Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004213

Physical Location & Seq

Genomic Coordinates Forward (+)

23422199 .. 23426243

Gene Structure 4045 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1145500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGCGCGCAAGCGTAAGCTCCAGGCACCTACAAGTTGTGAATCTGAAGGAACTTGTTCTGAAAGTGGAATGGACCGATTTAGTGATCTTCCGGAGGAAGTTGTTCATCGGATTCTTTCCTTCCTCAATTTCAAAGACCTCACTCGAGTGGGTGCTGTATCCAAAAGATGCAGACAAGTTCGTCTCTCAGTTCCGGTGGTGAATTTTGGTACAGATGAGCGGACAGGCATAAAAAAAATGGATTTAGGGAGGATGATGACTTCTTTTGATCGGTACTTGCTCGATCGTGGTCCTAACAGGTTGCAGCGAGTCTGCATCTGCTTGAGTGTCTGTAAAAGTGAAACAACGAAGAAAGTTTCTGATGACCATTCTCGAGTGCTCAAGTGGATTCGTAATGCAGTAAGGTGTAATGTTGAAGAGCTTGATCTCTATTTCTCACACCGTGTTACTAGTACGTTCTCGTTGCCATCTTTCATCTTCCATTCTCAATCTTTGCGCTCCTTATCGGTGTCCTCAGGTGTAAGCATTTTGGAGGTTGTTGAAGCGCCGTCTCTATCCTTTTCTAGTAATCTCCATTACTTATCATTGAGTTCTGTTAAAATAGTGGACGACATGGTTTTCAAATGGATCTCATGCTGCTGCAAGTGCCTTAAGGAATTACGTCTTGTTTCTATCAATGGTATACCAAATATCTCCATTGAAAGTTCGTCGTTGGTTAACTTTTATGTTTTCGATTGTGGTGACGTACATCTTATTATATCGGGTCAGAAACTTGAATATATAGATATGGAGTGGGGTGGTTACGATGCATTTACGAGCAGAAAAAGCACAATTAGCAACACATCATTGAAGATTTTTGCTCCAAATCTTAAAACTTTGAAATGGAAAGGCGAATTGAGGAATAGCCAAAGTCTGGGAAATTTAAAGAGTTTGGAAGAAGTTGAGATTTTCTTAGCGCCTCTGAAAAATGAGTTCGACAAAGTATTGGAGGTTCTTTGCAGTATATGTTCGGCAAAAGTTATTACCATAAACGAGGAGACCATTAAGGCTCTGTACAAGGAAGGTTCCACGACAACACTAATATTGGATAATATTTTAAACTTGAGCATACATTGTGAGAACTTGAATAACAGGCTTGTTCCTGCAATGGTCTCTCTTCTTAGGAGAATGCCTAATCTCAATATTTTGTGCATAAAGACTAGCTGTGAGAATTTGACTCCTGAAAAAGCAAGTCTCTCCAGTAAGGAATGCTGGAAACTGCAAAACCTTGTTTTCATTCATCAGCTTAAGGAGGCAAGTCACTTTGGTAATGAATACTGGAAACTGCAAAACCTTGCTTTCATTCATCAGCTTAAGGAGGTAAGCATAGTGAATTCCTATGGATCTAATGAAATCGAGTTTGCAAGATATATTCTCGAGCGTGCTCAAAATTTGAAGAAAATGGTCATTGTTCATCGTGACGATGATGCTCCATCAAAAGTTGCTGGGATGGTGAGTAGGAGCAAGATGATTTCCAGTGCTTCCATTCTCATCCAGCAGGATCCACACAGGATTTACAGAATTTGTGGTCCGGGATCTTATATTTTGTATTCGGGACCTGATATATTGAAATCTGGGATAGTGACTATGAGCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBD

Protein Analysis

546

Amino Acids

61.56

Weight (kDa)

8.84

Isoelectric Point (pI)

