Rw5G023590

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000531
Physical Location & Seq
Forward (+)
33320524 .. 33330980
10457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023590.1

Sequence Viewer

Length: 2451 bp
ATGAAAGCCAAAAAGCTAAGCTCTCTCTTTCGCTCTGCCATCAAATACAAACCCTCCTCCTCCTCTTCCTCCGCCAAATCGCTACCCTCACCCGCCGAGGACGCCACTCTCAAATTCGTCTCTGCTTCCGGTAATCGTCAATCTAACTCTTCCTTTGTTTCCGTCACTGTTTGTAACCAAAATGGAGAATTAGAAACCCTAAATACTGAAGATTCGACTAGGCAGCTCCCTGGGGACATTTATTCAATACTGTGCGGTGGTACTTATCCTGATATCCAGGAAATCGATGATGCAGAATCTTTGGAGAGGGAATTGGACTTACCATGGTTTCCAGATTTATCCCATAGTGCGATGCCATTGCGCCGCAAAGAAGTTAGTCGCGAAAGGAAGCAGAGGTTTGCTTTCGGCAACACTCAGGGTATCCGTTTTGATAAGTTAGTCGACATGTGTGCCAATAGGCTAGGGACTGAAACCACTATTCAAGTGTTTGACAAATTGGGAAGAGAAACGGGTGTGAAAGAATGTAATGCATTGATAAAGAGATGCATAGAGGGAGCTAGGATTGCTCCTGATGAAGTAGTTGGAGAAAAGCATATGCAGTTGGCTTTTGAAATTTTTAAGTCAATGAAGGAACAGGGTTTCCCGCTTGAAGAGGAAACATATGGTCCGTTTCTTGAATATCTTAATGACATGGGTATGACAAAAGAGTTTTTCTTTTTCTGCCGAGTTATCAAAGATGACAACCCTTTGTCAGTTTCAAGATTAGGATACTATGAGATGATGTTGTGGATCAGAGTCAATGATGAAAAAAAGATTCAAGAGCTCTGTAATGACATTGAGAGTTACGATGGAGTAAACAAGTCAATTTTACAAGAGAATTATTTGTTAGCACTTTGTGAAAGTGACCGGACGAAGGAGATTTTGAAGCTGTTAGAAAGTATGGACATCACAAAGGTCTCATCACCGGATTTTGTGGCACGTATCTTTAAATGCCTGGGAAGGCTCCTATTGGAGTCTTTTGCAGAGAAGGTCCTTCTGGAATTTAAAGCCAGTGACTGTGCAGCAGGGAATACTTCAAACTTGATCTATAGTTATGTTGTTGGCATTCCGAATTTAGCGGTTGAGGATGTCATTTCAAAATTTAAAAACTTGCACACAAAACTTGAGGTGACACCTTCATCTGTGTCATATGAGAAGCTTATCATCTACTGTTGCGGTTCACTTAAGGTGCATGTGGCTCTTGAGCTGGTGGATGAGATGTCTGAACAGGGTTTGACATTTTCGATGGAGGCATTACACTCCATTTTACAAGCTAGTAGTGAGAGTTGTGACTTTAATCTGGTTCACCAAATTTATTCAGTGATTAGCCGAGGCAATTTGAAGCCCAATAGCGAAACTTTCAGGAGTATGATAAATCTTTGTGTGAAAATGAAGGATTATGAGGGTGCCTATAGCATGCTGAGTGATTTGGAGAAAATGAATTTGACGCCCACAGCTGCCATGTACAATGCTATAATGGCTGGATATTTCCGAGAGAAGAACTTGTCCGGTGGGTTAAAGGTTCTCAAACAAATGAAAGAAGCAGATGTAAAACCAGATTCCCACACTTTCAGCCATCTAATAACCAACTGCAATTCTGAAGAGGACATAAACATGTATTATGAAGAAATGAAGCGATCTGGAATTCAAGTTACCAAGCAAGTTTTCATGGCACTTGTAAATGCATATGCAGCTTGTGGACAGTTTGAAAAGGCAAAACAGGTACTCTTAGACAAGGGAATTCCAGTAAAGAGCTTCAATGAGATTAAAAGTGTGCTTGTCCAAGCCCTTGCATCACATGGGCAATTGTCTGATGCATTTAATACATATGAAGAGATCAAGCAAGCTGGATGCAGTTTGGAGCCGAAAGCTGTTATAAGTCTTATCGAGCACCTTCAATCTGATGAAGAAGCATTAAGCAGATTACTCCTCCTACTTGAGACACTAAATGATCCAAACTATTGGCTTGATGGTTGCTTGAGAACTATCTTATATTGTGTTCGTTACAAGCATTTAAGACCTGCCGTCAATTTGCTTGAGCAACTCAGGGATCAGGTCTGTACTGACGAATTAGCTCTAGAAGTAATTTTTGATAAGCTTGGCCTCACCCCTTCACGAAAATGTCTCGATTTTCTTCTCCACGCTTGTGTCACTGCCAAAGATCTGCAAAGTTCCCTATTTGTATGGCAAGAATATCAAGCCGCTGGCTTGCCTTACAATACCTTAAGCTTTTTAAGGATGTATCAAGCCCTTTTGGCTGCAGGGGACCGAAAGGCTGCAAAAGTTTTGGGACGTAAAATTCCTAAAGATGATCCTCATGTTCGGTCTATCATTGAATCATGTACACTGACTTATTTAGAGAAGAAGGAAGAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAATGA

Protein Analysis

816

Amino Acids

92.3

Weight (kDa)

6.32

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 398 - 561 5.1e-13 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 471 - 586 2.5e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 486 - 544 2.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 497 - 544 2.2e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000531)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04790 AT4G04790 AT4G04790 AT4G21880 AT4G21880
fragaria_vesca FvH4_3g21820 FvH4_3g21820 FvH4_3g21820 FvH4_3g21820 FvH4_3g21820 FvH4_3g22610 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040 FvH4_7g08040
malus_domestica MD03G1211600.v1.1
prunus_persica Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1 Prupe.4G202300_v2.0.a1
pyrus_communis pycom03g16030
rosa_chinensis RchiOBHm_Chr4g0410111 RchiOBHm_Chr5g0037541 RchiOBHm_Chr5g0037561
rosa_laevigata RLG00000008455 RLG00000008458 RLG00000008461 RLG00000008464 RLG00000008467 RLG00000008471 RLG00000008474 RLG00000008477 RLG00000008480 RLG00000008483 RLG00000008486 RLG00000008489 RLG00000033787
rosa_multiflora Rmu_co8203932.1_g000001 Rmu_sc0000365.1_g000042 Rmu_sc0000365.1_g000043 Rmu_sc0002521.1_g000032
rosa_roxburghii Rroxscaffold_1G00043480 Rroxscaffold_1G00043550 Rroxscaffold_5G00354910 Rroxscaffold_5G00354940
rosa_rugosa Rorug05G0165400
rosa_samantha Rh4AG159300 Rh4AG159500 Rh4CG170700 Rh4CG170900 Rh4DG154300 Rh5AG254800 Rh5BG256500 Rh5CG290100 Rh5DG264900
rosa_wichuraiana Rw4G013230 Rw4G013270 Rw5G023590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1916
Acc36I ACCTGC 1 cut(s) 2068
AccB1I GGYRCC 1 cut(s) 1445
AccI GTMKAC 1 cut(s) 441
AccII CGCG 1 cut(s) 381
AciI CCGC 8 cut(s) 72, 93, 255, 364, 644, 1118, 1215, 2241
AclWI GGATC 4 cut(s) 797, 1985, 2097, 2345
AcuI CTGAAG 2 cut(s) 228, 1659
AcyI GRCGYC 2 cut(s) 102, 1487
AdeI CACNNNGTG 1 cut(s) 896
AfaI GTAC 5 cut(s) 262, 1505, 1764, 2101, 2383
AfiI CCNNNNNNNGG 1 cut(s) 913
AflII CTTAAG 2 cut(s) 1223, 2263
AflIII ACRYGT 2 cut(s) 444, 1653
AhdI GACNNNNNGTC 1 cut(s) 2063
AjnI CCWGG 3 cut(s) 229, 276, 993
