Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000913

Physical Location & Seq

Genomic Coordinates Forward (+)

44554851 .. 44558003

Gene Structure 3153 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1316300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGCGTTGCAGCTGCTTTGGTGCTTCGGTTGCTGAGAAGAGGAAAAGGAAATCTGGTTCTGGTCAATTTGGCCATGATCTTGATGAGAATGTGCTGGAAAATATCAAGGCTTTCTCTTACAATGAATTAAGATCTGCGACGGATAATTTCCATCCGAGTAACAAGATCGGGCGAGGCGGTTTTGGAACTGTGTACAAGGGAACCCTGACAAGTGGAAAGCATGTTGCTGTCAAGACACTTGCTGCTCAGTCGAAGCAGGGAGTCCGTGAATTCTTAAATGAGATTAACACTATCTCAAATGTCCGGCATCCAAACCTTGTTGAGTTGATTGGGTGCTGTGTTCAGGAAGCTGATCGAGTTTTGGTCTATGAATATGTTGAGAATAAAAGCCTTGATCATGCATTATTAGGTCCAACGAGTAGAACCATCAAACTGGATTGGGAAAAAAGATCAGCCATTTGCTTGGGTACTGCCAGGGGTCTTACGTATCTTCATGAAGAACTGGTGCCACATATTGTACATAGAGATATCAAGGCTAGTAATTTACTTTTAGACAGAGAATTTAACCCGAAAATCGGAGACTTTGGGTTGGCTAAACTCTTCCCTGATGATATCACTCATATCAGCACAAGGATTGCGGGCACAACTGGTTATTTGGCACCAGAATATGCATTGGGAGGTCAGTTAACCCTCAAGGCCGACGTTTACAGTTTTGGGGTTCTCGTTCTTGAAATAGTATGTGGAAGAAGCAGTGCCAAGGGAGACTGGGGTGGGGCAGAAAAGTTTCTGCTTGAATGGGCATGGCAGCTCCATGAAGAAGGGAGACTATTGGAGCTAGCTGACCCGGAGATGGGGGAATTTCCGGAGGAAGAAGTCCTTAGGTACATGAAGGTGGCATTTTTCTGCACCCAAGCAGCAGCAAACCGAAGACCAAAGATGAGCCAGGTTCTTGACATGCTCTCGAGAAACATAAGACTCAACGAGAAACAACTTACAGCCCCAGGTTTCTTCAACAGTTCAGGGAGCCTGAGCGGACCTTCAGGTAAAAAGTCATTTTCCGAAACCACTACCAGTCAGATGAGTTCCGTCCCCGACACTATAACTCAAGTAACACCTCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

374

Amino Acids

41.28

Weight (kDa)

7.63

Isoelectric Point (pI)

