MD03G1267500.v1.1

Phenazine biosynthesis-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001362
Physical Location & Seq
Reverse (-)
35249641 .. 35252771
3131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1267500.v1.1.491

Sequence Viewer

Length: 936 bp
ATGTTGGGATCCCCTTTTTGGGAGCTCACATTGTGCCTTAGCATCATACTGGTGCAAAAAACTCGGAAAATCCGAGTTGGATACATGGTGGACGCGTTCACAGACTCGGCTTTCAAGGGGAACCCAGCAGCGGTTTGCTTGCTGGAGGAAGATAGAGACGACCAGTGGCTACAAGCTGTGGCCTTCGAGTTCAATCTCTCCCAGACGTGCTACTTGACTCGAATCAATGACTCTCACTCAGCTCCTCGGTTCGGTCTCAGATGGTTCTCGCCTACTAATGAGGTTGAGCTTTGCGGTCATGGAACATTAGCTTCTTCACACACTCTCTTTAAGTCTGGTTTGATAAGTTCCGACATTATTGAGTTTTCGACTCTGTCTGGAATTTTAACAGCTAAGAAGGTTCTAGATGTTAAGACAGCCAATGGTGAAGCACAAGAGTCGTCCTATTTCATTGAACTGAACTTTCCTGCCGCCCCGTCTAATGAATTCAACTCTGATGAGGTTTCGGTTATCTCCAAGGCCTTGAACAGCTCTTGTATTATTGATATAAGGAGGGCAACTGTCACAGATGATCTACTTGTTGTGCTTCCATCAGCAAAAGCGGTTGCAGATTTACAGCCACAGCCTGATGCAATCAAAAAATGTCCGGGGTCTAGGGGAGTGCTTGCAACAGCGATTGCTCCTCCAGAGTCCGGTTATGATTTTTACAGTAGATTCTTTGCCCCTAAACACGGTGTTAATGAGGATCCTGTTTGCGGGAGCGCACATTGTGTCTTAGCATCATACTGGTGCAAAAAACTCGGAAAATCTGATGTTGTTGGATACGTGGCATCACGTCGAGGAGGAGTAGTATGCATTCATCTAGACGAGCAGAATCAAAGGGTGCTGCTGCGAGGGAAAGCGGTTACTGTCATGGAAGGAACTGTTTTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PhzC-PhzF

Protein Analysis

312

Amino Acids

33.89

Weight (kDa)

