MD03G1267600.v1.1

Phenazine biosynthesis-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001362
Physical Location & Seq
Reverse (-)
35258515 .. 35263848
5334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1267600.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCAGGTTCAAGATCCTCTCCGTCGATTGTTGCTCCATGGTGTTTGTGAGGTGGACGCGTTCACTGACTCAGCTTTCAAGGGCAACCCAGCAGCGGTTTGCTCGCTGGAGGAAGACAGAGACGACCAGTGGTTGCAGGCGGTGGCCGCCGAGTTCAATCTCTCCGTGACATGTTACTTGACTCGGCTCCTCGGTTCGGTCTCAGATGGTTCACTCCTTCCCTTGAGGTCTCAGCTCACCTCTCTTCAATTCAATGGTTTTGCTAAGCTTTGTGGTCATGCAACATTAGCAGCTGCATACACTCTCTTCAACTCTGGCTTGATAAAGTCCGACATCGTTGAGTTTGCCACATTATCCGGAATCCTAACAGCTAAAAAGGTCCTAGATGTTAAGACGGCCAACGGTTTGAATACTCAAAATGGTGAAGCGCAAGAATCCTTTTCTGTTGAGCTGAATTTTCCTGCCGACCCCTCTAATCAATTCAATTCCGGTGATGTTTCGGTGATCTCCAAGGCTTTGGGTGGTGCTTCTATGGTTGAAATAAGGAGGACAACCGTCACAGATGATTTACTTGTTGTGCTTCCATCAGCACAAGCTGTCACAGATTTACAGCCGAAGTTAGATGCAATTCGACAATGTCCAGGGTCCAGGGGCGTGATTGTAACTGGGATTGCTCCTCCGGAATCTGGATATGATTTTTACAGTAGATTCTTCTGCCCCAAACATGGAATTGATGAGGATCCCGTTTGTGGGAGCGCACATTGTGCCCTAGCATCATACTGGTGCAAAAAACTTGGAAAGTCTGATGTTTTTGCTTATCAGGCATCACCTCGAGGGGGAGCAATTAACATTCATCTAGATGAGCAAAACCAGAGGGTACTCTGCGAGGAAAAGCTGTTCTTGTTGTGCTTCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PhzC-PhzF

Protein Analysis

306

Amino Acids

32.96

Weight (kDa)

5.15

Isoelectric Point (pI)