38.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	51 - 253	7.9e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	53 - 319	1.3e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000913)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09010 AT3G09010
fragaria_vesca	FvH4_3g04850 FvH4_3g04850 FvH4_3g04850 FvH4_3g04850 FvH4_3g04850 FvH4_3g04850 FvH4_6g11590 FvH4_6g11590 FvH4_6g11600 FvH4_6g11600 FvH4_6g11600 FvH4_6g11600
malus_domestica	MD04G1150800.v1.1 MD04G1150900.v1.1 MD05G1316300.v1.1 MD10G1296800.v1.1 MD12G1164800.v1.1
prunus_persica	Prupe.4G043800_v2.0.a1 Prupe.4G043800_v2.0.a1 Prupe.4G043800_v2.0.a1 Prupe.4G043800_v2.0.a1 Prupe.4G043800_v2.0.a1 Prupe.4G043800_v2.0.a1 Prupe.6G275600_v2.0.a1 Prupe.6G275600_v2.0.a1 Prupe.6G275700_v2.0.a1
pyrus_communis	pycom04g13650 pycom04g13660 pycom10g24970
rosa_chinensis	RchiOBHm_Chr3g0463271 RchiOBHm_Chr3g0463281 RchiOBHm_Chr5g0008011
rosa_laevigata	RLG00000024794 RLG00000031527
rosa_multiflora	Rmu_sc0002976.1_g000005 Rmu_sc0003073.1_g000002 Rmu_sc0007348.1_g000004 Rmu_sc0035275.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068460 Rroxscaffold_6G00416710
rosa_rugosa	Rorug03G0063300 Rorug03G0063300 Rorug03G0063400 Rorug03G0063400 Rorug03G0063400 Rorug03G0063500 Rorug04G0432400 Rorug04G0432500
rosa_samantha	Rh3AG121300 Rh3AG121400 Rh3BG125800 Rh3BG125900 Rh3CG127700 Rh3CG127800 Rh3DG126900 Rh3DG127000 Rh5AG060600 Rh5BG057400 Rh5CG067500 Rh5DG056600
rosa_wichuraiana	Rw3G010300 Rw3G010310 Rw5G006000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	505, 658
AccBSI	CCGCTC	1 cut(s)	1032
AccIII	TCCGGA	1 cut(s)	862
AciI	CCGC	3 cut(s)	177, 638, 1032
AcoI	YGGCCR	1 cut(s)	70
AcsI	RAATTY	3 cut(s)	269, 560, 857
AcuI	CTGAAG	1 cut(s)	1023
AfaI	GTAC	4 cut(s)	194, 469, 519, 884
AfiI	CCNNNNNNNGG	3 cut(s)	575, 850, 851
AgsI	TTSAA	3 cut(s)	731, 794, 1012
AjnI	CCWGG	3 cut(s)	473, 942, 1000
AloI	GAACNNNNNNTCC	2 cut(s)	40, 72
AluBI	AGCT	5 cut(s)	12, 350, 808, 835, 839
AluI	AGCT	5 cut(s)	12, 350, 808, 835, 839
Alw26I	GTCTC	3 cut(s)	573, 756, 817
Ama87I	CYCGRG	1 cut(s)	961
Aor13HI	TCCGGA	1 cut(s)	862
AoxI	GGCC	2 cut(s)	70, 696
ApeKI	GCWGC	6 cut(s)	9, 12, 242, 805, 914, 917
ApoI	RAATTY	3 cut(s)	269, 560, 857
Asp700I	GAANNNNTTC	1 cut(s)	783
