RLG00000024794

Flocculation protein FLO11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000913
Physical Location & Seq
Reverse (-)
39166732 .. 39173920
7189 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024794

Sequence Viewer

Length: 2640 bp
ATGGAAAGAAGCTTCGGGAAGGTGGTCAATGCCAACGACAGAGACGAAGAGCTCGCTCTGTTTCTCGAGATGCGGCGCCGCGACAAGGATAAGGAGAAAAATGGGAACTTTCTGCTGCTTCCGACCAAGAACGGCGGCGACGAGCTTGATGCTGCTGCTCCGATTGAGTATGATAAGGCGATTTACAAGATTAATGGGTCGGCCGCGCCGGAGCGGAGGAGTAGAGTGGATGAGTTTTTGAACTCGGAGAATGAGAAATCGGACTACGATTGGCTAATTACTCCACCTGCTACCCCACTATTTCCATCCTTGGAGATGGAACCACAAAAGACTTCGGTTAGTCAGATTGAGGAACCTAATGCGCGTGATTCTGCTCCTCCAAAATCTAGGCTAGCAAACACAAAGTTAGAAACTGCTTCAGACAGCAAAATAGCATCTAAGCAGTCAACATTGCCATCACGACCGAATTCTTCCAGTACTAGTAACAGGAGGCCCTCACCATCAAGAGGGCCAACAGCTGCTCCTCGTAGATCTGCAACACCAACTGGACGGCCCACCACATTACCTTCATCAACCAAGCCATCAAGGTCCTCCACACCCAATTCACGAACCACCTTACCATCTGCTAAGCCAGTGGCTCCTCCAGTGAGGTCCTCAACTCCTTCTAGATCCACCGTGCGTTCTTCTACACCAACTGGCAGATCTTCTGTCCCAGCTTCTAAGACAACATCAAGATCAGCAACACCAACTCGCATACCAAGTTCATCAAGTACACCTGCACTGTCTGCTCCTCGTGGCCGTTCTTCTTCAGTATCAAAGTCAGCTCCCCCAAGTTCAAAAAATCCTGTGCCATCACGTGGAAGTTCTCCAACAGTAAAATCTAGGTCCTCGAAATCCTCAGATATGCCTGCTTTCTCACTTGATGCTCCACCAAATTTGAGAACCACTTTGCCACAAAGGCCAAATTCAGCCTCTAGGGCTCGTCCTGGGGCACCAAGTGTTAGATCATCTTCTGTTGGAGCTGTTTCTAGTGGAAAACCTAGACAGCAATCATGTTCACCTTCTAGAGGAAGGGCTTCACATGGCACTGCCAGTACTAATGGGTACTCTCGTCAAGTCACAAGTAAATCATCTCCCAATGACAGTGATGATGTAAATCCTGTACTTATGGGGACCCAAAGTGTTGAGAGAGTAGTGAACCAGAGGAAACTGGCACCACCAAAGCAGTATGACCATTCTACTCAGAAGAATCCCGATGGGAAGTCTTTGTCCTCTGACAGCTTAGGCTTTGGAAGAACACTTTCAAAGAAATCCTTCGATATGGCTATGAGACACATGGATATAAGGCGAAGCGTGCAAGATAACCTACATCCTGTTCTGACAAAAGTTCCAGCCTCCTCTGTGTACAGTGTCCGGACAAGACCAACAAAAAGCAAGACTACAAATGCTACGGATTCTCCTCTTGCCACATGCAGCAATGCTAGCTCTGAACCAAGTGTCAATAATATTTCTGCATACTTTGAAGGTTGTGAAATTGAGGACATTGATGATGGAAGTGAGAAAGGAAACTCCTCTCCTGCATGCCAGCAAGGCAGTGGGTTGCTGAATTTCAAGGTTCGGGGTATAAGAGGTTTAAGAGGAAAGACGAATTTGGAACCTTCCAGCCAAGCCCAAGGGATTCCTACCAACAATGTAAGGCTCTTTTCTTATAATTCATTGAGGTCAGCGACCAGGAATTTTCATCCTTCAAACAAAATAGGTGGAGGAGGCTATGGAGTTGTCTATAAGGGAGTTTTAAGAGATGACACAGAAGTTGCGATCAAGTGTCTTTCTTCAGACTCTAGACAAGGAGCAAATGAATTTTTGGCAGAGATCAACACGATATCAAGAATACGACACCCAAACCTTGTTGAGCTTATTGGCTGCTGTGTGGAGGACAATCACCGAATATTGGTATATGAATATCTGGAGAACAACAGTCTTGCAAGTTCTTTGCTTGGTTCAAAAAGTCATGTTCCTCTAGATTGGCCCCAGAGAGCAGCAATTTGCCTGGATTTTGGGCTGGCAAAACTTTTTCCTGATAATGTCACTCATCTTAGTACTAGAGTGGCAGGAACTGTTGGATATCTAGCCCCAGAATATGCACTTTTGGGACAGCTTACAAAAAAAGCAGATGTATACAGCTTTGGGGTTCTCTTGCTTGAAATAATTAGTGGCAGAAGTAGTAGTAAGGCTGCCTTTGGTGACGAACTGATGCTTCTGGTGGAATGGACTTGGAAACTGAAAGAAGAAGAAAGGCTTATAGAAATTGTTGATCCAGAATTGACTGCATATCCAGAGGCTGAGGTGATGCGCTTCATTAAGGTTGCGCTCTTTTGTACTCAAGAAGCTTCGCATCAAAGGCCGGCCATGAAGCAAGTAGTGGAAATGCTTTCCAAAGAGGTACAGCTAAATGAGAAGCTACTAGTAGAACCAAGGCCAAGGAGTTATGCACCTCAACATGGAGGTGGTGCCAGTTGGGAGGGAACATCGTCTTCTCAGTCAGATAAAGATAAACGCGGGAAATCATTGGCAAATCCTGACGTAACTTCCACCCAGTCGTATATACATGAATCTCAAACGCAAATGTTTCCCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

880

Amino Acids

95.25

Weight (kDa)

9.65

Isoelectric Point (pI)

60.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 581 - 665 2.3e-14 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 583 - 675 2.3e-16 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 684 - 809 3.2e-09 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 685 - 811 8.8e-11 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1710
AarI CACCTGC 2 cut(s) 295, 784
Acc36I ACCTGC 2 cut(s) 295, 784
AccB1I GGYRCC 4 cut(s) 75, 991, 1213, 2510
AccB7I CCANNNNNTGG 1 cut(s) 857
AccBSI CCGCTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 2178
AccII CGCG 4 cut(s) 81, 206, 364, 2559
AccIII TCCGGA 1 cut(s) 1413
AciI CCGC 6 cut(s) 73, 79, 135, 204, 214, 2559
AclWI GGATC 2 cut(s) 663, 2309
AcoI YGGCCR 3 cut(s) 201, 796, 2406
AcsI RAATTY 7 cut(s) 466, 934, 964, 1606, 1648, 1735, 1859
AcuI CTGAAG 3 cut(s) 402, 792, 1818
AcvI CACGTG 1 cut(s) 857
AcyI GRCGYC 1 cut(s) 76
AdeI CACNNNGTG 1 cut(s) 998
AfaI GTAC 9 cut(s) 478, 772, 1096, 1106, 1164, 1406, 2101, 2380, 2445
AfiI CCNNNNNNNGG 6 cut(s) 85, 506, 857, 1067, 1951, 2501
AgsI TTSAA 8 cut(s) 241, 837, 1305, 1523, 1612, 1749, 2004, 2204
AhlI ACTAGT 2 cut(s) 479, 2464
AjnI CCWGG 4 cut(s) 985, 1730, 2049, 2633
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 2 cut(s) 36, 1324
AlwI GGATC 2 cut(s) 663, 2309
AlwNI CAGNNNCTG 2 cut(s) 2117, 2342
Ama87I CYCGRG 1 cut(s) 65
Aor13HI TCCGGA 1 cut(s) 1413
ApeKI GCWGC 8 cut(s) 115, 152, 155, 518, 1473, 1923, 2039, 2234
ApoI RAATTY 7 cut(s) 466, 934, 964, 1606, 1648, 1735, 1859
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 3 cut(s) 1075, 1300, 1313