AjuI GAANNNNNNNTTGG 2 cut(s) 1688, 1720
AleI CACNNNNGTG 1 cut(s) 2184
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
Alw21I GWGCWC 2 cut(s) 825, 1932
Alw26I GTCTC 4 cut(s) 124, 961, 1973, 2168
AlwI GGATC 4 cut(s) 797, 1985, 2097, 2345
AlwNI CAGNNNCTG 1 cut(s) 1056
AoxI GGCC 1 cut(s) 2140
ApeKI GCWGC 6 cut(s) 223, 1061, 1496, 1730, 2297, 2315
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 3 cut(s) 665, 1030, 2305
AsuHPI GGTGA 5 cut(s) 81, 954, 1180, 1337, 2137
AvaII GGWCC 3 cut(s) 665, 1030, 2305
BanI GGYRCC 1 cut(s) 1445
BanII GRGCYC 1 cut(s) 825
Bbv12I GWGCWC 2 cut(s) 825, 1932
BbvI GCAGC 6 cut(s) 235, 1073, 1483, 1742, 2284, 2302
BccI CCATC 5 cut(s) 47, 842, 1279, 1623, 2003
BceAI ACGGC 1 cut(s) 2048
BcgI CGANNNNNNTGC 4 cut(s) 340, 374, 431, 465
BciT130I CCWGG 3 cut(s) 231, 278, 995
BciVI GTATCC 2 cut(s) 431, 761
BcoDI GTCTC 4 cut(s) 124, 961, 1973, 2168
BfaI CTAG 5 cut(s) 219, 461, 558, 1314, 2117
BfmI CTRYAG 3 cut(s) 1087, 1450, 2298
BfrI CTTAAG 2 cut(s) 1223, 2263
BfuAI ACCTGC 1 cut(s) 2068
BfuI GTATCC 2 cut(s) 431, 761
BglI GCCNNNNNGGC 1 cut(s) 2294
BglII AGATCT 1 cut(s) 2200
BisI GCNGC 8 cut(s) 224, 364, 1062, 1497, 1731, 2241, 2298, 2316
BlpI GCTNAGC 1 cut(s) 17
BlsI GCNGC 8 cut(s) 225, 365, 1063, 1498, 1732, 2242, 2299, 2317
Bme1390I CCNGG 3 cut(s) 231, 278, 995
Bme18I GGWCC 3 cut(s) 665, 1030, 2305
BmeRI GACNNNNNGTC 1 cut(s) 2063
BmgT120I GGNCC 3 cut(s) 665, 1030, 2305
BmiI GGNNCC 4 cut(s) 1004, 1447, 1902, 2306
BmrFI CCNGG 3 cut(s) 231, 278, 995
BmsI GCATC 6 cut(s) 280, 342, 533, 1841, 1843, 1880
Bpu1102I GCTNAGC 1 cut(s) 17
BpuEI CTTGAG 5 cut(s) 1184, 1262, 1997, 2038, 2096
Bsa29I ATCGAT 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 980
BsaHI GRCGYC 2 cut(s) 102, 1487
BsaI GGTCTC 1 cut(s) 961
BsaJI CCNNGG 6 cut(s) 96, 229, 230, 323, 994, 1369
BsaWI WCCGGW 4 cut(s) 128, 906, 964, 1547
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 913
Bse1I ACTGG 2 cut(s) 1050, 1784
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 3 cut(s) 231, 278, 995
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 6 cut(s) 96, 229, 230, 323, 994, 1369
BseGI GGATG 4 cut(s) 1132, 1258, 1895, 2283
BseLI CCNNNNNNNGG 1 cut(s) 913
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 3 cut(s) 428, 1451, 2098
BseNI ACTGG 2 cut(s) 1050, 1784
BseRI GAGGAG 4 cut(s) 46, 49, 52, 1958
BseXI GCAGC 6 cut(s) 235, 1073, 1483, 1742, 2284, 2302
