MD04G1145300.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004213
Physical Location & Seq
Forward (+)
23418850 .. 23420152
1303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1145300.v1.1.491

Sequence Viewer

Length: 564 bp
ATGGGGTGCAAGCGTAAGCTCCGGGCATCTACAAGTTGTCAGGCTGAAGGAACTTCCTCAGAAAGTGGAATAGACAGATTTAGTGATCTTCCGGAGGAAGTTGTTCATCGTATTCTTTCCTTACTCAATTTCAGGGACCTCACTCGAGTGGGTGGTCTGTCCAAAAGATGCAGACAATTTCATCTATCAGTTCCAGTAGTCGATTTTAATTCAAATTGGGTGCCACTAACAAAAACTGTTAAGTGTTTTGCGAGGCTCATGAGTTCTTTTGATAGATACTTTCTTGATCGTGGTCATAACAGGTTGCAGCGACTTGACATTGACTGGAGTTTCTTACAAGTGAAACAGCGAAGAAAGTTTCTGATTCGTAGTGCATTAACGTGTAATGTTGAAGACTTGATCTTTATTTCTGAAATTGTGGGACCAATATGTTTTCGTTGCCATCTTTCATCTTCCATTCTCAATCTTTGCGCTCTCTATCAGTGCGCTTGCATATGTTCTGTACGGGGGTTGTTGAAGCGCCATCTCTATCCTTTTCTAGTAATCTCCATTGCTTATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

188

Amino Acids

21.55

Weight (kDa)