AspS9I	GGNCC	2 cut(s)	410, 1034
AsuC2I	CCSGG	1 cut(s)	845
AsuNHI	GCTAGC	1 cut(s)	835
AvaI	CYCGRG	1 cut(s)	961
AvaII	GGWCC	2 cut(s)	410, 1034
AxyI	CCTNAGG	1 cut(s)	878
BaeGI	GKGCMC	1 cut(s)	644
BalI	TGGCCA	1 cut(s)	72
BanI	GGYRCC	2 cut(s)	505, 658
BbsI	GAAGAC	1 cut(s)	934
BbvI	GCAGC	5 cut(s)	21, 229, 817, 926, 929
BccI	CCATC	3 cut(s)	159, 434, 844
BciT130I	CCWGG	3 cut(s)	475, 944, 1002
BclI	TGATCA	1 cut(s)	394
BcnI	CCSGG	1 cut(s)	845
BcoDI	GTCTC	3 cut(s)	573, 756, 817
BfaI	CTAG	2 cut(s)	537, 836
BglII	AGATCT	1 cut(s)	131
BisI	GCNGC	6 cut(s)	10, 13, 243, 806, 915, 918
BlsI	GCNGC	6 cut(s)	11, 14, 244, 807, 916, 919
Bme1390I	CCNGG	4 cut(s)	475, 845, 944, 1002
Bme18I	GGWCC	2 cut(s)	410, 1034
BmeT110I	CYCGRG	1 cut(s)	961
BmgT120I	GGNCC	2 cut(s)	410, 1034
BmiI	GGNNCC	4 cut(s)	202, 507, 660, 1025
BmrFI	CCNGG	4 cut(s)	475, 845, 944, 1002
BmrI	ACTGGG	1 cut(s)	775
BmsI	GCATC	1 cut(s)	316
BmtI	GCTAGC	1 cut(s)	839
BmuI	ACTGGG	1 cut(s)	775
BpiI	GAAGAC	1 cut(s)	934
Bpu10I	CCTNAGC	1 cut(s)	1028
BpuEI	CTTGAG	2 cut(s)	677, 1089
BpuMI	CCSGG	1 cut(s)	845
BsaAI	YACGTR	1 cut(s)	486
BsaJI	CCNNGG	3 cut(s)	474, 756, 1000
BsaWI	WCCGGW	1 cut(s)	862
Bsc4I	CCNNNNNNNGG	3 cut(s)	575, 850, 851
Bse1I	ACTGG	5 cut(s)	438, 507, 652, 770, 1071
Bse21I	CCTNAGG	1 cut(s)	878
BseAI	TCCGGA	1 cut(s)	862
BseBI	CCWGG	3 cut(s)	475, 944, 1002
BseDI	CCNNGG	3 cut(s)	474, 756, 1000
BseGI	GGATG	2 cut(s)	151, 307
BseLI	CCNNNNNNNGG	3 cut(s)	575, 850, 851
BseMII	CTCAG	3 cut(s)	24, 260, 1019
BseNI	ACTGG	5 cut(s)	438, 507, 652, 770, 1071
BseSI	GKGCMC	1 cut(s)	644
BseXI	GCAGC	5 cut(s)	21, 229, 817, 926, 929
BsgI	GTGCAG	1 cut(s)	889
BshFI	GGCC	2 cut(s)	72, 698
BshNI	GGYRCC	2 cut(s)	505, 658
BsiHKCI	CYCGRG	1 cut(s)	961
BsiSI	CCGG	3 cut(s)	304, 845, 863
BslFI	GGGAC	1 cut(s)	1073
BslI	CCNNNNNNNGG	3 cut(s)	575, 850, 851
BsmAI	GTCTC	3 cut(s)	573, 756, 817
BsmFI	GGGAC	1 cut(s)	1073
BsnI	GGCC	2 cut(s)	72, 698
BsoBI	CYCGRG	1 cut(s)	961
Bsp1286I	GDGCHC	1 cut(s)	644
Bsp13I	TCCGGA	1 cut(s)	862
Bsp1407I	TGTACA	2 cut(s)	192, 517
Bsp143I	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
BspACI	CCGC	3 cut(s)	177, 638, 1032
BspANI	GGCC	2 cut(s)	72, 698
BspCNI	CTCAG	3 cut(s)	25, 259, 1020
BspEI	TCCGGA	1 cut(s)	862
BspHI	TCATGA	1 cut(s)	493