42.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	27 - 59	5.5e-08	F-box domain
F-box-like	PF12937	27 - 59	1.3e-07	F-box-like
LRR_At5g56370	PF24758	128 - 259	2.9e-11	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004213)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g16170 FvH4_6g18953 FvH4_7g04740 FvH4_7g04740
malus_domestica	MD02G1269000.v1.1 MD04G1145300.v1.1 MD04G1145500.v1.1 MD04G1145600.v1.1 MD04G1145900.v1.1 MD12G1159100.v1.1
prunus_persica	Prupe.2G052100_v2.0.a1 Prupe.7G105700_v2.0.a1 Prupe.7G106400_v2.0.a1
pyrus_communis	pycom04g13150
rosa_chinensis	RchiOBHm_Chr1g0330111
rosa_laevigata	RLG00000029830
rosa_multiflora	Rmu_sc0004673.1_g000009
rosa_roxburghii	Rroxscaffold_4G00320200 Rroxscaffold_4G00320300 Rroxscaffold_4G00320320 Rroxscaffold_4G00320330
rosa_rugosa	Rorug01G0086000 Rorug01G0086100 Rorug01G0086200 Rorug02G0131100 Rorug03G0137300
rosa_samantha	Rh1AG104600 Rh1AG104700 Rh1BG082800 Rh1CG100200 Rh1DG107500 Rh1DG108500
rosa_wichuraiana	Rw0G015890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	180
AccB1I	GGYRCC	1 cut(s)	25
AccB7I	CCANNNNNTGG	1 cut(s)	913
AccBSI	CCGCTC	1 cut(s)	220
AccI	GTMKAC	1 cut(s)	682
AccII	CGCG	1 cut(s)	7
AccIII	TCCGGA	1 cut(s)	91
AciI	CCGC	1 cut(s)	220
AclWI	GGATC	5 cut(s)	635, 1390, 1535, 1548, 1582
AcsI	RAATTY	6 cut(s)	202, 919, 1210, 1372, 1429, 1560
AcuI	CTGAAG	1 cut(s)	66
AdeI	CACNNNGTG	1 cut(s)	443
AfaI	GTAC	5 cut(s)	211, 275, 454, 747, 1055
AfiI	CCNNNNNNNGG	1 cut(s)	913
AflII	CTTAAG	3 cut(s)	650, 1286, 1352
AhlI	ACTAGT	1 cut(s)	449
AjnI	CCWGG	1 cut(s)	21
AleI	CACNNNNGTG	1 cut(s)	146
AluBI	AGCT	6 cut(s)	19, 421, 1203, 1285, 1351, 1632
AluI	AGCT	6 cut(s)	19, 421, 1203, 1285, 1351, 1632
Alw21I	GWGCWC	3 cut(s)	381, 1426, 1634
Alw26I	GTCTC	5 cut(s)	188, 555, 1031, 1156, 1238
AlwI	GGATC	5 cut(s)	635, 1390, 1535, 1548, 1582
Ama87I	CYCGRG	3 cut(s)	144, 372, 1415
Aor13HI	TCCGGA	1 cut(s)	91
ApeKI	GCWGC	3 cut(s)	304, 636, 639
ApoI	RAATTY	6 cut(s)	202, 919, 1210, 1372, 1429, 1560
Asp700I	GAANNNNTTC	2 cut(s)	55, 102
AspLEI	GCGC	5 cut(s)	7, 9, 498, 547, 958
AspS9I	GGNCC	4 cut(s)	73, 290, 1568, 1595
