MD04G1166500.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002183
Physical Location & Seq
Reverse (-)
25682789 .. 25683463
675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1166500.v1.1.491

Sequence Viewer

Length: 675 bp
ATGTCGACTCCGACACCCCCGCCGATCCTCCCGGTCTCTACAACCGCAACAGCCGTCGGCGGCGGCACGGCCCAGTCCCAGGCTCCCATCGCTACCCCCGCCTTCCGCGCCTTCATCAACCACATCTCCGACACCTGCCGCAACGGCCTCGCCCAGCGCCGCCCCTGGTCCGAGCTCCTCGACCGATCCGCCTTCGCCAAGCCGGAATCCTTCTCCGACGCTACCGTCCGCGTCCGCAAGAACTACTCCTACTTCCGCGTCAACTACCTCGCTGTTCTCGCCCTCACCGTTGCTGTTTCTCTCTTCACCCACCCGTTTTCCCTCCTCGTCCTCCTCGGCCTCCTCGCCGCCTGGCTCTTCCTCTACCTCTTCCGCCCCTCCGATCAGCCGCTCGTCATCTTTGGCCGCACGTTCTCTGACACCCAGACCCTCTTGGGCCTCACCGCCCTCTCCGTCTTCGTCGTCTTCCTCACATCCGTTGGATCCGTCCTCATCTCCGCTCTCCTCGTGGGTGCCGCAGTCGTCTTCGCTCACGGCGCCTTTAGGGTTCCCGAGGACCTCTTCCTTGACGAGCAAGAGCCTTCCGCCTCCACCGGCTTCCTCTCCTTCCTCAACGGCGCTGCTTCCAATGTCGCTGCCGCCACATCGCCCGCCGTTGTCGCCGCTCGCGGTTGA

Protein Analysis

225

Amino Acids

23.77

Weight (kDa)