AspLEI GCGC 5 cut(s) 78, 208, 364, 2355, 2371
AspS9I GGNCC 8 cut(s) 492, 509, 552, 588, 651, 885, 1173, 2028
AsuHPI GGTGA 5 cut(s) 489, 1050, 1934, 2255, 2359
AsuNHI GCTAGC 2 cut(s) 391, 1481
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 4 cut(s) 588, 651, 885, 1173
BaeGI GKGCMC 1 cut(s) 994
BaeI ACNNNNGTAYC 2 cut(s) 1096, 1129
BanI GGYRCC 4 cut(s) 75, 991, 1213, 2510
BanII GRGCYC 2 cut(s) 54, 982
BauI CACGAG 1 cut(s) 792
BbrPI CACGTG 1 cut(s) 857
BbsI GAAGAC 1 cut(s) 2526
Bbv12I GWGCWC 1 cut(s) 54
BbvCI CCTCAGC 1 cut(s) 2343
BbvI GCAGC 8 cut(s) 102, 139, 142, 505, 1485, 1910, 2051, 2221
BccI CCATC 9 cut(s) 310, 313, 463, 508, 589, 628, 859, 1250, 1544
BceAI ACGGC 3 cut(s) 148, 566, 783
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 4 cut(s) 987, 1732, 2051, 2635
BcoDI GTCTC 2 cut(s) 36, 1324
BcuI ACTAGT 2 cut(s) 479, 2464
BfoI RGCGCY 1 cut(s) 79
BfuAI ACCTGC 2 cut(s) 295, 784
BglI GCCNNNNNGGC 3 cut(s) 958, 977, 1590
BglII AGATCT 2 cut(s) 530, 701
BlpI GCTNAGC 1 cut(s) 627
BmcAI AGTACT 3 cut(s) 478, 1096, 2101
Bme1390I CCNGG 4 cut(s) 987, 1732, 2051, 2635
Bme18I GGWCC 4 cut(s) 588, 651, 885, 1173
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 8 cut(s) 492, 509, 552, 588, 651, 885, 1173, 2028
BmrFI CCNGG 4 cut(s) 987, 1732, 2051, 2635
BmrI ACTGGG 1 cut(s) 2590
BmsI GCATC 7 cut(s) 60, 139, 443, 913, 2244, 2340, 2404
BmtI GCTAGC 2 cut(s) 395, 1485
BmuI ACTGGG 1 cut(s) 2590
BpiI GAAGAC 1 cut(s) 2526
BpmI CTGGAG 2 cut(s) 627, 1988
Bpu10I CCTNAGC 2 cut(s) 1282, 2343
Bpu1102I GCTNAGC 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 2367
BsaAI YACGTR 1 cut(s) 857
BsaBI GATNNNNATC 1 cut(s) 1155
BsaHI GRCGYC 1 cut(s) 76
BsaJI CCNNGG 6 cut(s) 309, 986, 1672, 2474, 2480, 2633
BsaWI WCCGGW 1 cut(s) 1413
BsaXI ACNNNNNCTCC 4 cut(s) 505, 535, 1441, 1471
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 506, 857, 1067, 1951, 2501
Bse118I RCCGGY 1 cut(s) 2404
Bse1I ACTGG 9 cut(s) 474, 550, 632, 644, 700, 1092, 1215, 2514, 2596
Bse3DI GCAATG 2 cut(s) 449, 1483
Bse8I GATNNNNATC 1 cut(s) 1155
BseAI TCCGGA 1 cut(s) 1413
BseBI CCWGG 4 cut(s) 987, 1732, 2051, 2635
BseDI CCNNGG 6 cut(s) 309, 986, 1672, 2474, 2480, 2633
BseGI GGATG 4 cut(s) 235, 305, 1369, 1741
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 6 cut(s) 85, 506, 857, 1067, 1951, 2501
