Rroxscaffold_6G00416710

Flocculation protein FLO11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000913
Physical Location & Seq
Reverse (-)
38628366 .. 38635807
7442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416710.1

Sequence Viewer

Length: 2769 bp
ATGGAAAGAAGCTTCGGGAAGGTGGTCAATGCCAACGACAGAGACGAAGAGCTCGCTCTGTTTCTCGAGATGCGGCGCCGCGACAAGGATAAGGAGAAAAATGGAAACTTTCTGCTCCTTCCGACCAAGAACGGCGGCGACGAGCTCGATGCTGCTGCTGCTGCTCCGATTGAGTATGATAAGGCGATGTACAAGATTAATGGGTCGGCGGCGCCGGAGCGGAGGAGTAGAGTGGATGAGTTTTTGAACTCGGAGAATGAGAAATCGGAGTACGATTGGTTAATTACTCCACCTGCTACCCCATTATTTCCATCCTTGGAGATGGAACCACAAAAGACTTCAGTTAGTCAGATTGAGGAAACTAATGCGCGTGATTCTGCTCCTCCAAAATCTAGGCTAGCAAACACAAAGTTAGAAACTGCTTCAGACAGCAAAATAGCATCTAAGCAGCCAACATTGCCATCACGATCGAATTCTTCCAGTACTAGTAACAGGAGGCCCTCACCATCAAGAGGGCCAACAGCTGCTCCTCGTAGATCTGCAACACCAACTGGACGGCCCACCCCATTACCTTCATCAACCAAGCCATCAAGGTCCTCCACGCCCAATTCACGAGCCACCTTACCATCTGCTAAGCCAGTGGCTCCTCCAGTGAGGTCCTCAACTCCTTCTAGATCCACCGTGCGTTCTTCTACACCAACTGGCAGATCTTCTGTCCCAGCTTCTAAGACAATATCAAGATCAGCAACACCAACTCGCCTACCAAGTTCATCAAGTACACCTGCACCGTCTGCTCCTCGTGGCCGTTCTTCTTCAGTATCAAAGTCAGCTCCCCCAAGTTCGAAAAATCCTGCACCATCACGTGGAAGTTCTCCAACAGTAAAATCTAGGTCCTCGAAATCCTCAGATATGCCTGCTTTCTCACTTGATGCTCCACCAAATTTGAGAACCACTTTGCCACAAAGGCCAAATTCAGCCTCTAGGGCTCGTCCTGGGGCACCAAGTGTTAGATCATCTTCTGTTGGAGCTGTTTCTAGTGGAAAACCTAGACAGCAATCATGTTCACCTTCTAGAGGAAGGGCTTCACATGGCACTGCCAGTACTAATGGGTACTCTCGTCAAGTCACAAGTAAATCATCTCCCAATGACAGTGATGATGTAAATCCTGTACTTATGGGGACCCAAAGTGTTGAGAGAGTAGTGAACCAGAGGAAACTGGCACCACCAAAGCAGTATGACCATTCTACTCAGAAGAATCCTGATGGGAAGTCTTTGTCCTCTGACAGCTTAGGCTTTGGAAGAACACTTTCAAAGAAATCCTTCGATATGGCTATGAGACACATGGATATAAGGCGAAGCGTGCAAGATAACCTACATCCTGTTCTGACAAAAGTTCCAGCCTCCTCTGTGTACAGTGTCCGGACAAGATCAACAAAAAGCAAGACTACTAATGCTACGGATTCTCCTCTTGCCACATGCAGCAATGCTAGCTCTGAACCAAGTGTCAACAATATTTCTGCATACTTTGAAGGTTGTGAAATTGAGGACAATGATCATGGAAGTGAGAAAGGAAACTCCTCTCCTGCATGCCAGCAAGGCAGTGCCGGCCGTTTCAGTGGGGAAGGACATATGGTTTCAGTGGGGAAGGACATATGTCAGTGGGGAAGGACATATGGTTTCAGTGGGGATGTGGAACAAATGAATACACTTTTGACTAGAAACATGAGCGCTCCCATAATTGCTTTGGTGTGCTCAATTGGTGCAAAGGAAAGAAGAATTTGGAACCTTCCAGCCAAGCCCAAGGGAGTTTTAAGAGATGACACAGAAGTTGCGATCAAGTGTCTTTCTTCAGACTCTAGACAAGGAGCAAATGAATTTTTGACAGAGATCAACACGATATCAAGAATACAACACCCAAACCTTGTCGAGCTTATTGGCTGCTGTGTGGAGGACGATCACCGAATATTGGTATATGAATATCTGGAGAACAACAGTCTTGCAAGTTCTTTGCTTGGTTCAAAAAGTCATGTTCCTCTAGATTGGCCCCAGAGAGCAGCAATTTGCCTGGGTACAGCTACTGGTCTTGCTTTTCTTCATGACGAAGCTGAACAACATATTGTGCACAGAGATATCAAGGCTAGCAATATACTTCTCGATGCAAACCTTCGTCCTAAAATTGGAGATTTTGGGCTGGCAAAACTTTTTCCTGATAATGTCACTCATCTTAGTACTAGAGTGGCAGGAACTGTTGGATACCTAGCCCCAGAATATGCACTTTTGGGACAGCTTACAAAAAAAGCAGATGTATACAGCTTTGGGGTTCTCTTGCTTGAAATAATTAGTGGCAGAAGTAGTAGTAAGGCAGCCTTTGGTGACGAACTGATGGTTCTGGTGGAATGGACTTGGAAACTGAAAGAAGAAGAAAGGCTTATAGAAATTGTTGATCCAGAATTGACTGCATATCCAGAGGCTGAGGTAATGCGCTTCATTAAGGTTGCGCTCTTTTGTACTCAAGAAGCTTCGCATCAAAGGCCGGCCATGAAGCAAGTAGTGGAAATGCTTTCCAAAGAGGTACAGCTAAATGAGAAGCTACTAGTAGAACCAAGGCCAAGGAGGTATGCATCTCAACATGGAGGTGGTGCCAGTGGGGAGGGAACATCATCTTCTCAGTCAGATAAAGATAAACGTGGGAAATCATTGGCAAATCCTGATGTAACTTCCACCCAGTCGTATATATATGAATCTCAAACGCAAATGTTTCCCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

922

Amino Acids

99.82

Weight (kDa)

9.43

Isoelectric Point (pI)

60.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 606 - 854 2.2e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 606 - 852 6.1e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 301, 790
Acc36I ACCTGC 2 cut(s) 301, 790
AccB1I GGYRCC 5 cut(s) 75, 211, 997, 1219, 2639
AccB7I CCANNNNNTGG 1 cut(s) 863
AccBSI CCGCTC 1 cut(s) 220
AccI GTMKAC 1 cut(s) 2307
AccII CGCG 2 cut(s) 81, 370
AccIII TCCGGA 1 cut(s) 1419
AciI CCGC 5 cut(s) 73, 79, 135, 209, 220
AclWI GGATC 2 cut(s) 669, 2438
AcoI YGGCCR 3 cut(s) 802, 1606, 2535
AcsI RAATTY 5 cut(s) 472, 940, 970, 1776, 1874
AcuI CTGAAG 4 cut(s) 324, 408, 798, 1833
AcvI CACGTG 1 cut(s) 863
AcyI GRCGYC 2 cut(s) 76, 212
AdeI CACNNNGTG 1 cut(s) 1004
AfeI AGCGCT 1 cut(s) 1729
AfiI CCNNNNNNNGG 6 cut(s) 85, 512, 863, 1073, 1966, 2177
AgsI TTSAA 5 cut(s) 247, 1311, 1529, 2019, 2333
AhlI ACTAGT 2 cut(s) 485, 2593
AjnI CCWGG 3 cut(s) 991, 2064, 2762
Alw21I GWGCWC 4 cut(s) 54, 147, 1754, 2124
Alw26I GTCTC 2 cut(s) 36, 1330
Alw44I GTGCAC 1 cut(s) 2120
AlwI GGATC 2 cut(s) 669, 2438
AlwNI CAGNNNCTG 3 cut(s) 2078, 2246, 2471
Ama87I CYCGRG 1 cut(s) 65
Aor13HI TCCGGA 1 cut(s) 1419
Aor51HI AGCGCT 1 cut(s) 1729
ApaLI GTGCAC 1 cut(s) 2120
ApoI RAATTY 5 cut(s) 472, 940, 970, 1776, 1874
AseI ATTAAT 1 cut(s) 198
Asp700I GAANNNNTTC 3 cut(s) 1081, 1306, 1319
AspLEI GCGC 6 cut(s) 78, 214, 370, 1730, 2484, 2500
AspS9I GGNCC 8 cut(s) 498, 515, 558, 594, 657, 891, 1179, 2043
AsuHPI GGTGA 4 cut(s) 495, 1056, 1949, 2384
AsuII TTCGAA 1 cut(s) 842
AsuNHI GCTAGC 3 cut(s) 397, 1487, 2138
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 4 cut(s) 594, 657, 891, 1179
BaeGI GKGCMC 2 cut(s) 1000, 2124