9.47

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 27 - 59 3.2e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 220
AccIII TCCGGA 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 1 cut(s) 504
AflIII ACRYGT 1 cut(s) 380
AgsI TTSAA 3 cut(s) 213, 392, 517
AleI CACNNNNGTG 1 cut(s) 146
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 144
Aor13HI TCCGGA 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 307
Asp700I GAANNNNTTC 1 cut(s) 102
AspLEI GCGC 3 cut(s) 473, 488, 522
AspS9I GGNCC 2 cut(s) 136, 422
AsuC2I CCSGG 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 136, 422
BanI GGYRCC 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 319
BccI CCATC 2 cut(s) 450, 531
BcnI CCSGG 1 cut(s) 23
BfaI CTAG 1 cut(s) 539
BfoI RGCGCY 1 cut(s) 523
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 23
Bme18I GGWCC 2 cut(s) 136, 422
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 136, 422
BmiI GGNNCC 3 cut(s) 137, 222, 423
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 2 cut(s) 35, 158
BpiI GAAGAC 1 cut(s) 399
BpmI CTGGAG 1 cut(s) 346
BpuMI CCSGG 1 cut(s) 23
BsaWI WCCGGW 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 194, 329
Bse3DI GCAATG 1 cut(s) 549
BseAI TCCGGA 1 cut(s) 91
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 194, 329
BseXI GCAGC 1 cut(s) 319
BshNI GGYRCC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 2 cut(s) 22, 92
BslFI GGGAC 2 cut(s) 149, 435
BsmFI GGGAC 2 cut(s) 149, 435
BsoBI CYCGRG 1 cut(s) 144
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 3 cut(s) 85, 286, 399
BspCNI CTCAG 1 cut(s) 71
BspEI TCCGGA 1 cut(s) 91
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 137, 222, 423
BspT107I GGYRCC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 549
BsrI ACTGG 2 cut(s) 194, 329
BssMI GATC 3 cut(s) 85, 286, 399
Bst4CI ACNGT 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 11, 490
BstDEI CTNAG 1 cut(s) 58
BstH2I RGCGCY 1 cut(s) 523
BstHHI GCGC 3 cut(s) 473, 488, 522
BstKTI GATC 3 cut(s) 88, 289, 402
BstMBI GATC 3 cut(s) 85, 286, 399
BstSCI CCNGG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 319
BstV2I GAAGAC 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 488
Cac8I GCNNGC 2 cut(s) 11, 490
CciI TCATGA 1 cut(s) 258
CfoI GCGC 3 cut(s) 473, 488, 522
Cfr13I GGNCC 2 cut(s) 136, 422
Csp6I GTAC 1 cut(s) 503
CviAII CATG 1 cut(s) 259
CviJI RGCY 3 cut(s) 19, 44, 256
CviKI_1 RGCY 3 cut(s) 19, 44, 256
CviQI GTAC 1 cut(s) 503
DdeI CTNAG 1 cut(s) 58
DpnI GATC 3 cut(s) 87, 288, 401
DpnII GATC 3 cut(s) 85, 286, 399
Eco47I GGWCC 2 cut(s) 136, 422
Eco57I CTGAAG 1 cut(s) 66
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 136
FaeI CATG 1 cut(s) 262
FaiI YATR 5 cut(s) 260, 297, 430, 494, 496
FaqI GGGAC 2 cut(s) 149, 435
FatI CATG 1 cut(s) 258
FauNDI CATATG 1 cut(s) 494
Fnu4HI GCNGC 1 cut(s) 308
Fsp4HI GCNGC 1 cut(s) 308
FspBI CTAG 1 cut(s) 539
GlaI GCGC 3 cut(s) 472, 487, 521
GluI GCNGC 1 cut(s) 308
GsuI CTGGAG 1 cut(s) 346
HaeII RGCGCY 1 cut(s) 523
HapII CCGG 2 cut(s) 22, 92
HhaI GCGC 3 cut(s) 473, 488, 522
Hin1II CATG 1 cut(s) 262
Hin6I GCGC 3 cut(s) 471, 486, 520
HinP1I GCGC 3 cut(s) 471, 486, 520
HinfI GANTC 1 cut(s) 364
HpaII CCGG 2 cut(s) 22, 92
Hpy188I TCNGA 3 cut(s) 61, 363, 412
Hpy188III TCNNGA 3 cut(s) 92, 259, 284
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 5 cut(s) 9, 171, 307, 374, 492
HpyF3I CTNAG 1 cut(s) 58
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 1 cut(s) 262
HspAI GCGC 3 cut(s) 471, 486, 520
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 85, 286, 399
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 7 cut(s) 26, 35, 105, 118, 207, 286, 310
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 2 cut(s) 35, 158
MaeI CTAG 1 cut(s) 539
MaeII ACGT 1 cut(s) 380
MalI GATC 3 cut(s) 87, 288, 401
MboI GATC 3 cut(s) 85, 286, 399
MboII GAAGA 4 cut(s) 80, 363, 404, 444
MluCI AATT 5 cut(s) 127, 176, 208, 214, 414
MnlI CCTC 4 cut(s) 67, 88, 149, 246
MroI TCCGGA 1 cut(s) 91
MroXI GAANNNNTTC 1 cut(s) 102
MseI TTAA 3 cut(s) 207, 240, 377
MslI CAYNNNNRTG 2 cut(s) 146, 379
MspI CCGG 2 cut(s) 22, 92
MspR9I CCNGG 1 cut(s) 23
NciI CCSGG 1 cut(s) 23
NdeI CATATG 1 cut(s) 494
NdeII GATC 3 cut(s) 85, 286, 399
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 3 cut(s) 137, 222, 423
OliI CACNNNNGTG 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 144
PagI TCATGA 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 1 cut(s) 364
PkrI GCNGC 1 cut(s) 309
PpuMI RGGWCCY 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 137, 222, 423
PspPI GGNCC 2 cut(s) 136, 422
PspPPI RGGWCCY 1 cut(s) 136
PspXI VCTCGAGB 1 cut(s) 144
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
RseI CAYNNNNRTG 2 cut(s) 146, 379
SaqAI TTAA 3 cut(s) 207, 240, 377
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 3 cut(s) 85, 286, 399
Sau96I GGNCC 2 cut(s) 136, 422
ScrFI CCNGG 1 cut(s) 23
SetI ASST 4 cut(s) 21, 141, 305, 383
SfaNI GCATC 2 cut(s) 35, 158
Sfr274I CTCGAG 1 cut(s) 144
SinI GGWCC 2 cut(s) 136, 422
SlaI CTCGAG 1 cut(s) 144
SmiMI CAYNNNNRTG 2 cut(s) 146, 379
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
Sse9I AATT 5 cut(s) 127, 176, 208, 214, 414
SspMI CTAG 1 cut(s) 539
StyD4I CCNGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 383
TaqI TCGA 2 cut(s) 145, 201
TasI AATT 5 cut(s) 127, 176, 208, 214, 414
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 3 cut(s) 207, 240, 377
Tru9I TTAA 3 cut(s) 207, 240, 377
TscAI CASTG 1 cut(s) 488
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 95, 170, 438
TspRI CASTG 1 cut(s) 488
VpaK11BI GGWCC 2 cut(s) 136, 422
XhoI CTCGAG 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 102
XspI CTAG 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.