MD04G1150800.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000913
Physical Location & Seq
Reverse (-)
23962805 .. 23966750
3946 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1150800.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGGTTTGTGGTTGCTTCTCTGTGCTGAAATGGCGTAAAGAAGAAAGAGCTTCCTCGTCTTCCTTCCAGGAGCAAGGGATTATCAACAACAACGTAAGGCTTTTCTCGTATAATTCTCTGAGATCAGCTACTAGGAACTTTCATCCATCGAGCAGGATAGGTGGAGGAGGTTATGGAGTTGTCTATAGGGGAGTTCTAAGAGATGATGCCCAAGTTGCGATCAAATGTCTTTCCGCAGAATCTAGACAAGGAGCAAATGAATTTTTGACAGAGATTAATGTGATATCGCGTATACGACATCCAAACCTTGTTGAGCTCCTTGGTTGCTGTGTGGAGGACAACCATCGGATATTGGTATATGAATATCTGGAGAACAACAGTCTTTCTAGATCTTTGCTTGGTTCGAGAAGTAAATATGTTTTTCTAGATTGGCCAACGAGAGTTTCGATTTGCCTTGGTACAGCTACCGGTCTTGCATTCCTTCACGAGGAAGCTGAACAGCAAATTGTCCACAGAGATATCAAGGCTAGCAATATACTTCTTGATGCAAATTTTCGTCCTAAAATTGGAGATTTTGGGCTGGCAAAACTTTTTCCTGATAATGTCACTCATCTTAGTACTAGAGTAGCTGGAACGGAGGGATATCTCGCCCCAGAATATGCGCTTTTGGGACAACTTACGAAGAAAGCAGATGTGTACAGCTTTGGGGTGCTCTTGCTTGAGATAATTAGTGGCAGAAGTAGTAGTAAGGTAGCCTTTGGGGAGCAACTGCTGGTTCTCGTCGAATGGGTATGGAAACTAAGAGAAGAAGAAAGGCTCCTGGAACTTGTTGATCCAGAATTGACTGCATATCCAGAGGCCGAGGTTATGCGCTTCATCAAGGTTGCGCTGTTTTGTACTCAAGCCACTGCGCAACAGAGACCAACAATGACGCAAGTTGTGGAAATGCTTTCCAAAGAGGTCCACCTAAACGAGAAGGCACTAACAAAACCAAGGCCGAGGATGCATGCACCTGGAAAATTTGGCGGTACCAGTTCATTGGAGGAGACATCGTCATCATCTCAGTCACGCAAGAAATCAACAGCAAACCCTGATATAACTTCCACCAGCTCGTTGATCTTTGACGATCCTGAAACACAAATACTTCCGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

385

Amino Acids

42.92

Weight (kDa)

7.62

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 48 - 315 1.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 51 - 317 4.3e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 912
Acc65I GGTACC 1 cut(s) 1028
AccB1I GGYRCC 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 292
AccII CGCG 1 cut(s) 289
AciI CCGC 2 cut(s) 234, 1026
AclWI GGATC 2 cut(s) 827, 1121
AcoI YGGCCR 1 cut(s) 431
AcsI RAATTY 3 cut(s) 260, 550, 1019
AfaI GTAC 5 cut(s) 460, 619, 698, 898, 1030
AfiI CCNNNNNNNGG 2 cut(s) 487, 566
AgeI ACCGGT 1 cut(s) 467
AjnI CCWGG 3 cut(s) 66, 819, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 427, 459
AluBI AGCT 8 cut(s) 50, 128, 316, 464, 494, 629, 702, 1110
AluI AGCT 8 cut(s) 50, 128, 316, 464, 494, 629, 702, 1110
Alw21I GWGCWC 2 cut(s) 318, 714
Alw26I GTCTC 2 cut(s) 913, 1040
AlwI GGATC 2 cut(s) 827, 1121
AoxI GGCC 3 cut(s) 431, 858, 995
ApoI RAATTY 3 cut(s) 260, 550, 1019
AseI ATTAAT 1 cut(s) 276
AsiGI ACCGGT 1 cut(s) 467
Asp718I GGTACC 1 cut(s) 1028
AspLEI GCGC 4 cut(s) 664, 873, 889, 913
AspS9I GGNCC 1 cut(s) 961
AsuNHI GCTAGC 1 cut(s) 527
AvaII GGWCC 1 cut(s) 961
BalI TGGCCA 1 cut(s) 433
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 318
BauI CACGAG 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 318, 714
BccI CCATC 2 cut(s) 154, 351
BciT130I CCWGG 3 cut(s) 68, 821, 1014