BaeI ACNNNNGTAYC 2 cut(s) 1102, 1135
BanI GGYRCC 5 cut(s) 75, 211, 997, 1219, 2639
BanII GRGCYC 3 cut(s) 54, 147, 988
BauI CACGAG 2 cut(s) 612, 798
BbrPI CACGTG 1 cut(s) 863
Bbv12I GWGCWC 4 cut(s) 54, 147, 1754, 2124
BbvCI CCTCAGC 1 cut(s) 2472
BccI CCATC 9 cut(s) 316, 319, 469, 514, 595, 634, 865, 1256, 2377
BceAI ACGGC 4 cut(s) 148, 572, 789, 1593
BcgI CGANNNNNNTGC 4 cut(s) 131, 137, 165, 171
BciT130I CCWGG 3 cut(s) 993, 2066, 2764
BciVI GTATCC 1 cut(s) 2246
BclI TGATCA 1 cut(s) 1552
BcoDI GTCTC 2 cut(s) 36, 1330
BcuI ACTAGT 2 cut(s) 485, 2593
BfoI RGCGCY 3 cut(s) 79, 215, 1731
BfuAI ACCTGC 2 cut(s) 301, 790
BfuI GTATCC 1 cut(s) 2246
BglI GCCNNNNNGGC 3 cut(s) 964, 983, 1596
BglII AGATCT 2 cut(s) 536, 707
BlpI GCTNAGC 1 cut(s) 633
BmcAI AGTACT 3 cut(s) 484, 1102, 2230
Bme1390I CCNGG 3 cut(s) 993, 2066, 2764
Bme18I GGWCC 4 cut(s) 594, 657, 891, 1179
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 8 cut(s) 498, 515, 558, 594, 657, 891, 1179, 2043
BmrFI CCNGG 3 cut(s) 993, 2066, 2764
BmrI ACTGGG 1 cut(s) 2719
BmsI GCATC 7 cut(s) 60, 139, 449, 919, 2146, 2533, 2630
BmtI GCTAGC 3 cut(s) 401, 1491, 2142
BmuI ACTGGG 1 cut(s) 2719
BoxI GACNNNNGTC 1 cut(s) 1653
BpmI CTGGAG 2 cut(s) 633, 2003
Bpu10I CCTNAGC 2 cut(s) 1288, 2472
Bpu1102I GCTNAGC 1 cut(s) 633
Bpu14I TTCGAA 1 cut(s) 842
BpuEI CTTGAG 1 cut(s) 2496
BsaAI YACGTR 1 cut(s) 863
BsaBI GATNNNNATC 1 cut(s) 1161
BsaHI GRCGYC 2 cut(s) 76, 212
BsaJI CCNNGG 7 cut(s) 315, 992, 1800, 2065, 2603, 2609, 2762
BsaWI WCCGGW 1 cut(s) 1419
BsaXI ACNNNNNCTCC 4 cut(s) 511, 541, 1447, 1477
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 512, 863, 1073, 1966, 2177
Bse118I RCCGGY 2 cut(s) 1604, 2533
Bse3DI GCAATG 2 cut(s) 455, 1489
Bse8I GATNNNNATC 1 cut(s) 1161
BseAI TCCGGA 1 cut(s) 1419
BseBI CCWGG 3 cut(s) 993, 2066, 2764
BseDI CCNNGG 7 cut(s) 315, 992, 1800, 2065, 2603, 2609, 2762
BseGI GGATG 4 cut(s) 241, 311, 1375, 1693
BseJI GATNNNNATC 1 cut(s) 1161
BseLI CCNNNNNNNGG 6 cut(s) 85, 512, 863, 1073, 1966, 2177
BseMI GCAATG 2 cut(s) 455, 1489
BseMII CTCAG 4 cut(s) 918, 1262, 2463, 2681
BseRI GAGGAG 8 cut(s) 238, 372, 519, 636, 786, 1393, 1455, 1567
BseSI GKGCMC 2 cut(s) 1000, 2124
BseX3I CGGCCG 1 cut(s) 1606
BseYI CCCAGC 1 cut(s) 718
BsgI GTGCAG 2 cut(s) 768, 837
Bsh1236I CGCG 2 cut(s) 81, 370
Bsh1285I CGRYCG 2 cut(s) 470, 1609
BshNI GGYRCC 5 cut(s) 75, 211, 997, 1219, 2639
BsiEI CGRYCG 2 cut(s) 470, 1609