AsuC2I	CCSGG	1 cut(s)	1572
AsuHPI	GGTGA	3 cut(s)	211, 752, 1504
AvaI	CYCGRG	3 cut(s)	144, 372, 1415
AvaII	GGWCC	4 cut(s)	73, 290, 1568, 1595
AxyI	CCTNAGG	1 cut(s)	514
BamHI	GGATCC	1 cut(s)	1540
BanI	GGYRCC	1 cut(s)	25
BanII	GRGCYC	1 cut(s)	1634
Bbv12I	GWGCWC	3 cut(s)	381, 1426, 1634
BbvI	GCAGC	3 cut(s)	316, 623, 626
BccI	CCATC	3 cut(s)	475, 1480, 1483
BceAI	ACGGC	1 cut(s)	532
BciT130I	CCWGG	1 cut(s)	23
BciVI	GTATCC	1 cut(s)	169
BcnI	CCSGG	1 cut(s)	1572
BcoDI	GTCTC	5 cut(s)	188, 555, 1031, 1156, 1238
BcuI	ACTAGT	1 cut(s)	449
BfaI	CTAG	3 cut(s)	450, 564, 1200
BfoI	RGCGCY	2 cut(s)	548, 959
BfrI	CTTAAG	3 cut(s)	650, 1286, 1352
BfuI	GTATCC	1 cut(s)	169
BisI	GCNGC	3 cut(s)	305, 637, 640
BlsI	GCNGC	3 cut(s)	306, 638, 641
Bme1390I	CCNGG	2 cut(s)	23, 1572
Bme18I	GGWCC	4 cut(s)	73, 290, 1568, 1595
BmeT110I	CYCGRG	3 cut(s)	144, 372, 1415
BmgT120I	GGNCC	4 cut(s)	73, 290, 1568, 1595
BmiI	GGNNCC	4 cut(s)	27, 1066, 1542, 1596
BmrFI	CCNGG	2 cut(s)	23, 1572
BmsI	GCATC	4 cut(s)	158, 324, 796, 1456
BpmI	CTGGAG	2 cut(s)	5, 1222
BpuEI	CTTGAG	3 cut(s)	343, 365, 1123
BpuMI	CCSGG	1 cut(s)	1572
BsaI	GGTCTC	2 cut(s)	1031, 1156
BsaWI	WCCGGW	2 cut(s)	91, 193
Bsc4I	CCNNNNNNNGG	1 cut(s)	913
Bse1I	ACTGG	3 cut(s)	1239, 1322, 1515
Bse21I	CCTNAGG	1 cut(s)	514
Bse3DI	GCAATG	1 cut(s)	1152
BseAI	TCCGGA	1 cut(s)	91
BseBI	CCWGG	1 cut(s)	23
BseGI	GGATG	3 cut(s)	258, 1494, 1530
BseLI	CCNNNNNNNGG	1 cut(s)	913
BseMI	GCAATG	1 cut(s)	1152
BseMII	CTCAG	2 cut(s)	201, 528
BseNI	ACTGG	3 cut(s)	1239, 1322, 1515
BsePI	GCGCGC	1 cut(s)	5
BseRI	GAGGAG	1 cut(s)	1049
BseXI	GCAGC	3 cut(s)	316, 623, 626
BseYI	CCCAGC	1 cut(s)	1484
Bsh1236I	CGCG	1 cut(s)	7
Bsh1285I	CGRYCG	1 cut(s)	286
BshNI	GGYRCC	1 cut(s)	25
BsiEI	CGRYCG	1 cut(s)	286
BsiHKAI	GWGCWC	3 cut(s)	381, 1426, 1634
BsiHKCI	CYCGRG	3 cut(s)	144, 372, 1415
BsiSI	CCGG	3 cut(s)	92, 194, 1571
BslFI	GGGAC	1 cut(s)	1608
BslI	CCNNNNNNNGG	1 cut(s)	913
BsmAI	GTCTC	5 cut(s)	188, 555, 1031, 1156, 1238
BsmBI	CGTCTC	2 cut(s)	188, 555
BsmFI	GGGAC	1 cut(s)	1608
BsmI	GAATGC	2 cut(s)	1173, 1253
Bso31I	GGTCTC	2 cut(s)	1031, 1156
BsoBI	CYCGRG	3 cut(s)	144, 372, 1415