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 2142
BshNI GGYRCC 1 cut(s) 1445
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 825, 1932
BsiSI CCGG 4 cut(s) 129, 907, 965, 1548
BslFI GGGAC 4 cut(s) 248, 478, 2318, 2343
BslI CCNNNNNNNGG 1 cut(s) 913
BsmAI GTCTC 4 cut(s) 124, 961, 1973, 2168
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 4 cut(s) 248, 478, 2318, 2343
BsmI GAATGC 1 cut(s) 1104
BsnI GGCC 1 cut(s) 2142
Bso31I GGTCTC 1 cut(s) 961
Bsp1286I GDGCHC 2 cut(s) 825, 1932
Bsp1407I TGTACA 2 cut(s) 1503, 2381
Bsp143I GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
Bsp1720I GCTNAGC 1 cut(s) 17
Bsp19I CCATGG 1 cut(s) 323
Bsp68I TCGCGA 1 cut(s) 381
BspACI CCGC 8 cut(s) 72, 93, 255, 364, 644, 1118, 1215, 2241
BspANI GGCC 1 cut(s) 2142
BspCNI CTCAG 3 cut(s) 427, 1452, 2097
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 4 cut(s) 1004, 1447, 1902, 2306
BspMAI CTGCAG 1 cut(s) 2302
BspMI ACCTGC 1 cut(s) 2068
BspPI GGATC 4 cut(s) 797, 1985, 2097, 2345
BspT107I GGYRCC 1 cut(s) 1445
BspTI CTTAAG 2 cut(s) 1223, 2263
BspTNI GGTCTC 1 cut(s) 961
BsrDI GCAATG 1 cut(s) 356
BsrGI TGTACA 2 cut(s) 1503, 2381
BsrI ACTGG 2 cut(s) 1050, 1784
BssECI CCNNGG 6 cut(s) 96, 229, 230, 323, 994, 1369
BssMI GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
BssNI GRCGYC 2 cut(s) 102, 1487
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 3 cut(s) 231, 278, 995
Bst4CI ACNGT 5 cut(s) 169, 252, 1058, 1211, 1743
Bst6I CTCTTC 6 cut(s) 70, 154, 496, 645, 1635, 1866
BstACI GRCGYC 2 cut(s) 102, 1487
BstAFI CTTAAG 2 cut(s) 1223, 2263
BstAUI TGTACA 2 cut(s) 1503, 2381
BstBAI YACGTR 1 cut(s) 980
BstC8I GCNNGC 4 cut(s) 1457, 1884, 2245, 2249
BstDEI CTNAG 5 cut(s) 17, 414, 1460, 1768, 2084
BstDSI CCRYGG 1 cut(s) 323
BstF5I GGATG 4 cut(s) 1132, 1258, 1895, 2283
BstFNI CGCG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 8 cut(s) 792, 1086, 1679, 1878, 1993, 2092, 2203, 2353
BstMAI GTCTC 4 cut(s) 124, 961, 1973, 2168
BstMBI GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
BstMWI GCNNNNNNNGC 5 cut(s) 101, 563, 1517, 1730, 2294
BstNI CCWGG 3 cut(s) 231, 278, 995
BstNSI RCATGY 4 cut(s) 448, 1235, 1459, 1657
BstSCI CCNGG 3 cut(s) 229, 276, 993
BstSFI CTRYAG 3 cut(s) 1087, 1450, 2298
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 6 cut(s) 235, 1073, 1483, 1742, 2284, 2302
BstX2I RGATCY 1 cut(s) 2200
BstXI CCANNNNNNTGG 1 cut(s) 2001
