Rh3AG121300

Flocculation protein FLO11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000913
Physical Location & Seq
Forward (+)
10408518 .. 10412426
3909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG121300.1

Sequence Viewer

Length: 1335 bp
ATGTTGTCCAGGCTAATTACTCCACCTGCTACCCCTCTATTTCCATCCTTGGAGATGGAACCGCAAAAGACTTCAGTTAGTCAGATTGAGGAACCTAATGCGCGTGATTCTGCTCCTCCAAAATCTAGGCTAGCAAACACAAAGTTAGAGGCTGCTTCAGACAGCAAAATAGCATCTAAGCAGTCAACATTGCCATCACGACCGAATTCTTCCAGTACTAGTAACAGGAGGCCCTCACCATCAAGAGGGCCAACAGCTGCTCCTCGTAGATCTGCAACACCAACTGGACGGCCCACCACATTACCTTCATCAACCAAGCCATCAAGGTCCTCCACACCCAATTCACGAACCACCTTACCATCTGCTAAGCCAGTGGCTCCTCCAGTGAGGTCCTCAACTCCTTCTAGATCCACCGTGCGTTCTTCTACACCAACTGGCAGATCTTCTGTCCCAGCTTCTAAGTCAACGTCAAGATCAGCAACACCAACTCGCCTACCAAGTTCATCAAGTACACCTGCACTGTCTGCTCCTCGTGGCCGTTCTTCTTCAGTATCAAAGTCAGCTCCCCCAAGTTCGAAAAATCCTGCGCCATCACGTGGAAGTTCTCCAACAGTAAAATCTAGGTCGAAATCCTCAGATATGCCTGCTTTCTCACTTGATGCTCCACCAAATTTGAGAACCACTTTGCCACAAAGGCCAAATTCAGCCTCTAGGGCTCGTCCTGGGGCACCAAGTGTTAGATCATCTTCTGTTGGAGCTGTTTCTAGTGGAAAACCTAGACAGCAATCATGTTCACCTTCTAGAGGAAGGGCTTCACATGGCACTGCCAGTACTAATGGGTACTCTCGTCAAGTCACAAGTAAATCATCTCCCAATGACAGTGATGATGTAAATCCTGTACTTATGGGGACCCAAAGTGTTGAGAGAGTAGTGAACCAGAGGAAATTGGCACCACCAAAGCAGTATGACCATTCTACTCAGAAGAATCCTGATGGGAAGTCTTTGTCCTCTGACAGCTTAGGCTTTGGAAGAACACTTTCAAAGAAATCCTTCGATATGGCTATGAGACACATGGATATAAGGCGAAGCGTGCAAGATAACCTACATCCCGTTCTGACAAAAGTTCCAGCCTCCTCTGTGTACAGTGTCCGGACAAGACCAACAAAAAGCAAGACTACTAATGCTACGGATTCTCCTCTTGCTACATGCAGCAATGCTAGCTCTGAACCAAGTGTCAACAATATTTCTGCATACTTTGAAGGTTGTGAAATTGAGGACATTGATCATGGAAGTGAGAAAGGAAACTCCTCTCCAGCATGCCAGCAAGGCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

46.66

Weight (kDa)

11.21

Isoelectric Point (pI)

82.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 34, 523, 1320
Acc36I ACCTGC 3 cut(s) 34, 523, 1320
AccB1I GGYRCC 2 cut(s) 727, 949
AccB7I CCANNNNNTGG 1 cut(s) 596
AccII CGCG 1 cut(s) 103
AccIII TCCGGA 1 cut(s) 1149
AciI CCGC 1 cut(s) 62
AclWI GGATC 1 cut(s) 402
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 3 cut(s) 205, 670, 700
AcuI CTGAAG 3 cut(s) 57, 141, 531
AcvI CACGTG 1 cut(s) 596
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 6 cut(s) 217, 511, 832, 842, 900, 1142
AfiI CCNNNNNNNGG 3 cut(s) 245, 596, 803
AgsI TTSAA 2 cut(s) 1041, 1259
AhlI ACTAGT 1 cut(s) 218
AjnI CCWGG 2 cut(s) 8, 721
AluBI AGCT 6 cut(s) 257, 455, 563, 758, 1017, 1221
AluI AGCT 6 cut(s) 257, 455, 563, 758, 1017, 1221
Alw26I GTCTC 1 cut(s) 1060
AlwI GGATC 1 cut(s) 402
Aor13HI TCCGGA 1 cut(s) 1149
AoxI GGCC 5 cut(s) 230, 248, 290, 535, 695
ApeKI GCWGC 3 cut(s) 152, 257, 1209
ApoI RAATTY 3 cut(s) 205, 670, 700