5.9

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhzC-PhzF PF02567 30 - 307 2e-81 Phenazine biosynthesis-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 95
AciI CCGC 6 cut(s) 131, 294, 471, 602, 756, 902
AclWI GGATC 4 cut(s) 3, 16, 740, 753
AcsI RAATTY 2 cut(s) 381, 485
AdeI CACNNNGTG 2 cut(s) 33, 770
AfiI CCNNNNNNNGG 7 cut(s) 18, 19, 130, 251, 692, 731, 755
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 5 cut(s) 115, 193, 455, 490, 526
AjiI CACGTC 2 cut(s) 207, 836
AluBI AGCT 7 cut(s) 25, 176, 242, 289, 311, 392, 531
AluI AGCT 7 cut(s) 25, 176, 242, 289, 311, 392, 531
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 150, 260
AlwI GGATC 4 cut(s) 3, 16, 740, 753
AoxI GGCC 2 cut(s) 180, 519
ApeKI GCWGC 3 cut(s) 128, 886, 889
ApoI RAATTY 2 cut(s) 381, 485
AspLEI GCGC 1 cut(s) 764
AsuC2I CCSGG 1 cut(s) 648
AsuHPI GGTGA 1 cut(s) 437
BamHI GGATCC 2 cut(s) 8, 745
BanII GRGCYC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 3 cut(s) 140, 873, 876
BccI CCATC 2 cut(s) 255, 598
BcgI CGANNNNNNTGC 6 cut(s) 44, 78, 420, 454, 781, 815
BciVI GTATCC 2 cut(s) 74, 815
BcnI CCSGG 1 cut(s) 648
BcoDI GTCTC 2 cut(s) 150, 260
BfaI CTAG 3 cut(s) 404, 654, 863
BfuI GTATCC 2 cut(s) 74, 815
BisI GCNGC 4 cut(s) 129, 471, 887, 890
BlsI GCNGC 4 cut(s) 130, 472, 888, 891
Bme1390I CCNGG 1 cut(s) 648
BmgBI CACGTC 2 cut(s) 207, 836
BmiI GGNNCC 3 cut(s) 10, 122, 747
BmrFI CCNGG 1 cut(s) 648
BmsI GCATC 4 cut(s) 51, 619, 788, 839
BpmI CTGGAG 2 cut(s) 164, 669
Bpu10I CCTNAGC 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 648
BsaAI YACGTR 1 cut(s) 826
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 3 cut(s) 245, 516, 647
BsaWI WCCGGW 1 cut(s) 692
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 7 cut(s) 18, 19, 130, 251, 692, 731, 755
Bse1I ACTGG 3 cut(s) 54, 163, 791
BseDI CCNNGG 3 cut(s) 245, 516, 647
BseLI CCNNNNNNNGG 7 cut(s) 18, 19, 130, 251, 692, 731, 755
BseMII CTCAG 2 cut(s) 252, 271
BseNI ACTGG 3 cut(s) 54, 163, 791
BseRI GAGGAG 4 cut(s) 234, 672, 855, 858
BseXI GCAGC 3 cut(s) 140, 873, 876
BseYI CCCAGC 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 2 cut(s) 182, 521
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 2 cut(s) 647, 693
BslI CCNNNNNNNGG 7 cut(s) 18, 19, 130, 251, 692, 731, 755
BsmAI GTCTC 2 cut(s) 150, 260
BsmBI CGTCTC 1 cut(s) 150
BsmI GAATGC 1 cut(s) 855
BsnI GGCC 2 cut(s) 182, 521
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 3 cut(s) 8, 571, 745
BspACI CCGC 6 cut(s) 131, 294, 471, 602, 756, 902
BspANI GGCC 2 cut(s) 182, 521
BspCNI CTCAG 2 cut(s) 251, 270
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 3 cut(s) 10, 122, 747
BspPI GGATC 4 cut(s) 3, 16, 740, 753
BspTNI GGTCTC 1 cut(s) 260
BsrI ACTGG 3 cut(s) 54, 163, 791
BssECI CCNNGG 3 cut(s) 245, 516, 647
BssMI GATC 3 cut(s) 8, 571, 745
BssT1I CCWWGG 1 cut(s) 516
Bst4CI ACNGT 5 cut(s) 562, 710, 734, 910, 925
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 2 cut(s) 140, 666
BstDEI CTNAG 5 cut(s) 38, 238, 257, 393, 775
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 764
BstKTI GATC 3 cut(s) 11, 574, 748
BstMAI GTCTC 2 cut(s) 150, 260
BstMBI GATC 3 cut(s) 8, 571, 745
BstSCI CCNGG 1 cut(s) 646
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 3 cut(s) 140, 873, 876
BstX2I RGATCY 2 cut(s) 8, 745
BstYI RGATCY 2 cut(s) 8, 745
BsuI GTATCC 2 cut(s) 74, 815
BsuRI GGCC 2 cut(s) 182, 521
BtrI CACGTC 2 cut(s) 207, 836
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 140, 666
CfoI GCGC 1 cut(s) 764
CseI GACGC 1 cut(s) 101
CviAII CATG 3 cut(s) 85, 299, 913
DdeI CTNAG 5 cut(s) 38, 238, 257, 393, 775
DpnI GATC 3 cut(s) 10, 573, 747
DpnII GATC 3 cut(s) 8, 571, 745
DraIII CACNNNGTG 2 cut(s) 33, 770
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 516
Eco147I AGGCCT 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoRI GAATTC 1 cut(s) 485
EcoT14I CCWWGG 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 857
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 516
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 3 cut(s) 88, 302, 916
