Rorug03G0063500
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000913
Physical Location & Seq
Forward (+)
4717211 .. 4719771
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0063500.1

Sequence Viewer

Length: 1143 bp
ATGGCCACCGTCTCCTCCCCTCCTTTTCAGTTTCCGGTCCCCAAACTCCACCCCGAACCCGACGACTCCGACCCGACCCGACTCCCCACCATCTTCAACCTCTCCTTCAACCAGGACCACGCCTGCTTCGCCGTCGGCACCGACTTCGGCTTCCGCGTCTACAAGTGCGACCCCTTCGCCATCACCATCCGCCGCGACTTCTTCACAGGCGGCGGCTTCGGATTGGTCCGGATACTCAACACCTCCCAAATCTTCGCCATAGTCGGAGGCGGACCCGACCCGGTCTCCCCGGCCAACAGGGTCATGATCTGGGACGACCTCAAGTCCCTCGCCATCCACGACATGTCGTTTCGCTCTGAGGTCAGGGGGCTCAAGCTCCGGAGCGATCGACTCGTCGTCGTTTTGAACCAGAAGATCGTCGTCCACAGCTTCCCTAATTCGAAGCCTCTGCACGAGATACTGACCACGGAGAACCCTAAGGGGCTGTGCGAGGTGTCGCAGAGTCCGGGTCCGATGGTGCTGGCGTGTCCGGGGCTCCATAAGGGTCAAATTCGGGTCGAGAATTACGCATTGAAGCGGACCAAGTTCATAATGGCCCACGATTCCAGTCTCGCCTGCTTTGGGCTCACTCAGGACGGCCGGTATTTGGCTACGGCGAGCAGCAAAGGGACTCTAGTTAGAGTTTTCAGTACTTCAGATGGTACATTACTCCAAGAGGTGAGGAGAGGAGCAGACAGAGCAGAGATTTACAGTCTTGCTTTCTCTTGTACTGGCCAATGGCTAGCTGTGGCTAGTGACAAGGGAACTGTGCATGTTTTCAGTGTAAAGGTTGGTTCAGGATCCATGGAAAAGGATAGATCACGCGGTACATCAGAAGGCGGTGTTCCTAATCCATCAGGCATTTCATCTCTGTCTTTCATCAAAGGTGTGCTGCCAAGGTATTTCAGCTCAGAATGGTCGGTGGCCAAATTTCACTTGCAGGAAGGTCTGCAGCATATTGTTGCCTTTGGCAAGCGGAAGAACACCATCATGATTGTTGGCATGGATGGAAGCTTCTATCGATGCCAATTTGACCCTGTGAATGGAGGAGAAATGACGCAGCTTGAATATCATAACTTCTTGAAGCCAGAAGAAACTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

380

Amino Acids

41.85

Weight (kDa)

8.77

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PROPPIN PF21032 34 - 371 4.8e-28 PROPPIN
Beta-prop_THOC3 PF25174 99 - 279 2.5e-13 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 183 - 277 3.6e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 199 - 278 7e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 137
AccI GTMKAC 1 cut(s) 159
AccII CGCG 3 cut(s) 156, 195, 864
AccIII TCCGGA 2 cut(s) 228, 378
AclWI GGATC 2 cut(s) 834, 847
AcoI YGGCCR 5 cut(s) 3, 291, 637, 772, 963
AcsI RAATTY 2 cut(s) 549, 968
AcuI CTGAAG 1 cut(s) 678
AfaI GTAC 4 cut(s) 691, 703, 769, 868
AfiI CCNNNNNNNGG 3 cut(s) 621, 646, 1082
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 6 cut(s) 97, 109, 406, 574, 1106, 1123
AhdI GACNNNNNGTC 2 cut(s) 322, 395
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 6 cut(s) 376, 429, 785, 948, 1053, 1102
AluI AGCT 6 cut(s) 376, 429, 785, 948, 1053, 1102
Alw26I GTCTC 3 cut(s) 16, 289, 614
AlwI GGATC 2 cut(s) 834, 847
Aor13HI TCCGGA 2 cut(s) 228, 378
AoxI GGCC 6 cut(s) 3, 291, 594, 637, 772, 963
ApeKI GCWGC 4 cut(s) 660, 931, 991, 1099
ApoI RAATTY 2 cut(s) 549, 968