Asp700I GAANNNNTTC 3 cut(s) 811, 1036, 1049
AspLEI GCGC 2 cut(s) 103, 589
AspS9I GGNCC 6 cut(s) 231, 248, 291, 327, 390, 909
AsuHPI GGTGA 2 cut(s) 228, 786
AsuII TTCGAA 1 cut(s) 575
AsuNHI GCTAGC 2 cut(s) 130, 1217
AvaII GGWCC 3 cut(s) 327, 390, 909
BaeGI GKGCMC 1 cut(s) 730
BaeI ACNNNNGTAYC 2 cut(s) 832, 865
BanI GGYRCC 2 cut(s) 727, 949
BanII GRGCYC 1 cut(s) 718
BauI CACGAG 1 cut(s) 531
BbrPI CACGTG 1 cut(s) 596
BbvI GCAGC 3 cut(s) 139, 244, 1221
BccI CCATC 8 cut(s) 49, 52, 202, 247, 328, 367, 598, 986
BceAI ACGGC 2 cut(s) 305, 522
BciT130I CCWGG 2 cut(s) 10, 723
BclI TGATCA 1 cut(s) 1282
BcoDI GTCTC 1 cut(s) 1060
BcuI ACTAGT 1 cut(s) 218
BfuAI ACCTGC 3 cut(s) 34, 523, 1320
BglI GCCNNNNNGGC 3 cut(s) 694, 713, 1326
BglII AGATCT 2 cut(s) 269, 440
BisI GCNGC 3 cut(s) 153, 258, 1210
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 3 cut(s) 154, 259, 1211
BmcAI AGTACT 2 cut(s) 217, 832
Bme1390I CCNGG 2 cut(s) 10, 723
Bme18I GGWCC 3 cut(s) 327, 390, 909
BmgT120I GGNCC 6 cut(s) 231, 248, 291, 327, 390, 909
BmiI GGNNCC 7 cut(s) 60, 93, 378, 729, 910, 911, 951
BmrFI CCNGG 2 cut(s) 10, 723
BmsI GCATC 2 cut(s) 182, 649
BmtI GCTAGC 2 cut(s) 134, 1221
BpmI CTGGAG 2 cut(s) 366, 1296
Bpu10I CCTNAGC 1 cut(s) 1018
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 1 cut(s) 575
BsaAI YACGTR 1 cut(s) 596
BsaBI GATNNNNATC 1 cut(s) 891
BsaJI CCNNGG 2 cut(s) 48, 722
BsaWI WCCGGW 1 cut(s) 1149
BsaXI ACNNNNNCTCC 4 cut(s) 244, 274, 1177, 1207
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 596, 803
Bse1I ACTGG 6 cut(s) 213, 289, 371, 383, 439, 828
Bse3DI GCAATG 2 cut(s) 188, 1219
Bse8I GATNNNNATC 1 cut(s) 891
BseAI TCCGGA 1 cut(s) 1149
BseBI CCWGG 2 cut(s) 10, 723
BseDI CCNNGG 2 cut(s) 48, 722
BseGI GGATG 2 cut(s) 44, 1105
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 3 cut(s) 245, 596, 803
BseMI GCAATG 2 cut(s) 188, 1219
BseMII CTCAG 2 cut(s) 648, 992
BseNI ACTGG 6 cut(s) 213, 289, 371, 383, 439, 828
BseRI GAGGAG 7 cut(s) 105, 252, 369, 519, 1123, 1185, 1297
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 3 cut(s) 139, 244, 1221
BseYI CCCAGC 1 cut(s) 451
BsgI GTGCAG 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 103
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 5 cut(s) 232, 250, 292, 537, 697
BshNI GGYRCC 2 cut(s) 727, 949
BsiEI CGRYCG 1 cut(s) 203
BsiSI CCGG 1 cut(s) 1150
BslFI GGGAC 2 cut(s) 434, 922
BslI CCNNNNNNNGG 3 cut(s) 245, 596, 803
BsmAI GTCTC 1 cut(s) 1060
BsmFI GGGAC 2 cut(s) 434, 922
BsnI GGCC 5 cut(s) 232, 250, 292, 537, 697
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 2 cut(s) 718, 730