BcoDI GTCTC 2 cut(s) 913, 1040
BfaI CTAG 6 cut(s) 132, 243, 387, 425, 528, 621
BfmI CTRYAG 1 cut(s) 184
BglII AGATCT 1 cut(s) 389
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 3 cut(s) 68, 821, 1014
Bme18I GGWCC 1 cut(s) 961
BmgT120I GGNCC 1 cut(s) 961
BmiI GGNNCC 2 cut(s) 818, 1030
BmrFI CCNGG 3 cut(s) 68, 821, 1014
BmsI GCATC 3 cut(s) 196, 535, 993
BmtI GCTAGC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 1 cut(s) 389
BpuEI CTTGAG 2 cut(s) 740, 885
BsaI GGTCTC 1 cut(s) 913
BsaJI CCNNGG 5 cut(s) 319, 454, 861, 992, 998
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 1037, 1067
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 566
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 1032
BseBI CCWGG 3 cut(s) 68, 821, 1014
BseDI CCNNGG 5 cut(s) 319, 454, 861, 992, 998
BseGI GGATG 3 cut(s) 142, 298, 1008
BseLI CCNNNNNNNGG 2 cut(s) 487, 566
BseMII CTCAG 2 cut(s) 110, 1076
BseNI ACTGG 1 cut(s) 1032
BseRI GAGGAG 2 cut(s) 180, 1058
Bsh1236I CGCG 1 cut(s) 289
BshFI GGCC 3 cut(s) 433, 860, 997
BshNI GGYRCC 1 cut(s) 1028
BshTI ACCGGT 1 cut(s) 467
BsiHKAI GWGCWC 2 cut(s) 318, 714
BsiSI CCGG 1 cut(s) 468
BslFI GGGAC 1 cut(s) 684
BslI CCNNNNNNNGG 2 cut(s) 487, 566
BsmAI GTCTC 2 cut(s) 913, 1040
BsmFI GGGAC 1 cut(s) 684
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 3 cut(s) 433, 860, 997
Bso31I GGTCTC 1 cut(s) 913
Bsp1286I GDGCHC 2 cut(s) 318, 714
Bsp1407I TGTACA 1 cut(s) 696
Bsp143I GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
BspACI CCGC 2 cut(s) 234, 1026
BspANI GGCC 3 cut(s) 433, 860, 997
BspCNI CTCAG 2 cut(s) 111, 1075
BspFNI CGCG 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 818, 1030
BspOI GCTAGC 1 cut(s) 531
BspPI GGATC 2 cut(s) 827, 1121
BspT107I GGYRCC 1 cut(s) 1028
BspTNI GGTCTC 1 cut(s) 913
BsrFI RCCGGY 1 cut(s) 467
BsrGI TGTACA 1 cut(s) 696
BsrI ACTGG 1 cut(s) 1032
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 5 cut(s) 319, 454, 861, 992, 998
BssMI GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
BssNAI GTATAC 1 cut(s) 293
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 3 cut(s) 319, 454, 992
Bst1107I GTATAC 1 cut(s) 293
Bst2BI CACGAG 1 cut(s) 485
Bst2UI CCWGG 3 cut(s) 68, 821, 1014
Bst4CI ACNGT 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 696
BstC8I GCNNGC 3 cut(s) 529, 582, 1008
BstDEI CTNAG 5 cut(s) 119, 197, 614, 800, 1062
BstENI CCTNNNNNAGG 1 cut(s) 485
BstF5I GGATG 3 cut(s) 142, 298, 1008
BstFNI CGCG 1 cut(s) 289
BstHHI GCGC 4 cut(s) 664, 873, 889, 913
BstKTI GATC 6 cut(s) 125, 222, 392, 835, 1119, 1129
BstMAI GTCTC 2 cut(s) 913, 1040
BstMBI GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 31, 215, 1003
BstNI CCWGG 3 cut(s) 68, 821, 1014
BstNSI RCATGY 1 cut(s) 1010
BstSCI CCNGG 3 cut(s) 66, 819, 1012
BstSFI CTRYAG 1 cut(s) 184
BstUI CGCG 1 cut(s) 289