Bsp1286I	GDGCHC	3 cut(s)	381, 1426, 1634
Bsp13I	TCCGGA	1 cut(s)	91
Bsp1407I	TGTACA	1 cut(s)	1053
Bsp143I	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
BspACI	CCGC	1 cut(s)	220
BspCNI	CTCAG	2 cut(s)	200, 527
BspEI	TCCGGA	1 cut(s)	91
BspFNI	CGCG	1 cut(s)	7
BspLI	GGNNCC	4 cut(s)	27, 1066, 1542, 1596
BspPI	GGATC	5 cut(s)	635, 1390, 1535, 1548, 1582
BspQI	GCTCTTC	1 cut(s)	411
BspT107I	GGYRCC	1 cut(s)	25
BspTI	CTTAAG	3 cut(s)	650, 1286, 1352
BspTNI	GGTCTC	2 cut(s)	1031, 1156
BsrBI	CCGCTC	1 cut(s)	220
BsrDI	GCAATG	1 cut(s)	1152
BsrGI	TGTACA	1 cut(s)	1053
BsrI	ACTGG	3 cut(s)	1239, 1322, 1515
BssHII	GCGCGC	1 cut(s)	5
BssMI	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
BssNAI	GTATAC	1 cut(s)	683
Bst1107I	GTATAC	1 cut(s)	683
Bst2UI	CCWGG	1 cut(s)	23
Bst4CI	ACNGT	1 cut(s)	443
Bst6I	CTCTTC	2 cut(s)	411, 1161
BstAFI	CTTAAG	3 cut(s)	650, 1286, 1352
BstAUI	TGTACA	1 cut(s)	1053
BstC8I	GCNNGC	3 cut(s)	7, 11, 1422
BstDEI	CTNAG	5 cut(s)	187, 514, 952, 1160, 1635
BstF5I	GGATG	3 cut(s)	258, 1494, 1530
BstFNI	CGCG	1 cut(s)	7
BstH2I	RGCGCY	2 cut(s)	548, 959
BstHHI	GCGC	5 cut(s)	7, 9, 498, 547, 958
BstKTI	GATC	8 cut(s)	88, 271, 286, 427, 630, 1385, 1543, 1577
BstMAI	GTCTC	5 cut(s)	188, 555, 1031, 1156, 1238
BstMBI	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
BstMCI	CGRYCG	1 cut(s)	286
BstMWI	GCNNNNNNNGC	3 cut(s)	645, 825, 834
BstNI	CCWGG	1 cut(s)	23
BstSCI	CCNGG	2 cut(s)	21, 1570
BstUI	CGCG	1 cut(s)	7
BstV1I	GCAGC	3 cut(s)	316, 623, 626
BstX2I	RGATCY	4 cut(s)	627, 1382, 1540, 1574
BstYI	RGATCY	4 cut(s)	627, 1382, 1540, 1574
BstZ17I	GTATAC	1 cut(s)	683
Bsu36I	CCTNAGG	1 cut(s)	514
BsuI	GTATCC	1 cut(s)	169
BtsCI	GGATG	3 cut(s)	258, 1494, 1530
BtsIMutI	CAGTG	1 cut(s)	1522
Cac8I	GCNNGC	3 cut(s)	7, 11, 1422
CfoI	GCGC	5 cut(s)	7, 9, 498, 547, 958
Cfr13I	GGNCC	4 cut(s)	73, 290, 1568, 1595
Csp6I	GTAC	5 cut(s)	210, 274, 453, 746, 1054
CviAII	CATG	2 cut(s)	613, 633
CviJI	RGCY	9 cut(s)	19, 421, 906, 1049, 1135, 1203, 1285, 1351, 1632
CviKI_1	RGCY	9 cut(s)	19, 421, 906, 1049, 1135, 1203, 1285, 1351, 1632
CviQI	GTAC	5 cut(s)	210, 274, 453, 746, 1054