BseMI GCAATG 2 cut(s) 449, 1483
BseMII CTCAG 4 cut(s) 912, 1256, 2334, 2552
BseNI ACTGG 9 cut(s) 474, 550, 632, 644, 700, 1092, 1215, 2514, 2596
BseRI GAGGAG 9 cut(s) 232, 366, 513, 630, 780, 1387, 1449, 1561, 1779
BseSI GKGCMC 1 cut(s) 994
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 8 cut(s) 102, 139, 142, 505, 1485, 1910, 2051, 2221
BseYI CCCAGC 1 cut(s) 712
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 4 cut(s) 81, 206, 364, 2559
Bsh1285I CGRYCG 2 cut(s) 204, 464
BshNI GGYRCC 4 cut(s) 75, 991, 1213, 2510
BsiEI CGRYCG 2 cut(s) 204, 464
BsiHKAI GWGCWC 1 cut(s) 54
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 3 cut(s) 209, 1414, 2405
BslFI GGGAC 3 cut(s) 695, 1186, 2166
BslI CCNNNNNNNGG 6 cut(s) 85, 506, 857, 1067, 1951, 2501
BsmAI GTCTC 2 cut(s) 36, 1324
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 3 cut(s) 695, 1186, 2166
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 3 cut(s) 54, 982, 994
Bsp13I TCCGGA 1 cut(s) 1413
Bsp1407I TGTACA 1 cut(s) 1404
Bsp143I GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
Bsp1720I GCTNAGC 1 cut(s) 627
BspACI CCGC 6 cut(s) 73, 79, 135, 204, 214, 2559
BspCNI CTCAG 4 cut(s) 911, 1255, 2335, 2551
BspEI TCCGGA 1 cut(s) 1413
BspFNI CGCG 4 cut(s) 81, 206, 364, 2559
BspMI ACCTGC 2 cut(s) 295, 784
BspOI GCTAGC 2 cut(s) 395, 1485
BspPI GGATC 2 cut(s) 663, 2309
BspQI GCTCTTC 1 cut(s) 42
BspT107I GGYRCC 4 cut(s) 75, 991, 1213, 2510
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 449, 1483
BsrFI RCCGGY 1 cut(s) 2404
BsrGI TGTACA 1 cut(s) 1404
BsrI ACTGG 9 cut(s) 474, 550, 632, 644, 700, 1092, 1215, 2514, 2596
BssAI RCCGGY 1 cut(s) 2404
BssECI CCNNGG 6 cut(s) 309, 986, 1672, 2474, 2480, 2633
BssMI GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
BssNAI GTATAC 1 cut(s) 2179
BssNI GRCGYC 1 cut(s) 76
BssSI CACGAG 1 cut(s) 792
BssT1I CCWWGG 4 cut(s) 309, 1672, 2474, 2480
Bst1107I GTATAC 1 cut(s) 2179
Bst2BI CACGAG 1 cut(s) 792
Bst2UI CCWGG 4 cut(s) 987, 1732, 2051, 2635
Bst4CI ACNGT 7 cut(s) 676, 783, 874, 1145, 1409, 1979, 2119
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 76
BstAPI GCANNNNNTGC 1 cut(s) 785
BstAUI TGTACA 1 cut(s) 1404
BstBAI YACGTR 1 cut(s) 857
BstC8I GCNNGC 9 cut(s) 54, 393, 909, 1355, 1483, 1582, 1586, 2064, 2406