BstYI RGATCY 1 cut(s) 2200
Bsu15I ATCGAT 1 cut(s) 285
BsuI GTATCC 2 cut(s) 431, 761
BsuRI GGCC 1 cut(s) 2142
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 323
BtgZI GCGATG 1 cut(s) 365
BtsCI GGATG 4 cut(s) 1132, 1258, 1895, 2283
BtsI GCAGTG 1 cut(s) 2190
BtsIMutI CAGTG 5 cut(s) 165, 1057, 1365, 2190, 2384
BtuMI TCGCGA 1 cut(s) 381
BveI ACCTGC 1 cut(s) 2068
Cac8I GCNNGC 4 cut(s) 1457, 1884, 2245, 2249
CaiI CAGNNNCTG 1 cut(s) 1056
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 3 cut(s) 665, 1030, 2305
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 2 cut(s) 110, 1495
Csp6I GTAC 5 cut(s) 261, 1504, 1763, 2100, 2382
CviQI GTAC 5 cut(s) 261, 1504, 1763, 2100, 2382
DdeI CTNAG 5 cut(s) 17, 414, 1460, 1768, 2084
DpnI GATC 8 cut(s) 791, 1085, 1678, 1877, 1992, 2091, 2202, 2352
DpnII GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
DraI TTTAAA 3 cut(s) 988, 1045, 1144
DraIII CACNNNGTG 1 cut(s) 896
DriI GACNNNNNGTC 1 cut(s) 2063
Eam1104I CTCTTC 6 cut(s) 70, 154, 496, 645, 1635, 1866
Eam1105I GACNNNNNGTC 1 cut(s) 2063
EarI CTCTTC 6 cut(s) 70, 154, 496, 645, 1635, 1866
EciI GGCGGA 1 cut(s) 61
Ecl136II GAGCTC 1 cut(s) 823
Eco130I CCWWGG 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 825
Eco31I GGTCTC 1 cut(s) 961
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 3 cut(s) 665, 1030, 2305
Eco53kI GAGCTC 1 cut(s) 823
Eco57I CTGAAG 2 cut(s) 228, 1659
EcoICRI GAGCTC 1 cut(s) 823
EcoO109I RGGNCCY 1 cut(s) 1030
EcoRI GAATTC 2 cut(s) 1683, 1779
EcoRII CCWGG 3 cut(s) 229, 276, 993
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 323
EcoT22I ATGCAT 4 cut(s) 532, 548, 1726, 1858
EcoT38I GRGCYC 1 cut(s) 825
ErhI CCWWGG 1 cut(s) 323
Esp3I CGTCTC 1 cut(s) 124
FaqI GGGAC 4 cut(s) 248, 478, 2318, 2343
FauI CCCGC 2 cut(s) 100, 651
FauNDI CATATG 5 cut(s) 594, 661, 1189, 1726, 1867
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 8 cut(s) 224, 364, 1062, 1497, 1731, 2241, 2298, 2316
FokI GGATG 4 cut(s) 1139, 1265, 1902, 2290
FriOI GRGCYC 1 cut(s) 825
Fsp4HI GCNGC 8 cut(s) 224, 364, 1062, 1497, 1731, 2241, 2298, 2316
FspBI CTAG 5 cut(s) 219, 461, 558, 1314, 2117
GlaI GCGC 1 cut(s) 362
GluI GCNGC 8 cut(s) 224, 364, 1062, 1497, 1731, 2241, 2298, 2316
HaeIII GGCC 1 cut(s) 2142
HapII CCGG 4 cut(s) 129, 907, 965, 1548
HgaI GACGC 2 cut(s) 110, 1495
HhaI GCGC 1 cut(s) 363
Hin1I GRCGYC 2 cut(s) 102, 1487
