 1474, 1889, 2108, 2428
BssECI	CCNNGG	4 cut(s)	97, 147, 326, 382
BssMI	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
BssNI	GRCGYC	2 cut(s)	102, 153
BssSI	CACGAG	1 cut(s)	483
Bst2BI	CACGAG	1 cut(s)	483
Bst2UI	CCWGG	1 cut(s)	383
Bst4CI	ACNGT	5 cut(s)	699, 1196, 1316, 1496, 1736
Bst6I	CTCTTC	2 cut(s)	588, 2579
BstACI	GRCGYC	2 cut(s)	102, 153
BstAFI	CTTAAG	1 cut(s)	1328
BstAUI	TGTACA	1 cut(s)	1608
BstBAI	YACGTR	2 cut(s)	1415, 1943
BstC8I	GCNNGC	6 cut(s)	60, 270, 1562, 1919, 1989, 2429
BstDEI	CTNAG	5 cut(s)	83, 213, 438, 1565, 1743
BstDSI	CCRYGG	1 cut(s)	97
BstF5I	GGATG	6 cut(s)	682, 1050, 1237, 2000, 2463, 2550
BstFNI	CGCG	2 cut(s)	99, 1127
BstH2I	RGCGCY	2 cut(s)	105, 220
BstHHI	GCGC	3 cut(s)	104, 219, 268
BstKTI	GATC	7 cut(s)	897, 2095, 2162, 2178, 2236, 2383, 2533
BstMAI	GTCTC	2 cut(s)	178, 1563
BstMBI	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
BstMWI	GCNNNNNNNGC	5 cut(s)	152, 263, 272, 1835, 2474
BstNI	CCWGG	1 cut(s)	383
BstNSI	RCATGY	7 cut(s)	553, 1340, 1564, 1609, 1829, 2513, 2562
BstSCI	CCNGG	1 cut(s)	381
BstSFI	CTRYAG	2 cut(s)	1167, 1192
BstUI	CGCG	2 cut(s)	99, 1127
BstV1I	GCAGC	6 cut(s)	16, 1042, 1153, 1697, 1847, 2482
BstX2I	RGATCY	2 cut(s)	2159, 2380
BstXI	CCANNNNNNTGG	2 cut(s)	2103, 2366
BstYI	RGATCY	2 cut(s)	2159, 2380
Bsu36I	CCTNAGG	1 cut(s)	438
BsuI	GTATCC	1 cut(s)	866
BsuRI	GGCC	2 cut(s)	771, 2427
BtgI	CCRYGG	1 cut(s)	97
BtrI	CACGTC	1 cut(s)	1573
BtsCI	GGATG	6 cut(s)	682, 1050, 1237, 2000, 2463, 2550
BtsI	GCAGTG	1 cut(s)	2005
BtsIMutI	CAGTG	3 cut(s)	904, 2005, 2101
Cac8I	GCNNGC	6 cut(s)	60, 270, 1562, 1919, 1989, 2429
CfoI	GCGC	3 cut(s)	104, 219, 268
Cfr13I	GGNCC	2 cut(s)	1009, 2485
Cfr42I	CCGCGG	1 cut(s)	100
CseI	GACGC	1 cut(s)	161
Csp6I	GTAC	9 cut(s)	357, 672, 1312, 1400, 1448, 1477, 1609, 1868, 2202
CviQI	GTAC	9 cut(s)	357, 672, 1312, 1400, 1448, 1477, 1609, 1868, 2202
DdeI	CTNAG	5 cut(s)	83, 213, 438, 1565, 1743
DinI	GGCGCC	1 cut(s)	103
DpnI	GATC	7 cut(s)	896, 2094, 2161, 2177, 2235, 2382, 2532
DpnII	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
DraI	TTTAAA	2 cut(s)	724, 1150
DraIII	CACNNNGTG	1 cut(s)	1001
EaeI	YGGCCR	1 cut(s)	769