Bsp13I TCCGGA 1 cut(s) 1149
Bsp1407I TGTACA 1 cut(s) 1140
Bsp143I GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 1 cut(s) 62
BspANI GGCC 5 cut(s) 232, 250, 292, 537, 697
BspCNI CTCAG 2 cut(s) 647, 991
BspEI TCCGGA 1 cut(s) 1149
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 7 cut(s) 60, 93, 378, 729, 910, 911, 951
BspMI ACCTGC 3 cut(s) 34, 523, 1320
BspOI GCTAGC 2 cut(s) 134, 1221
BspPI GGATC 1 cut(s) 402
BspT104I TTCGAA 1 cut(s) 575
BspT107I GGYRCC 2 cut(s) 727, 949
BsrDI GCAATG 2 cut(s) 188, 1219
BsrGI TGTACA 1 cut(s) 1140
BsrI ACTGG 6 cut(s) 213, 289, 371, 383, 439, 828
BssECI CCNNGG 2 cut(s) 48, 722
BssMI GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
BssSI CACGAG 1 cut(s) 531
BssT1I CCWWGG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 531
Bst2UI CCWGG 2 cut(s) 10, 723
Bst4CI ACNGT 5 cut(s) 415, 522, 613, 881, 1145
BstAPI GCANNNNNTGC 1 cut(s) 524
BstAUI TGTACA 1 cut(s) 1140
BstBAI YACGTR 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 6 cut(s) 132, 645, 1091, 1219, 1318, 1322
BstDEI CTNAG 6 cut(s) 177, 366, 459, 634, 978, 1018
BstENI CCTNNNNNAGG 1 cut(s) 801
BstF5I GGATG 2 cut(s) 44, 1105
BstFNI CGCG 1 cut(s) 103
BstHHI GCGC 2 cut(s) 103, 589
BstKTI GATC 6 cut(s) 272, 410, 443, 476, 743, 1285
BstMAI GTCTC 1 cut(s) 1060
BstMBI GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 6 cut(s) 524, 694, 713, 1090, 1218, 1326
BstNI CCWGG 2 cut(s) 10, 723
BstNSI RCATGY 2 cut(s) 1209, 1320
BstSCI CCNGG 2 cut(s) 8, 721
BstSLI GKGCMC 1 cut(s) 730
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 3 cut(s) 139, 244, 1221
BstX2I RGATCY 3 cut(s) 269, 407, 440
BstYI RGATCY 3 cut(s) 269, 407, 440
BsuRI GGCC 5 cut(s) 232, 250, 292, 537, 697
BtsCI GGATG 2 cut(s) 44, 1105
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 6 cut(s) 378, 390, 518, 822, 886, 1150
BveI ACCTGC 3 cut(s) 34, 523, 1320
Cac8I GCNNGC 6 cut(s) 132, 645, 1091, 1219, 1318, 1322
CfoI GCGC 2 cut(s) 103, 589
Cfr13I GGNCC 6 cut(s) 231, 248, 291, 327, 390, 909
Csp6I GTAC 6 cut(s) 216, 510, 831, 841, 899, 1141
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviAII CATG 6 cut(s) 789, 818, 1072, 1206, 1286, 1317
CviQI GTAC 6 cut(s) 216, 510, 831, 841, 899, 1141
DdeI CTNAG 6 cut(s) 177, 366, 459, 634, 978, 1018
DpnI GATC 6 cut(s) 271, 409, 442, 475, 742, 1284
DpnII GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
DraIII CACNNNGTG 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 535
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 718
Eco47I GGWCC 3 cut(s) 327, 390, 909
Eco57I CTGAAG 3 cut(s) 57, 141, 531
Eco72I CACGTG 1 cut(s) 596
EcoNI CCTNNNNNAGG 1 cut(s) 801