AspS9I GGNCC 7 cut(s) 37, 115, 226, 272, 509, 579, 595
AsuC2I CCSGG 4 cut(s) 281, 290, 507, 531
AsuHPI GGTGA 2 cut(s) 175, 730
AsuII TTCGAA 1 cut(s) 440
AsuNHI GCTAGC 1 cut(s) 781
AvaII GGWCC 6 cut(s) 37, 115, 226, 272, 509, 579
AxyI CCTNAGG 1 cut(s) 477
BalI TGGCCA 3 cut(s) 5, 774, 965
BamHI GGATCC 1 cut(s) 839
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 3 cut(s) 372, 537, 627
BauI CACGAG 1 cut(s) 452
BbvI GCAGC 4 cut(s) 672, 918, 1003, 1111
BccI CCATC 9 cut(s) 98, 188, 194, 341, 508, 692, 901, 1034, 1040
BceAI ACGGC 3 cut(s) 116, 652, 669
BcgI CGANNNNNNTGC 4 cut(s) 127, 161, 430, 464
BciT130I CCWGG 1 cut(s) 113
BciVI GTATCC 1 cut(s) 225
BcnI CCSGG 4 cut(s) 281, 290, 507, 531
BcoDI GTCTC 3 cut(s) 16, 289, 614
BfaI CTAG 3 cut(s) 674, 782, 792
BfmI CTRYAG 1 cut(s) 989
BfuI GTATCC 1 cut(s) 225
BisI GCNGC 7 cut(s) 193, 211, 214, 661, 932, 992, 1100
BlsI GCNGC 7 cut(s) 194, 212, 215, 662, 933, 993, 1101
BmcAI AGTACT 1 cut(s) 691
Bme1390I CCNGG 5 cut(s) 113, 281, 290, 507, 531
Bme18I GGWCC 6 cut(s) 37, 115, 226, 272, 509, 579
BmeRI GACNNNNNGTC 2 cut(s) 322, 395
BmgT120I GGNCC 7 cut(s) 37, 115, 226, 272, 509, 579, 595
BmiI GGNNCC 6 cut(s) 39, 139, 274, 510, 536, 841
BmrFI CCNGG 5 cut(s) 113, 281, 290, 507, 531
BmsI GCATC 1 cut(s) 1052
BmtI GCTAGC 1 cut(s) 785
Bpu14I TTCGAA 1 cut(s) 440
BpuEI CTTGAG 2 cut(s) 305, 356
BpuMI CCSGG 4 cut(s) 281, 290, 507, 531
Bsa29I ATCGAT 1 cut(s) 1060
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 5 cut(s) 288, 465, 530, 843, 935
BsaWI WCCGGW 3 cut(s) 34, 228, 378
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 621, 646, 1082
Bse118I RCCGGY 1 cut(s) 639
Bse1I ACTGG 2 cut(s) 606, 775
Bse21I CCTNAGG 1 cut(s) 477
BseAI TCCGGA 2 cut(s) 228, 378
BseBI CCWGG 1 cut(s) 113
BseCI ATCGAT 1 cut(s) 1060
BseDI CCNNGG 5 cut(s) 288, 465, 530, 843, 935
BseGI GGATG 3 cut(s) 186, 333, 1051
BseLI CCNNNNNNNGG 3 cut(s) 621, 646, 1082
BseMII CTCAG 3 cut(s) 348, 644, 963
BseNI ACTGG 2 cut(s) 606, 775
BseRI GAGGAG 4 cut(s) 4, 736, 741, 1101
BseX3I CGGCCG 1 cut(s) 637
BseXI GCAGC 4 cut(s) 672, 918, 1003, 1111
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 3 cut(s) 156, 195, 864
Bsh1285I CGRYCG 2 cut(s) 388, 640
BshFI GGCC 6 cut(s) 5, 293, 596, 639, 774, 965
BshNI GGYRCC 1 cut(s) 137
BshVI ATCGAT 1 cut(s) 1060
BsiEI CGRYCG 2 cut(s) 388, 640
BsiSI CCGG 8 cut(s) 35, 229, 281, 290, 379, 506, 530, 640
BslFI GGGAC 4 cut(s) 23, 310, 326, 682
BslI CCNNNNNNNGG 3 cut(s) 621, 646, 1082
BsmAI GTCTC 3 cut(s) 16, 289, 614
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 4 cut(s) 23, 310, 326, 682
BsnI GGCC 6 cut(s) 5, 293, 596, 639, 774, 965