BstDEI CTNAG 9 cut(s) 438, 627, 720, 898, 1242, 1282, 2096, 2343, 2538
BstENI CCTNNNNNAGG 1 cut(s) 1065
BstF5I GGATG 4 cut(s) 235, 305, 1369, 1741
BstFNI CGCG 4 cut(s) 81, 206, 364, 2559
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 5 cut(s) 78, 208, 364, 2355, 2371
BstKTI GATC 8 cut(s) 533, 671, 704, 737, 1007, 1821, 1875, 2317
BstMAI GTCTC 2 cut(s) 36, 1324
BstMBI GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
BstMCI CGRYCG 2 cut(s) 204, 464
BstMWI GCNNNNNNNGC 7 cut(s) 785, 958, 977, 1354, 1482, 1590, 2401
BstNI CCWGG 4 cut(s) 987, 1732, 2051, 2635
BstNSI RCATGY 2 cut(s) 1473, 1584
BstSCI CCNGG 4 cut(s) 985, 1730, 2049, 2633
BstSLI GKGCMC 1 cut(s) 994
BstUI CGCG 4 cut(s) 81, 206, 364, 2559
BstV1I GCAGC 8 cut(s) 102, 139, 142, 505, 1485, 1910, 2051, 2221
BstV2I GAAGAC 1 cut(s) 2526
BstX2I RGATCY 3 cut(s) 530, 668, 701
BstYI RGATCY 3 cut(s) 530, 668, 701
BstZ17I GTATAC 1 cut(s) 2179
BstZI CGGCCG 1 cut(s) 201
BtsCI GGATG 4 cut(s) 235, 305, 1369, 1741
BtsI GCAGTG 2 cut(s) 1086, 1600
BtsIMutI CAGTG 7 cut(s) 639, 651, 779, 1086, 1150, 1414, 1600
BveI ACCTGC 2 cut(s) 295, 784
Cac8I GCNNGC 9 cut(s) 54, 393, 909, 1355, 1483, 1582, 1586, 2064, 2406
CaiI CAGNNNCTG 2 cut(s) 2117, 2342
CfoI GCGC 5 cut(s) 78, 208, 364, 2355, 2371
Cfr10I RCCGGY 1 cut(s) 2404
Cfr13I GGNCC 8 cut(s) 492, 509, 552, 588, 651, 885, 1173, 2028
Csp6I GTAC 9 cut(s) 477, 771, 1095, 1105, 1163, 1405, 2100, 2379, 2444
CspCI CAANNNNNGTGG 4 cut(s) 918, 953, 1741, 1776
CviAII CATG 9 cut(s) 1053, 1082, 1336, 1470, 1581, 2012, 2410, 2501, 2609
CviQI GTAC 9 cut(s) 477, 771, 1095, 1105, 1163, 1405, 2100, 2379, 2444
DdeI CTNAG 9 cut(s) 438, 627, 720, 898, 1242, 1282, 2096, 2343, 2538
DinI GGCGCC 1 cut(s) 77
DpnI GATC 8 cut(s) 532, 670, 703, 736, 1006, 1820, 1874, 2316
DpnII GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
DraIII CACNNNGTG 1 cut(s) 998
EaeI YGGCCR 3 cut(s) 201, 796, 2406
EagI CGGCCG 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 52
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 4 cut(s) 309, 1672, 2474, 2480
Eco24I GRGCYC 2 cut(s) 54, 982
Eco32I GATATC 2 cut(s) 1884, 2126
Eco47I GGWCC 4 cut(s) 588, 651, 885, 1173
Eco52I CGGCCG 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 3 cut(s) 402, 792, 1818
Eco72I CACGTG 1 cut(s) 857
Eco88I CYCGRG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 52
