pycom04g13650
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000913
Physical Location & Seq
Reverse (-)
16642597 .. 16645560
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13650.5

Sequence Viewer

Length: 1158 bp
ATGGTTTGTGGTTGCTTCTCTGTGCTGAAATGGCGTAAAGAAGAGAGAGCTTCCTCGTCTTCCTTGCAGGAGCAAGGGATTATCAACAACGTAAGGCTTTTCTCATATAATTCTCTGAGATCAGCTACTAGGAACTTTCATCCATCGAGCAGGATAGGTGGAGGAGGTTATGGAGTTGTCTATAGGGGAATTCTAAGAGACGATGCCCAAGTTGCGATCAAATGTCTTTCTGCAGAATCTAGACAAGGAGCAAATGAATTTTTGACAGAGATTAATGTGATATCGCGTATACGACATCCAAACCTTGTTGAGCTCCTTGGTTGCTGTGTGGAGGACAATCATCGGATATTGGTATATGAATATCTGGAGAACAACAGTCTTTCTAAATCTTTGCTTGGTTCGAGAAGTAAATATGTTTTTCTAGATTGGCCAACGAGAGTTTCGATTTGCCTTGGTACAGCTACCGGTCTTGCATTCCTTCACGAGGAAGCTGAACAGCAAATTGTCCACAGAGATATCAAGGCTAGCAATATACTTCTTGATGCAAATTTTCGTCCTAAAATTGGAGATTTTGGGCTGGCAAAACTTTTTCCTGATAATGTCACTCATCTTAGTACTAGAGTAGCTGGAACGGAGGGATATCTCGCTCCAGAATATGCGCTTTTGGGACAACTTACGAAGAAAGCAGATGTGTACAGCTTTGGGGTGCTCTTGCTTGAGATAATTAGTGGCAGAAGTAGTAGTAAGGCAGCCTTCGGGGAGCAACTGCTGGTTCTCGTCGAATGGGTATGGAAACTAAGAGAAGAAGAAAGGCTCCTGGAACTTGTTGATCCAGAATTGACTGCATATCCAGAGGCCGAGGTTATGCGCTTCATCAAGGTTGCACTGTTTTGTACTCAAGCCACTGCACAACAGAGACCAACAATGAAGCAAGTTGTGGAAATGCTTTCCAAAGAGGTCCACCTAAACGAGAAGGCACTAACAAAACCAAGGCCGAGGATGCATGCATCTGGAAAATTTGGCGGTACCAGTTCGTTGGAGGAGACATCGTCATCATCTCATTCAGAGAAACGCAAGAAATCAACGGCAAACCCGGATGTAACTTCCACCAGCTCGTTGATCTTTGATGATTCTGAAACACAAATACTTCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

386

Amino Acids

42.99

Weight (kDa)