Bso31I GGTCTC 1 cut(s) 289
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 372, 537, 627
Bsp13I TCCGGA 2 cut(s) 228, 378
Bsp143I GATC 5 cut(s) 306, 385, 414, 839, 857
Bsp19I CCATGG 1 cut(s) 843
BspANI GGCC 6 cut(s) 5, 293, 596, 639, 774, 965
BspCNI CTCAG 3 cut(s) 349, 643, 962
BspDI ATCGAT 1 cut(s) 1060
BspEI TCCGGA 2 cut(s) 228, 378
BspFNI CGCG 3 cut(s) 156, 195, 864
BspHI TCATGA 2 cut(s) 303, 1029
BspLI GGNNCC 6 cut(s) 39, 139, 274, 510, 536, 841
BspMAI CTGCAG 1 cut(s) 993
BspOI GCTAGC 1 cut(s) 785
BspPI GGATC 2 cut(s) 834, 847
BspT104I TTCGAA 1 cut(s) 440
BspT107I GGYRCC 1 cut(s) 137
BspTNI GGTCTC 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 639
BsrI ACTGG 2 cut(s) 606, 775
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 5 cut(s) 288, 465, 530, 843, 935
BssMI GATC 5 cut(s) 306, 385, 414, 839, 857
BssSI CACGAG 1 cut(s) 452
BssT1I CCWWGG 2 cut(s) 843, 935
Bst2BI CACGAG 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 3 cut(s) 10, 752, 808
BstBI TTCGAA 1 cut(s) 440
BstC8I GCNNGC 6 cut(s) 124, 522, 616, 658, 783, 1013
BstDEI CTNAG 4 cut(s) 357, 477, 630, 949
BstDSI CCRYGG 2 cut(s) 465, 843
BstF5I GGATG 3 cut(s) 186, 333, 1051
BstFNI CGCG 3 cut(s) 156, 195, 864
BstKTI GATC 5 cut(s) 309, 388, 417, 842, 860
BstMAI GTCTC 3 cut(s) 16, 289, 614
BstMBI GATC 5 cut(s) 306, 385, 414, 839, 857
BstMCI CGRYCG 2 cut(s) 388, 640
BstMWI GCNNNNNNNGC 2 cut(s) 128, 737
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 346, 815
BstSCI CCNGG 5 cut(s) 111, 279, 288, 505, 529
BstSFI CTRYAG 1 cut(s) 989
BstUI CGCG 3 cut(s) 156, 195, 864
BstV1I GCAGC 4 cut(s) 672, 918, 1003, 1111
BstX2I RGATCY 1 cut(s) 839
BstYI RGATCY 1 cut(s) 839
BstZI CGGCCG 1 cut(s) 637
Bsu15I ATCGAT 1 cut(s) 1060
Bsu36I CCTNAGG 1 cut(s) 477
BsuI GTATCC 1 cut(s) 225
BsuRI GGCC 6 cut(s) 5, 293, 596, 639, 774, 965
BsuTUI ATCGAT 1 cut(s) 1060
BtgI CCRYGG 2 cut(s) 465, 843
BtsCI GGATG 3 cut(s) 186, 333, 1051
BtsIMutI CAGTG 1 cut(s) 826
Cac8I GCNNGC 6 cut(s) 124, 522, 616, 658, 783, 1013
CciI TCATGA 2 cut(s) 303, 1029
Cfr10I RCCGGY 1 cut(s) 639
Cfr13I GGNCC 7 cut(s) 37, 115, 226, 272, 509, 579, 595
ClaI ATCGAT 1 cut(s) 1060
CseI GACGC 2 cut(s) 145, 1105
Csp6I GTAC 4 cut(s) 690, 702, 768, 867
CviAII CATG 6 cut(s) 304, 343, 812, 844, 1030, 1042
CviQI GTAC 4 cut(s) 690, 702, 768, 867
DdeI CTNAG 4 cut(s) 357, 477, 630, 949
DpnI GATC 5 cut(s) 308, 387, 416, 841, 859
DpnII GATC 5 cut(s) 306, 385, 414, 839, 857
DriI GACNNNNNGTC 2 cut(s) 322, 395
EaeI YGGCCR 5 cut(s) 3, 291, 637, 772, 963
EagI CGGCCG 1 cut(s) 637
Eam1105I GACNNNNNGTC 2 cut(s) 322, 395
EciI GGCGGA 2 cut(s) 179, 285