EcoNI CCTNNNNNAGG 1 cut(s) 1065
EcoO109I RGGNCCY 5 cut(s) 492, 588, 651, 885, 1173
EcoRI GAATTC 1 cut(s) 466
EcoRII CCWGG 4 cut(s) 985, 1730, 2049, 2633
EcoRV GATATC 2 cut(s) 1884, 2126
EcoT14I CCWWGG 4 cut(s) 309, 1672, 2474, 2480
EcoT38I GRGCYC 2 cut(s) 54, 982
EgeI GGCGCC 1 cut(s) 77
EheI GGCGCC 1 cut(s) 77
ErhI CCWWGG 4 cut(s) 309, 1672, 2474, 2480
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 9 cut(s) 1056, 1085, 1339, 1473, 1584, 2015, 2413, 2504, 2612
FalI AAGNNNNNCTT 6 cut(s) 1298, 1330, 2222, 2254, 2283, 2315
FaqI GGGAC 3 cut(s) 695, 1186, 2166
FatI CATG 9 cut(s) 1052, 1081, 1335, 1469, 1580, 2011, 2409, 2500, 2608
FauI CCCGC 1 cut(s) 2552
FblI GTMKAC 1 cut(s) 2178
FokI GGATG 4 cut(s) 242, 292, 1356, 1728
FriOI GRGCYC 2 cut(s) 54, 982
FseI GGCCGGCC 1 cut(s) 2408
GlaI GCGC 5 cut(s) 77, 207, 363, 2354, 2370
GsaI CCCAGC 1 cut(s) 716
GsuI CTGGAG 2 cut(s) 627, 1988
HaeII RGCGCY 1 cut(s) 79
HapII CCGG 3 cut(s) 209, 1414, 2405
HhaI GCGC 5 cut(s) 78, 208, 364, 2355, 2371
Hin1I GRCGYC 1 cut(s) 76
Hin1II CATG 9 cut(s) 1056, 1085, 1339, 1473, 1584, 2015, 2413, 2504, 2612
Hin6I GCGC 5 cut(s) 76, 206, 362, 2353, 2369
HinP1I GCGC 5 cut(s) 76, 206, 362, 2353, 2369
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HindIII AAGCTT 2 cut(s) 10, 2388
HinfI GANTC 6 cut(s) 368, 1249, 1454, 1678, 1838, 2612
HpaII CCGG 3 cut(s) 209, 1414, 2405
HphI GGTGA 5 cut(s) 489, 1050, 1934, 2255, 2359
Hpy166II GTNNAC 6 cut(s) 447, 773, 1058, 1198, 1405, 2179
Hpy8I GTNNAC 6 cut(s) 447, 773, 1058, 1198, 1405, 2179
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 9 cut(s) 13, 576, 672, 1065, 1071, 1324, 1517, 1668, 1755
HpyCH4III ACNGT 7 cut(s) 676, 783, 874, 1145, 1409, 1979, 2119
HpyCH4IV ACGT 2 cut(s) 856, 2583
HpyF10VI GCNNNNNNNGC 7 cut(s) 785, 958, 977, 1354, 1482, 1590, 2401
HpyF3I CTNAG 9 cut(s) 438, 627, 720, 898, 1242, 1282, 2096, 2343, 2538
HpySE526I ACGT 2 cut(s) 856, 2583
Hsp92I GRCGYC 1 cut(s) 76
Hsp92II CATG 9 cut(s) 1056, 1085, 1339, 1473, 1584, 2015, 2413, 2504, 2612
HspAI GCGC 5 cut(s) 76, 206, 362, 2353, 2369
KasI GGCGCC 1 cut(s) 75
KflI GGGWCCC 1 cut(s) 1173
Kpn2I TCCGGA 1 cut(s) 1413
KroI GCCGGC 1 cut(s) 2404
KroNI GCCGGC 1 cut(s) 2406
Kzo9I GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
LguI GCTCTTC 1 cut(s) 42