BsiHKAI GWGCWC 4 cut(s) 54, 147, 1754, 2124
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 4 cut(s) 215, 1420, 1605, 2534
BslFI GGGAC 3 cut(s) 701, 1192, 2295
BslI CCNNNNNNNGG 6 cut(s) 85, 512, 863, 1073, 1966, 2177
BsmAI GTCTC 2 cut(s) 36, 1330
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 3 cut(s) 701, 1192, 2295
BsoBI CYCGRG 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 842
Bsp1286I GDGCHC 6 cut(s) 54, 147, 988, 1000, 1754, 2124
Bsp13I TCCGGA 1 cut(s) 1419
Bsp1407I TGTACA 2 cut(s) 189, 1410
Bsp1720I GCTNAGC 1 cut(s) 633
BspACI CCGC 5 cut(s) 73, 79, 135, 209, 220
BspCNI CTCAG 4 cut(s) 917, 1261, 2464, 2680
BspEI TCCGGA 1 cut(s) 1419
BspFNI CGCG 2 cut(s) 81, 370
BspHI TCATGA 1 cut(s) 2095
BspMI ACCTGC 2 cut(s) 301, 790
BspOI GCTAGC 3 cut(s) 401, 1491, 2142
BspPI GGATC 2 cut(s) 669, 2438
BspQI GCTCTTC 1 cut(s) 42
BspT104I TTCGAA 1 cut(s) 842
BspT107I GGYRCC 5 cut(s) 75, 211, 997, 1219, 2639
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 2 cut(s) 455, 1489
BsrFI RCCGGY 2 cut(s) 1604, 2533
BsrGI TGTACA 2 cut(s) 189, 1410
BssAI RCCGGY 2 cut(s) 1604, 2533
BssECI CCNNGG 7 cut(s) 315, 992, 1800, 2065, 2603, 2609, 2762
BssNAI GTATAC 1 cut(s) 2308
BssNI GRCGYC 2 cut(s) 76, 212
BssSI CACGAG 2 cut(s) 612, 798
BssT1I CCWWGG 4 cut(s) 315, 1800, 2603, 2609
Bst1107I GTATAC 1 cut(s) 2308
Bst2BI CACGAG 2 cut(s) 612, 798
Bst2UI CCWGG 3 cut(s) 993, 2066, 2764
Bst4CI ACNGT 7 cut(s) 682, 789, 880, 1151, 1415, 1994, 2248
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 2 cut(s) 76, 212
BstAPI GCANNNNNTGC 1 cut(s) 791
BstAUI TGTACA 2 cut(s) 189, 1410
BstBAI YACGTR 1 cut(s) 863
BstBI TTCGAA 1 cut(s) 842
BstDEI CTNAG 9 cut(s) 444, 633, 726, 904, 1248, 1288, 2225, 2472, 2667
BstENI CCTNNNNNAGG 1 cut(s) 1071
BstF5I GGATG 4 cut(s) 241, 311, 1375, 1693
BstFNI CGCG 2 cut(s) 81, 370
BstH2I RGCGCY 3 cut(s) 79, 215, 1731
BstHHI GCGC 6 cut(s) 78, 214, 370, 1730, 2484, 2500
BstMAI GTCTC 2 cut(s) 36, 1330
BstMCI CGRYCG 2 cut(s) 470, 1609
BstNI CCWGG 3 cut(s) 993, 2066, 2764
BstNSI RCATGY 2 cut(s) 1479, 1590
BstPAI GACNNNNGTC 1 cut(s) 1653
BstSCI CCNGG 3 cut(s) 991, 2064, 2762
BstSLI GKGCMC 2 cut(s) 1000, 2124
BstUI CGCG 2 cut(s) 81, 370
BstX2I RGATCY 3 cut(s) 536, 674, 707
BstYI RGATCY 3 cut(s) 536, 674, 707
BstZ17I GTATAC 1 cut(s) 2308
BstZI CGGCCG 1 cut(s) 1606
BsuI GTATCC 1 cut(s) 2246
BtgZI GCGATG 1 cut(s) 200
BtsCI GGATG 4 cut(s) 241, 311, 1375, 1693