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 389
BstXI CCANNNNNNTGG 1 cut(s) 1039
BstYI RGATCY 1 cut(s) 389
BstZ17I GTATAC 1 cut(s) 293
BsuRI GGCC 3 cut(s) 433, 860, 997
BtsCI GGATG 3 cut(s) 142, 298, 1008
BtsI GCAGTG 1 cut(s) 906
BtsIMutI CAGTG 1 cut(s) 906
Cac8I GCNNGC 3 cut(s) 529, 582, 1008
CfoI GCGC 4 cut(s) 664, 873, 889, 913
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 961
CseI GACGC 1 cut(s) 940
Csp6I GTAC 5 cut(s) 459, 618, 697, 897, 1029
CspAI ACCGGT 1 cut(s) 467
CviAII CATG 1 cut(s) 1007
CviQI GTAC 5 cut(s) 459, 618, 697, 897, 1029
DdeI CTNAG 5 cut(s) 119, 197, 614, 800, 1062
DpnI GATC 6 cut(s) 124, 221, 391, 834, 1118, 1128
DpnII GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
EaeI YGGCCR 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 3 cut(s) 319, 454, 992
Eco24I GRGCYC 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 913
Eco32I GATATC 3 cut(s) 285, 520, 644
Eco47I GGWCC 1 cut(s) 961
Eco53kI GAGCTC 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 485
EcoRII CCWGG 3 cut(s) 66, 819, 1012
EcoRV GATATC 3 cut(s) 285, 520, 644
EcoT14I CCWWGG 3 cut(s) 319, 454, 992
EcoT22I ATGCAT 1 cut(s) 1008
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 3 cut(s) 319, 454, 992
FaeI CATG 1 cut(s) 1010
FalI AAGNNNNNCTT 2 cut(s) 740, 772
FaqI GGGAC 1 cut(s) 684
FatI CATG 1 cut(s) 1006
FblI GTMKAC 1 cut(s) 292
FokI GGATG 3 cut(s) 129, 285, 1015
FriOI GRGCYC 1 cut(s) 318
FspBI CTAG 6 cut(s) 132, 243, 387, 425, 528, 621
FspI TGCGCA 1 cut(s) 912
GlaI GCGC 4 cut(s) 663, 872, 888, 912
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 3 cut(s) 433, 860, 997
HapII CCGG 1 cut(s) 468
HgaI GACGC 1 cut(s) 940
HhaI GCGC 4 cut(s) 664, 873, 889, 913
Hin1II CATG 1 cut(s) 1010
Hin6I GCGC 4 cut(s) 662, 871, 887, 911
HinP1I GCGC 4 cut(s) 662, 871, 887, 911
HinfI GANTC 1 cut(s) 239
HpaII CCGG 1 cut(s) 468
Hpy166II GTNNAC 4 cut(s) 293, 511, 697, 964
Hpy188I TCNGA 3 cut(s) 120, 348, 1149
Hpy8I GTNNAC 4 cut(s) 293, 511, 697, 964
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 3 cut(s) 73, 491, 970
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 5 cut(s) 476, 548, 848, 1006, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 215, 1003
HpyF3I CTNAG 5 cut(s) 119, 197, 614, 800, 1062
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 1 cut(s) 1010
HspAI GCGC 4 cut(s) 662, 871, 887, 911
KpnI GGTACC 1 cut(s) 1032
Kzo9I GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
LmnI GCTCC 5 cut(s) 70, 251, 321, 763, 822
LweI GCATC 3 cut(s) 196, 535, 993
MaeI CTAG 6 cut(s) 132, 243, 387, 425, 528, 621
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 2 cut(s) 604, 1065
MalI GATC 6 cut(s) 124, 221, 391, 834, 1118, 1128
MboI GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
MboII GAAGA 5 cut(s) 51, 53, 694, 818, 821
