MD04G1145900.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004213
Physical Location & Seq
Forward (+)
23456693 .. 23459355
2663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1145900.v1.1.491

Sequence Viewer

Length: 423 bp
ATGCCTAATCTGAACATTTTCTGCATAAAGACTAGATGTCACAGATTGACTCCTAGAACAGCAAGTCGCTTTGGTAATGAGTACTGGAAACTGCAAAACCTTGCTTTTATTCACCAGCTTAAGGAGGTAAGCATAGAGAACTCCTATGGGTCTAATGAAATCGAGTTTGCAAGGTATATTCTCGAGCATGCTCAAAATTTGAAGAAAATGGTCATTGTTCATCGTGATCAAAATGCTCCATCAAAAGTGGCTGGGATGATGACTAGGAGCAAGATGATATCCGCTGCTTCAGTTCTCATTCGGCAGAACCCAAAGCACTTCTACACTATCACTTATTATGGTCCTGGGATCTCACATTGCGATTTTGATATCTCAGATAGTGATTCGAAGAGCTCAGATAGAGATTCTGACTTTCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

141

Amino Acids

16.15

Weight (kDa)

9.03

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 40 - 72 5.4e-07 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 282
AclWI GGATC 1 cut(s) 356
AcsI RAATTY 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 121
AflII CTTAAG 1 cut(s) 119
AgsI TTSAA 1 cut(s) 202
AhdI GACNNNNNGTC 1 cut(s) 36
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 2 cut(s) 118, 393
AluI AGCT 2 cut(s) 118, 393
Alw21I GWGCWC 1 cut(s) 395
AlwI GGATC 1 cut(s) 356
Ama87I CYCGRG 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 104
AsuII TTCGAA 1 cut(s) 386
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 1 cut(s) 271
BccI CCATC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 345
BclI TGATCA 1 cut(s) 226
BfaI CTAG 3 cut(s) 33, 54, 264
BfrI CTTAAG 1 cut(s) 119
BisI GCNGC 1 cut(s) 285
BlsI GCNGC 1 cut(s) 286
BmcAI AGTACT 1 cut(s) 83
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 341
BmeRI GACNNNNNGTC 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 341
BmrFI CCNGG 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 2 cut(s) 387, 408
BseNI ACTGG 1 cut(s) 89
BseXI GCAGC 1 cut(s) 271
BseYI CCCAGC 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 226, 348
BspACI CCGC 1 cut(s) 282
BspCNI CTCAG 2 cut(s) 386, 407
BspPI GGATC 1 cut(s) 356
BspQI GCTCTTC 1 cut(s) 383
BspT104I TTCGAA 1 cut(s) 386
BspTI CTTAAG 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 355
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 2 cut(s) 226, 348
Bst2UI CCWGG 1 cut(s) 345
Bst6I CTCTTC 1 cut(s) 383
BstAFI CTTAAG 1 cut(s) 119
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 2 cut(s) 373, 394
BstF5I GGATG 1 cut(s) 261
BstKTI GATC 2 cut(s) 229, 351
BstMBI GATC 2 cut(s) 226, 348
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 271
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BtsCI GGATG 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 82
CviAII CATG 1 cut(s) 188
CviJI RGCY 3 cut(s) 118, 251, 393
CviKI_1 RGCY 3 cut(s) 118, 251, 393
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 2 cut(s) 373, 394
DpnI GATC 2 cut(s) 228, 350
DpnII GATC 2 cut(s) 226, 348
DriI GACNNNNNGTC 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 383
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 383
Ecl136II GAGCTC 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 395
Eco32I GATATC 2 cut(s) 279, 370
Eco47I GGWCC 1 cut(s) 341
Eco53kI GAGCTC 1 cut(s) 393
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 343
EcoRV GATATC 2 cut(s) 279, 370
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 1 cut(s) 191
FaiI YATR 6 cut(s) 26, 134, 147, 177, 189, 339
FatI CATG 1 cut(s) 187
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 1 cut(s) 285
FokI GGATG 1 cut(s) 268
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 285
FspBI CTAG 3 cut(s) 33, 54, 264
GluI GCNGC 1 cut(s) 285
GsaI CCCAGC 1 cut(s) 255
Hin1II CATG 1 cut(s) 191
HinfI GANTC 3 cut(s) 49, 383, 404
HphI GGTGA 1 cut(s) 104
Hpy188I TCNGA 5 cut(s) 12, 376, 397, 409, 417
Hpy188III TCNNGA 2 cut(s) 182, 224
HpyCH4V TGCA 3 cut(s) 24, 94, 170
HpyF3I CTNAG 2 cut(s) 373, 394
Hsp92II CATG 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 2 cut(s) 226, 348
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 2 cut(s) 241, 267
LpnPI CCDG 5 cut(s) 70, 128, 237, 330, 357
Lsp1109I GCAGC 1 cut(s) 271
MaeI CTAG 3 cut(s) 33, 54, 264
MaeIII GTNAC 1 cut(s) 38
MalI GATC 2 cut(s) 228, 350
MboI GATC 2 cut(s) 226, 348
MboII GAAGA 2 cut(s) 214, 400
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 1 cut(s) 196
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 1 cut(s) 118
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 120, 421
MspA1I CMGCKG 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
NdeII GATC 2 cut(s) 226, 348
NlaIII CATG 1 cut(s) 191
NmuCI GTSAC 1 cut(s) 38
NspI RCATGY 1 cut(s) 191
NspV TTCGAA 1 cut(s) 386
PaeI GCATGC 1 cut(s) 191
PaeR7I CTCGAG 1 cut(s) 182
PciSI GCTCTTC 1 cut(s) 383
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 383, 404
PkrI GCNGC 1 cut(s) 286
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 343
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 1 cut(s) 343
PspPI GGNCC 1 cut(s) 341
PsuI RGATCY 1 cut(s) 348
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SacI GAGCTC 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 2 cut(s) 120, 421
SatI GCNGC 1 cut(s) 285
Sau3AI GATC 2 cut(s) 226, 348
Sau96I GGNCC 1 cut(s) 341
ScaI AGTACT 1 cut(s) 83
SchI GAGTC 1 cut(s) 43
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 395
SetI ASST 5 cut(s) 102, 120, 129, 176, 395
Sfr274I CTCGAG 1 cut(s) 182
SfuI TTCGAA 1 cut(s) 386
SinI GGWCC 1 cut(s) 341
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 119, 182
SmoI CTYRAG 2 cut(s) 119, 182
SphI GCATGC 1 cut(s) 191
Sse9I AATT 1 cut(s) 196
SsiI CCGC 1 cut(s) 282
SspMI CTAG 3 cut(s) 33, 54, 264
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 343
TaqI TCGA 3 cut(s) 162, 183, 386
TasI AATT 1 cut(s) 196
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 2 cut(s) 383, 404
Tru1I TTAA 2 cut(s) 120, 421
Tru9I TTAA 2 cut(s) 120, 421
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 1 cut(s) 284
Tsp45I GTSAC 1 cut(s) 38
TspDTI ATGAA 2 cut(s) 171, 209
Vha464I CTTAAG 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 1 cut(s) 196
XceI RCATGY 1 cut(s) 191
XhoI CTCGAG 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 3 cut(s) 33, 54, 264
ZrmI AGTACT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.