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 3 cut(s) 1196, 2135, 2266
HinfI GANTC 7 cut(s) 212, 296, 795, 814, 1013, 1598, 2375
HpaII CCGG 4 cut(s) 129, 907, 965, 1548
HphI GGTGA 5 cut(s) 81, 954, 1180, 1337, 2137
Hpy166II GTNNAC 6 cut(s) 442, 856, 1220, 1345, 1739, 2384
Hpy188I TCNGA 7 cut(s) 794, 1110, 1264, 1532, 1639, 1852, 1942
Hpy8I GTNNAC 6 cut(s) 442, 856, 1220, 1345, 1739, 2384
HpyCH4III ACNGT 5 cut(s) 169, 252, 1058, 1211, 1743
HpyCH4IV ACGT 2 cut(s) 979, 2332
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 563, 1517, 1730, 2294
HpyF3I CTNAG 5 cut(s) 17, 414, 1460, 1768, 2084
HpySE526I ACGT 2 cut(s) 979, 2332
Hsp92I GRCGYC 2 cut(s) 102, 1487
HspAI GCGC 1 cut(s) 361
Kzo9I GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
LmnI GCTCC 5 cut(s) 231, 554, 571, 1008, 1900
Lsp1109I GCAGC 6 cut(s) 235, 1073, 1483, 1742, 2284, 2302
LweI GCATC 6 cut(s) 280, 342, 533, 1841, 1843, 1880
MaeI CTAG 5 cut(s) 219, 461, 558, 1314, 2117
MaeII ACGT 2 cut(s) 979, 2332
MalI GATC 8 cut(s) 791, 1085, 1678, 1877, 1992, 2091, 2202, 2352
MboI GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
MfeI CAATTG 1 cut(s) 1844
MflI RGATCY 1 cut(s) 2200
MhlI GDGCHC 2 cut(s) 825, 1932
MlyI GAGTC 2 cut(s) 804, 1022
MmeI TCCRAC 1 cut(s) 562
Mph1103I ATGCAT 4 cut(s) 532, 548, 1726, 1858
MslI CAYNNNNRTG 4 cut(s) 695, 1652, 2158, 2184
MspA1I CMGCKG 2 cut(s) 1496, 2243
MspCI CTTAAG 2 cut(s) 1223, 2263
MspI CCGG 4 cut(s) 129, 907, 965, 1548
MspR9I CCNGG 3 cut(s) 231, 278, 995
MunI CAATTG 1 cut(s) 1844
Mva1269I GAATGC 1 cut(s) 1104
MvaI CCWGG 3 cut(s) 231, 278, 995
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 5 cut(s) 101, 563, 1517, 1730, 2294
NcoI CCATGG 1 cut(s) 323
NdeI CATATG 5 cut(s) 594, 661, 1189, 1726, 1867
NdeII GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
NlaIV GGNNCC 4 cut(s) 1004, 1447, 1902, 2306
NmeAIII GCCGAG 3 cut(s) 121, 749, 1394
NmuCI GTSAC 6 cut(s) 163, 902, 1052, 1168, 1328, 2188
NruI TCGCGA 1 cut(s) 381
NsiI ATGCAT 4 cut(s) 532, 548, 1726, 1858
NspI RCATGY 4 cut(s) 448, 1235, 1459, 1657
OliI CACNNNNGTG 1 cut(s) 2184
PaeI GCATGC 1 cut(s) 1459
PasI CCCWGGG 1 cut(s) 230
PciI ACATGT 2 cut(s) 444, 1653
PctI GAATGC 1 cut(s) 1104
PfeI GAWTC 5 cut(s) 212, 296, 814, 1598, 2375
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 8 cut(s) 225, 365, 1063, 1498, 1732, 2242, 2299, 2317
PleI GAGTC 2 cut(s) 803, 1021
PpsI GAGTC 2 cut(s) 803, 1021