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 2 cut(s) 318, 714
MlsI TGGCCA 1 cut(s) 433
MluCI AATT 8 cut(s) 112, 260, 504, 550, 564, 726, 839, 1019
MluNI TGGCCA 1 cut(s) 433
Mox20I TGGCCA 1 cut(s) 433
Mph1103I ATGCAT 1 cut(s) 1008
MscI TGGCCA 1 cut(s) 433
MseI TTAA 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 433
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 3 cut(s) 68, 821, 1014
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 3 cut(s) 68, 821, 1014
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 3 cut(s) 31, 215, 1003
NdeII GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
NheI GCTAGC 1 cut(s) 527
NlaIII CATG 1 cut(s) 1010
NlaIV GGNNCC 2 cut(s) 818, 1030
NmeAIII GCCGAG 2 cut(s) 886, 1023
NmuCI GTSAC 2 cut(s) 604, 1065
NsbI TGCGCA 1 cut(s) 912
NsiI ATGCAT 1 cut(s) 1008
NspI RCATGY 1 cut(s) 1010
PaeI GCATGC 1 cut(s) 1010
PcsI WCGNNNNNNNCGW 1 cut(s) 443
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 1 cut(s) 239
PflFI GACNNNGTC 1 cut(s) 1051
PfoI TCCNGGA 2 cut(s) 66, 819
PinAI ACCGGT 1 cut(s) 467
PshBI ATTAAT 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 3 cut(s) 66, 819, 1012
PspGI CCWGG 3 cut(s) 66, 819, 1012
PspN4I GGNNCC 2 cut(s) 818, 1030
PspPI GGNCC 1 cut(s) 961
PsuI RGATCY 1 cut(s) 389
PsyI GACNNNGTC 1 cut(s) 1051
RsaI GTAC 5 cut(s) 460, 619, 698, 898, 1030
RsaNI GTAC 5 cut(s) 459, 618, 697, 897, 1029
SacI GAGCTC 1 cut(s) 318
SaqAI TTAA 1 cut(s) 276
Sau3AI GATC 6 cut(s) 122, 219, 389, 832, 1116, 1126
Sau96I GGNCC 1 cut(s) 961
ScaI AGTACT 1 cut(s) 619
ScrFI CCNGG 3 cut(s) 68, 821, 1014
SduI GDGCHC 2 cut(s) 318, 714
SfaNI GCATC 3 cut(s) 196, 535, 993
SfcI CTRYAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 961
SmlI CTYRAG 2 cut(s) 719, 900
SmoI CTYRAG 2 cut(s) 719, 900
SphI GCATGC 1 cut(s) 1010
Sse9I AATT 8 cut(s) 112, 260, 504, 550, 564, 726, 839, 1019
SsiI CCGC 2 cut(s) 234, 1026
SspMI CTAG 6 cut(s) 132, 243, 387, 425, 528, 621
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 3 cut(s) 66, 819, 1012
StyI CCWWGG 3 cut(s) 319, 454, 992
TaaI ACNGT 1 cut(s) 380
TaiI ACGT 1 cut(s) 96
TaqI TCGA 4 cut(s) 149, 404, 446, 783
TasI AATT 8 cut(s) 112, 260, 504, 550, 564, 726, 839, 1019
TatI WGTACW 3 cut(s) 617, 696, 896
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 1 cut(s) 913
TseFI GTSAC 2 cut(s) 604, 1065
Tsp45I GTSAC 2 cut(s) 604, 1065
TspDTI ATGAA 5 cut(s) 131, 273, 375, 865, 1026
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 1 cut(s) 913
Tth111I GACNNNGTC 1 cut(s) 1051
VpaK11BI GGWCC 1 cut(s) 961
VspI ATTAAT 1 cut(s) 276
XagI CCTNNNNNAGG 1 cut(s) 485
XapI RAATTY 3 cut(s) 260, 550, 1019
XbaI TCTAGA 3 cut(s) 242, 386, 424
XceI RCATGY 1 cut(s) 1010
XmiI GTMKAC 1 cut(s) 292
XspI CTAG 6 cut(s) 132, 243, 387, 425, 528, 621
ZrmI AGTACT 1 cut(s) 619
Zsp2I ATGCAT 1 cut(s) 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.