DdeI	CTNAG	5 cut(s)	187, 514, 952, 1160, 1635
DpnI	GATC	8 cut(s)	87, 270, 285, 426, 629, 1384, 1542, 1576
DpnII	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
DraI	TTTAAA	2 cut(s)	924, 1098
DraIII	CACNNNGTG	1 cut(s)	443
DrdI	GACNNNNNNGTC	1 cut(s)	180
DseDI	GACNNNNNNGTC	1 cut(s)	180
Eam1104I	CTCTTC	2 cut(s)	411, 1161
EarI	CTCTTC	2 cut(s)	411, 1161
Ecl136II	GAGCTC	1 cut(s)	1632
Eco24I	GRGCYC	1 cut(s)	1634
Eco31I	GGTCTC	2 cut(s)	1031, 1156
Eco47I	GGWCC	4 cut(s)	73, 290, 1568, 1595
Eco53kI	GAGCTC	1 cut(s)	1632
Eco57I	CTGAAG	1 cut(s)	66
Eco81I	CCTNAGG	1 cut(s)	514
Eco88I	CYCGRG	3 cut(s)	144, 372, 1415
EcoICRI	GAGCTC	1 cut(s)	1632
EcoO109I	RGGNCCY	1 cut(s)	1595
EcoRI	GAATTC	1 cut(s)	1372
EcoRII	CCWGG	1 cut(s)	21
EcoT22I	ATGCAT	1 cut(s)	811
EcoT38I	GRGCYC	1 cut(s)	1634
Esp3I	CGTCTC	2 cut(s)	188, 555
FaeI	CATG	2 cut(s)	616, 636
FaqI	GGGAC	1 cut(s)	1608
FatI	CATG	2 cut(s)	612, 632
FblI	GTMKAC	1 cut(s)	682
Fnu4HI	GCNGC	3 cut(s)	305, 637, 640
FokI	GGATG	3 cut(s)	265, 1501, 1517
FriOI	GRGCYC	1 cut(s)	1634
Fsp4HI	GCNGC	3 cut(s)	305, 637, 640
FspBI	CTAG	3 cut(s)	450, 564, 1200
GlaI	GCGC	5 cut(s)	6, 8, 497, 546, 957
GluI	GCNGC	3 cut(s)	305, 637, 640
GsaI	CCCAGC	1 cut(s)	1488
GsuI	CTGGAG	2 cut(s)	5, 1222
HaeII	RGCGCY	2 cut(s)	548, 959
HapII	CCGG	3 cut(s)	92, 194, 1571
HhaI	GCGC	5 cut(s)	7, 9, 498, 547, 958
Hin1II	CATG	2 cut(s)	616, 636
Hin6I	GCGC	5 cut(s)	5, 7, 496, 545, 956
HinP1I	GCGC	5 cut(s)	5, 7, 496, 545, 956
HincII	GTYRAC	1 cut(s)	718
HindII	GTYRAC	1 cut(s)	718
HinfI	GANTC	5 cut(s)	41, 112, 309, 388, 1216
HpaI	GTTAAC	1 cut(s)	718
HpaII	CCGG	3 cut(s)	92, 194, 1571
HphI	GGTGA	3 cut(s)	211, 752, 1504
Hpy166II	GTNNAC	3 cut(s)	607, 683, 718
Hpy188I	TCNGA	6 cut(s)	46, 61, 111, 361, 768, 963
Hpy188III	TCNNGA	6 cut(s)	92, 372, 1220, 1415, 1457, 1593
Hpy8I	GTNNAC	3 cut(s)	607, 683, 718
Hpy99I	CGWCG	1 cut(s)	712
HpyAV	CCTTC	3 cut(s)	41, 130, 1055
HpyCH4III	ACNGT	1 cut(s)	443
HpyCH4IV	ACGT	3 cut(s)	455, 661, 744
HpyF10VI	GCNNNNNNNGC	3 cut(s)	645, 825, 834
HpyF3I	CTNAG	5 cut(s)	187, 514, 952, 1160, 1635
HpySE526I	ACGT	3 cut(s)	455, 661, 744