8.09

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1025
AccB1I GGYRCC 1 cut(s) 1025
AccI GTMKAC 1 cut(s) 289
AccII CGCG 1 cut(s) 286
AciI CCGC 1 cut(s) 1023
AclWI GGATC 1 cut(s) 824
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 4 cut(s) 189, 257, 547, 1016
AfaI GTAC 5 cut(s) 457, 616, 695, 895, 1027
AfiI CCNNNNNNNGG 2 cut(s) 484, 563
AgeI ACCGGT 1 cut(s) 464
AjnI CCWGG 1 cut(s) 816
AjuI GAANNNNNNNTTGG 2 cut(s) 424, 456
AluBI AGCT 8 cut(s) 50, 125, 313, 461, 491, 626, 699, 1113
AluI AGCT 8 cut(s) 50, 125, 313, 461, 491, 626, 699, 1113
Alw21I GWGCWC 2 cut(s) 315, 711
Alw26I GTCTC 3 cut(s) 192, 910, 1037
AlwI GGATC 1 cut(s) 824
AoxI GGCC 3 cut(s) 428, 855, 992
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 4 cut(s) 189, 257, 547, 1016
AseI ATTAAT 1 cut(s) 273
AsiGI ACCGGT 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 1025
AspLEI GCGC 2 cut(s) 661, 870
AspS9I GGNCC 1 cut(s) 958
AsuC2I CCSGG 1 cut(s) 1094
AsuNHI GCTAGC 1 cut(s) 524
AvaII GGWCC 1 cut(s) 958
BalI TGGCCA 1 cut(s) 430
BanI GGYRCC 1 cut(s) 1025
BanII GRGCYC 1 cut(s) 315
BauI CACGAG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 315, 711
BbvI GCAGC 1 cut(s) 761
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 1101
BciT130I CCWGG 1 cut(s) 818
BcnI CCSGG 1 cut(s) 1094
BcoDI GTCTC 3 cut(s) 192, 910, 1037
BfaI CTAG 5 cut(s) 129, 240, 422, 525, 618
BfmI CTRYAG 2 cut(s) 181, 231
BisI GCNGC 1 cut(s) 750
BlsI GCNGC 1 cut(s) 751
BmcAI AGTACT 1 cut(s) 616
Bme1390I CCNGG 2 cut(s) 818, 1094
Bme18I GGWCC 1 cut(s) 958
BmgT120I GGNCC 1 cut(s) 958
BmiI GGNNCC 2 cut(s) 815, 1027
BmrFI CCNGG 2 cut(s) 818, 1094
BmsI GCATC 4 cut(s) 193, 532, 990, 1016
BmtI GCTAGC 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 2 cut(s) 386, 633
BpuEI CTTGAG 2 cut(s) 737, 882
BpuMI CCSGG 1 cut(s) 1094
BsaI GGTCTC 1 cut(s) 910
BsaJI CCNNGG 5 cut(s) 316, 451, 858, 989, 995
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bsc4I CCNNNNNNNGG 2 cut(s) 484, 563
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 1 cut(s) 1029
BseBI CCWGG 1 cut(s) 818
BseDI CCNNGG 5 cut(s) 316, 451, 858, 989, 995
BseGI GGATG 4 cut(s) 139, 295, 1005, 1102
BseLI CCNNNNNNNGG 2 cut(s) 484, 563
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 1 cut(s) 1029
BseRI GAGGAG 2 cut(s) 177, 1055
BseXI GCAGC 1 cut(s) 761
BsgI GTGCAG 1 cut(s) 891
Bsh1236I CGCG 1 cut(s) 286
BshFI GGCC 3 cut(s) 430, 857, 994
BshNI GGYRCC 1 cut(s) 1025
BshTI ACCGGT 1 cut(s) 464
BsiHKAI GWGCWC 2 cut(s) 315, 711
BsiSI CCGG 2 cut(s) 465, 1094
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 2 cut(s) 484, 563
BsmAI GTCTC 3 cut(s) 192, 910, 1037
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 681
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 3 cut(s) 430, 857, 994
Bso31I GGTCTC 1 cut(s) 910
Bsp1286I GDGCHC 2 cut(s) 315, 711
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 4 cut(s) 119, 216, 829, 1119
BspACI CCGC 1 cut(s) 1023
BspANI GGCC 3 cut(s) 430, 857, 994
BspCNI CTCAG 1 cut(s) 108
BspFNI CGCG 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 815, 1027
BspMAI CTGCAG 1 cut(s) 235
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 1 cut(s) 824
BspT107I GGYRCC 1 cut(s) 1025
BspTNI GGTCTC 1 cut(s) 910
BsrFI RCCGGY 1 cut(s) 464
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 1 cut(s) 1029
