Rh3DG127000
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000913
Physical Location & Seq
Forward (+)
10726203 .. 10729880
3678 bp
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UTR
Exon/CDS
Intron
Rh3DG127000.1

Sequence Viewer

Length: 648 bp
ATGATCTGTGCCTGCAGTGGATATCTGGCCCCAGAATATGCACTTTTGGGACAGCTTACAAAAAAAGCAGATGTATACAGCTTTGGGGTTCTCTTGCTTGAAATAATTAGTGGCAGAAGTAGTAGTAAGGCAGCCTTTGGTGACGAACTGATGGTTCTGGTGGAATGGACTTGGAAACTGAAAGAAGAAGAAAGGCTTATAGAAATTGTTGATCCAGAATTGACTGCATATCCAGAGGCTGAGGTAATGCGCTTCATTAAGGTTGCGCTCTTTTGTACTCAAGAAGCTTCGCATCAAAGGCCGGCCATGAAGCAAGTAGTGGAAATGCTTTCCAAAGAGGTACAGCTAAATGAGAAGCTACTAGTAGAACCAAGGCCAAGGAGGTATGCACCTCAACATGGAGGTGGTGCCAGTGGGGAGGGAACATCATCTTCTCAGTCAGATAAAGATAAACGCGGGAAATCATTGGCAAATCCTGATGTAACTTCCACCCAGTCGTATATATATGAATCTCAAACGCAAATGTTTCCCAGAAAATTTAAAGTCCCCAACTATCTATTTCATACTCCAAAGTCCATCCATGGAAGAGAGCCATTTGGCAGACTCAAAAGAGACGCTGCGCGCCTAACAATGCTCACATGGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

215

Amino Acids

24.31

Weight (kDa)