EcoO109I RGGNCCY 4 cut(s) 231, 327, 390, 909
EcoRI GAATTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 8, 721
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 6 cut(s) 792, 821, 1075, 1209, 1289, 1320
FalI AAGNNNNNCTT 2 cut(s) 1034, 1066
FaqI GGGAC 2 cut(s) 434, 922
FatI CATG 6 cut(s) 788, 817, 1071, 1205, 1285, 1316
FbaI TGATCA 1 cut(s) 1282
Fnu4HI GCNGC 3 cut(s) 153, 258, 1210
FokI GGATG 2 cut(s) 31, 1092
FriOI GRGCYC 1 cut(s) 718
Fsp4HI GCNGC 3 cut(s) 153, 258, 1210
GlaI GCGC 2 cut(s) 102, 588
GluI GCNGC 3 cut(s) 153, 258, 1210
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 2 cut(s) 366, 1296
HaeIII GGCC 5 cut(s) 232, 250, 292, 537, 697
HapII CCGG 1 cut(s) 1150
HhaI GCGC 2 cut(s) 103, 589
Hin1II CATG 6 cut(s) 792, 821, 1075, 1209, 1289, 1320
Hin6I GCGC 2 cut(s) 101, 587
HinP1I GCGC 2 cut(s) 101, 587
HincII GTYRAC 3 cut(s) 186, 465, 1237
HindII GTYRAC 3 cut(s) 186, 465, 1237
HinfI GANTC 3 cut(s) 107, 985, 1190
HpaII CCGG 1 cut(s) 1150
HphI GGTGA 2 cut(s) 228, 786
Hpy166II GTNNAC 7 cut(s) 186, 465, 512, 794, 934, 1141, 1237
Hpy188I TCNGA 7 cut(s) 84, 160, 637, 981, 1012, 1116, 1225
Hpy188III TCNNGA 8 cut(s) 198, 243, 345, 405, 471, 801, 989, 1150
Hpy8I GTNNAC 7 cut(s) 186, 465, 512, 794, 934, 1141, 1237
HpyAV CCTTC 6 cut(s) 315, 411, 801, 807, 1060, 1253
HpyCH4III ACNGT 5 cut(s) 415, 522, 613, 881, 1145
HpyCH4IV ACGT 2 cut(s) 467, 595
HpyCH4V TGCA 5 cut(s) 275, 518, 1093, 1209, 1250
HpyF10VI GCNNNNNNNGC 6 cut(s) 524, 694, 713, 1090, 1218, 1326
HpyF3I CTNAG 6 cut(s) 177, 366, 459, 634, 978, 1018
HpySE526I ACGT 2 cut(s) 467, 595
Hsp92II CATG 6 cut(s) 792, 821, 1075, 1209, 1289, 1320
HspAI GCGC 2 cut(s) 101, 587
KflI GGGWCCC 1 cut(s) 909
Kpn2I TCCGGA 1 cut(s) 1149
Ksp22I TGATCA 1 cut(s) 1282
Kzo9I GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
LmnI GCTCC 7 cut(s) 118, 265, 382, 532, 568, 667, 755
Lsp1109I GCAGC 3 cut(s) 139, 244, 1221
LweI GCATC 2 cut(s) 182, 649
MaeII ACGT 2 cut(s) 467, 595
MaeIII GTNAC 2 cut(s) 221, 853
MalI GATC 6 cut(s) 271, 409, 442, 475, 742, 1284
MboI GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
MboII GAAGA 8 cut(s) 201, 414, 435, 534, 537, 738, 994, 1041
MflI RGATCY 3 cut(s) 269, 407, 440
MhlI GDGCHC 2 cut(s) 718, 730
MluCI AATT 7 cut(s) 15, 205, 340, 670, 700, 944, 1269
MmeI TCCRAC 2 cut(s) 632, 733
MroI TCCGGA 1 cut(s) 1149
MroXI GAANNNNTTC 3 cut(s) 811, 1036, 1049
MslI CAYNNNNRTG 1 cut(s) 1290
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 1150
MspR9I CCNGG 2 cut(s) 10, 723
MvaI CCWGG 2 cut(s) 10, 723
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 6 cut(s) 524, 694, 713, 1090, 1218, 1326