EclXI CGGCCG 1 cut(s) 637
Eco130I CCWWGG 2 cut(s) 843, 935
Eco24I GRGCYC 3 cut(s) 372, 537, 627
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 6 cut(s) 37, 115, 226, 272, 509, 579
Eco52I CGGCCG 1 cut(s) 637
Eco57I CTGAAG 1 cut(s) 678
Eco81I CCTNAGG 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 2 cut(s) 843, 935
EcoT38I GRGCYC 3 cut(s) 372, 537, 627
ErhI CCWWGG 2 cut(s) 843, 935
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 6 cut(s) 307, 346, 815, 847, 1033, 1045
FaqI GGGAC 4 cut(s) 23, 310, 326, 682
FatI CATG 6 cut(s) 303, 342, 811, 843, 1029, 1041
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 7 cut(s) 193, 211, 214, 661, 932, 992, 1100
FokI GGATG 3 cut(s) 173, 320, 1058
FriOI GRGCYC 3 cut(s) 372, 537, 627
Fsp4HI GCNGC 7 cut(s) 193, 211, 214, 661, 932, 992, 1100
FspBI CTAG 3 cut(s) 674, 782, 792
GluI GCNGC 7 cut(s) 193, 211, 214, 661, 932, 992, 1100
HaeIII GGCC 6 cut(s) 5, 293, 596, 639, 774, 965
HapII CCGG 8 cut(s) 35, 229, 281, 290, 379, 506, 530, 640
HgaI GACGC 2 cut(s) 145, 1105
Hin1II CATG 6 cut(s) 307, 346, 815, 847, 1033, 1045
HindIII AAGCTT 1 cut(s) 1051
HinfI GANTC 6 cut(s) 65, 81, 390, 502, 602, 670
HpaII CCGG 8 cut(s) 35, 229, 281, 290, 379, 506, 530, 640
HphI GGTGA 2 cut(s) 175, 730
Hpy166II GTNNAC 2 cut(s) 160, 424
Hpy188I TCNGA 8 cut(s) 70, 221, 266, 358, 513, 697, 874, 952
Hpy188III TCNNGA 8 cut(s) 229, 304, 379, 559, 632, 837, 1030, 1120
Hpy8I GTNNAC 2 cut(s) 160, 424
Hpy99I CGWCG 5 cut(s) 65, 137, 398, 401, 422
HpyAV CCTTC 4 cut(s) 115, 184, 869, 977
HpyCH4III ACNGT 3 cut(s) 10, 752, 808
HpyCH4V TGCA 4 cut(s) 451, 811, 979, 991
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 737
HpyF3I CTNAG 4 cut(s) 357, 477, 630, 949
Hsp92II CATG 6 cut(s) 307, 346, 815, 847, 1033, 1045
Kpn2I TCCGGA 2 cut(s) 228, 378
Kzo9I GATC 5 cut(s) 306, 385, 414, 839, 857
LmnI GCTCC 4 cut(s) 381, 381, 540, 728
Lsp1109I GCAGC 4 cut(s) 672, 918, 1003, 1111
LweI GCATC 1 cut(s) 1052
MaeI CTAG 3 cut(s) 674, 782, 792
MaeIII GTNAC 1 cut(s) 794
MalI GATC 5 cut(s) 308, 387, 416, 841, 859
MboI GATC 5 cut(s) 306, 385, 414, 839, 857
MboII GAAGA 6 cut(s) 85, 193, 244, 424, 1030, 1142
MflI RGATCY 1 cut(s) 839
MhlI GDGCHC 3 cut(s) 372, 537, 627
MlsI TGGCCA 3 cut(s) 5, 774, 965
MluCI AATT 5 cut(s) 436, 549, 562, 968, 1067
MluNI TGGCCA 3 cut(s) 5, 774, 965
MlyI GAGTC 5 cut(s) 59, 75, 384, 511, 664
MmeI TCCRAC 2 cut(s) 93, 244
Mox20I TGGCCA 3 cut(s) 5, 774, 965
MroI TCCGGA 2 cut(s) 228, 378
MscI TGGCCA 3 cut(s) 5, 774, 965
MslI CAYNNNNRTG 1 cut(s) 1028
Msp20I TGGCCA 3 cut(s) 5, 774, 965
MspI CCGG 8 cut(s) 35, 229, 281, 290, 379, 506, 530, 640
