Rroxscaffold_4G00320330

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004213
Physical Location & Seq
Reverse (-)
48956914 .. 48957576
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320330.1

Sequence Viewer

Length: 663 bp
ATGATGCTGTGCAAGAAGTTATGGTCAGACAGATTTAGTAATCTTCCGGATGAACTTGCTCATCATATTCTTTCCTTCCTCGGTGTCAGAGATCTCGCTTCTGTAGGGGCTGTGTCCAAAAGGTGTAGAGAAGTTTACCTCTCAGTTCCATCAGTGAATATTGATGCAAGTTGTTATCTCCCAGTTCCATTAGTGAATCTTGATGCAAGTTGTTATCTAAAAACCAGCAGCAAGTTAGGAGCGCTGATGTGTTTCTGGGATAGATACATGTTTCAGCGTGGTGTTAATAAGATGCAACGCTTTTACATTAATTGGACTTTCTTTACACGCGGCATTGCGAATAAAGACTCTGTGGAGCAATATCGAATAAACTCTTGGATTCATAACGCAGTAAGGTGTAATGTTGAAGAGCTTAACCTTATTTTCACATCATGTGGTGGATTATCGTTTGTGCTGCCATCTTGCCTATATCAGTCACAATCTTTCAAGTCTCTAGTATTAAAATGCTTGGATAAGATGACTCTTGAAGCACCACCAGTATTTCTTTCCTGCAATCTCCGTTACTTGCAGTTGACTTCTGTTAGAATAGCAGATGAAAAGTTTTGCAAGTGGATCTCCTCTAGCTGCAAATACATTAAGAAATTACAGCTTATTCAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD

Protein Analysis

220

Amino Acids

25.33

Weight (kDa)