BspLI	GGNNCC	4 cut(s)	202, 507, 660, 1025
BspOI	GCTAGC	1 cut(s)	839
BspT107I	GGYRCC	2 cut(s)	505, 658
BsrBI	CCGCTC	1 cut(s)	1032
BsrGI	TGTACA	2 cut(s)	192, 517
BsrI	ACTGG	5 cut(s)	438, 507, 652, 770, 1071
BssECI	CCNNGG	3 cut(s)	474, 756, 1000
BssMI	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
BssT1I	CCWWGG	1 cut(s)	756
Bst2UI	CCWGG	3 cut(s)	475, 944, 1002
Bst4CI	ACNGT	3 cut(s)	190, 710, 1016
Bst6I	CTCTTC	2 cut(s)	32, 605
BstAUI	TGTACA	2 cut(s)	192, 517
BstBAI	YACGTR	1 cut(s)	486
BstC8I	GCNNGC	2 cut(s)	640, 837
BstDEI	CTNAG	4 cut(s)	33, 246, 878, 1028
BstF5I	GGATG	2 cut(s)	151, 307
BstKTI	GATC	6 cut(s)	79, 134, 168, 355, 397, 452
BstMAI	GTCTC	3 cut(s)	573, 756, 817
BstMBI	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
BstMWI	GCNNNNNNNGC	1 cut(s)	29
BstNI	CCWGG	3 cut(s)	475, 944, 1002
BstNSI	RCATGY	2 cut(s)	224, 958
BstSCI	CCNGG	4 cut(s)	473, 843, 942, 1000
BstSLI	GKGCMC	1 cut(s)	644
BstSNI	TACGTA	1 cut(s)	486
BstV1I	GCAGC	5 cut(s)	21, 229, 817, 926, 929
BstV2I	GAAGAC	1 cut(s)	934
BstX2I	RGATCY	1 cut(s)	131
BstXI	CCANNNNNNTGG	2 cut(s)	433, 463
BstYI	RGATCY	1 cut(s)	131
Bsu36I	CCTNAGG	1 cut(s)	878
BsuRI	GGCC	2 cut(s)	72, 698
BtsCI	GGATG	2 cut(s)	151, 307
BtsI	GCAGTG	1 cut(s)	757
BtsIMutI	CAGTG	1 cut(s)	757
Cac8I	GCNNGC	2 cut(s)	640, 837
CciI	TCATGA	1 cut(s)	493
Cfr13I	GGNCC	2 cut(s)	410, 1034
Csp6I	GTAC	4 cut(s)	193, 468, 518, 883
CviAII	CATG	8 cut(s)	74, 221, 398, 494, 801, 812, 886, 955
CviQI	GTAC	4 cut(s)	193, 468, 518, 883
DdeI	CTNAG	4 cut(s)	33, 246, 878, 1028
DpnI	GATC	6 cut(s)	78, 133, 167, 354, 396, 451
DpnII	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
EaeI	YGGCCR	1 cut(s)	70
Eam1104I	CTCTTC	2 cut(s)	32, 605
EarI	CTCTTC	2 cut(s)	32, 605
Eco105I	TACGTA	1 cut(s)	486
Eco130I	CCWWGG	1 cut(s)	756
Eco32I	GATATC	2 cut(s)	529, 613
Eco47I	GGWCC	2 cut(s)	410, 1034
Eco57I	CTGAAG	1 cut(s)	1023
Eco81I	CCTNAGG	1 cut(s)	878
Eco88I	CYCGRG	1 cut(s)	961
EcoRI	GAATTC	1 cut(s)	269
EcoRII	CCWGG	3 cut(s)	473, 942, 1000
EcoRV	GATATC	2 cut(s)	529, 613
EcoT14I	CCWWGG	1 cut(s)	756
EcoT22I	ATGCAT	2 cut(s)	403, 673
ErhI	CCWWGG	1 cut(s)	756
FaeI	CATG	8 cut(s)	77, 224, 401, 497, 804, 815, 889, 958
FalI	AAGNNNNNCTT	2 cut(s)	861, 893
FaqI	GGGAC	1 cut(s)	1073
FatI	CATG	8 cut(s)	73, 220, 397, 493, 800, 811, 885, 954