NdeII GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
NheI GCTAGC 2 cut(s) 130, 1217
NlaIII CATG 6 cut(s) 792, 821, 1075, 1209, 1289, 1320
NlaIV GGNNCC 7 cut(s) 60, 93, 378, 729, 910, 911, 951
NmuCI GTSAC 1 cut(s) 853
NspI RCATGY 2 cut(s) 1209, 1320
NspV TTCGAA 1 cut(s) 575
PaeI GCATGC 1 cut(s) 1320
PaqCI CACCTGC 3 cut(s) 34, 523, 1320
PdmI GAANNNNTTC 3 cut(s) 811, 1036, 1049
PfeI GAWTC 3 cut(s) 107, 985, 1190
PflMI CCANNNNNTGG 1 cut(s) 596
PkrI GCNGC 3 cut(s) 154, 259, 1211
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
Ppu21I YACGTR 1 cut(s) 596
PpuMI RGGWCCY 3 cut(s) 327, 390, 909
Psp5II RGGWCCY 3 cut(s) 327, 390, 909
Psp6I CCWGG 2 cut(s) 8, 721
PspCI CACGTG 1 cut(s) 596
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 2 cut(s) 8, 721
PspN4I GGNNCC 7 cut(s) 60, 93, 378, 729, 910, 911, 951
PspPI GGNCC 6 cut(s) 231, 248, 291, 327, 390, 909
PspPPI RGGWCCY 3 cut(s) 327, 390, 909
PsuI RGATCY 3 cut(s) 269, 407, 440
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 6 cut(s) 217, 511, 832, 842, 900, 1142
RsaNI GTAC 6 cut(s) 216, 510, 831, 841, 899, 1141
RseI CAYNNNNRTG 1 cut(s) 1290
SatI GCNGC 3 cut(s) 153, 258, 1210
Sau3AI GATC 6 cut(s) 269, 407, 440, 473, 740, 1282
Sau96I GGNCC 6 cut(s) 231, 248, 291, 327, 390, 909
ScaI AGTACT 2 cut(s) 217, 832
ScrFI CCNGG 2 cut(s) 10, 723
SduI GDGCHC 2 cut(s) 718, 730
SfaNI GCATC 2 cut(s) 182, 649
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 3 cut(s) 327, 390, 909
SmiMI CAYNNNNRTG 1 cut(s) 1290
SpeI ACTAGT 1 cut(s) 218
SphI GCATGC 1 cut(s) 1320
Sse9I AATT 7 cut(s) 15, 205, 340, 670, 700, 944, 1269
SsiI CCGC 1 cut(s) 62
SspI AATATT 1 cut(s) 1243
StyD4I CCNGG 2 cut(s) 8, 721
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 5 cut(s) 415, 522, 613, 881, 1145
TaiI ACGT 2 cut(s) 470, 598
TaqI TCGA 3 cut(s) 575, 626, 1053
TaqII GACCGA 1 cut(s) 217
TasI AATT 7 cut(s) 15, 205, 340, 670, 700, 944, 1269
TatI WGTACW 5 cut(s) 215, 509, 830, 898, 1140
TfiI GAWTC 3 cut(s) 107, 985, 1190
TscAI CASTG 6 cut(s) 378, 390, 525, 829, 886, 1150
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 3 cut(s) 152, 257, 1209
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 2 cut(s) 297, 492
TspGWI ACGGA 1 cut(s) 1202
TspRI CASTG 6 cut(s) 378, 390, 525, 829, 886, 1150
Van91I CCANNNNNTGG 1 cut(s) 596
VpaK11BI GGWCC 3 cut(s) 327, 390, 909
XagI CCTNNNNNAGG 1 cut(s) 801
XapI RAATTY 3 cut(s) 205, 670, 700
XbaI TCTAGA 2 cut(s) 404, 800
XceI RCATGY 2 cut(s) 1209, 1320
XmnI GAANNNNTTC 3 cut(s) 811, 1036, 1049
ZrmI AGTACT 2 cut(s) 217, 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.