Rorug02G0131100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004213
Physical Location & Seq
Forward (+)
11329928 .. 11330636
709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0131100.1

Sequence Viewer

Length: 612 bp
ATGGCTGACAGCCACAGGCACCGTCACCGAAACCAGCGACTAGTCCCTTCCGCCTGTCGACCTCGCATCGAATTCGATGATCTCCTCATTGAAATAATATCAAGACTCCCAGTCAAAACCCTACTTCAATTCCGATGCGTTTGCAAGTCATGGCGTGCTTTGATCTCCAGTTCTCACTTGGCTAGGAAGCACCTACGCTGCTTCAACGCCAACCGCCGCCGCAGGCTGCTTTTCTCAGTCGCAGCTTTCCAATCCATCGACATCAAAGCACTAAACGACGGAGTTTGCCACTCAAGCAGTAAGCTTTGTTTACGTATATCTGTTGGAGTTTTGAAAATTGTGGGTTCTTGCAACGGCTTGATTTGTTTACTGGGAATCTCGGATTTGGTTATCTTGTGGAACCCTAGTGCTAGAGTTGCCAGCAAAAGCAAAAGAAATGGTACTCTTGCAACCACAGTTGTGATGTTTCAGCTCAAAACAGAGACATGGAGGAGTATTGATGACCTCGATCATGTTAATTTGTATGGGCAGGGGTGCTTGATAATTGGTGCTCTACATTGGGTAGAGATGAAATGGGAAGAGGTGGTGATGATTCATCCGGTACTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

23.07

Weight (kDa)