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 5 cut(s) 316, 451, 858, 989, 995
BssMI GATC 4 cut(s) 119, 216, 829, 1119
BssNAI GTATAC 1 cut(s) 290
BssSI CACGAG 1 cut(s) 482
BssT1I CCWWGG 3 cut(s) 316, 451, 989
Bst1107I GTATAC 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 818
Bst4CI ACNGT 2 cut(s) 377, 888
Bst6I CTCTTC 1 cut(s) 36
BstAUI TGTACA 1 cut(s) 693
BstC8I GCNNGC 3 cut(s) 526, 579, 1005
BstDEI CTNAG 4 cut(s) 116, 194, 611, 797
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 4 cut(s) 139, 295, 1005, 1102
BstFNI CGCG 1 cut(s) 286
BstHHI GCGC 2 cut(s) 661, 870
BstKTI GATC 4 cut(s) 122, 219, 832, 1122
BstMAI GTCTC 3 cut(s) 192, 910, 1037
BstMBI GATC 4 cut(s) 119, 216, 829, 1119
BstMWI GCNNNNNNNGC 3 cut(s) 31, 212, 1000
BstNI CCWGG 1 cut(s) 818
BstNSI RCATGY 1 cut(s) 1007
BstSCI CCNGG 2 cut(s) 816, 1092
BstSFI CTRYAG 2 cut(s) 181, 231
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 761
BstV2I GAAGAC 1 cut(s) 51
BstXI CCANNNNNNTGG 1 cut(s) 1036
BstZ17I GTATAC 1 cut(s) 290
BsuRI GGCC 3 cut(s) 430, 857, 994
BtsCI GGATG 4 cut(s) 139, 295, 1005, 1102
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 2 cut(s) 884, 903
Cac8I GCNNGC 3 cut(s) 526, 579, 1005
CfoI GCGC 2 cut(s) 661, 870
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 958
Csp6I GTAC 5 cut(s) 456, 615, 694, 894, 1026
CspAI ACCGGT 1 cut(s) 464
CviAII CATG 1 cut(s) 1004
CviQI GTAC 5 cut(s) 456, 615, 694, 894, 1026
DdeI CTNAG 4 cut(s) 116, 194, 611, 797
DpnI GATC 4 cut(s) 121, 218, 831, 1121
DpnII GATC 4 cut(s) 119, 216, 829, 1119
EaeI YGGCCR 1 cut(s) 428
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 3 cut(s) 316, 451, 989
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 910
Eco32I GATATC 3 cut(s) 282, 517, 641
Eco47I GGWCC 1 cut(s) 958
Eco53kI GAGCTC 1 cut(s) 313
EcoICRI GAGCTC 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoRI GAATTC 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 816
EcoRV GATATC 3 cut(s) 282, 517, 641
EcoT14I CCWWGG 3 cut(s) 316, 451, 989
EcoT22I ATGCAT 2 cut(s) 1005, 1009
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 3 cut(s) 316, 451, 989
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 1 cut(s) 1007
FalI AAGNNNNNCTT 2 cut(s) 737, 769
FaqI GGGAC 1 cut(s) 681
FatI CATG 1 cut(s) 1003
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 750
FokI GGATG 4 cut(s) 126, 282, 1012, 1109
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 750
FspBI CTAG 5 cut(s) 129, 240, 422, 525, 618
GlaI GCGC 2 cut(s) 660, 869
GluI GCNGC 1 cut(s) 750
GsuI CTGGAG 2 cut(s) 386, 633
HaeIII GGCC 3 cut(s) 430, 857, 994
HapII CCGG 2 cut(s) 465, 1094
HhaI GCGC 2 cut(s) 661, 870
Hin1II CATG 1 cut(s) 1007
Hin6I GCGC 2 cut(s) 659, 868
HinP1I GCGC 2 cut(s) 659, 868
HinfI GANTC 2 cut(s) 236, 1130
HpaII CCGG 2 cut(s) 465, 1094
Hpy166II GTNNAC 4 cut(s) 290, 508, 694, 961
Hpy188I TCNGA 4 cut(s) 117, 345, 1066, 1135
Hpy8I GTNNAC 4 cut(s) 290, 508, 694, 961
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 3 cut(s) 488, 763, 967
HpyCH4III ACNGT 2 cut(s) 377, 888
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 9 cut(s) 67, 233, 473, 545, 845, 884, 908, 1003, 1007
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 212, 1000
HpyF3I CTNAG 4 cut(s) 116, 194, 611, 797
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 1 cut(s) 1007