Hsp92II	CATG	2 cut(s)	616, 636
HspAI	GCGC	5 cut(s)	5, 7, 496, 545, 956
Kpn2I	TCCGGA	1 cut(s)	91
KspAI	GTTAAC	1 cut(s)	718
Kzo9I	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
LguI	GCTCTTC	1 cut(s)	411
LmnI	GCTCC	5 cut(s)	24, 503, 865, 1474, 1500
Lsp1109I	GCAGC	3 cut(s)	316, 623, 626
LweI	GCATC	4 cut(s)	158, 324, 796, 1456
MaeI	CTAG	3 cut(s)	450, 564, 1200
MaeII	ACGT	3 cut(s)	455, 661, 744
MaeIII	GTNAC	6 cut(s)	445, 740, 800, 1298, 1457, 1621
MalI	GATC	8 cut(s)	87, 270, 285, 426, 629, 1384, 1542, 1576
MbiI	CCGCTC	1 cut(s)	220
MboI	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
MboII	GAAGA	8 cut(s)	80, 361, 428, 469, 862, 947, 1148, 1447
MflI	RGATCY	4 cut(s)	627, 1382, 1540, 1574
MhlI	GDGCHC	3 cut(s)	381, 1426, 1634
MlyI	GAGTC	2 cut(s)	318, 1210
Mph1103I	ATGCAT	1 cut(s)	811
MroI	TCCGGA	1 cut(s)	91
MroXI	GAANNNNTTC	2 cut(s)	55, 102
MseI	TTAA	9 cut(s)	597, 651, 717, 870, 923, 1044, 1097, 1287, 1353
MslI	CAYNNNNRTG	1 cut(s)	146
MspCI	CTTAAG	3 cut(s)	650, 1286, 1352
MspI	CCGG	3 cut(s)	92, 194, 1571
MspR9I	CCNGG	2 cut(s)	23, 1572
Mva1269I	GAATGC	2 cut(s)	1173, 1253
MvaI	CCWGG	1 cut(s)	23
MvnI	CGCG	1 cut(s)	7
MwoI	GCNNNNNNNGC	3 cut(s)	645, 825, 834
NciI	CCSGG	1 cut(s)	1572
NdeII	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
NlaIII	CATG	2 cut(s)	616, 636
NlaIV	GGNNCC	4 cut(s)	27, 1066, 1542, 1596
NmuCI	GTSAC	4 cut(s)	740, 1298, 1457, 1621
NsiI	ATGCAT	1 cut(s)	811
OliI	CACNNNNGTG	1 cut(s)	146
PaeR7I	CTCGAG	3 cut(s)	144, 372, 1415
PauI	GCGCGC	1 cut(s)	5
PciSI	GCTCTTC	1 cut(s)	411
PctI	GAATGC	2 cut(s)	1173, 1253
PdmI	GAANNNNTTC	2 cut(s)	55, 102
PfeI	GAWTC	3 cut(s)	41, 112, 388
PflMI	CCANNNNNTGG	1 cut(s)	913
PfoI	TCCNGGA	1 cut(s)	1570
PkrI	GCNGC	3 cut(s)	306, 638, 641
Ple19I	CGATCG	1 cut(s)	286
PleI	GAGTC	2 cut(s)	317, 1210
PpsI	GAGTC	2 cut(s)	317, 1210
PpuMI	RGGWCCY	1 cut(s)	1595
Psp124BI	GAGCTC	1 cut(s)	1634
Psp5II	RGGWCCY	1 cut(s)	1595
Psp6I	CCWGG	1 cut(s)	21
PspFI	CCCAGC	1 cut(s)	1484
PspGI	CCWGG	1 cut(s)	21
PspN4I	GGNNCC	4 cut(s)	27, 1066, 1542, 1596
PspPI	GGNCC	4 cut(s)	73, 290, 1568, 1595
PspPPI	RGGWCCY	1 cut(s)	1595