FaiI YATR 8 cut(s) 47, 86, 300, 548, 699, 784, 853, 914
FatI CATG 3 cut(s) 84, 298, 912
FauI CCCGC 1 cut(s) 749
Fnu4HI GCNGC 4 cut(s) 129, 471, 887, 890
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 4 cut(s) 129, 471, 887, 890
FspBI CTAG 3 cut(s) 404, 654, 863
GlaI GCGC 1 cut(s) 763
GluI GCNGC 4 cut(s) 129, 471, 887, 890
GsaI CCCAGC 1 cut(s) 128
GsuI CTGGAG 2 cut(s) 164, 669
HaeIII GGCC 2 cut(s) 182, 521
HapII CCGG 2 cut(s) 647, 693
HgaI GACGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 764
Hin1II CATG 3 cut(s) 88, 302, 916
Hin6I GCGC 1 cut(s) 762
HinP1I GCGC 1 cut(s) 762
HinfI GANTC 9 cut(s) 104, 217, 222, 230, 370, 437, 689, 714, 874
HpaII CCGG 2 cut(s) 647, 693
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 2 cut(s) 91, 99
Hpy188I TCNGA 7 cut(s) 66, 74, 260, 352, 496, 803, 811
Hpy188III TCNNGA 4 cut(s) 378, 404, 686, 863
Hpy8I GTNNAC 2 cut(s) 91, 99
Hpy99I CGWCG 1 cut(s) 840
HpyAV CCTTC 3 cut(s) 193, 391, 911
HpyCH4III ACNGT 5 cut(s) 562, 710, 734, 910, 925
HpyCH4IV ACGT 3 cut(s) 206, 825, 835
HpyCH4V TGCA 6 cut(s) 55, 608, 632, 668, 792, 855
HpyF3I CTNAG 5 cut(s) 38, 238, 257, 393, 775
HpySE526I ACGT 3 cut(s) 206, 825, 835
Hsp92II CATG 3 cut(s) 88, 302, 916
HspAI GCGC 1 cut(s) 762
Kzo9I GATC 3 cut(s) 8, 571, 745
LmnI GCTCC 4 cut(s) 22, 247, 685, 759
Lsp1109I GCAGC 3 cut(s) 140, 873, 876
LweI GCATC 4 cut(s) 51, 619, 788, 839
MaeI CTAG 3 cut(s) 404, 654, 863
MaeII ACGT 3 cut(s) 206, 825, 835
MaeIII GTNAC 2 cut(s) 562, 904
MalI GATC 3 cut(s) 10, 573, 747
MboI GATC 3 cut(s) 8, 571, 745
MboII GAAGA 2 cut(s) 161, 306
MflI RGATCY 2 cut(s) 8, 745
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 2 cut(s) 381, 485
MluI ACGCGT 1 cut(s) 93
MlyI GAGTC 6 cut(s) 98, 211, 224, 364, 446, 698
MmeI TCCRAC 3 cut(s) 58, 375, 799
Mph1103I ATGCAT 1 cut(s) 857
MseI TTAA 4 cut(s) 330, 386, 411, 738
MslI CAYNNNNRTG 2 cut(s) 50, 787
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 2 cut(s) 647, 693
MspR9I CCNGG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 855
MvnI CGCG 1 cut(s) 95
NciI CCSGG 1 cut(s) 648
NdeII GATC 3 cut(s) 8, 571, 745
NlaIII CATG 3 cut(s) 88, 302, 916
NlaIV GGNNCC 3 cut(s) 10, 122, 747
NmeAIII GCCGAG 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 562
NsiI ATGCAT 1 cut(s) 857
PceI AGGCCT 1 cut(s) 521
PcsI WCGNNNNNNNCGW 1 cut(s) 70
PctI GAATGC 1 cut(s) 855
PfeI GAWTC 3 cut(s) 222, 714, 874
PflFI GACNNNGTC 1 cut(s) 373
PkrI GCNGC 4 cut(s) 130, 472, 888, 891
PleI GAGTC 6 cut(s) 98, 211, 224, 364, 445, 697
PpsI GAGTC 6 cut(s) 98, 211, 224, 364, 445, 697
Ppu21I YACGTR 1 cut(s) 826
Psp124BI GAGCTC 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 10, 122, 747
PsuI RGATCY 2 cut(s) 8, 745
PsyI GACNNNGTC 1 cut(s) 373
RseI CAYNNNNRTG 2 cut(s) 50, 787
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 4 cut(s) 330, 386, 411, 738
SatI GCNGC 4 cut(s) 129, 471, 887, 890
Sau3AI GATC 3 cut(s) 8, 571, 745
SchI GAGTC 6 cut(s) 98, 211, 224, 364, 446, 698
ScrFI CCNGG 1 cut(s) 648
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 4 cut(s) 51, 619, 788, 839
SmiMI CAYNNNNRTG 2 cut(s) 50, 787
Sse9I AATT 2 cut(s) 381, 485
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 6 cut(s) 131, 294, 471, 602, 756, 902
SspMI CTAG 3 cut(s) 404, 654, 863
SstI GAGCTC 1 cut(s) 27
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 646
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 5 cut(s) 562, 710, 734, 910, 925
TaiI ACGT 3 cut(s) 209, 828, 838
TaqI TCGA 4 cut(s) 186, 220, 368, 838
TaqII GACCGA 1 cut(s) 242
TasI AATT 2 cut(s) 381, 485
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 3 cut(s) 222, 714, 874
Tru1I TTAA 4 cut(s) 330, 386, 411, 738
Tru9I TTAA 4 cut(s) 330, 386, 411, 738
TscAI CASTG 1 cut(s) 170
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 3 cut(s) 128, 886, 889
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 3 cut(s) 439, 498, 848
TspRI CASTG 1 cut(s) 170
Tth111I GACNNNGTC 1 cut(s) 373
XapI RAATTY 2 cut(s) 381, 485
XbaI TCTAGA 2 cut(s) 403, 862
XspI CTAG 3 cut(s) 404, 654, 863
Zsp2I ATGCAT 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.