8.97

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 49 - 192 4.5e-52 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AasI GACNNNNNNGTC 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 2 cut(s) 512, 536
AccBSI CCGCTC 3 cut(s) 391, 500, 665
AccI GTMKAC 1 cut(s) 5
AccII CGCG 4 cut(s) 108, 231, 258, 669
AclWI GGATC 4 cut(s) 19, 180, 477, 490
AcoI YGGCCR 1 cut(s) 403
AcyI GRCGYC 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 79
AjnI CCWGG 3 cut(s) 78, 164, 350
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 4 cut(s) 19, 180, 477, 490
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 5 cut(s) 69, 145, 337, 403, 436
ApeKI GCWGC 2 cut(s) 620, 635
AspLEI GCGC 4 cut(s) 110, 159, 539, 620
AspS9I GGNCC 4 cut(s) 70, 168, 436, 556
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 3 cut(s) 277, 298, 433
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 168, 556
BamHI GGATCC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 512, 536
BanII GRGCYC 1 cut(s) 177
BauI CACGAG 1 cut(s) 506
BbsI GAAGAC 3 cut(s) 448, 457, 517
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 607, 622
BccI CCATC 1 cut(s) 95
BceAI ACGGC 6 cut(s) 38, 84, 160, 550, 631, 638
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 3 cut(s) 80, 166, 352
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 40
BfoI RGCGCY 3 cut(s) 160, 540, 621
BfuAI ACCTGC 1 cut(s) 143
BglI GCCNNNNNGGC 1 cut(s) 144
Bme1390I CCNGG 4 cut(s) 32, 80, 166, 352
Bme18I GGWCC 2 cut(s) 168, 556
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 4 cut(s) 70, 168, 436, 556
BmiI GGNNCC 5 cut(s) 84, 484, 514, 538, 549
BmrFI CCNGG 4 cut(s) 32, 80, 166, 352
BmrI ACTGGG 1 cut(s) 67
BmuI ACTGGG 1 cut(s) 67
BpiI GAAGAC 3 cut(s) 448, 457, 517
BpuMI CCSGG 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 40
BsaJI CCNNGG 4 cut(s) 78, 164, 334, 552
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 73
BseBI CCWGG 3 cut(s) 80, 166, 352
BseDI CCNNGG 4 cut(s) 78, 164, 334, 552
BseGI GGATG 1 cut(s) 473
BseLI CCNNNNNNNGG 1 cut(s) 79
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 5 cut(s) 167, 314, 323, 332, 494
BseXI GCAGC 2 cut(s) 607, 622
BseYI CCCAGC 1 cut(s) 153
Bsh1236I CGCG 4 cut(s) 108, 231, 258, 669
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 5 cut(s) 71, 147, 339, 405, 438
BshNI GGYRCC 2 cut(s) 512, 536
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 3 cut(s) 32, 203, 594
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 5 cut(s) 71, 147, 339, 405, 438
Bso31I GGTCTC 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 4 cut(s) 24, 185, 382, 482
BspANI GGCC 5 cut(s) 71, 147, 339, 405, 438
BspFNI CGCG 4 cut(s) 108, 231, 258, 669
BspLI GGNNCC 5 cut(s) 84, 484, 514, 538, 549
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 4 cut(s) 19, 180, 477, 490
BspQI GCTCTTC 1 cut(s) 362
BspT107I GGYRCC 2 cut(s) 512, 536
BspTNI GGTCTC 1 cut(s) 40
BsrBI CCGCTC 3 cut(s) 391, 500, 665
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 1 cut(s) 73
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 4 cut(s) 78, 164, 334, 552
BssMI GATC 4 cut(s) 24, 185, 382, 482
BssNI GRCGYC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 506
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 3 cut(s) 80, 166, 352
Bst4CI ACNGT 2 cut(s) 226, 289
Bst6I CTCTTC 4 cut(s) 308, 362, 374, 566
BstACI GRCGYC 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 651, 667
BstF5I GGATG 1 cut(s) 473
BstFNI CGCG 4 cut(s) 108, 231, 258, 669
BstH2I RGCGCY 3 cut(s) 160, 540, 621
BstHHI GCGC 4 cut(s) 110, 159, 539, 620
BstKTI GATC 4 cut(s) 27, 188, 385, 485
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 4 cut(s) 24, 185, 382, 482
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 7 cut(s) 89, 98, 107, 144, 278, 536, 659
BstNI CCWGG 3 cut(s) 80, 166, 352
BstSCI CCNGG 4 cut(s) 30, 78, 164, 350
BstUI CGCG 4 cut(s) 108, 231, 258, 669
BstV1I GCAGC 2 cut(s) 607, 622
BstV2I GAAGAC 3 cut(s) 448, 457, 517