Lsp1109I GCAGC 8 cut(s) 102, 139, 142, 505, 1485, 1910, 2051, 2221
LweI GCATC 7 cut(s) 60, 139, 443, 913, 2244, 2340, 2404
MaeII ACGT 2 cut(s) 856, 2583
MaeIII GTNAC 5 cut(s) 482, 1117, 2086, 2243, 2584
MalI GATC 8 cut(s) 532, 670, 703, 736, 1006, 1820, 1874, 2316
MbiI CCGCTC 1 cut(s) 214
MboI GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
MflI RGATCY 3 cut(s) 530, 668, 701
MhlI GDGCHC 3 cut(s) 54, 982, 994
Mly113I GGCGCC 1 cut(s) 76
MlyI GAGTC 1 cut(s) 1832
MmeI TCCRAC 4 cut(s) 146, 893, 997, 2101
MroI TCCGGA 1 cut(s) 1413
MroNI GCCGGC 1 cut(s) 2404
MroXI GAANNNNTTC 3 cut(s) 1075, 1300, 1313
MseI TTAA 4 cut(s) 192, 1634, 1796, 2361
MslI CAYNNNNRTG 1 cut(s) 2505
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 3 cut(s) 209, 1414, 2405
MspR9I CCNGG 4 cut(s) 987, 1732, 2051, 2635
MvaI CCWGG 4 cut(s) 987, 1732, 2051, 2635
MvnI CGCG 4 cut(s) 81, 206, 364, 2559
MwoI GCNNNNNNNGC 7 cut(s) 785, 958, 977, 1354, 1482, 1590, 2401
NaeI GCCGGC 1 cut(s) 2406
NarI GGCGCC 1 cut(s) 76
NdeII GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
NgoMIV GCCGGC 1 cut(s) 2404
NheI GCTAGC 2 cut(s) 391, 1481
NlaIII CATG 9 cut(s) 1056, 1085, 1339, 1473, 1584, 2015, 2413, 2504, 2612
NmuCI GTSAC 3 cut(s) 1117, 2086, 2243
NspI RCATGY 2 cut(s) 1473, 1584
PaeI GCATGC 1 cut(s) 1584
PaeR7I CTCGAG 1 cut(s) 65
PaqCI CACCTGC 2 cut(s) 295, 784
PciSI GCTCTTC 1 cut(s) 42
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 138
PdiI GCCGGC 1 cut(s) 2406
PdmI GAANNNNTTC 3 cut(s) 1075, 1300, 1313
PfeI GAWTC 5 cut(s) 368, 1249, 1454, 1678, 2612
PflMI CCANNNNNTGG 1 cut(s) 857
PleI GAGTC 1 cut(s) 1832
PluTI GGCGCC 1 cut(s) 79
PmaCI CACGTG 1 cut(s) 857
PmlI CACGTG 1 cut(s) 857
PpsI GAGTC 1 cut(s) 1832
Ppu21I YACGTR 1 cut(s) 857
PpuMI RGGWCCY 4 cut(s) 588, 651, 885, 1173
PshBI ATTAAT 1 cut(s) 192
PsiI TTATAA 1 cut(s) 1710
Psp124BI GAGCTC 1 cut(s) 54
Psp5II RGGWCCY 4 cut(s) 588, 651, 885, 1173
Psp6I CCWGG 4 cut(s) 985, 1730, 2049, 2633
PspCI CACGTG 1 cut(s) 857
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 4 cut(s) 985, 1730, 2049, 2633
PspPI GGNCC 8 cut(s) 492, 509, 552, 588, 651, 885, 1173, 2028
PspPPI RGGWCCY 4 cut(s) 588, 651, 885, 1173
PstNI CAGNNNCTG 2 cut(s) 2117, 2342
PsuI RGATCY 3 cut(s) 530, 668, 701
PvuII CAGCTG 1 cut(s) 518
RigI GGCCGGCC 1 cut(s) 2408