BtsI GCAGTG 2 cut(s) 1092, 1606
BveI ACCTGC 2 cut(s) 301, 790
CaiI CAGNNNCTG 3 cut(s) 2078, 2246, 2471
CciI TCATGA 1 cut(s) 2095
CfoI GCGC 6 cut(s) 78, 214, 370, 1730, 2484, 2500
Cfr10I RCCGGY 2 cut(s) 1604, 2533
Cfr13I GGNCC 8 cut(s) 498, 515, 558, 594, 657, 891, 1179, 2043
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
DdeI CTNAG 9 cut(s) 444, 633, 726, 904, 1248, 1288, 2225, 2472, 2667
DinI GGCGCC 2 cut(s) 77, 213
DraIII CACNNNGTG 1 cut(s) 1004
EaeI YGGCCR 3 cut(s) 802, 1606, 2535
EagI CGGCCG 1 cut(s) 1606
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 2 cut(s) 52, 145
EclXI CGGCCG 1 cut(s) 1606
Eco130I CCWWGG 4 cut(s) 315, 1800, 2603, 2609
Eco24I GRGCYC 3 cut(s) 54, 147, 988
Eco32I GATATC 2 cut(s) 1899, 2131
Eco47I GGWCC 4 cut(s) 594, 657, 891, 1179
Eco47III AGCGCT 1 cut(s) 1729
Eco52I CGGCCG 1 cut(s) 1606
Eco53kI GAGCTC 2 cut(s) 52, 145
Eco57I CTGAAG 4 cut(s) 324, 408, 798, 1833
Eco72I CACGTG 1 cut(s) 863
Eco88I CYCGRG 1 cut(s) 65
EcoICRI GAGCTC 2 cut(s) 52, 145
EcoNI CCTNNNNNAGG 1 cut(s) 1071
EcoO109I RGGNCCY 5 cut(s) 498, 594, 657, 891, 1179
EcoRI GAATTC 1 cut(s) 472
EcoRII CCWGG 3 cut(s) 991, 2064, 2762
EcoRV GATATC 2 cut(s) 1899, 2131
EcoT14I CCWWGG 4 cut(s) 315, 1800, 2603, 2609
EcoT22I ATGCAT 1 cut(s) 2623
EcoT38I GRGCYC 3 cut(s) 54, 147, 988
EgeI GGCGCC 2 cut(s) 77, 213
EheI GGCGCC 2 cut(s) 77, 213
ErhI CCWWGG 4 cut(s) 315, 1800, 2603, 2609
Esp3I CGTCTC 1 cut(s) 36
FalI AAGNNNNNCTT 6 cut(s) 1304, 1336, 2351, 2383, 2412, 2444
FaqI GGGAC 3 cut(s) 701, 1192, 2295
FauNDI CATATG 3 cut(s) 1629, 1652, 1672
FbaI TGATCA 1 cut(s) 1552
FblI GTMKAC 1 cut(s) 2307
FokI GGATG 4 cut(s) 248, 298, 1362, 1700
FriOI GRGCYC 3 cut(s) 54, 147, 988
FseI GGCCGGCC 1 cut(s) 2537
GlaI GCGC 6 cut(s) 77, 213, 369, 1729, 2483, 2499
GsaI CCCAGC 1 cut(s) 722
GsuI CTGGAG 2 cut(s) 633, 2003
HaeII RGCGCY 3 cut(s) 79, 215, 1731
HapII CCGG 4 cut(s) 215, 1420, 1605, 2534
HhaI GCGC 6 cut(s) 78, 214, 370, 1730, 2484, 2500
Hin1I GRCGYC 2 cut(s) 76, 212
Hin6I GCGC 6 cut(s) 76, 212, 368, 1728, 2482, 2498
HinP1I GCGC 6 cut(s) 76, 212, 368, 1728, 2482, 2498
HincII GTYRAC 1 cut(s) 1507
HindII GTYRAC 1 cut(s) 1507
HindIII AAGCTT 2 cut(s) 10, 2517
HinfI GANTC 5 cut(s) 374, 1255, 1460, 1853, 2741
HpaII CCGG 4 cut(s) 215, 1420, 1605, 2534
HphI GGTGA 4 cut(s) 495, 1056, 1949, 2384
Hpy166II GTNNAC 7 cut(s) 779, 1064, 1204, 1411, 1507, 2122, 2308