9.9

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 60 2e-10 F-box domain
F-box-like PF12937 26 - 64 1.2e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 58
AciI CCGC 4 cut(s) 51, 214, 217, 220
AcsI RAATTY 1 cut(s) 71
AfaI GTAC 2 cut(s) 442, 603
AgsI TTSAA 4 cut(s) 92, 128, 205, 334
AhdI GACNNNNNGTC 1 cut(s) 110
AhlI ACTAGT 1 cut(s) 40
AleI CACNNNNGTG 1 cut(s) 458
AluBI AGCT 3 cut(s) 245, 304, 472
AluI AGCT 3 cut(s) 245, 304, 472
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 476
ApeKI GCWGC 3 cut(s) 198, 226, 242
ApoI RAATTY 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 17, 598
BanI GGYRCC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 3 cut(s) 185, 213, 254
BccI CCATC 1 cut(s) 263
BceAI ACGGC 1 cut(s) 370
BcgI CGANNNNNNTGC 4 cut(s) 55, 89, 123, 157
BcoDI GTCTC 1 cut(s) 476
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 183, 405, 411
BisI GCNGC 5 cut(s) 199, 217, 220, 227, 243
BlsI GCNGC 5 cut(s) 200, 218, 221, 228, 244
BmeRI GACNNNNNGTC 1 cut(s) 110
BmiI GGNNCC 2 cut(s) 20, 401
BmrI ACTGGG 2 cut(s) 104, 380
BmsI GCATC 2 cut(s) 75, 125
BmuI ACTGGG 2 cut(s) 104, 380
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 277
BsaAI YACGTR 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 598
Bse1I ACTGG 3 cut(s) 110, 168, 375
BseGI GGATG 1 cut(s) 595
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 3 cut(s) 110, 168, 375
BseRI GAGGAG 2 cut(s) 74, 505
BseXI GCAGC 3 cut(s) 185, 213, 254
BshNI GGYRCC 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 1 cut(s) 599
BslFI GGGAC 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 553
Bsp143I GATC 3 cut(s) 79, 162, 508
BspACI CCGC 4 cut(s) 51, 214, 217, 220
BspCNI CTCAG 1 cut(s) 248
BspLI GGNNCC 2 cut(s) 20, 401
BspT107I GGYRCC 1 cut(s) 18
BsrI ACTGG 3 cut(s) 110, 168, 375
BssMI GATC 3 cut(s) 79, 162, 508
Bst4CI ACNGT 2 cut(s) 23, 457
Bst6I CTCTTC 1 cut(s) 573
BstBAI YACGTR 1 cut(s) 314
BstC8I GCNNGC 3 cut(s) 156, 224, 421
BstDEI CTNAG 1 cut(s) 235
BstF5I GGATG 1 cut(s) 595
BstKTI GATC 3 cut(s) 82, 165, 511
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 3 cut(s) 79, 162, 508
BstMWI GCNNNNNNNGC 2 cut(s) 294, 416
BstSNI TACGTA 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 185, 213, 254
BtsCI GGATG 1 cut(s) 595
Cac8I GCNNGC 3 cut(s) 156, 224, 421
Csp6I GTAC 2 cut(s) 441, 602
CviAII CATG 3 cut(s) 150, 486, 512
CviJI RGCY 8 cut(s) 5, 12, 182, 226, 245, 304, 357, 472
CviKI_1 RGCY 8 cut(s) 5, 12, 182, 226, 245, 304, 357, 472
CviQI GTAC 2 cut(s) 441, 602
DdeI CTNAG 1 cut(s) 235
DpnI GATC 3 cut(s) 81, 164, 510
DpnII GATC 3 cut(s) 79, 162, 508
DriI GACNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 573
Eam1105I GACNNNNNGTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 573
EciI GGCGGA 1 cut(s) 40
Eco105I TACGTA 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 71
FaeI CATG 3 cut(s) 153, 489, 515
FaiI YATR 5 cut(s) 151, 317, 487, 513, 525
FaqI GGGAC 1 cut(s) 29
FatI CATG 3 cut(s) 149, 485, 511
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 5 cut(s) 199, 217, 220, 227, 243
FokI GGATG 1 cut(s) 582
Fsp4HI GCNGC 5 cut(s) 199, 217, 220, 227, 243
FspBI CTAG 4 cut(s) 41, 183, 405, 411
GluI GCNGC 5 cut(s) 199, 217, 220, 227, 243
GsuI CTGGAG 1 cut(s) 151
HapII CCGG 1 cut(s) 599
Hin1II CATG 3 cut(s) 153, 489, 515
HincII GTYRAC 1 cut(s) 59
HindII GTYRAC 1 cut(s) 59
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 3 cut(s) 105, 375, 592
HpaII CCGG 1 cut(s) 599
HphI GGTGA 2 cut(s) 17, 598
Hpy166II GTNNAC 3 cut(s) 59, 311, 368
Hpy188I TCNGA 2 cut(s) 134, 382
Hpy188III TCNNGA 1 cut(s) 102
Hpy8I GTNNAC 3 cut(s) 59, 311, 368
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 23, 457
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 144, 351, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 294, 416
HpyF3I CTNAG 1 cut(s) 235
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 3 cut(s) 153, 489, 515
Kzo9I GATC 3 cut(s) 79, 162, 508
LpnPI CCDG 8 cut(s) 47, 67, 123, 181, 208, 356, 433, 515
Lsp1109I GCAGC 3 cut(s) 185, 213, 254
LweI GCATC 2 cut(s) 75, 125
MaeI CTAG 4 cut(s) 41, 183, 405, 411
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 23
MalI GATC 3 cut(s) 81, 164, 510
MboI GATC 3 cut(s) 79, 162, 508
MboII GAAGA 1 cut(s) 590
MhlI GDGCHC 1 cut(s) 553
MluCI AATT 6 cut(s) 71, 128, 336, 517, 543, 607
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 5 cut(s) 72, 95, 483, 515, 574
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 1 cut(s) 458
MspI CCGG 1 cut(s) 599
MwoI GCNNNNNNNGC 2 cut(s) 294, 416
NdeII GATC 3 cut(s) 79, 162, 508
NlaIII CATG 3 cut(s) 153, 489, 515
NlaIV GGNNCC 2 cut(s) 20, 401
NmuCI GTSAC 1 cut(s) 23
OliI CACNNNNGTG 1 cut(s) 458
PfeI GAWTC 2 cut(s) 375, 592
PkrI GCNGC 5 cut(s) 200, 218, 221, 228, 244
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
Ppu21I YACGTR 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 20, 401
RsaI GTAC 2 cut(s) 442, 603
RsaNI GTAC 2 cut(s) 441, 602
RseI CAYNNNNRTG 1 cut(s) 458
SalI GTCGAC 1 cut(s) 57
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 5 cut(s) 199, 217, 220, 227, 243
Sau3AI GATC 3 cut(s) 79, 162, 508
SchI GAGTC 1 cut(s) 99
SduI GDGCHC 1 cut(s) 553
SetI ASST 8 cut(s) 64, 195, 247, 306, 316, 474, 507, 585
SfaNI GCATC 2 cut(s) 75, 125
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
SnaBI TACGTA 1 cut(s) 314
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 6 cut(s) 71, 128, 336, 517, 543, 607
SsiI CCGC 4 cut(s) 51, 214, 217, 220
SspMI CTAG 4 cut(s) 41, 183, 405, 411
TaaI ACNGT 2 cut(s) 23, 457
TaiI ACGT 1 cut(s) 316
TaqI TCGA 5 cut(s) 58, 69, 75, 258, 507
TasI AATT 6 cut(s) 71, 128, 336, 517, 543, 607
TauI GCSGC 2 cut(s) 219, 222
TfiI GAWTC 2 cut(s) 375, 592
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 198, 226, 242
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 584, 584
TspGWI ACGGA 1 cut(s) 294
XapI RAATTY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 58
XspI CTAG 4 cut(s) 41, 183, 405, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.