RsaI GTAC 9 cut(s) 478, 772, 1096, 1106, 1164, 1406, 2101, 2380, 2445
RsaNI GTAC 9 cut(s) 477, 771, 1095, 1105, 1163, 1405, 2100, 2379, 2444
RseI CAYNNNNRTG 1 cut(s) 2505
SacI GAGCTC 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 4 cut(s) 192, 1634, 1796, 2361
Sau3AI GATC 8 cut(s) 530, 668, 701, 734, 1004, 1818, 1872, 2314
Sau96I GGNCC 8 cut(s) 492, 509, 552, 588, 651, 885, 1173, 2028
ScaI AGTACT 3 cut(s) 478, 1096, 2101
SchI GAGTC 1 cut(s) 1832
ScrFI CCNGG 4 cut(s) 987, 1732, 2051, 2635
SduI GDGCHC 3 cut(s) 54, 982, 994
SfaNI GCATC 7 cut(s) 60, 139, 443, 913, 2244, 2340, 2404
SfoI GGCGCC 1 cut(s) 77
Sfr274I CTCGAG 1 cut(s) 65
SinI GGWCC 4 cut(s) 588, 651, 885, 1173
SlaI CTCGAG 1 cut(s) 65
SmiMI CAYNNNNRTG 1 cut(s) 2505
SmlI CTYRAG 2 cut(s) 65, 2382
SmoI CTYRAG 2 cut(s) 65, 2382
SpeI ACTAGT 2 cut(s) 479, 2464
SphI GCATGC 1 cut(s) 1584
SsiI CCGC 6 cut(s) 73, 79, 135, 204, 214, 2559
SspDI GGCGCC 1 cut(s) 75
SspI AATATT 2 cut(s) 1507, 1950
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 4 cut(s) 985, 1730, 2049, 2633
StyI CCWWGG 4 cut(s) 309, 1672, 2474, 2480
TaaI ACNGT 7 cut(s) 676, 783, 874, 1145, 1409, 1979, 2119
TaiI ACGT 2 cut(s) 859, 2586
TaqI TCGA 3 cut(s) 66, 890, 1317
TaqII GACCGA 1 cut(s) 478
TatI WGTACW 7 cut(s) 476, 770, 1094, 1162, 1404, 2099, 2378
TauI GCSGC 4 cut(s) 76, 81, 138, 206
TfiI GAWTC 5 cut(s) 368, 1249, 1454, 1678, 2612
Tru1I TTAA 4 cut(s) 192, 1634, 1796, 2361
Tru9I TTAA 4 cut(s) 192, 1634, 1796, 2361
TscAI CASTG 7 cut(s) 639, 651, 786, 1093, 1150, 1414, 1600
TseFI GTSAC 3 cut(s) 1117, 2086, 2243
TseI GCWGC 8 cut(s) 115, 152, 155, 518, 1473, 1923, 2039, 2234
Tsp45I GTSAC 3 cut(s) 1117, 2086, 2243
TspDTI ATGAA 9 cut(s) 558, 753, 1704, 1730, 1872, 1974, 2347, 2426, 2625
TspGWI ACGGA 1 cut(s) 1466
TspRI CASTG 7 cut(s) 639, 651, 786, 1093, 1150, 1414, 1600
Van91I CCANNNNNTGG 1 cut(s) 857
VpaK11BI GGWCC 4 cut(s) 588, 651, 885, 1173
VspI ATTAAT 1 cut(s) 192
XagI CCTNNNNNAGG 1 cut(s) 1065
XapI RAATTY 7 cut(s) 466, 934, 964, 1606, 1648, 1735, 1859
XbaI TCTAGA 4 cut(s) 665, 1064, 1841, 2020
XceI RCATGY 2 cut(s) 1473, 1584
XcmI CCANNNNNNNNNTGG 1 cut(s) 1592
XhoI CTCGAG 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 2178
XmnI GAANNNNTTC 3 cut(s) 1075, 1300, 1313
ZrmI AGTACT 3 cut(s) 478, 1096, 2101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.