PspXI	VCTCGAGB	1 cut(s)	144
PsrI	GAACNNNNNNTAC	2 cut(s)	975, 1007
PsuI	RGATCY	4 cut(s)	627, 1382, 1540, 1574
PteI	GCGCGC	1 cut(s)	5
PvuI	CGATCG	1 cut(s)	286
RsaI	GTAC	5 cut(s)	211, 275, 454, 747, 1055
RsaNI	GTAC	5 cut(s)	210, 274, 453, 746, 1054
RseI	CAYNNNNRTG	1 cut(s)	146
SacI	GAGCTC	1 cut(s)	1634
SapI	GCTCTTC	1 cut(s)	411
SaqAI	TTAA	9 cut(s)	597, 651, 717, 870, 923, 1044, 1097, 1287, 1353
SatI	GCNGC	3 cut(s)	305, 637, 640
Sau3AI	GATC	8 cut(s)	85, 268, 283, 424, 627, 1382, 1540, 1574
Sau96I	GGNCC	4 cut(s)	73, 290, 1568, 1595
SchI	GAGTC	2 cut(s)	318, 1210
ScrFI	CCNGG	2 cut(s)	23, 1572
SduI	GDGCHC	3 cut(s)	381, 1426, 1634
SfaNI	GCATC	4 cut(s)	158, 324, 796, 1456
Sfr274I	CTCGAG	3 cut(s)	144, 372, 1415
SinI	GGWCC	4 cut(s)	73, 290, 1568, 1595
SlaI	CTCGAG	3 cut(s)	144, 372, 1415
SmiMI	CAYNNNNRTG	1 cut(s)	146
SmlI	CTYRAG	9 cut(s)	144, 322, 372, 380, 650, 1102, 1286, 1352, 1415
SmoI	CTYRAG	9 cut(s)	144, 322, 372, 380, 650, 1102, 1286, 1352, 1415
SpeI	ACTAGT	1 cut(s)	449
SsiI	CCGC	1 cut(s)	220
SspI	AATATT	3 cut(s)	1083, 1093, 1183
SspMI	CTAG	3 cut(s)	450, 564, 1200
SstI	GAGCTC	1 cut(s)	1634
StyD4I	CCNGG	2 cut(s)	21, 1570
TaaI	ACNGT	1 cut(s)	443
TaiI	ACGT	3 cut(s)	458, 664, 747
TaqI	TCGA	7 cut(s)	145, 282, 373, 732, 975, 1395, 1416
TaqII	GACCGA	1 cut(s)	90
TatI	WGTACW	1 cut(s)	1053
TfiI	GAWTC	3 cut(s)	41, 112, 388
Tru1I	TTAA	9 cut(s)	597, 651, 717, 870, 923, 1044, 1097, 1287, 1353
Tru9I	TTAA	9 cut(s)	597, 651, 717, 870, 923, 1044, 1097, 1287, 1353
TscAI	CASTG	1 cut(s)	1522
TseFI	GTSAC	4 cut(s)	740, 1298, 1457, 1621
TseI	GCWGC	3 cut(s)	304, 636, 639
Tsp45I	GTSAC	4 cut(s)	740, 1298, 1457, 1621
TspDTI	ATGAA	9 cut(s)	95, 463, 1264, 1268, 1326, 1330, 1334, 1404, 1442
TspRI	CASTG	1 cut(s)	1522
Van91I	CCANNNNNTGG	1 cut(s)	913
Vha464I	CTTAAG	3 cut(s)	650, 1286, 1352
VpaK11BI	GGWCC	4 cut(s)	73, 290, 1568, 1595
XapI	RAATTY	6 cut(s)	202, 919, 1210, 1372, 1429, 1560
XhoI	CTCGAG	3 cut(s)	144, 372, 1415
XmiI	GTMKAC	1 cut(s)	682
XmnI	GAANNNNTTC	2 cut(s)	55, 102
XspI	CTAG	3 cut(s)	450, 564, 1200
Zsp2I	ATGCAT	1 cut(s)	811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.