30.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhzC-PhzF PF02567 18 - 293 6.8e-65 Phenazine biosynthesis-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 831
AccII CGCG 1 cut(s) 59
AccIII TCCGGA 2 cut(s) 356, 679
AciI CCGC 3 cut(s) 95, 140, 147
AclWI GGATC 3 cut(s) 8, 734, 747
AcoI YGGCCR 2 cut(s) 144, 396
AcsI RAATTY 1 cut(s) 454
AdeI CACNNNGTG 1 cut(s) 764
AfaI GTAC 1 cut(s) 879
AfiI CCNNNNNNNGG 7 cut(s) 94, 196, 686, 725, 749, 750, 836
AflIII ACRYGT 2 cut(s) 57, 170
AgsI TTSAA 9 cut(s) 11, 79, 157, 248, 253, 310, 409, 484, 539
AjnI CCWGG 2 cut(s) 640, 647
AloI GAACNNNNNNTCC 2 cut(s) 881, 913
AluBI AGCT 8 cut(s) 74, 235, 268, 293, 371, 451, 596, 895
AluI AGCT 8 cut(s) 74, 235, 268, 293, 371, 451, 596, 895
Alw26I GTCTC 3 cut(s) 114, 205, 234
AlwI GGATC 3 cut(s) 8, 734, 747
Ama87I CYCGRG 1 cut(s) 831
Aor13HI TCCGGA 2 cut(s) 356, 679
AoxI GGCC 2 cut(s) 144, 396
ApeKI GCWGC 3 cut(s) 92, 290, 293
ApoI RAATTY 1 cut(s) 454
AspLEI GCGC 2 cut(s) 430, 758
AspS9I GGNCC 2 cut(s) 379, 645
AsuHPI GGTGA 5 cut(s) 229, 434, 503, 514, 819
AvaI CYCGRG 1 cut(s) 831
AvaII GGWCC 2 cut(s) 379, 645
BaeGI GKGCMC 1 cut(s) 769
BamHI GGATCC 1 cut(s) 739
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 3 cut(s) 104, 280, 302
BccI CCATC 2 cut(s) 200, 592
BceAI ACGGC 1 cut(s) 411
BciT130I CCWGG 2 cut(s) 642, 649
BcoDI GTCTC 3 cut(s) 114, 205, 234
BfaI CTAG 3 cut(s) 383, 770, 857
BisI GCNGC 4 cut(s) 93, 147, 291, 294
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 4 cut(s) 94, 148, 292, 295
Bme1390I CCNGG 2 cut(s) 642, 649
Bme18I GGWCC 2 cut(s) 379, 645
BmeT110I CYCGRG 1 cut(s) 831
BmgT120I GGNCC 2 cut(s) 379, 645
BmiI GGNNCC 3 cut(s) 188, 646, 741
BmrFI CCNGG 2 cut(s) 642, 649
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 3 cut(s) 613, 782, 833
BmuI ACTGGG 1 cut(s) 675
BoxI GACNNNNGTC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 128
Bpu1102I GCTNAGC 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 205, 234
BsaJI CCNNGG 5 cut(s) 37, 190, 510, 641, 648
BsaWI WCCGGW 3 cut(s) 356, 488, 679
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 196, 686, 725, 749, 750, 836
Bse1I ACTGG 3 cut(s) 127, 670, 785
Bse8I GATNNNNATC 1 cut(s) 738
BseAI TCCGGA 2 cut(s) 356, 679
BseBI CCWGG 2 cut(s) 642, 649
BseDI CCNNGG 5 cut(s) 37, 190, 510, 641, 648
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 7 cut(s) 94, 196, 686, 725, 749, 750, 836
BseMII CTCAG 3 cut(s) 84, 216, 245
BseNI ACTGG 3 cut(s) 127, 670, 785
BseRI GAGGAG 2 cut(s) 179, 666
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 3 cut(s) 104, 280, 302
BseYI CCCAGC 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 146, 398
BsiHKCI CYCGRG 1 cut(s) 831
BsiSI CCGG 3 cut(s) 357, 489, 680
BslI CCNNNNNNNGG 7 cut(s) 94, 196, 686, 725, 749, 750, 836
BsmAI GTCTC 3 cut(s) 114, 205, 234
BsmBI CGTCTC 1 cut(s) 114
BsnI GGCC 2 cut(s) 146, 398
Bso31I GGTCTC 2 cut(s) 205, 234
BsoBI CYCGRG 1 cut(s) 831
Bsp1286I GDGCHC 1 cut(s) 769
Bsp13I TCCGGA 2 cut(s) 356, 679
Bsp143I GATC 3 cut(s) 13, 504, 739
Bsp1720I GCTNAGC 1 cut(s) 264
Bsp19I CCATGG 1 cut(s) 37
BspACI CCGC 3 cut(s) 95, 140, 147
BspANI GGCC 2 cut(s) 146, 398
BspCNI CTCAG 3 cut(s) 83, 215, 244