Eam1104I	CTCTTC	2 cut(s)	588, 2579
EarI	CTCTTC	2 cut(s)	588, 2579
Ecl136II	GAGCTC	1 cut(s)	928
Eco24I	GRGCYC	1 cut(s)	930
Eco32I	GATATC	2 cut(s)	334, 379
Eco47I	GGWCC	2 cut(s)	1009, 2485
Eco47III	AGCGCT	1 cut(s)	218
Eco53kI	GAGCTC	1 cut(s)	928
Eco57I	CTGAAG	1 cut(s)	569
Eco72I	CACGTG	1 cut(s)	1943
Eco81I	CCTNAGG	1 cut(s)	438
Eco88I	CYCGRG	1 cut(s)	327
EcoICRI	GAGCTC	1 cut(s)	928
EcoRI	GAATTC	3 cut(s)	1244, 1788, 2054
EcoRII	CCWGG	1 cut(s)	381
EcoRV	GATATC	2 cut(s)	334, 379
EcoT22I	ATGCAT	2 cut(s)	1831, 1963
EcoT38I	GRGCYC	1 cut(s)	930
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
Esp3I	CGTCTC	2 cut(s)	178, 1563
FaqI	GGGAC	4 cut(s)	583, 618, 1022, 2498
FauI	CCCGC	2 cut(s)	130, 151
FauNDI	CATATG	1 cut(s)	1294
FbaI	TGATCA	2 cut(s)	2175, 2233
Fnu4HI	GCNGC	8 cut(s)	5, 100, 273, 1031, 1167, 1686, 1836, 2496
FokI	GGATG	6 cut(s)	689, 1057, 1244, 2007, 2470, 2537
FriOI	GRGCYC	1 cut(s)	930
Fsp4HI	GCNGC	8 cut(s)	5, 100, 273, 1031, 1167, 1686, 1836, 2496
FspBI	CTAG	6 cut(s)	566, 663, 852, 1419, 1602, 2219
GlaI	GCGC	3 cut(s)	103, 218, 267
GluI	GCNGC	8 cut(s)	5, 100, 273, 1031, 1167, 1686, 1836, 2496
GsuI	CTGGAG	2 cut(s)	773, 2499
HaeII	RGCGCY	2 cut(s)	105, 220
HaeIII	GGCC	2 cut(s)	771, 2427
HapII	CCGG	1 cut(s)	1012
HgaI	GACGC	1 cut(s)	161
HhaI	GCGC	3 cut(s)	104, 219, 268
Hin1I	GRCGYC	2 cut(s)	102, 153
Hin6I	GCGC	3 cut(s)	102, 217, 266
HinP1I	GCGC	3 cut(s)	102, 217, 266
HincII	GTYRAC	2 cut(s)	547, 1380
HindII	GTYRAC	2 cut(s)	547, 1380
HindIII	AAGCTT	2 cut(s)	1183, 1301
HinfI	GANTC	8 cut(s)	287, 308, 965, 1118, 1160, 1322, 1703, 1885
HpaII	CCGG	1 cut(s)	1012
HphI	GGTGA	3 cut(s)	214, 407, 1285
Hpy166II	GTNNAC	5 cut(s)	504, 547, 1370, 1380, 1402
Hpy8I	GTNNAC	5 cut(s)	504, 547, 1370, 1380, 1402
Hpy99I	CGWCG	2 cut(s)	471, 2400
HpyCH4III	ACNGT	5 cut(s)	699, 1196, 1316, 1496, 1736
HpyCH4IV	ACGT	6 cut(s)	466, 764, 1414, 1572, 1942, 2223
HpyF10VI	GCNNNNNNNGC	5 cut(s)	152, 263, 272, 1835, 2474
HpyF3I	CTNAG	5 cut(s)	83, 213, 438, 1565, 1743
HpySE526I	ACGT	6 cut(s)	466, 764, 1414, 1572, 1942, 2223
Hsp92I	GRCGYC	2 cut(s)	102, 153
HspAI	GCGC	3 cut(s)	102, 217, 266
KasI	GGCGCC	1 cut(s)	101