Ppu21I YACGTR 1 cut(s) 980
PpuMI RGGWCCY 1 cut(s) 1030
PscI ACATGT 2 cut(s) 444, 1653
PsiI TTATAA 1 cut(s) 1916
Psp124BI GAGCTC 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 1030
Psp6I CCWGG 3 cut(s) 229, 276, 993
PspGI CCWGG 3 cut(s) 229, 276, 993
PspN4I GGNNCC 4 cut(s) 1004, 1447, 1902, 2306
PspPI GGNCC 3 cut(s) 665, 1030, 2305
PspPPI RGGWCCY 1 cut(s) 1030
PstI CTGCAG 1 cut(s) 2302
PstNI CAGNNNCTG 1 cut(s) 1056
PsuI RGATCY 1 cut(s) 2200
PvuII CAGCTG 1 cut(s) 1496
RruI TCGCGA 1 cut(s) 381
RsaI GTAC 5 cut(s) 262, 1505, 1764, 2101, 2383
RsaNI GTAC 5 cut(s) 261, 1504, 1763, 2100, 2382
RseI CAYNNNNRTG 4 cut(s) 695, 1652, 2158, 2184
SacI GAGCTC 1 cut(s) 825
SalI GTCGAC 1 cut(s) 440
SatI GCNGC 8 cut(s) 224, 364, 1062, 1497, 1731, 2241, 2298, 2316
Sau3AI GATC 8 cut(s) 789, 1083, 1676, 1875, 1990, 2089, 2200, 2350
Sau96I GGNCC 3 cut(s) 665, 1030, 2305
SchI GAGTC 2 cut(s) 804, 1022
ScrFI CCNGG 3 cut(s) 231, 278, 995
SduI GDGCHC 2 cut(s) 825, 1932
SfaNI GCATC 6 cut(s) 280, 342, 533, 1841, 1843, 1880
SfcI CTRYAG 3 cut(s) 1087, 1450, 2298
SinI GGWCC 3 cut(s) 665, 1030, 2305
SmiMI CAYNNNNRTG 4 cut(s) 695, 1652, 2158, 2184
SmlI CTYRAG 7 cut(s) 1163, 1223, 1241, 1976, 2017, 2075, 2263
SmoI CTYRAG 7 cut(s) 1163, 1223, 1241, 1976, 2017, 2075, 2263
SphI GCATGC 1 cut(s) 1459
SsiI CCGC 8 cut(s) 72, 93, 255, 364, 644, 1118, 1215, 2241
SspMI CTAG 5 cut(s) 219, 461, 558, 1314, 2117
SstI GAGCTC 1 cut(s) 825
StyD4I CCNGG 3 cut(s) 229, 276, 993
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 5 cut(s) 169, 252, 1058, 1211, 1743
TaiI ACGT 2 cut(s) 982, 2335
TaqI TCGA 6 cut(s) 215, 285, 441, 1283, 1926, 2166
TaqII GACCGA 2 cut(s) 2322, 2352
TatI WGTACW 3 cut(s) 1503, 2099, 2381
TauI GCSGC 2 cut(s) 366, 2243
TfiI GAWTC 5 cut(s) 212, 296, 814, 1598, 2375
TscAI CASTG 5 cut(s) 172, 1057, 1365, 2197, 2391
TseFI GTSAC 6 cut(s) 163, 902, 1052, 1168, 1328, 2188
TseI GCWGC 6 cut(s) 223, 1061, 1496, 1730, 2297, 2315
Tsp45I GTSAC 6 cut(s) 163, 902, 1052, 1168, 1328, 2188
TspGWI ACGGA 3 cut(s) 151, 413, 657
TspRI CASTG 5 cut(s) 172, 1057, 1365, 2197, 2391
Vha464I CTTAAG 2 cut(s) 1223, 2263
VpaK11BI GGWCC 3 cut(s) 665, 1030, 2305
XbaI TCTAGA 1 cut(s) 2116
XceI RCATGY 4 cut(s) 448, 1235, 1459, 1657
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 5 cut(s) 219, 461, 558, 1314, 2117
Zsp2I ATGCAT 4 cut(s) 532, 548, 1726, 1858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.