BspEI TCCGGA 2 cut(s) 356, 679
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 3 cut(s) 188, 646, 741
BspPI GGATC 3 cut(s) 8, 734, 747
BspTNI GGTCTC 2 cut(s) 205, 234
BsrI ACTGG 3 cut(s) 127, 670, 785
BssECI CCNNGG 5 cut(s) 37, 190, 510, 641, 648
BssMI GATC 3 cut(s) 13, 504, 739
BssT1I CCWWGG 2 cut(s) 37, 510
Bst2UI CCWGG 2 cut(s) 642, 649
Bst4CI ACNGT 3 cut(s) 404, 556, 704
Bst6I CTCTTC 2 cut(s) 249, 311
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 2 cut(s) 104, 138
BstDEI CTNAG 4 cut(s) 70, 202, 231, 264
BstDSI CCRYGG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 59
BstHHI GCGC 2 cut(s) 430, 758
BstKTI GATC 3 cut(s) 16, 507, 742
BstMAI GTCTC 3 cut(s) 114, 205, 234
BstMBI GATC 3 cut(s) 13, 504, 739
BstMWI GCNNNNNNNGC 4 cut(s) 146, 287, 764, 821
BstNI CCWGG 2 cut(s) 642, 649
BstNSI RCATGY 1 cut(s) 174
BstPAI GACNNNNGTC 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 640, 647
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 3 cut(s) 104, 280, 302
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 2 cut(s) 13, 739
BstXI CCANNNNNNTGG 1 cut(s) 517
BstYI RGATCY 2 cut(s) 13, 739
BsuRI GGCC 2 cut(s) 146, 398
BtgI CCRYGG 1 cut(s) 37
BtsIMutI CAGTG 2 cut(s) 63, 134
Cac8I GCNNGC 2 cut(s) 104, 138
CfoI GCGC 2 cut(s) 430, 758
Cfr13I GGNCC 2 cut(s) 379, 645
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 878
CviAII CATG 4 cut(s) 38, 171, 278, 725
CviQI GTAC 1 cut(s) 878
DdeI CTNAG 4 cut(s) 70, 202, 231, 264
DpnI GATC 3 cut(s) 15, 506, 741
DpnII GATC 3 cut(s) 13, 504, 739
DraIII CACNNNGTG 1 cut(s) 764
EaeI YGGCCR 2 cut(s) 144, 396
Eam1104I CTCTTC 2 cut(s) 249, 311
EarI CTCTTC 2 cut(s) 249, 311
Eco130I CCWWGG 2 cut(s) 37, 510
Eco31I GGTCTC 2 cut(s) 205, 234
Eco47I GGWCC 2 cut(s) 379, 645
Eco88I CYCGRG 1 cut(s) 831
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 640, 647
EcoT14I CCWWGG 2 cut(s) 37, 510
ErhI CCWWGG 2 cut(s) 37, 510
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 4 cut(s) 41, 174, 281, 728
FaiI YATR 8 cut(s) 39, 172, 279, 298, 533, 693, 726, 778
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FatI CATG 4 cut(s) 37, 170, 277, 724
Fnu4HI GCNGC 4 cut(s) 93, 147, 291, 294
Fsp4HI GCNGC 4 cut(s) 93, 147, 291, 294
FspBI CTAG 3 cut(s) 383, 770, 857
GlaI GCGC 2 cut(s) 429, 757
GluI GCNGC 4 cut(s) 93, 147, 291, 294
GsaI CCCAGC 1 cut(s) 92
GsuI CTGGAG 1 cut(s) 128
HaeIII GGCC 2 cut(s) 146, 398
HapII CCGG 3 cut(s) 357, 489, 680
HgaI GACGC 1 cut(s) 65
HhaI GCGC 2 cut(s) 430, 758
Hin1II CATG 4 cut(s) 41, 174, 281, 728
Hin6I GCGC 2 cut(s) 428, 756
HinP1I GCGC 2 cut(s) 428, 756
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 6 cut(s) 68, 181, 360, 434, 683, 708
HpaII CCGG 3 cut(s) 357, 489, 680
HphI GGTGA 5 cut(s) 229, 434, 503, 514, 819
Hpy166II GTNNAC 3 cut(s) 55, 63, 212
Hpy188I TCNGA 3 cut(s) 205, 331, 805
Hpy188III TCNNGA 5 cut(s) 11, 357, 680, 687, 857
Hpy8I GTNNAC 3 cut(s) 55, 63, 212
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 3 cut(s) 404, 556, 704
HpyCH4V TGCA 6 cut(s) 4, 136, 281, 296, 626, 786
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 287, 764, 821
HpyF3I CTNAG 4 cut(s) 70, 202, 231, 264
Hsp92II CATG 4 cut(s) 41, 174, 281, 728