HspAI GCGC 2 cut(s) 659, 868
KpnI GGTACC 1 cut(s) 1029
Kzo9I GATC 4 cut(s) 119, 216, 829, 1119
LmnI GCTCC 6 cut(s) 70, 248, 318, 652, 760, 819
Lsp1109I GCAGC 1 cut(s) 761
LweI GCATC 4 cut(s) 193, 532, 990, 1016
MaeI CTAG 5 cut(s) 129, 240, 422, 525, 618
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 601, 1099
MalI GATC 4 cut(s) 121, 218, 831, 1121
MboI GATC 4 cut(s) 119, 216, 829, 1119
MboII GAAGA 5 cut(s) 51, 53, 691, 815, 818
MhlI GDGCHC 2 cut(s) 315, 711
MlsI TGGCCA 1 cut(s) 430
MluCI AATT 9 cut(s) 109, 189, 257, 501, 547, 561, 723, 836, 1016
MluNI TGGCCA 1 cut(s) 430
MmeI TCCRAC 1 cut(s) 1017
Mox20I TGGCCA 1 cut(s) 430
Mph1103I ATGCAT 2 cut(s) 1005, 1009
MscI TGGCCA 1 cut(s) 430
MseI TTAA 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 430
MspI CCGG 2 cut(s) 465, 1094
MspR9I CCNGG 2 cut(s) 818, 1094
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 1 cut(s) 818
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 3 cut(s) 31, 212, 1000
NciI CCSGG 1 cut(s) 1094
NdeII GATC 4 cut(s) 119, 216, 829, 1119
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 1 cut(s) 1007
NlaIV GGNNCC 2 cut(s) 815, 1027
NmeAIII GCCGAG 2 cut(s) 883, 1020
NmuCI GTSAC 1 cut(s) 601
NsiI ATGCAT 2 cut(s) 1005, 1009
NspI RCATGY 1 cut(s) 1007
PaeI GCATGC 1 cut(s) 1007
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PctI GAATGC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 236, 1130
PflFI GACNNNGTC 1 cut(s) 1048
PfoI TCCNGGA 1 cut(s) 816
PinAI ACCGGT 1 cut(s) 464
PkrI GCNGC 1 cut(s) 751
PshBI ATTAAT 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 816
PspGI CCWGG 1 cut(s) 816
PspN4I GGNNCC 2 cut(s) 815, 1027
PspPI GGNCC 1 cut(s) 958
PstI CTGCAG 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 1048
RsaI GTAC 5 cut(s) 457, 616, 695, 895, 1027
RsaNI GTAC 5 cut(s) 456, 615, 694, 894, 1026
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 1 cut(s) 750
Sau3AI GATC 4 cut(s) 119, 216, 829, 1119
Sau96I GGNCC 1 cut(s) 958
ScaI AGTACT 1 cut(s) 616
ScrFI CCNGG 2 cut(s) 818, 1094
SduI GDGCHC 2 cut(s) 315, 711
SfaNI GCATC 4 cut(s) 193, 532, 990, 1016
SfcI CTRYAG 2 cut(s) 181, 231
SinI GGWCC 1 cut(s) 958
SmlI CTYRAG 2 cut(s) 716, 897
SmoI CTYRAG 2 cut(s) 716, 897
SphI GCATGC 1 cut(s) 1007
Sse9I AATT 9 cut(s) 109, 189, 257, 501, 547, 561, 723, 836, 1016
SsiI CCGC 1 cut(s) 1023
SspMI CTAG 5 cut(s) 129, 240, 422, 525, 618
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 816, 1092
StyI CCWWGG 3 cut(s) 316, 451, 989
TaaI ACNGT 2 cut(s) 377, 888
TaiI ACGT 1 cut(s) 93
TaqI TCGA 4 cut(s) 146, 401, 443, 780
TasI AATT 9 cut(s) 109, 189, 257, 501, 547, 561, 723, 836, 1016
TatI WGTACW 3 cut(s) 614, 693, 893
TfiI GAWTC 2 cut(s) 236, 1130
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 2 cut(s) 891, 910
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 5 cut(s) 128, 270, 372, 862, 941
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 2 cut(s) 891, 910
Tth111I GACNNNGTC 1 cut(s) 1048
VpaK11BI GGWCC 1 cut(s) 958
VspI ATTAAT 1 cut(s) 273
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 4 cut(s) 189, 257, 547, 1016
XbaI TCTAGA 2 cut(s) 239, 421
XceI RCATGY 1 cut(s) 1007
XmiI GTMKAC 1 cut(s) 289
XspI CTAG 5 cut(s) 129, 240, 422, 525, 618
ZrmI AGTACT 1 cut(s) 616
Zsp2I ATGCAT 2 cut(s) 1005, 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.