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 5 cut(s) 71, 147, 339, 405, 438
BtgZI GCGATG 2 cut(s) 73, 630
BtsCI GGATG 1 cut(s) 473
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 651, 667
CfoI GCGC 4 cut(s) 110, 159, 539, 620
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 4 cut(s) 70, 168, 436, 556
CseI GACGC 3 cut(s) 220, 227, 247
DinI GGCGCC 1 cut(s) 538
DpnI GATC 4 cut(s) 26, 187, 384, 484
DpnII GATC 4 cut(s) 24, 185, 382, 482
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 4 cut(s) 308, 362, 374, 566
EarI CTCTTC 4 cut(s) 308, 362, 374, 566
EciI GGCGGA 3 cut(s) 178, 362, 574
Ecl136II GAGCTC 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 168, 556
Eco53kI GAGCTC 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 556
EcoRII CCWGG 3 cut(s) 78, 164, 350
EcoT38I GRGCYC 1 cut(s) 177
EgeI GGCGCC 1 cut(s) 538
EheI GGCGCC 1 cut(s) 538
FaqI GGGAC 1 cut(s) 61
FauI CCCGC 3 cut(s) 27, 106, 658
FblI GTMKAC 1 cut(s) 5
FokI GGATG 1 cut(s) 460
FriOI GRGCYC 1 cut(s) 177
GlaI GCGC 4 cut(s) 109, 158, 538, 619
GsaI CCCAGC 1 cut(s) 157
HaeII RGCGCY 3 cut(s) 160, 540, 621
HaeIII GGCC 5 cut(s) 71, 147, 339, 405, 438
HapII CCGG 3 cut(s) 32, 203, 594
HgaI GACGC 3 cut(s) 220, 227, 247
HhaI GCGC 4 cut(s) 110, 159, 539, 620
Hin1I GRCGYC 1 cut(s) 537
Hin6I GCGC 4 cut(s) 108, 157, 537, 618
HinP1I GCGC 4 cut(s) 108, 157, 537, 618
HincII GTYRAC 2 cut(s) 6, 262
HindII GTYRAC 2 cut(s) 6, 262
HinfI GANTC 2 cut(s) 7, 206
HpaII CCGG 3 cut(s) 32, 203, 594
HphI GGTGA 3 cut(s) 277, 298, 433
Hpy166II GTNNAC 2 cut(s) 6, 262
Hpy188I TCNGA 6 cut(s) 12, 130, 172, 217, 382, 418
Hpy188III TCNNGA 1 cut(s) 551
Hpy8I GTNNAC 2 cut(s) 6, 262
Hpy99I CGWCG 3 cut(s) 59, 221, 464
HpyAV CCTTC 6 cut(s) 112, 121, 202, 220, 591, 616
HpyCH4III ACNGT 2 cut(s) 226, 289
HpyCH4IV ACGT 1 cut(s) 410
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 98, 107, 144, 278, 536, 659
HpySE526I ACGT 1 cut(s) 410
Hsp92I GRCGYC 1 cut(s) 537
HspAI GCGC 4 cut(s) 108, 157, 537, 618
KasI GGCGCC 1 cut(s) 536
Kzo9I GATC 4 cut(s) 24, 185, 382, 482
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 2 cut(s) 88, 180
Lsp1109I GCAGC 2 cut(s) 607, 622
MaeII ACGT 1 cut(s) 410
MalI GATC 4 cut(s) 26, 187, 384, 484
MbiI CCGCTC 3 cut(s) 391, 500, 665
MboI GATC 4 cut(s) 24, 185, 382, 482
MboII GAAGA 7 cut(s) 295, 349, 361, 448, 457, 517, 553
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 1 cut(s) 177
Mly113I GGCGCC 1 cut(s) 537
MmeI TCCRAC 4 cut(s) 35, 153, 240, 460
MspI CCGG 3 cut(s) 32, 203, 594
MspR9I CCNGG 4 cut(s) 32, 80, 166, 352
MvaI CCWGG 3 cut(s) 80, 166, 352
MvnI CGCG 4 cut(s) 108, 231, 258, 669
MwoI GCNNNNNNNGC 7 cut(s) 89, 98, 107, 144, 278, 536, 659
NarI GGCGCC 1 cut(s) 537
NciI CCSGG 1 cut(s) 32
NdeII GATC 4 cut(s) 24, 185, 382, 482
NlaIV GGNNCC 5 cut(s) 84, 484, 514, 538, 549
NmeAIII GCCGAG 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 1 cut(s) 206
PluTI GGCGCC 1 cut(s) 540
PpuMI RGGWCCY 1 cut(s) 556
Psp124BI GAGCTC 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 3 cut(s) 78, 164, 350
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 3 cut(s) 78, 164, 350
PspN4I GGNNCC 5 cut(s) 84, 484, 514, 538, 549
PspPI GGNCC 4 cut(s) 70, 168, 436, 556
PspPPI RGGWCCY 1 cut(s) 556
PsrI GAACNNNNNNTAC 2 cut(s) 233, 265
PsuI RGATCY 1 cut(s) 482
SacI GAGCTC 1 cut(s) 177
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 362
Sau3AI GATC 4 cut(s) 24, 185, 382, 482
Sau96I GGNCC 4 cut(s) 70, 168, 436, 556
ScrFI CCNGG 4 cut(s) 32, 80, 166, 352
SduI GDGCHC 1 cut(s) 177
SetI ASST 6 cut(s) 137, 177, 270, 369, 413, 561
SfoI GGCGCC 1 cut(s) 538
SinI GGWCC 2 cut(s) 168, 556
SspDI GGCGCC 1 cut(s) 536
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 4 cut(s) 30, 78, 164, 350
TaaI ACNGT 2 cut(s) 226, 289
TaiI ACGT 1 cut(s) 413
TaqI TCGA 2 cut(s) 5, 180
TaqII GACCGA 1 cut(s) 198
TfiI GAWTC 1 cut(s) 206
TseI GCWGC 2 cut(s) 620, 635
TspDTI ATGAA 1 cut(s) 103
TspGWI ACGGA 3 cut(s) 442, 466, 475
VpaK11BI GGWCC 2 cut(s) 168, 556
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.