Ksp22I	TGATCA	2 cut(s)	2175, 2233
KspI	CCGCGG	1 cut(s)	100
Kzo9I	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
LmnI	GCTCC	2 cut(s)	262, 304
Lsp1109I	GCAGC	6 cut(s)	16, 1042, 1153, 1697, 1847, 2482
LweI	GCATC	8 cut(s)	45, 873, 943, 1298, 1946, 1948, 1985, 2331
MaeI	CTAG	6 cut(s)	566, 663, 852, 1419, 1602, 2219
MaeII	ACGT	6 cut(s)	466, 764, 1414, 1572, 1942, 2223
MaeIII	GTNAC	5 cut(s)	478, 1157, 1273, 1421, 1736
MalI	GATC	7 cut(s)	896, 2094, 2161, 2177, 2235, 2382, 2532
MbiI	CCGCTC	1 cut(s)	299
MboI	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
MfeI	CAATTG	2 cut(s)	1389, 1949
MflI	RGATCY	2 cut(s)	2159, 2380
MhlI	GDGCHC	2 cut(s)	930, 2037
MlsI	TGGCCA	1 cut(s)	771
MluNI	TGGCCA	1 cut(s)	771
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	3 cut(s)	281, 974, 1154
MmeI	TCCRAC	4 cut(s)	225, 270, 667, 1696
Mox20I	TGGCCA	1 cut(s)	771
Mph1103I	ATGCAT	2 cut(s)	1831, 1963
MroXI	GAANNNNTTC	1 cut(s)	699
MscI	TGGCCA	1 cut(s)	771
MslI	CAYNNNNRTG	3 cut(s)	800, 2338, 2364
Msp20I	TGGCCA	1 cut(s)	771
MspA1I	CMGCKG	1 cut(s)	99
MspCI	CTTAAG	1 cut(s)	1328
MspI	CCGG	1 cut(s)	1012
MspR9I	CCNGG	1 cut(s)	383
MunI	CAATTG	2 cut(s)	1389, 1949
Mva1269I	GAATGC	1 cut(s)	1209
MvaI	CCWGG	1 cut(s)	383
MvnI	CGCG	2 cut(s)	99, 1127
MwoI	GCNNNNNNNGC	5 cut(s)	152, 263, 272, 1835, 2474
NarI	GGCGCC	1 cut(s)	102
NdeI	CATATG	1 cut(s)	1294
NdeII	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
NlaIV	GGNNCC	4 cut(s)	103, 1010, 1593, 2486
NmeAIII	GCCGAG	1 cut(s)	172
NmuCI	GTSAC	4 cut(s)	478, 1157, 1273, 1421
NsiI	ATGCAT	2 cut(s)	1831, 1963
NspI	RCATGY	7 cut(s)	553, 1340, 1564, 1609, 1829, 2513, 2562
OliI	CACNNNNGTG	1 cut(s)	2364
PaeI	GCATGC	1 cut(s)	1564
PciI	ACATGT	1 cut(s)	549
PctI	GAATGC	1 cut(s)	1209
PdmI	GAANNNNTTC	1 cut(s)	699
PfeI	GAWTC	5 cut(s)	308, 1118, 1322, 1703, 1885
PkrI	GCNGC	8 cut(s)	6, 101, 274, 1032, 1168, 1687, 1837, 2497
PleI	GAGTC	3 cut(s)	281, 973, 1154
PluTI	GGCGCC	1 cut(s)	105
PmaCI	CACGTG	1 cut(s)	1943
PmlI	CACGTG	1 cut(s)	1943
PpsI	GAGTC	3 cut(s)	281, 973, 1154
Ppu21I	YACGTR	2 cut(s)	1415, 1943
PscI	ACATGT	1 cut(s)	549
PshBI	ATTAAT	1 cut(s)	41
PsiI	TTATAA	1 cut(s)	2021