MspR9I CCNGG 5 cut(s) 113, 281, 290, 507, 531
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 3 cut(s) 156, 195, 864
MwoI GCNNNNNNNGC 2 cut(s) 128, 737
NciI CCSGG 4 cut(s) 281, 290, 507, 531
NcoI CCATGG 1 cut(s) 843
NdeII GATC 5 cut(s) 306, 385, 414, 839, 857
NheI GCTAGC 1 cut(s) 781
NlaIII CATG 6 cut(s) 307, 346, 815, 847, 1033, 1045
NlaIV GGNNCC 6 cut(s) 39, 139, 274, 510, 536, 841
NmuCI GTSAC 1 cut(s) 794
NspI RCATGY 2 cut(s) 346, 815
NspV TTCGAA 1 cut(s) 440
PagI TCATGA 2 cut(s) 303, 1029
PciI ACATGT 1 cut(s) 342
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 336
PfeI GAWTC 1 cut(s) 602
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 7 cut(s) 194, 212, 215, 662, 933, 993, 1101
Ple19I CGATCG 1 cut(s) 388
PleI GAGTC 5 cut(s) 59, 75, 384, 510, 664
PpsI GAGTC 5 cut(s) 59, 75, 384, 510, 664
PscI ACATGT 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 6 cut(s) 39, 139, 274, 510, 536, 841
PspPI GGNCC 7 cut(s) 37, 115, 226, 272, 509, 579, 595
PstI CTGCAG 1 cut(s) 993
PsuI RGATCY 1 cut(s) 839
PsyI GACNNNGTC 1 cut(s) 281
PvuI CGATCG 1 cut(s) 388
RsaI GTAC 4 cut(s) 691, 703, 769, 868
RsaNI GTAC 4 cut(s) 690, 702, 768, 867
RseI CAYNNNNRTG 1 cut(s) 1028
SatI GCNGC 7 cut(s) 193, 211, 214, 661, 932, 992, 1100
Sau3AI GATC 5 cut(s) 306, 385, 414, 839, 857
Sau96I GGNCC 7 cut(s) 37, 115, 226, 272, 509, 579, 595
ScaI AGTACT 1 cut(s) 691
SchI GAGTC 5 cut(s) 59, 75, 384, 511, 664
ScrFI CCNGG 5 cut(s) 113, 281, 290, 507, 531
SduI GDGCHC 3 cut(s) 372, 537, 627
SfaNI GCATC 1 cut(s) 1052
SfcI CTRYAG 1 cut(s) 989
SfuI TTCGAA 1 cut(s) 440
SinI GGWCC 6 cut(s) 37, 115, 226, 272, 509, 579
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 2 cut(s) 320, 371
SmoI CTYRAG 2 cut(s) 320, 371
Sse9I AATT 5 cut(s) 436, 549, 562, 968, 1067
SspMI CTAG 3 cut(s) 674, 782, 792
StyD4I CCNGG 5 cut(s) 111, 279, 288, 505, 529
StyI CCWWGG 2 cut(s) 843, 935
TaaI ACNGT 3 cut(s) 10, 752, 808
TaqI TCGA 4 cut(s) 388, 440, 558, 1060
TasI AATT 5 cut(s) 436, 549, 562, 968, 1067
TatI WGTACW 2 cut(s) 689, 767
TauI GCSGC 3 cut(s) 195, 213, 216
TfiI GAWTC 1 cut(s) 602
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 4 cut(s) 660, 931, 991, 1099
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 3 cut(s) 577, 894, 907
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 1 cut(s) 826
Tth111I GACNNNGTC 1 cut(s) 281
VpaK11BI GGWCC 6 cut(s) 37, 115, 226, 272, 509, 579
XapI RAATTY 2 cut(s) 549, 968
XceI RCATGY 2 cut(s) 346, 815
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 3 cut(s) 674, 782, 792
ZrmI AGTACT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.