Hpy8I GTNNAC 7 cut(s) 779, 1064, 1204, 1411, 1507, 2122, 2308
Hpy99I CGWCG 1 cut(s) 143
HpyCH4III ACNGT 7 cut(s) 682, 789, 880, 1151, 1415, 1994, 2248
HpyCH4IV ACGT 2 cut(s) 862, 2686
HpyF3I CTNAG 9 cut(s) 444, 633, 726, 904, 1248, 1288, 2225, 2472, 2667
HpySE526I ACGT 2 cut(s) 862, 2686
Hsp92I GRCGYC 2 cut(s) 76, 212
HspAI GCGC 6 cut(s) 76, 212, 368, 1728, 2482, 2498
KasI GGCGCC 2 cut(s) 75, 211
KflI GGGWCCC 1 cut(s) 1179
Kpn2I TCCGGA 1 cut(s) 1419
KroI GCCGGC 2 cut(s) 1604, 2533
KroNI GCCGGC 2 cut(s) 1606, 2535
Ksp22I TGATCA 1 cut(s) 1552
LguI GCTCTTC 1 cut(s) 42
LweI GCATC 7 cut(s) 60, 139, 449, 919, 2146, 2533, 2630
MaeII ACGT 2 cut(s) 862, 2686
MaeIII GTNAC 5 cut(s) 488, 1123, 2215, 2372, 2713
MbiI CCGCTC 1 cut(s) 220
MfeI CAATTG 1 cut(s) 1755
MflI RGATCY 3 cut(s) 536, 674, 707
MhlI GDGCHC 6 cut(s) 54, 147, 988, 1000, 1754, 2124
Mly113I GGCGCC 2 cut(s) 76, 212
MlyI GAGTC 1 cut(s) 1847
MmeI TCCRAC 4 cut(s) 146, 899, 1003, 2230
Mph1103I ATGCAT 1 cut(s) 2623
MroI TCCGGA 1 cut(s) 1419
MroNI GCCGGC 2 cut(s) 1604, 2533
MroXI GAANNNNTTC 3 cut(s) 1081, 1306, 1319
MseI TTAA 4 cut(s) 198, 281, 1811, 2490
MslI CAYNNNNRTG 2 cut(s) 1560, 2634
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 4 cut(s) 215, 1420, 1605, 2534
MspR9I CCNGG 3 cut(s) 993, 2066, 2764
MunI CAATTG 1 cut(s) 1755
MvaI CCWGG 3 cut(s) 993, 2066, 2764
MvnI CGCG 2 cut(s) 81, 370
NaeI GCCGGC 2 cut(s) 1606, 2535
NarI GGCGCC 2 cut(s) 76, 212
NdeI CATATG 3 cut(s) 1629, 1652, 1672
NgoMIV GCCGGC 2 cut(s) 1604, 2533
NheI GCTAGC 3 cut(s) 397, 1487, 2138
NmuCI GTSAC 3 cut(s) 1123, 2215, 2372
NsiI ATGCAT 1 cut(s) 2623
NspI RCATGY 2 cut(s) 1479, 1590
NspV TTCGAA 1 cut(s) 842
PaeI GCATGC 1 cut(s) 1590
PaeR7I CTCGAG 1 cut(s) 65
PagI TCATGA 1 cut(s) 2095
PaqCI CACCTGC 2 cut(s) 301, 790
PciSI GCTCTTC 1 cut(s) 42
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 138
PdiI GCCGGC 2 cut(s) 1606, 2535
PdmI GAANNNNTTC 3 cut(s) 1081, 1306, 1319
PfeI GAWTC 4 cut(s) 374, 1255, 1460, 2741
PflMI CCANNNNNTGG 1 cut(s) 863
Ple19I CGATCG 1 cut(s) 470
PleI GAGTC 1 cut(s) 1847
PluTI GGCGCC 2 cut(s) 79, 215
PmaCI CACGTG 1 cut(s) 863
PmlI CACGTG 1 cut(s) 863
PpsI GAGTC 1 cut(s) 1847
Ppu21I YACGTR 1 cut(s) 863
PpuMI RGGWCCY 4 cut(s) 594, 657, 891, 1179
PshAI GACNNNNGTC 1 cut(s) 1653
PshBI ATTAAT 1 cut(s) 198
Psp124BI GAGCTC 2 cut(s) 54, 147
Psp5II RGGWCCY 4 cut(s) 594, 657, 891, 1179
Psp6I CCWGG 3 cut(s) 991, 2064, 2762
PspCI CACGTG 1 cut(s) 863