8.67

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 8 - 110 1.1e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 407
AccI GTMKAC 1 cut(s) 75
AccII CGCG 2 cut(s) 456, 622
AciI CCGC 1 cut(s) 456
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 1 cut(s) 536
AfaI GTAC 2 cut(s) 277, 342
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 1 cut(s) 101
AhlI ACTAGT 1 cut(s) 361
AluBI AGCT 5 cut(s) 55, 81, 287, 346, 358
AluI AGCT 5 cut(s) 55, 81, 287, 346, 358
Alw26I GTCTC 1 cut(s) 606
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 4 cut(s) 27, 299, 303, 374
ApeKI GCWGC 2 cut(s) 131, 617
ApoI RAATTY 1 cut(s) 536
AspLEI GCGC 4 cut(s) 252, 268, 622, 624
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 152
BanI GGYRCC 1 cut(s) 407
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 143, 604
BccI CCATC 2 cut(s) 145, 584
BcoDI GTCTC 1 cut(s) 606
BcuI ACTAGT 1 cut(s) 361
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 1 cut(s) 13
BisI GCNGC 2 cut(s) 132, 618
BlsI GCNGC 2 cut(s) 133, 619
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 2 cut(s) 30, 409
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 301
BmuI ACTGGG 1 cut(s) 487
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 371, 377, 580
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 2 cut(s) 411, 493
BseDI CCNNGG 3 cut(s) 371, 377, 580
BseGI GGATG 1 cut(s) 576
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMII CTCAG 2 cut(s) 231, 449
BseNI ACTGG 2 cut(s) 411, 493
BsePI GCGCGC 1 cut(s) 620
BseXI GCAGC 2 cut(s) 143, 604
Bsh1236I CGCG 2 cut(s) 456, 622
BshFI GGCC 4 cut(s) 29, 301, 305, 376
BshNI GGYRCC 1 cut(s) 407
BsiSI CCGG 1 cut(s) 302
BslFI GGGAC 2 cut(s) 63, 530
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 606
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 2 cut(s) 63, 530
BsnI GGCC 4 cut(s) 29, 301, 305, 376
Bsp143I GATC 2 cut(s) 3, 211
Bsp19I CCATGG 1 cut(s) 580
BspACI CCGC 1 cut(s) 456
BspANI GGCC 4 cut(s) 29, 301, 305, 376
BspCNI CTCAG 2 cut(s) 232, 448
BspFNI CGCG 2 cut(s) 456, 622
BspLI GGNNCC 2 cut(s) 30, 409
BspMAI CTGCAG 1 cut(s) 17
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 2 cut(s) 411, 493
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 3 cut(s) 371, 377, 580
BssHII GCGCGC 1 cut(s) 620
BssMI GATC 2 cut(s) 3, 211
BssNAI GTATAC 1 cut(s) 76
BssT1I CCWWGG 3 cut(s) 371, 377, 580
Bst1107I GTATAC 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 13, 303, 622
BstDEI CTNAG 2 cut(s) 240, 435
BstDSI CCRYGG 1 cut(s) 580
BstF5I GGATG 1 cut(s) 576
BstFNI CGCG 2 cut(s) 456, 622
BstHHI GCGC 4 cut(s) 252, 268, 622, 624
BstKTI GATC 2 cut(s) 6, 214
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 2 cut(s) 3, 211
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 13
BstUI CGCG 2 cut(s) 456, 622
BstV1I GCAGC 2 cut(s) 143, 604
BstZ17I GTATAC 1 cut(s) 76
BsuRI GGCC 4 cut(s) 29, 301, 305, 376
BtgI CCRYGG 1 cut(s) 580
BtsCI GGATG 1 cut(s) 576
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 22, 418
Cac8I GCNNGC 3 cut(s) 13, 303, 622
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 4 cut(s) 252, 268, 622, 624
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 1 cut(s) 623
Csp6I GTAC 2 cut(s) 276, 341
CviAII CATG 4 cut(s) 307, 398, 581, 639
CviQI GTAC 2 cut(s) 276, 341
DdeI CTNAG 2 cut(s) 240, 435
DpnI GATC 2 cut(s) 5, 213
DpnII GATC 2 cut(s) 3, 211
DraI TTTAAA 1 cut(s) 541
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
Eco130I CCWWGG 3 cut(s) 371, 377, 580
Eco32I GATATC 1 cut(s) 23
EcoRV GATATC 1 cut(s) 23
EcoT14I CCWWGG 3 cut(s) 371, 377, 580
ErhI CCWWGG 3 cut(s) 371, 377, 580
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 4 cut(s) 310, 401, 584, 642
FalI AAGNNNNNCTT 4 cut(s) 119, 151, 180, 212
FaqI GGGAC 2 cut(s) 63, 530
FatI CATG 4 cut(s) 306, 397, 580, 638
FauI CCCGC 1 cut(s) 449
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 2 cut(s) 132, 618
FokI GGATG 1 cut(s) 563
FseI GGCCGGCC 1 cut(s) 305
Fsp4HI GCNGC 2 cut(s) 132, 618
FspBI CTAG 1 cut(s) 362
GlaI GCGC 4 cut(s) 251, 267, 621, 623
GluI GCNGC 2 cut(s) 132, 618
HaeIII GGCC 4 cut(s) 29, 301, 305, 376
HapII CCGG 1 cut(s) 302
HgaI GACGC 1 cut(s) 623
HhaI GCGC 4 cut(s) 252, 268, 622, 624
Hin1II CATG 4 cut(s) 310, 401, 584, 642
Hin6I GCGC 4 cut(s) 250, 266, 620, 622
HinP1I GCGC 4 cut(s) 250, 266, 620, 622
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 2 cut(s) 509, 603
HpaII CCGG 1 cut(s) 302
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 4 cut(s) 215, 233, 281, 476
Hpy8I GTNNAC 1 cut(s) 76
HpyCH4V TGCA 4 cut(s) 15, 41, 227, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpyF3I CTNAG 2 cut(s) 240, 435
Hsp92II CATG 4 cut(s) 310, 401, 584, 642
HspAI GCGC 4 cut(s) 250, 266, 620, 622
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Kzo9I GATC 2 cut(s) 3, 211
Lsp1109I GCAGC 2 cut(s) 143, 604
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 140, 481
MalI GATC 2 cut(s) 5, 213
MboI GATC 2 cut(s) 3, 211
MboII GAAGA 4 cut(s) 197, 200, 423, 597
MluCI AATT 4 cut(s) 105, 204, 218, 536
MlyI GAGTC 1 cut(s) 597
MnlI CCTC 7 cut(s) 229, 235, 331, 375, 395, 402, 412
MroNI GCCGGC 1 cut(s) 301
MseI TTAA 3 cut(s) 258, 540, 646
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 1 cut(s) 302
MvnI CGCG 2 cut(s) 456, 622
MwoI GCNNNNNNNGC 1 cut(s) 298
NaeI GCCGGC 1 cut(s) 303
NcoI CCATGG 1 cut(s) 580
NdeII GATC 2 cut(s) 3, 211
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 4 cut(s) 310, 401, 584, 642
NlaIV GGNNCC 2 cut(s) 30, 409
NmuCI GTSAC 1 cut(s) 140
PauI GCGCGC 1 cut(s) 620
PdiI GCCGGC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 509
PkrI GCNGC 2 cut(s) 133, 619
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
PspN4I GGNNCC 2 cut(s) 30, 409
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 17
PstNI CAGNNNCTG 1 cut(s) 239
PteI GCGCGC 1 cut(s) 620
RigI GGCCGGCC 1 cut(s) 305
RsaI GTAC 2 cut(s) 277, 342
RsaNI GTAC 2 cut(s) 276, 341
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 3 cut(s) 258, 540, 646
SatI GCNGC 2 cut(s) 132, 618
Sau3AI GATC 2 cut(s) 3, 211
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 597
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
SpeI ACTAGT 1 cut(s) 361
Sse9I AATT 4 cut(s) 105, 204, 218, 536
SsiI CCGC 1 cut(s) 456
SspMI CTAG 1 cut(s) 362
StyI CCWWGG 3 cut(s) 371, 377, 580
TasI AATT 4 cut(s) 105, 204, 218, 536
TatI WGTACW 1 cut(s) 275
TfiI GAWTC 1 cut(s) 509
Tru1I TTAA 3 cut(s) 258, 540, 646
Tru9I TTAA 3 cut(s) 258, 540, 646
TscAI CASTG 2 cut(s) 22, 418
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 2 cut(s) 131, 617
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 4 cut(s) 244, 323, 522, 551
TspRI CASTG 2 cut(s) 22, 418
XapI RAATTY 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.