HspAI GCGC 2 cut(s) 428, 756
Kpn2I TCCGGA 2 cut(s) 356, 679
Kzo9I GATC 3 cut(s) 13, 504, 739
LmnI GCTCC 5 cut(s) 39, 192, 679, 753, 839
Lsp1109I GCAGC 3 cut(s) 104, 280, 302
LweI GCATC 3 cut(s) 613, 782, 833
MaeI CTAG 3 cut(s) 383, 770, 857
MaeIII GTNAC 5 cut(s) 166, 173, 556, 598, 661
MalI GATC 3 cut(s) 15, 506, 741
MboI GATC 3 cut(s) 13, 504, 739
MboII GAAGA 4 cut(s) 125, 236, 298, 703
MflI RGATCY 2 cut(s) 13, 739
MhlI GDGCHC 1 cut(s) 769
MluCI AATT 7 cut(s) 248, 454, 479, 484, 627, 729, 843
MluI ACGCGT 1 cut(s) 57
MlyI GAGTC 2 cut(s) 62, 175
MmeI TCCRAC 1 cut(s) 354
MroI TCCGGA 2 cut(s) 356, 679
MseI TTAA 2 cut(s) 390, 846
MslI CAYNNNNRTG 2 cut(s) 781, 858
MspA1I CMGCKG 2 cut(s) 95, 293
MspI CCGG 3 cut(s) 357, 489, 680
MspR9I CCNGG 2 cut(s) 642, 649
MvaI CCWGG 2 cut(s) 642, 649
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 4 cut(s) 146, 287, 764, 821
NcoI CCATGG 1 cut(s) 37
NdeII GATC 3 cut(s) 13, 504, 739
NlaIII CATG 4 cut(s) 41, 174, 281, 728
NlaIV GGNNCC 3 cut(s) 188, 646, 741
NmeAIII GCCGAG 2 cut(s) 163, 175
NmuCI GTSAC 3 cut(s) 166, 556, 598
NspI RCATGY 1 cut(s) 174
PaeR7I CTCGAG 1 cut(s) 831
PciI ACATGT 1 cut(s) 170
PfeI GAWTC 4 cut(s) 360, 434, 683, 708
PflFI GACNNNGTC 1 cut(s) 636
PkrI GCNGC 4 cut(s) 94, 148, 292, 295
PleI GAGTC 2 cut(s) 62, 175
PpsI GAGTC 2 cut(s) 62, 175
PpuMI RGGWCCY 1 cut(s) 379
PscI ACATGT 1 cut(s) 170
PshAI GACNNNNGTC 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 640, 647
PspFI CCCAGC 1 cut(s) 88
PspGI CCWGG 2 cut(s) 640, 647
PspN4I GGNNCC 3 cut(s) 188, 646, 741
PspPI GGNCC 2 cut(s) 379, 645
PspPPI RGGWCCY 1 cut(s) 379
PspXI VCTCGAGB 1 cut(s) 831
PsuI RGATCY 2 cut(s) 13, 739
PsyI GACNNNGTC 1 cut(s) 636
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 879
RsaNI GTAC 1 cut(s) 878
RseI CAYNNNNRTG 2 cut(s) 781, 858
SaqAI TTAA 2 cut(s) 390, 846
SatI GCNGC 4 cut(s) 93, 147, 291, 294
Sau3AI GATC 3 cut(s) 13, 504, 739
Sau96I GGNCC 2 cut(s) 379, 645
SchI GAGTC 2 cut(s) 62, 175
ScrFI CCNGG 2 cut(s) 642, 649
SduI GDGCHC 1 cut(s) 769
SfaNI GCATC 3 cut(s) 613, 782, 833
Sfr274I CTCGAG 1 cut(s) 831
SinI GGWCC 2 cut(s) 379, 645
SlaI CTCGAG 1 cut(s) 831
SmiMI CAYNNNNRTG 2 cut(s) 781, 858
SmlI CTYRAG 2 cut(s) 223, 831
SmoI CTYRAG 2 cut(s) 223, 831
Sse9I AATT 7 cut(s) 248, 454, 479, 484, 627, 729, 843
SsiI CCGC 3 cut(s) 95, 140, 147
SspMI CTAG 3 cut(s) 383, 770, 857
StyD4I CCNGG 2 cut(s) 640, 647
StyI CCWWGG 2 cut(s) 37, 510
TaaI ACNGT 3 cut(s) 404, 556, 704
TaqI TCGA 3 cut(s) 25, 631, 832
TaqII GACCGA 1 cut(s) 187
TasI AATT 7 cut(s) 248, 454, 479, 484, 627, 729, 843
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 4 cut(s) 360, 434, 683, 708
Tru1I TTAA 2 cut(s) 390, 846
Tru9I TTAA 2 cut(s) 390, 846
TscAI CASTG 2 cut(s) 70, 134
TseFI GTSAC 3 cut(s) 166, 556, 598
TseI GCWGC 3 cut(s) 92, 290, 293
Tsp45I GTSAC 3 cut(s) 166, 556, 598
TspDTI ATGAA 1 cut(s) 842
TspGWI ACGGA 2 cut(s) 11, 154
TspRI CASTG 2 cut(s) 70, 134
Tth111I GACNNNGTC 1 cut(s) 636
VpaK11BI GGWCC 2 cut(s) 379, 645
XapI RAATTY 1 cut(s) 454
XbaI TCTAGA 1 cut(s) 856
XceI RCATGY 1 cut(s) 174
XhoI CTCGAG 1 cut(s) 831
XspI CTAG 3 cut(s) 383, 770, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.