FauI	CCCGC	1 cut(s)	631
FbaI	TGATCA	1 cut(s)	394
Fnu4HI	GCNGC	6 cut(s)	10, 13, 243, 806, 915, 918
FokI	GGATG	2 cut(s)	138, 294
Fsp4HI	GCNGC	6 cut(s)	10, 13, 243, 806, 915, 918
FspBI	CTAG	2 cut(s)	537, 836
GluI	GCNGC	6 cut(s)	10, 13, 243, 806, 915, 918
HaeIII	GGCC	2 cut(s)	72, 698
HapII	CCGG	3 cut(s)	304, 845, 863
Hin1II	CATG	8 cut(s)	77, 224, 401, 497, 804, 815, 889, 958
HincII	GTYRAC	1 cut(s)	687
HindII	GTYRAC	1 cut(s)	687
HinfI	GANTC	2 cut(s)	261, 975
HpaI	GTTAAC	1 cut(s)	687
HpaII	CCGG	3 cut(s)	304, 845, 863
Hpy166II	GTNNAC	3 cut(s)	193, 687, 706
Hpy188I	TCNGA	4 cut(s)	156, 578, 1060, 1077
Hpy188III	TCNNGA	9 cut(s)	80, 232, 344, 494, 728, 863, 950, 961, 963
Hpy8I	GTNNAC	3 cut(s)	193, 687, 706
Hpy99I	CGWCG	2 cut(s)	142, 704
HpyAV	CCTTC	3 cut(s)	812, 883, 1047
HpyCH4III	ACNGT	3 cut(s)	190, 710, 1016
HpyCH4IV	ACGT	2 cut(s)	485, 702
HpyCH4V	TGCA	4 cut(s)	9, 401, 671, 906
HpyF10VI	GCNNNNNNNGC	1 cut(s)	29
HpyF3I	CTNAG	4 cut(s)	33, 246, 878, 1028
HpySE526I	ACGT	2 cut(s)	485, 702
Hsp92II	CATG	8 cut(s)	77, 224, 401, 497, 804, 815, 889, 958
Kpn2I	TCCGGA	1 cut(s)	862
Ksp22I	TGATCA	1 cut(s)	394
KspAI	GTTAAC	1 cut(s)	687
Kzo9I	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
LmnI	GCTCC	3 cut(s)	813, 832, 1023
Lsp1109I	GCAGC	5 cut(s)	21, 229, 817, 926, 929
LweI	GCATC	1 cut(s)	316
MaeI	CTAG	2 cut(s)	537, 836
MaeII	ACGT	2 cut(s)	485, 702
MaeIII	GTNAC	2 cut(s)	158, 1108
MalI	GATC	6 cut(s)	78, 133, 167, 354, 396, 451
MbiI	CCGCTC	1 cut(s)	1032
MboI	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
MboII	GAAGA	9 cut(s)	49, 482, 509, 592, 756, 827, 881, 939, 1000
MflI	RGATCY	1 cut(s)	131
MhlI	GDGCHC	1 cut(s)	644
MlsI	TGGCCA	1 cut(s)	72
MluCI	AATT	7 cut(s)	65, 125, 145, 269, 541, 560, 857
MluNI	TGGCCA	1 cut(s)	72
MlyI	GAGTC	2 cut(s)	270, 969
MmeI	TCCRAC	1 cut(s)	437
MnlI	CCTC	5 cut(s)	33, 167, 671, 701, 859
Mox20I	TGGCCA	1 cut(s)	72
Mph1103I	ATGCAT	2 cut(s)	403, 673
MroI	TCCGGA	1 cut(s)	862
MroXI	GAANNNNTTC	1 cut(s)	783
MscI	TGGCCA	1 cut(s)	72
MseI	TTAA	5 cut(s)	128, 275, 285, 564, 686
MslI	CAYNNNNRTG	1 cut(s)	890
Msp20I	TGGCCA	1 cut(s)	72
MspA1I	CMGCKG	1 cut(s)	12
MspI	CCGG	3 cut(s)	304, 845, 863
MspR9I	CCNGG	4 cut(s)	475, 845, 944, 1002
MvaI	CCWGG	3 cut(s)	475, 944, 1002