Psp124BI	GAGCTC	1 cut(s)	930
Psp6I	CCWGG	1 cut(s)	381
PspCI	CACGTG	1 cut(s)	1943
PspGI	CCWGG	1 cut(s)	381
PspN4I	GGNNCC	4 cut(s)	103, 1010, 1593, 2486
PspPI	GGNCC	2 cut(s)	1009, 2485
PstI	CTGCAG	1 cut(s)	1171
PsuI	RGATCY	2 cut(s)	2159, 2380
RsaI	GTAC	9 cut(s)	358, 673, 1313, 1401, 1449, 1478, 1610, 1869, 2203
RsaNI	GTAC	9 cut(s)	357, 672, 1312, 1400, 1448, 1477, 1609, 1868, 2202
RseI	CAYNNNNRTG	3 cut(s)	800, 2338, 2364
SacI	GAGCTC	1 cut(s)	930
SacII	CCGCGG	1 cut(s)	100
SatI	GCNGC	8 cut(s)	5, 100, 273, 1031, 1167, 1686, 1836, 2496
Sau3AI	GATC	7 cut(s)	894, 2092, 2159, 2175, 2233, 2380, 2530
Sau96I	GGNCC	2 cut(s)	1009, 2485
ScaI	AGTACT	1 cut(s)	358
SchI	GAGTC	3 cut(s)	281, 974, 1154
ScrFI	CCNGG	1 cut(s)	383
SduI	GDGCHC	2 cut(s)	930, 2037
SfaNI	GCATC	8 cut(s)	45, 873, 943, 1298, 1946, 1948, 1985, 2331
SfcI	CTRYAG	2 cut(s)	1167, 1192
SfoI	GGCGCC	1 cut(s)	103
Sfr303I	CCGCGG	1 cut(s)	100
SgrBI	CCGCGG	1 cut(s)	100
SinI	GGWCC	2 cut(s)	1009, 2485
SmiMI	CAYNNNNRTG	3 cut(s)	800, 2338, 2364
SmlI	CTYRAG	3 cut(s)	161, 1328, 2108
SmoI	CTYRAG	3 cut(s)	161, 1328, 2108
SphI	GCATGC	1 cut(s)	1564
SspDI	GGCGCC	1 cut(s)	101
SspI	AATATT	1 cut(s)	821
SspMI	CTAG	6 cut(s)	566, 663, 852, 1419, 1602, 2219
SstI	GAGCTC	1 cut(s)	930
StyD4I	CCNGG	1 cut(s)	381
TaaI	ACNGT	5 cut(s)	699, 1196, 1316, 1496, 1736
TaiI	ACGT	6 cut(s)	469, 767, 1417, 1575, 1945, 2226
TaqI	TCGA	4 cut(s)	183, 341, 715, 903
TatI	WGTACW	6 cut(s)	356, 1311, 1399, 1476, 1608, 2201
TauI	GCSGC	2 cut(s)	102, 275
TfiI	GAWTC	5 cut(s)	308, 1118, 1322, 1703, 1885
TscAI	CASTG	3 cut(s)	904, 2005, 2108
TseFI	GTSAC	4 cut(s)	478, 1157, 1273, 1421
TseI	GCWGC	6 cut(s)	4, 1030, 1166, 1685, 1835, 2495
Tsp45I	GTSAC	4 cut(s)	478, 1157, 1273, 1421
TspGWI	ACGGA	1 cut(s)	689
TspRI	CASTG	3 cut(s)	904, 2005, 2108
Vha464I	CTTAAG	1 cut(s)	1328
VpaK11BI	GGWCC	2 cut(s)	1009, 2485
VspI	ATTAAT	1 cut(s)	41
XbaI	TCTAGA	1 cut(s)	2218
XceI	RCATGY	7 cut(s)	553, 1340, 1564, 1609, 1829, 2513, 2562
XmnI	GAANNNNTTC	1 cut(s)	699
XspI	CTAG	6 cut(s)	566, 663, 852, 1419, 1602, 2219
ZrmI	AGTACT	1 cut(s)	358
Zsp2I	ATGCAT	2 cut(s)	1831, 1963

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.