9.15

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 12 - 45 5.7e-08 F-box-like
F-box PF00646 12 - 44 5.7e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 111 - 217 5.6e-09 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 124 - 218 9.4e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 330
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 1 cut(s) 330
AclWI GGATC 1 cut(s) 620
AfeI AGCGCT 1 cut(s) 243
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 4 cut(s) 407, 487, 527, 656
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
AluBI AGCT 3 cut(s) 412, 624, 649
AluI AGCT 3 cut(s) 412, 624, 649
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 46
Aor51HI AGCGCT 1 cut(s) 243
ApeKI GCWGC 3 cut(s) 228, 454, 624
AseI ATTAAT 1 cut(s) 309
AspLEI GCGC 1 cut(s) 244
BbvI GCAGC 3 cut(s) 240, 441, 611
BccI CCATC 2 cut(s) 157, 466
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 2 cut(s) 494, 621
BfmI CTRYAG 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 245
BglII AGATCT 1 cut(s) 91
BisI GCNGC 4 cut(s) 229, 331, 455, 625
BlsI GCNGC 4 cut(s) 230, 332, 456, 626
BmrI ACTGGG 1 cut(s) 176
BmsI GCATC 3 cut(s) 154, 193, 282
BmuI ACTGGG 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 79
BsaWI WCCGGW 1 cut(s) 46
Bse1I ACTGG 2 cut(s) 182, 536
Bse3DI GCAATG 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 46
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 182, 536
BseRI GAGGAG 1 cut(s) 607
BseXI GCAGC 3 cut(s) 240, 441, 611
Bsh1236I CGCG 1 cut(s) 330
BsiSI CCGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 495
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 2 cut(s) 91, 612
BspACI CCGC 1 cut(s) 330
BspCNI CTCAG 1 cut(s) 155
BspEI TCCGGA 1 cut(s) 46
BspFNI CGCG 1 cut(s) 330
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 333
BsrI ACTGG 2 cut(s) 182, 536
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 2 cut(s) 91, 612
Bst6I CTCTTC 1 cut(s) 402
BstDEI CTNAG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 330
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 2 cut(s) 94, 615
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 2 cut(s) 91, 612
BstNSI RCATGY 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 102
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 240, 441, 611
BstX2I RGATCY 2 cut(s) 91, 612
BstYI RGATCY 2 cut(s) 91, 612
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 159
CfoI GCGC 1 cut(s) 244
CviAII CATG 2 cut(s) 268, 432
CviJI RGCY 4 cut(s) 110, 412, 624, 649
CviKI_1 RGCY 4 cut(s) 110, 412, 624, 649
DdeI CTNAG 1 cut(s) 142
DpnI GATC 2 cut(s) 93, 614
DpnII GATC 2 cut(s) 91, 612
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco47III AGCGCT 1 cut(s) 243
FaeI CATG 2 cut(s) 271, 435
FaiI YATR 6 cut(s) 22, 66, 269, 384, 433, 469
FatI CATG 2 cut(s) 267, 431
Fnu4HI GCNGC 4 cut(s) 229, 331, 455, 625
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 4 cut(s) 229, 331, 455, 625
FspBI CTAG 2 cut(s) 494, 621
GlaI GCGC 1 cut(s) 243
GluI GCNGC 4 cut(s) 229, 331, 455, 625
HaeII RGCGCY 1 cut(s) 245
HapII CCGG 1 cut(s) 47
HhaI GCGC 1 cut(s) 244
Hin1II CATG 2 cut(s) 271, 435
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HinfI GANTC 4 cut(s) 196, 347, 379, 520
HpaII CCGG 1 cut(s) 47
Hpy166II GTNNAC 2 cut(s) 136, 573
Hpy188I TCNGA 2 cut(s) 28, 89
Hpy188III TCNNGA 3 cut(s) 47, 200, 524
Hpy8I GTNNAC 2 cut(s) 136, 573
HpyAV CCTTC 1 cut(s) 85
HpyCH4V TGCA 8 cut(s) 12, 167, 206, 295, 552, 568, 606, 627
HpyF3I CTNAG 1 cut(s) 142
Hsp92II CATG 2 cut(s) 271, 435
HspAI GCGC 1 cut(s) 242
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 91, 612
LguI GCTCTTC 1 cut(s) 402
LmnI GCTCC 2 cut(s) 239, 355
LpnPI CCDG 6 cut(s) 60, 195, 238, 241, 549, 562
Lsp1109I GCAGC 3 cut(s) 240, 441, 611
LweI GCATC 3 cut(s) 154, 193, 282
MaeI CTAG 2 cut(s) 494, 621
MaeIII GTNAC 2 cut(s) 474, 560
MalI GATC 2 cut(s) 93, 614
MboI GATC 2 cut(s) 91, 612
MboII GAAGA 2 cut(s) 35, 419
MflI RGATCY 2 cut(s) 91, 612
MluCI AATT 2 cut(s) 310, 641
MlyI GAGTC 2 cut(s) 341, 514
MnlI CCTC 3 cut(s) 89, 149, 628
MroI TCCGGA 1 cut(s) 46
MseI TTAA 6 cut(s) 285, 309, 414, 500, 636, 661
MspI CCGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 330
NdeII GATC 2 cut(s) 91, 612
NlaIII CATG 2 cut(s) 271, 435
NmuCI GTSAC 1 cut(s) 474
NspI RCATGY 1 cut(s) 271
PciI ACATGT 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 196, 379
PkrI GCNGC 4 cut(s) 230, 332, 456, 626
PleI GAGTC 2 cut(s) 341, 514
PpsI GAGTC 2 cut(s) 341, 514
PscI ACATGT 1 cut(s) 267
PshBI ATTAAT 1 cut(s) 309
PsuI RGATCY 2 cut(s) 91, 612
SapI GCTCTTC 1 cut(s) 402
SaqAI TTAA 6 cut(s) 285, 309, 414, 500, 636, 661
SatI GCNGC 4 cut(s) 229, 331, 455, 625
Sau3AI GATC 2 cut(s) 91, 612
SchI GAGTC 2 cut(s) 341, 514
SetI ASST 7 cut(s) 125, 141, 398, 414, 420, 626, 651
SfaNI GCATC 3 cut(s) 154, 193, 282
SfcI CTRYAG 1 cut(s) 102
Sse9I AATT 2 cut(s) 310, 641
SsiI CCGC 1 cut(s) 330
SspI AATATT 1 cut(s) 160
SspMI CTAG 2 cut(s) 494, 621
TaqI TCGA 1 cut(s) 364
TasI AATT 2 cut(s) 310, 641
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 2 cut(s) 196, 379
Tru1I TTAA 6 cut(s) 285, 309, 414, 500, 636, 661
Tru9I TTAA 6 cut(s) 285, 309, 414, 500, 636, 661
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 1 cut(s) 474
TseI GCWGC 3 cut(s) 228, 454, 624
Tsp45I GTSAC 1 cut(s) 474
TspDTI ATGAA 3 cut(s) 66, 371, 609
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 1 cut(s) 159
VspI ATTAAT 1 cut(s) 309
XceI RCATGY 1 cut(s) 271
XspI CTAG 2 cut(s) 494, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.