MwoI	GCNNNNNNNGC	1 cut(s)	29
NciI	CCSGG	1 cut(s)	845
NdeII	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
NheI	GCTAGC	1 cut(s)	835
NlaIII	CATG	8 cut(s)	77, 224, 401, 497, 804, 815, 889, 958
NlaIV	GGNNCC	4 cut(s)	202, 507, 660, 1025
NsiI	ATGCAT	2 cut(s)	403, 673
NspI	RCATGY	2 cut(s)	224, 958
PaeR7I	CTCGAG	1 cut(s)	961
PagI	TCATGA	1 cut(s)	493
PdmI	GAANNNNTTC	1 cut(s)	783
PkrI	GCNGC	6 cut(s)	11, 14, 244, 807, 916, 919
PleI	GAGTC	2 cut(s)	269, 969
PpsI	GAGTC	2 cut(s)	269, 969
Ppu21I	YACGTR	1 cut(s)	486
Psp6I	CCWGG	3 cut(s)	473, 942, 1000
PspGI	CCWGG	3 cut(s)	473, 942, 1000
PspN4I	GGNNCC	4 cut(s)	202, 507, 660, 1025
PspPI	GGNCC	2 cut(s)	410, 1034
PsuI	RGATCY	1 cut(s)	131
PvuII	CAGCTG	1 cut(s)	12
RsaI	GTAC	4 cut(s)	194, 469, 519, 884
RsaNI	GTAC	4 cut(s)	193, 468, 518, 883
RseI	CAYNNNNRTG	1 cut(s)	890
SaqAI	TTAA	5 cut(s)	128, 275, 285, 564, 686
SatI	GCNGC	6 cut(s)	10, 13, 243, 806, 915, 918
Sau3AI	GATC	6 cut(s)	76, 131, 165, 352, 394, 449
Sau96I	GGNCC	2 cut(s)	410, 1034
SchI	GAGTC	2 cut(s)	270, 969
ScrFI	CCNGG	4 cut(s)	475, 845, 944, 1002
SduI	GDGCHC	1 cut(s)	644
SfaNI	GCATC	1 cut(s)	316
Sfr274I	CTCGAG	1 cut(s)	961
SinI	GGWCC	2 cut(s)	410, 1034
SlaI	CTCGAG	1 cut(s)	961
SmiMI	CAYNNNNRTG	1 cut(s)	890
SmlI	CTYRAG	3 cut(s)	692, 961, 1104
SmoI	CTYRAG	3 cut(s)	692, 961, 1104
SnaBI	TACGTA	1 cut(s)	486
Sse9I	AATT	7 cut(s)	65, 125, 145, 269, 541, 560, 857
SsiI	CCGC	3 cut(s)	177, 638, 1032
SspMI	CTAG	2 cut(s)	537, 836
StyD4I	CCNGG	4 cut(s)	473, 843, 942, 1000
StyI	CCWWGG	1 cut(s)	756
TaaI	ACNGT	3 cut(s)	190, 710, 1016
TaiI	ACGT	2 cut(s)	488, 705
TaqI	TCGA	3 cut(s)	251, 355, 962
TasI	AATT	7 cut(s)	65, 125, 145, 269, 541, 560, 857
TatI	WGTACW	2 cut(s)	192, 517
Tru1I	TTAA	5 cut(s)	128, 275, 285, 564, 686
Tru9I	TTAA	5 cut(s)	128, 275, 285, 564, 686
TscAI	CASTG	1 cut(s)	757
TseI	GCWGC	6 cut(s)	9, 12, 242, 805, 914, 917
TspDTI	ATGAA	6 cut(s)	138, 384, 482, 510, 828, 902
TspGWI	ACGGA	3 cut(s)	155, 254, 1075
TspRI	CASTG	1 cut(s)	757
VpaK11BI	GGWCC	2 cut(s)	410, 1034
XapI	RAATTY	3 cut(s)	269, 560, 857
XceI	RCATGY	2 cut(s)	224, 958
XhoI	CTCGAG	1 cut(s)	961
XmnI	GAANNNNTTC	1 cut(s)	783
XspI	CTAG	2 cut(s)	537, 836
Zsp2I	ATGCAT	2 cut(s)	403, 673

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.