PspFI CCCAGC 1 cut(s) 718
PspGI CCWGG 3 cut(s) 991, 2064, 2762
PspPI GGNCC 8 cut(s) 498, 515, 558, 594, 657, 891, 1179, 2043
PspPPI RGGWCCY 4 cut(s) 594, 657, 891, 1179
PstNI CAGNNNCTG 3 cut(s) 2078, 2246, 2471
PsuI RGATCY 3 cut(s) 536, 674, 707
PvuI CGATCG 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 524
RigI GGCCGGCC 1 cut(s) 2537
RseI CAYNNNNRTG 2 cut(s) 1560, 2634
SacI GAGCTC 2 cut(s) 54, 147
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 4 cut(s) 198, 281, 1811, 2490
Sau96I GGNCC 8 cut(s) 498, 515, 558, 594, 657, 891, 1179, 2043
ScaI AGTACT 3 cut(s) 484, 1102, 2230
SchI GAGTC 1 cut(s) 1847
ScrFI CCNGG 3 cut(s) 993, 2066, 2764
SduI GDGCHC 6 cut(s) 54, 147, 988, 1000, 1754, 2124
SfaNI GCATC 7 cut(s) 60, 139, 449, 919, 2146, 2533, 2630
SfoI GGCGCC 2 cut(s) 77, 213
Sfr274I CTCGAG 1 cut(s) 65
SfuI TTCGAA 1 cut(s) 842
SinI GGWCC 4 cut(s) 594, 657, 891, 1179
SlaI CTCGAG 1 cut(s) 65
SmiMI CAYNNNNRTG 2 cut(s) 1560, 2634
SmlI CTYRAG 2 cut(s) 65, 2511
SmoI CTYRAG 2 cut(s) 65, 2511
SpeI ACTAGT 2 cut(s) 485, 2593
SphI GCATGC 1 cut(s) 1590
SsiI CCGC 5 cut(s) 73, 79, 135, 209, 220
SspDI GGCGCC 2 cut(s) 75, 211
SspI AATATT 2 cut(s) 1513, 1965
SstI GAGCTC 2 cut(s) 54, 147
StyD4I CCNGG 3 cut(s) 991, 2064, 2762
StyI CCWWGG 4 cut(s) 315, 1800, 2603, 2609
TaaI ACNGT 7 cut(s) 682, 789, 880, 1151, 1415, 1994, 2248
TaiI ACGT 2 cut(s) 865, 2689
TaqI TCGA 8 cut(s) 66, 147, 470, 842, 896, 1323, 1926, 2154
TatI WGTACW 8 cut(s) 189, 482, 776, 1100, 1168, 1410, 2228, 2507
TauI GCSGC 4 cut(s) 76, 81, 138, 212
TfiI GAWTC 4 cut(s) 374, 1255, 1460, 2741
Tru1I TTAA 4 cut(s) 198, 281, 1811, 2490
Tru9I TTAA 4 cut(s) 198, 281, 1811, 2490
TseFI GTSAC 3 cut(s) 1123, 2215, 2372
Tsp45I GTSAC 3 cut(s) 1123, 2215, 2372
TspDTI ATGAA 9 cut(s) 564, 759, 1715, 1887, 1989, 2084, 2476, 2555, 2754
TspGWI ACGGA 1 cut(s) 1472
Van91I CCANNNNNTGG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 2120
VpaK11BI GGWCC 4 cut(s) 594, 657, 891, 1179
VspI ATTAAT 1 cut(s) 198
XagI CCTNNNNNAGG 1 cut(s) 1071
XapI RAATTY 5 cut(s) 472, 940, 970, 1776, 1874
XbaI TCTAGA 4 cut(s) 671, 1070, 1856, 2035
XceI RCATGY 2 cut(s) 1479, 1590
XcmI CCANNNNNNNNNTGG 1 cut(s) 1741
XhoI CTCGAG 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 2307
XmnI GAANNNNTTC 3 cut(s) 1081, 1306, 1319
ZrmI AGTACT 3 cut(s) 484, 1102, 2230
Zsp2I ATGCAT 1 cut(s) 2623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.